TW200410681A - Amino acid compound for improving central function - Google Patents

Abstract

The present invention relates to an amino acid compound or amino acid solution, which consists of threonine(Thr), proline(Pro), glycine(Gly), valine(Val), isoleucine(Ile), leucine(Leu), tyrosine(Tyr), phenylalanine(Phe), lysine(Lys), asparatic acid(Asp), serine(Ser), glutamine(Gln), alanine(Ala), methionine(Met), tryptophan(Trp), histiding(His) and arginine(Arg), used for central administration function. The amino acid compound has no side effect because of comprising the improvement for the central function of the nature central tiredness recovery etc.

Description

200410681 发明 Description of the invention: [Technical field to which the invention belongs] The present invention relates to an amino acid composition and an amino acid composition solution, which have a significant effect on improving central function. More specifically, the invention relates to wasps (Vespas ( V espa)) The saliva secreted by the larva contains an amino acid composition and an amino acid composition solution that can be administered to restore central fatigue. [Prior Art] The authors of the present invention have investigated the saliva secreted by larvae of wasps from the past. In addition to studying the composition of the amino acid composition contained therein, they have repeatedly discussed the use of φφ. As a result, it was found that one of the amino acid compositions named VAAM among most of the amino acids contained in the saliva had hyperkinetic function (Patent No. 2 5 1 86 92 2). This hyperkinetic effect includes sustained muscle strength, nourishing and strengthening, nutritional supplementation, and restoring fatigue. Heparin is a physiologically active amine, which is abundantly contained in intestinal chromaffin cells such as the brain, pineal gland, blood platelet, and intestine, and plays an important role in controlling brain function. The concentration of heparin in the brain is closely related to the function of the brain. In particular, high concentrations can induce fatigue and hypnosis. In addition, interactions with other brain amines such as dopamine cause extremely low levels of dependence. It also has a strong effect on emotional and physical control. Hemoglobin in the brain during human exercise increases with an increase in tryptophan in the blood during exercise. It is speculated that this may lead to increased fatigue during exercise, induced drowsiness, and burnout. Such central fatigue and muscle fatigue will hinder performance improvement during exercise. -6- 200410681 [Summary of the invention] The object of the present invention is to provide an amino acid composition or an amino acid solution that can restore central fatigue and improve central function. The present invention is a kind of glutamic acid, proline acid, glycine acid, valine acid, isoleucine, leucine, tyrosine, phenylalanine, lysine, aspartic acid, serine, The composition of glutamic acid, alanine, methionine, tryptophan, histidine, and arginine can be improved by using amino acid composition. The present invention also includes a solution containing an amino acid composition having the same composition in the liquid phase, and more preferably an aqueous solution. As described below, each amino acid of φ in the above composition or solution has a specific ideal blending ratio. This amino acid composition and its solution are natural sources and have no side effects. Compared with other amino acid compositions, It has a high effect on reducing the concentration of heparin that causes central fatigue, and has a superior central function improvement effect. The amino acid composition that removes one or two or more amino acids in the amino acid composition is not easy to have the above-mentioned central function improving effect, except for the amino acid composition or the amino acid solution that only removes tryptophan. It has a better central function improving effect than the amino acid composition or solution. As will be described later, '41. This amino acid composition or solution, which is more preferable, contains each amino acid in a specific desired blending ratio. The present invention is described in detail below. As mentioned above, VAAM has a complex mechanism of hyperkinesis, which integrates the functions of hyperlipid metabolism, liver function, kidney function, and body temperature. These functions are combined to improve motor function. 1 7- 200410681 From this point of view, we know that in order to improve sports performance, we need to improve the performance. On the other hand, heparin in the brain is the cause of central fatigue. The loss of appetite, sleepiness, burnout, and fatigue caused by exercise is the cause of elevated heparin concentrations. From the change in the amount of heparin, the role of VAAM in the center during exercise is known. One of the VAAMs such as the amino acid composition described above greatly contributes to the recovery of the center. The present inventors have found that if a VAAM having the above-mentioned specific composition is given to an athlete who has experienced central fatigue and whose haematoxin concentration has risen, the haematoxin concentration can eliminate fatigue and complete the present invention. The first amino acid composition (hereinafter referred to as the "first") related to the present invention contains 17 types of amino acids as essential components, and it is desirable to contain each amino acid in each specific group. That is, glutamic acid 2 to 1 5 moles, proline {Morse, 7 ~ 20 moles of glycine, 4 ~ 8 moles of valine, isoleucine mole, leucine 2 ~ 12 moles, tyrosine 1 ~ 9 Moore, Mole phenylalanine, 5 ~ 11 Mole from lysine, 0.1 ~ 5 Mole from aspartic acid, Mole from serine, 0.1 ~ 4 Mole from glutamic acid, Alanine.丨 ~ 12 mol, acid 0.1 ~ 5 mol, tryptophan 〇. 丨 ~ 5 mol, histamine 〇. 丨 ~ 5 spermine 〇. 1 ~ 5 mol is ideal, of which A small amount of other additives such as amino acids, water-soluble vitamins, and citric acid may also be included. The second amino acid composition (hereinafter referred to as the first, containing a specific composition ratio) according to the present invention 16 types of amino acids are essential ingredients for central nervous system due to overuse of the brain, which reduces blood during fatigue. Invention) Proportionally 狻 4 ~ 30 Acids 3 ~ 9: 0 · 5 ~ 5: 0.1-5 Methionamine Mole and He (Such as the class or its 2 invention), it is -8-200410681 except for the tryptophan composition in the amino acid composition of the first invention 'This tryptophan-free amino acid composition also has the same as the first Inventing the same or better central mechanism improves the effect. Moreover, it is preferable that each amino acid is L-amino acid. The amino acid composition of the first invention and the second invention can be ingested directly as a powder or after being dissolved in water to form an aqueous solution. The ingestion method may be a general administration route such as oral administration, rectal administration, intravenous injection, and drip. In the case of oral administration, in addition to the composition, it can be mixed with