SU976929A1 - Method of producing lactic serum with hydrolized lactose - Google Patents

Description

(54) METHOD OF PULLOVER OF DAIRY WITH HYDULUSED LACTOSE

one

This invention relates to the dairy industry and is intended for use in the processing of whey.

A known method of converting lactose to glucose and galactose in the presence of the isolated enzyme lactase. For this, Sacchoromyces fragilis yeast is grown on protein-free whey for 10-30 hours. P-galactic dinase is obtained from yeast and used to hydrolyze lactose from dairy products (milk, short), followed by inactivation of enzyme 1.

However, the isolated soluble enzyme preparation is a costly catalyst that significantly affects the cost of the finished product, it is unstable in solutions, and requires complex storage conditions.

The closest in technical essence and achievable positive effect to the proposed is a method of production of whey and with schtrolizirovannogo lactose, involving pasteurization of the serum, cooling it to 30-32 ° C, bringing the pH to an optimum value of 6.9-

7.2, application of Sacchoromyces fragtlis yeast for extracellular enzyme (3-galactosidase followed by serum hydrolysis. According to this method, Saccharomvces fragilis yeast in the form of a culture liquid or wet biomass is revived and increased in not large quantities at 48 The yeast starter obtained in this way is introduced to hydrolyze lacto, (, into the whey. Yeast cells digest the enzyme P-galactoscidase and hydrolyze lactose 121 with it.

However, this method is unacceptable for JJ milk production, yeast buildup for hydrolysis is too laborious and time consuming (their storage is impossible even in the cooled state for more than 24 hours).

The purpose of the invention is to simplify the process 20 and reduce the duration of receipt. the final product.

Claims (2)

The goal is achieved in that in a method involving pasteurization of whey, cooling it, bringing the pH to the optimum value, adding Sacchbromyces fragilis yeast to extract D-galactosidase followed by hydrolysis, after cooling, shorten it into two parts, extract the enzyme from dry Yeast Sacchoromyc fragilis biomass by adding it in an amount of 50-100 mg with an activity of 750 e./G. in 1/4 h of serum and surcharge for 2-2.5 h, and hydrolysis of the remaining serum is carried out with the obtained extract. Pr im. 100 ml of pasteurized sugars are cooled to 32 ° G and; neutralize with disodium phosphate to a pH of 6.8, for which 0.1% disodium phosphate is added. After thorough mixing and checking the pH value in 1/4 part of the prepared serum (25 ml), 75 mg of dry yeast biomass with activity is introduced. 750 units The serum is kept for 2.5 hours at 32 ° C for extraction of / J-haloctosidase. Then add the "from the remaining amount of serum (75 ml o fSi 6.8 and leave it at 32 ° C for 3 s. For hydrolysis of Lactose, which hydrolyzes by 50% or more. At the end of the hydrolysis, the syrup is heated to 65 ° C for inactivation enzyme and cooled to a temperature of sale or storage. The invention makes it possible to use the dry biomass of Sacchoromyces fragitis yeast, which for a long time (up to 2 years) is able to keep the enzyme / S-galactosidase active and, when added to the serum, to contain it in the medium and use it to hydrolyze lactose. The omass is well transported and convenient to use. It can be obtained both at the dairy industry in specialized workshops and at the microbiological industry. Formula of the invention A method for the production of whey with hydrolyzed lactose, providing for quick pasteurization of the whey, cooling it pH adjusted to an optimum value, application of Sacchoromyces fragilis yeast to extract the 0-galactosidase enzyme, followed by serum hydrolysis, characterized in that process and reduce the creeping time of the target product, after cooling the shortening is divided into two parts, extraction of jS-galactosidase is carried out from the dry biomass of Sacchoromyces fragi-Us yeast by adding it in an amount of 50-100 mg with an activity of 750 units / g in 1 / 4 hours of serum and feedstock for 2-2.5 hours, and hydrolysis of the remaining serum is carried out with the obtained extract. Sources of information taken into account in the examination 1. US patent number 2749242, cl. 99-55, published. 1956 2. Complex industrial milk processing. Theses of reports of the regional science and technology conference, September 20-22, 1977), Stavropol, 1977, p. 191-198 (prototype).