SU957835A1 - Method of producing aminoacid and lower peptide mixture - Google Patents

Description

The invention relates to the microbiological industry, in particular, to methods for the preparation of blended, amino acid and lower peptides.

A known method of producing a mixture of amino acids and lower peptides involves boiling the yeast in an acidic solution in the presence of the Ku-2 cation exchanger followed by desorption of the C11 amino acids.

The disadvantage of this method is the low quality of the target product.

The closest technical solution to the proposed technical essence and the achieved effect is a method of obtaining a mixture of amino acids and lower peptides, which provides for autolysis of yeast when heated in the presence of a plasmolyzing and sterilizing agent, followed by purification of autolysis & that by passing it through an anion exchange resin. : According to a known method of purification, autolysis is carried out by passing the auto-ethyate through the weakly basic macroporous anion exchanger Ia-1p and cation-exchange resin KU-2 in hydrogen form, Py-2 amino acid and lower peptides from Ku-2 resin with ammonia, evaporating the eluate and drying the desired product C2.

However, the isolation and purification process of the target product is multistage, which leads to product loss during the isolation process at each stage; long duration of the process of isolation and purification of the target product in general and in particular

The 10 conditions for filtration of autolysate through resin IA-1p at pH 5-6 lead to the inevitable inclusion in the technological stages of repeated sterilizations of autolysate due to the possibility of bacterial identification of solutions containing biologically active substances; in addition, the product contains as an impurity nucleins | le acid up to 1%, then

20 as their content should not exceed 0.5%; the product is contaminated with color Iqi iI substances.

The aim of the invention is to increase the yield of the target product, and 25 also to simplify and reduce the cost of the process. ,

This goal is achieved in that according to the method of obtaining a mixture of 1mino acids and lower peptides, which involves autolysis of yeast when heated in the presence of a plasmolyzing and sterilizing agent, followed by purification of the autolysate by passing it through an anion exchange resin, the autolysate is treated with an alkaline agent to pH 10-13 before purification , filtered, and from anion exchange resins use highly basic anion exchanger APA in OH-form. The essence of the method is that the treatment of the autolysate with an alkaline agent results in the rapid flocculation of high molecular weight protein fragments remaining after zeitrifugation of fine cellular residues. In addition, an increase in pH to a value of 10–13 makes it possible to exclude the sterilization stage, since under these conditions the probability of bacterial contamination is low. The transmission of an alkaline autolysate through a highly basic type A anion exchanger in the OH form allows the separation of nucleotides, oligonucleotides that have not been destroyed during autolysis, and pigment substances. The ion exchange purification is carried out in one step. At the same time, amino acids and lower peptides are not sorbed by anion exchange resin. Example. 250 ml of distilled water are poured into the washed and sterilized apparatus, heated to 50 ° C, loaded with stirring 1 kg of freshly pressed yeast, 1.2 ml of toluene are added. The apparatus is hermetically sealed, heated to 48 ° C and maintained at this temperature for 20 hours at this temperature. Autolysate, containing 58 g / l of amino acids, 20 g / l of lower peptides, 12 g / l of nucleic acids, cell residues 160 g / l and pigment substances, centrifuged at 8000 r / m for 10 min. The precipitate is washed with 320 ml of water and centrifuged again at 8000 for 20 minutes. The supernatant and wash water are combined and treated with a 25% aMNniaKa solution to a pH of 11.0. A precipitate of fine cellular residues, high molecular weight protein fragments, is filtered off on a paper filter after 10 minutes. The filtrate is passed through a column with highly basic anion exchanger APA in OH-form (80 g, d 33 mm, and 350 mm) at a rate of 60 ml / h. Anion exchanger npONttil is 300 ml of ammonia water at pH 11.0, they are added to the solution after the autolysate passes through the anion exchanger, evaporated under vacuum and. dried. For 1 kg of compressed yeast, the yield of atlinic acids from lower peptides is 68 g. Ergosterol and a mixture of nucleic acids can also be obtained from the biomass of rye for this extraction scheme of the target product. The resulting mixture of amino acids and lower peptides contains all essential amino acids, and the ratio of essential amino acids (H) to total nitrogen (O) meets the requirements of high-nutritional mixtures. Determination of the amino acid composition is carried out using an LKB aminoanalyzer - accuracy of 3%. The table gives a comparative description of a mixture of amino acids and lower peptides obtained from known and proposed sprays. The yield of the target product per 1 kg of pressed, yeast, g The content of lower peptides,% The content of nucleic acids,% Ash content,% T ellIoNoNoNoNoNoNoNST tolls, quality. content of essential amino acids, g / 1bg total nitrogen Isoleucine Leucine Phenylalania Tyrosine Tryptophan Methionia Cystincystine

Continuation of the table Thus, the proposed method. This method allows to increase the yield of the target product by 13-26% in comparison with the known product, as well as to reduce the consumption of reagents and the time of product isolation due to a sharp decrease in the concentration of nucleic acids.

Claims (2)

1. USSR author's certificate 355219, cl. C 12 D 13/06, 1972, 2. USSR author's certificate 602543, cl. C 12 D 13/06, 1978.