SU704618A1 - Method of improving reproduction activity of animals and vitality of their progeny - Google Patents

Description

The invention is related to animal husbandry, in particular, to means for increasing the fertility of animals and the viability of their offspring. Among the means used in animal husbandry practice (hormonal preparations: short stallion of mares, gravhormones, gonostogen, gonosterone, vitamin E, etc.) there are no drugs that would, along with the stimulating effect on ovos and sperm genesis, prevent embryonic death of offspring. The literature indicates the possibility of using hormonal drugs, which increase fertility 1. However, the viability of the offspring is often lowered. In addition, they increase the number of cases of anovulatory sex hunting with cystic atresia of the follicles. The use of hormonal drugs is limited, as this affects the sexual function. It is known that phytohormones and their structural analogs have a simulating effect on the function of the reproductive organs of animals 2. However, synthetic analogs of phytohormones possessing high physiological activity have high toxicity for animals, which limits their use for these purposes. The purpose of the invention is to create an effective means for increasing the reproductive activity of animals and the viability of offspring from readily available and cheap synthetic phytohormone with low toxicity. This goal is achieved through the use of o-cresoxyacetate-tris- (2-hydroxyethyl) amlyuni (2-СНзСвН40СН2СОО) (МН (СН2 СН20Н) h) 3. In the study of the effect on the reproductive activity of animals, the drug was used iodine with the cDDI cipher. An important feature of this drug is also the simplicity of its preparation according to the following scheme: O-CHH3SbH4OCH2Cyware + CH2CH2O) 3C2 HS OH (0-CH3SbH4OCH2-COO) CNH (CH2CH20H) 3) The synthesis of the drug was either emitted by heg-ect or 10Joctcctctproctctrotect208ct20cTctHe4H2SHN4OCH2CHH4H2SH2SHN4CH2SHH4H2O3 reagents with a further heating of the reaction mixture to a boiling point, or by fusing o-creoxy sulphate with tris- {2oxyethyl) amine. In both cases, the yield of the target product reaches 95-98%. Example 1. Description of the method of obtaining Sh yreparata; A solution of 149.2 g (1.0 mol) of three c- (2-cc-ethyl) amine in 150 MJtSTiDiOiBoifO ciftftpitf is added to a suspension of 174.5 g (1.05 mol) of o-creo siacetic acid in 200 ml of ethanol. The mixture is heated to the boiling point of ethanol, cooled, 100-150 ml of ethyl alcohol are added, and the mixture is allowed to cool. The precipitated crystalline powder is sucked off, washed with ether (to remove unreacted acid 1 Exit-290 g (92%). Melting point 82-83 ° C. From the mother liquor of the liquor evaporated on a water bath, an additional 25 g of the preparation is obtained. The total yield of the target product 99%. Example 2. The toxic effect of the drug was studied in mice, rats and rabbits when injected into the stomach: The study showed that the drug is maltoxic. For mice mg / kg, mg / kg, LD1oo ZVRr mg / kg .. Repeated administration of the drug (10 days or more) in rats at doses of 50-100 mg / kg increase Their weight and the weight of rat embryos. Dosages of the drug mg / kFW Excessive adverse effects on the cardiovascular, vegetative, central nervous system, respiration, blood pressure, excellent function of HP% Tr 6; 4 sec and increases the rate of reaction of the liver. The indicated This has a mild stimulating effect on bone marrow hemorrhage. The doses of the VDPS 50–10Q mg / kg resulted in STD growth of primordial, ovarian follicles. Other animals that received the drug (males and male beings) were mated to study the possibility of an immediate contraceptive effect and the effect of the drug on fertility rates in subsequent generations. The drug in doses of 50-100 mg / kg does not have a delayed contraceptive effect; it does not cause abortions and gyuda treatments both in subsequent pregnancies and during sexual maturity in the offspring prod- uct from experimental animals in the second and third generations. four - . - -In all cases, the noi-born children turned out to be completely normal, and no cases of carcinogenesis were observed. Addition of VDPS preparation to sperm diluent of bullets and rams at concentrations of 2-5 mg / liter increases the intensity of sperm cell movement for 72-100 hours. At ..,. longer observation, the activity of their movement is somewhat reduced. Example 3. The study of the influence of 1sh drug VDPS on the reproductive activity of animals. Study of the effect of the drug on ovo-and spermatogenesis of pro-dyo on sexually mature mice and rats. For 10 days, animals were injected daily with the drug in the stomach in doses of 50-100-200 mg / kg. After you finish, you will inject the drug. animals were slaughtered. By the number of yellow bodies, embryos were judged, about the dissipation of the fruit. Each troupe had 10 animals, the experiments were carried out 3 times. The positive effect of the drug on the intramuscular development of embryos is illustrated by statistically reliable data. In tab. Figure 1 shows the effect of the VDPS preparation on rats embryo dispersion. The table shows that the preparation in doses of 50-100 mg / kg dramatically reduces the resorption of embryos. Further experiments to study the effect of VDPS on the intrauterine development of embryos have shown that its administration to rats in doses of 50-100-200 mg / kg for 10 days c6 increases the weight and quantity of offspring. In the experiments and control groups, some of the Animals were killed, 11 days after fertilization, the rest of the animals remained until delivery and feeding of offspring. Table 2 presents the results of a triple repetition of experiments on the effect of the VDPS preparation on the intrauterine development of embryos. From tab. 2 shows that the doses of the drug 50-100 mg / kg increased the number and weight of zmbrionov, the maximum fertility rate -. yes and its vitality. Experiments on rollers and minks were carried out. with different diets of fed animals. The mink diet was balanced in tryptophan n nicotinic acid. Rabbit received only dry grain (oats) and water. The doses of the preparation were reduced and amounted to 10–20 mg / kt in opgagayayorhx, and 50–100 mg / kg in experiments on rabbits. The preparation of animals with animal carp mixed with food for 15 days: 10 days before insemination and 5 days after fertilization. In tab. Figure 3 shows the results of a study of the effect of the VDPS preparation on the reproductive activity of rabbits and minks.

