SU63664A1 - The method of obtaining albumin and other proteins from the blood for technical purposes - Google Patents

Description

The currently existing methods for producing albumin from defibrinated animal blood are based primarily on the essence of blood in one way or another. Naturally, the use of drying to isolate albumin must inevitably be accompanied by some thermal denaturation, which causes a decrease in the percentage of soluble proteins, which affects the quality of, for example, the glue used in the plywood industry.

Bulky and capricious apparatus is necessary for drying the blood, which requires continuous monitoring and careful monitoring of the process, as a result of which the quality of the resulting product often fluctuates, especially with regard to the percentage of soluble proteins.

It is quite natural that the attention of many researchers and inventors was drawn to finding the most accessible method of obtaining albumin under all conditions. its derivatives from defibrinated blood without drying, which made it necessary to turn to chemical methods such as coagulation, sedimentation or chemical bonding. However, attempts to use electrolytes to coagulate and precipitate albumin from defibrinated blood were unsuccessful.

It has been established that for the isolation of albumin from defibrinated blood at normal temperature, a whole range of organic substances, preferably acids of both fat and aromatic series, capable of producing complex or other compounds with albumin, is suitable. This includes all organic tanning agents, sulfonic acids, of which sulfonaphenic acids occupy a special place, as well as a number of others. For practical application, the sulfonaphthenic acids contained in the Petrov contact give particularly good results.

Example 1. To 100 liters of defibrinated blood, at room temperature, about 20 liters of Petrov’s contact (mostly salt or black) are added with a constant displacement. A precipitation reaction occurs over several days, and a loose mass of chemically bound albumin and other proteins is formed at the bottom of the vessel. The precipitate is separated from the liquid, washed well with water while stirring and allowed to stand. Thereafter, the precipitate is separated from the washing liquid by any known method and is briquetted under slight pressure to remove excess water. The resulting cake with moisture content up to 20% can be milled, and the resulting powder can be dried in air without heating.

Water can be finally removed from precipitated albzmin by means of drying with organic solvents, such as alcohol, acetone, etc. In this case, it is possible to obtain prod, ykt in a very fine form. The precipitation of blood proteins, taken as a whole, as defibrinated or separated, is made easier and with less consumption of sulfonaphthenic acids (contact), if the operation of precipitation is carried out at a flow of blood. For this, some acid is injected into the blood. Best results are obtained by consuming sulfuric acid.

Example 2. To 100 liters of defibrinated and separated blood, add 9 liters of a 10% solution of sulfuric acid or an equivalent amount of the same acid of a different concentration, with constant stirring. The blood begins to gel. Then it is poured into it, stirring continuously, 5-6 liters of contact. In a short time, the precipitation reaction ends.

Further treatment of the sediment - as described above.

Since the total protein content is greater in whole blood than in defibrinated one, it is advantageous to use whole blood for precipitation with sulfonaphthenic acids (by contact). The deposition order in this case is the same as in the examples described above, but with the difference that the amount of contact required for the deposition is almost doubled. Accordingly, the yield of the final product also increases at least twice.

The product, formed by the combination of albumin and other blood proteins with sflonaphthenic acids, is insoluble in water, but dissolves in solutions of alkaline and organic bases. It is suitable for making adhesives in the plywood industry, then it can be used in pure form or in a mixture with other substances for the manufacture of plastics and products from them, etc.

Subject invention

A method for producing albumin and other proteins from blood for technical purposes, characterized in that albumin and other proteins are precipitated from whole or defibrinnated and separated blood by exposure to the latter sulphonaphthenic acids (Petrov contact) and the resulting precipitate is separated by separation and preformed in the usual way.