pharmaceutically acceptable carriers, excipients, diluents and the like to form powders, granules, lozenges, capsules, oral preparations and the like. However, since solid powders and lozenges require φ time for absorption, it is desirable to administer the composition orally. In this case, it is also possible to administer the above solution with appropriate additives such as salts such as sodium chloride, pH adjusters, and chelating agents. When using injections, appropriate buffers and isotonic agents can be added and dissolved in sterilized distilled water before use. There is no particular limitation on the intake time, and it can be taken at any time before and after the occurrence of central fatigue, especially in the state of a solution as an elixir before exercise begins (for example, cold seasoning, powder seasoning, as a medicine to nourish and strengthen or Ideal for nutritional supplements) intake. Xin because of the amino acid composition of the present invention is low toxicity, the amount of administration can be widely set 'may vary depending on the method of administration and purpose of use, usually once 0.5 ~ 5g, more preferably 丨 times 丨 2g , 丨 day 丨 ~ 20 g, more preferably 4 ~ 10 g. When administered or ingested in the form of a solution, 0.5 to 10% by weight of the solution 1 A in In weight or ingested amount is about 10 to 丨 〇〇〇m1, more preferably i to 4 weight / °丨 Valley fluid once administered or ingested 1000 ~ 400 m}. From the examples described below, it can be clearly understood that the amine-9-200410681 base acid composition of the first and second inventions has a significant effect on reducing the concentration of heparin and recovering from central fatigue. Also, because of the amino acid composition The natural amino acid source of the system is low in toxicity and it has extremely effective central function improvement. The amino acid composition of the present invention is preferably used in the form of the above solution, especially an aqueous solution. In this case, the compositions of the first invention and the second invention can be directly dissolved in water to prepare a solution, or each amino acid is dissolved in an individual Water, and then make up the solution in the above ratio. [Embodiment] The following are examples and comparative examples related to the central function improvement test of the amino acid composition of the present invention, but the present invention is not limited thereto. (Example 1) Preparation of VAAM A commercially available amino acid was mixed and dissolved in water to prepare a nutrient solution containing a composition of 17 amino acids (VAAM) having a predetermined composition shown in the column of Example 1 in Table 1. 5 week old ddy male rats (SPF) were divided into 5 groups by exercise experiments, and 1.8% VAAM was orally administered at a ratio of 37.5 / 1/1 / g-weight after 12 hours of feeding in the feeding box. The drug was allowed to stand for 30 minutes, and blood was collected from 5 mice and the brain was removed. A 0.3 g hammer was hung from the tail of each of the remaining mice, and after swimming in a dedicated bath at 35 ° C for 30 minutes, blood was collected directly and the brain was removed. Take out the brain and prepare the test article. Take out the brain before and after swimming to measure its weight directly, and freeze it in liquid nitrogen. Nine times the amount of 6% PCA (over 200410681 chloric acid) and 20 v 1 of BHT (dibutylhydroxytoluene) were added to the frozen brain, and homogenized with a Politeron homogenizer, then Hitachi was used. The manufactured high-speed centrifuge was centrifuged at 4 ° C for 10 minutes at a speed of 10,000 r pm, and 2 μm of the supernatant was taken for freeze-drying. To the dry test article, 100 μl // 0.1 M acetic acid buffer (ρΗ4.5) was added to completely dissolve. The analysis of heparin was performed using a high-speed chromatography (L-6200) manufactured by Hitachi. The absorbance detector (L-4000UV-VIS) manufactured by Hitachi was used to measure the absorption at 2 80nm. A Cromabat recorder manufactured by Shimadzu Corporation was used. Make a record. The column used was Zhao ODS-S i 1 ica (18c-5, 4.6x250mm), mobile phase [J using a Molybdenum containing 10% methanol and 丨 mole EDTA (ethylenediaminetetraacetic acid) Ear acetate buffer (P Η 4 · 5) 'The flow rate was 1.0 m 1 / cm. The measurement results are shown in Fig. 1. (Comparative Examples 1 and 2) CAAM (Comparative Example 1) or distilled water (Comparative Examples 2, DW) shown in Table 1 were used instead of VAAM of Example 1, and administered to mice under the same conditions as in Example 1, and 1 Heparin concentration was measured under the same conditions. The results are shown in Figure 1. Win-11- 200410681 (Table 1) Amino acid (mole%) Examples Comparative Example 1 1 2 VAAM CAAM DW Aspartic acid 0.2 7.5 — Glucine 7.2 2.5 — Serine 2.5 8.0 — Branamine 3.2 19.6 Monoproline 18.0 8.5 Monoglycine 19.1 4.5 Monoalanine 6.0 4.5 Monovaline 5.9 5.5 — Cysteine — 0.4 — Methionine 0.5 2.5 Monoisoleucine 4.5 5.5 — Leucine 6.2 8.5 — Tyrosine 6.0 5.0 — Phenylalanine 3.8 4.0 — Lysine 8.6 7.0 — Tryptophan 2.2 1.0 — Histidine 2.6 2.5 Monosine 3.5 3.0 — 200410681 Related to Example 1 and Comparative Examples 1 to 2 Explore the results of the measurement. The concentration of heparin before swimming was highest in the VAAM administration group of Example 1, the CAAM administration group of Comparative Example 1 was lower, and the DW administration group of Comparative Example 2 was the lowest (refer to Figure 1, in the chart) 5-ΗΤ means heparin). The heparin concentration after swimming was the highest in the CAAM administration group in Comparative Example 1, the DW administration group in Comparative Example 2 was the highest, and the VAAM administration group in Example 1 was the lowest (see Figure 1). Comparing the concentrations of heparin before and after swimming, the concentration of the VAAM administration group in Example 1 decreased the most, and the concentration of the CAAM administration group in Comparative Example 1 decreased less; on the contrary, the concentration of heparin in the DW administration group of Comparative Example 2 rise. The heparin in the brain is directly proportional to the tryptophan concentration in the blood, and the heparin concentration before swimming is the highest in the graph in Figure 丨. The tryptophan content in VAAM is more than twice the tryptophan content in CAAM. The concentration of heparin in the brain after swimming in the VAAM administration group is lower than that in the CAAM administration group. These results clearly show that the VAAM administration group has a specific effect on the brain. The above-mentioned embodiment is described as an example, but the present invention is not limited to the above-mentioned continuous application state 'as long as the modifications and changes can be made by those skilled in the art without departing from the scope of the present invention. [Brief Description of Drawings] Fig. 1 is a graph showing the relationship between VAAM, VAAM-TRP, CAAM, DW, and heparin concentration given in Examples 1 and 2 and Comparative Examples 1 and 2.

