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TW200607858A - Optimized expression of HPV 31 L1 in yeast - Google Patents

Optimized expression of HPV 31 L1 in yeast

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Publication number
TW200607858A
TW200607858A TW093124870A TW93124870A TW200607858A TW 200607858 A TW200607858 A TW 200607858A TW 093124870 A TW093124870 A TW 093124870A TW 93124870 A TW93124870 A TW 93124870A TW 200607858 A TW200607858 A TW 200607858A
Authority
TW
Taiwan
Prior art keywords
hpv31
yeast
polynucleotides
synthetic
protein
Prior art date
Application number
TW093124870A
Other languages
Chinese (zh)
Other versions
TWI334438B (en
Inventor
Kathrin U Jansen
Loren D Schultz
Michael P Neeper
Henry Z Markus
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Priority to TW93124870A priority Critical patent/TWI334438B/en
Publication of TW200607858A publication Critical patent/TW200607858A/en
Application granted granted Critical
Publication of TWI334438B publication Critical patent/TWI334438B/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Synthetic DNA molecules encoding the HPV31 L1 protein are provided. Specifically, the present invention provides polynucleotides encoding HPV31 L1 protein, wherein said polynucleotides are free from internal transcription termination signals that are recognized by yeast. Also provided are synthetic polynucleotides encoding HPV31 L1 wherein the polynucleotides have been codon-optimized for high level expression in a yeast cell. The synthetic molecules may be used to produce HPV31 virus-like particles (VLPs), and to produce vaccines and pharmaceutical compositions comprising the HPV31 VLPs. The vaccines of the present invention provide effective immunoprophylaxis against papillomavirus infection through neutralizing antibody and cell-mediated immunity.
TW93124870A 2004-08-18 2004-08-18 Optimized expression of hpv 31 l1 in yeast TWI334438B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW93124870A TWI334438B (en) 2004-08-18 2004-08-18 Optimized expression of hpv 31 l1 in yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW93124870A TWI334438B (en) 2004-08-18 2004-08-18 Optimized expression of hpv 31 l1 in yeast

Publications (2)

Publication Number Publication Date
TW200607858A true TW200607858A (en) 2006-03-01
TWI334438B TWI334438B (en) 2010-12-11

Family

ID=44211874

Family Applications (1)

Application Number Title Priority Date Filing Date
TW93124870A TWI334438B (en) 2004-08-18 2004-08-18 Optimized expression of hpv 31 l1 in yeast

Country Status (1)

Country Link
TW (1) TWI334438B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021013062A1 (en) * 2019-07-19 2021-01-28 神州细胞工程有限公司 Chimeric human papillomavirus type 31 l1 protein

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021013062A1 (en) * 2019-07-19 2021-01-28 神州细胞工程有限公司 Chimeric human papillomavirus type 31 l1 protein

Also Published As

Publication number Publication date
TWI334438B (en) 2010-12-11

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