57046186

Studies have shown that with a variety of drugs. Care, maintenance, feeding

rabbi animal feed, the drug has rendered. groups of animals were the same. positive effect on the reproductive activity of rabbits at a dose of 50 mg / kg with an unbalanced feeding ration. With a balanced feeding of mink for tristophane and nicotinic acid, the drug increases the fertility rate only in small doses (10 mg / kg). A dose of the drug 20 kg / kg reduces the survival of norec. In addition, studies have shown that the drug has a stimulating effect on embryonic development of iipjf litter of various gestational periods in rats. Table 4 presents the results of the effect of VDLS on the embryonic development of rats. The experiments were also conducted not sows; Yay1votnye received ground grain waste in steamed form. The experimental group of sows in the feed. Add a drug at the rate of 1 g per animal. After 20 days, opium and control sows were seeded. sperm hrka with notoriously known activity. On the 30th day after fertilization, part of the sows were killed for the purpose of determining the embryonic gabel of the offspring, others were left until the moment of farrowing and feeding pigs. In the table. 5 shows the results of the effect of DUA on reproductive activity, sows. . The data in Table 5 show that the VDPS preparation increased the birth rate of pigs by more than 60%, weight by 13%, and survival by 14%. In the experiment on ewes, animals received 300 g of threshing feed per day and fed on winter pastures, the preparation was fed together with feed. According to the experimental conditions, 15 eve before the start of artificial insemination, the preparation was added to the feed. After insemination, the feeding of the drug continued for another 5 days in order to prevent embryonic death. The experiment was carried out on three groups of ewes of the Prekos breed, 5 animals each. The first group of ewes receives the drug: 20 days and 100 mg per day, the second - 200 mg, and a third - 300 mg. Similar control rpjTtnbi ewes received top dressing without the purpose of determining the embryonic mortality of the offspring and the estimated fertility from each control and experimental groups of ewes 2 animals were slaughtered 1.5 months after fertilization. In the three control groups (50 ewes each) out of the 6 slaughtered sheep, 2 sheep were not fertilized. Estimated fertility amounted to 83.3 per 100 ewes, which increased the birth rate in these rpjmnax by 41% from 83.3 to 118, per 100 sheep. The control slaughter of animals from the experimental group showed that a dose of the drug of 200 mg was more effective both in embryo weight and in the intended fertility rate. According to the results of the experiment, this dose of the drug also caused the greatest fertility rate (154 gens on 100 ewes). At the same time, doses of 100 mg and 300 mg caused a slightly lower fertility rate per 100 ovdematok (150-147 gn). Subsequent observations showed that under the same conditions of maintenance, feeding and care in the control groups for 5 months, 13.2% fell in the experimental ones, only 4.6%. In the underperformed groups, the hams were born somewhat larger and more viable than in the controls. Thus, the use of the drug allowed the birth rate to be with active viability. In another round of 810 ewes of the Soviet Merino breed, the control slaughter showed that despite the difficult conditions of the year, 6 sheep were fertilized from 6 slaughtered animals. In the control flock (819 ewes), out of 6 slaughtered animals, 3 sheep were fertilized, and the rest of the ovulation was delayed. Thus, according to the results of laboratory research and production tests, the VDPS preparation significantly increases the fertility and viability of the offspring. The low toxicity of the VDPS preparation, rapid elimination from the body, high biological activity, and also low cost and ease of its preparation indicate the promise of using and incorporating the results of research into animal husbandry to increase the reproductive activity of animals and obtain viable offspring.

Table 2

Table 3 About (control) 25 About (control) 285,, 5

15 1 day bere- 186,0tl5,4 174 ± 11,4 ll, 6tl, l

mennost

10 1-4 days 113.0t9.2 102, ± 7.3 10,, 7

of pregnancy

5 9 day - 60.0f4.5 52 + 4.810,, 6

belt

Spreadsheets

9 5.0il, 6 3 l, 7fO, 9 7 6,2t2,7. 4 3.7 ± 1.8

7 11.7M, 4 1 l, 9fO, 9

Table, 6 10, OtO, 6 40 14.0 "2.0 3 l, 2tO, 7