Claims (1)

200410681 Scope of patent application: 1 · An amino acid composition or amino acid solution for improving central function, which is characterized by aging amino acid (Thi ·), proline (Pro), and glycine (Giy) , Valine (Val), Isoleucine (Ile), Leucine (Leu), Tyrosine (Tyi), Phenylalanine (Phe), Lysine (Lys), Aspartic acid (Asp) , Serine, Serine, Gin, Ala, Met, T rp, H is and Arginine (Arg). 2 · If the cardinal function can be improved by using the amino acid composition or amino acid solution in the scope of the patent application, where each amino acid is melamine 2-15 mol, proline surface acid 4-30 mol, Glycine 7 ~ 20 moles, Valine 4 ~ 8 moles, Isoleucine 3 ~ 9 moles, Leucine 2 ~ 12 moles, Tyramic acid 丨 ~ 9 moles, Phenylalanine 0.5 ~ 5 moles, lysine 5 ~ 11 moles, aspartic acid 〇1 ~ 5 moles, serine 0.1 ~ 5 moles, glutamate ojm moles, alanine 0.1 ~ 12 moles, a Composition ratio of thiamine 0.1 ~ 5 mole, tryptophan οι ~ 5 mole, histidine 0 · 1 ~ 5 mole, and arginine 0.1 ~ 5 mole. 3. A type of amino acid composition or amino acid solution that does not contain tryptophan, which is characterized by containing glutamic acid, proline acid, glycine, valine acid, and isoleucine , Leucine, tyrosine, phenylalanine, lysine, aspartic acid, serine, glutamine, alanine, methionine, histamine, and arginine. 4. The amino acid composition or amino acid solution that does not contain tryptophan can be used to improve the central function of the amino acid without applying tryptophan, in which each amino acid is melamine, 2-15 molar, proline 4 to 30 moles, 7 to 20 moles of glycine, 4 to 8 moles of valine, 3 to 9 moles of isoleucine, 2 to 12 moles of leucine, 1 to tyrosine 9 -14- 200410681 Mol, phenylalanine 0.5 to 5 moles, lysine 5 to 11 moles, aspartic acid 0.1 to 5 moles, serine 0.1 to 5 moles, glutamine 0.1 to 4 moles of acid, 0.1 to 12 moles of alanine, 0.1 to 5 moles of methionine, 0.1 to 5 moles of histamine and 0.1 to 5 moles of arginine Ear proportion composition. 15-