SU57890A1 - Color micrograph method - Google Patents

Description

PR (Natural Metallic Microdrug) It is usually easy to make a conclusion about the nature of its colored parts directly by their appearance. Thus, for example, with a sufficient magnification, green chlorophyll grains on the cut sheet of a KJM sheet are distinguished between red and white blobs. However, the presence of strong selective absorption in the visible region of the spectrum necessary for obtaining noticeable colouration in tint: the layers, both in the organic world and in the inanimate nature, is found relatively. Often, and this botanists and biologists came to resort to artificially dyeing their preparations with special dyes selectively adsorbed by various webs of cut. This kind of dye is associated with the introduction of the drug inside the body, which usually changes in sushi conditions. time, and the great experience of the experimenter, and yet does not always lead to the desired results.

The lack of their own visible staining of drugs due to the fact.

Most of the main strong bands are absorbed in the ultraviolet region of the spectrum. Thus, if the eye was able to see the ult (rafiolet light and retained in this area Tsvetoshush's ability, when observed in a microscope with a quartz optic, he would see such preparations in color.

The subject of the present invention is a color micrograph method, which uses an additive three-color photography method to obtain a color microphotogram, in which at least one of the microphotograms is obtained in ultraviolet iCBerre. This makes it possible, when looking at photograms in xpoiMocKon, to use an increased contrast in the densities of the details of the microdrug, which is located in the ultraviolet part of the spectrum.

The application of this method can be carried out following the method;

On MHiKpocKone with quartz optics, equipped with a quartz monochromator, which allows to change the wavelength of light and used to illuminate the drug, three pictures are taken sequentially from the same spot of the preparation. The resulting images in the form of negatives or POSITIVE prints printed from them are projected onto one screen by three projectors, in front of each of which a photo filter is installed, skipping one of three available colors: red, green and blue.

With exactly 1 picture of images, the screen displays a color map, the color of the elements of which is 01. It is determined by the difference in the blackening of the corresponding places in the pictures and therefore, ceteris paribus, xaipaKTepaM absorption in the preparation. The given scheme for obtaining color photographs is similar to cxeMie image acquisition in the three-color additive method of color photographin and differs from the latter only in that, in this case, the purpose is not to reproduce the actual color of Precious1B, but, on the contrary, the colors in the image are definite and arbitrary. filter filters for rass / matrivapi. For practical work, it is more convenient to obtain color images from three negatives 1 instead of a projection onto the screen using one of the known types of xpOMOCKonoiB used for the same purpose in a color photo.

The choice of wavelengths for shooting is determined by the nature of the absorption of the objects studied. Sometimes it is advisable to make one of the pictures in visible light or in infrared rays; in some cases instead of three shots, it may be limited to two. The filters used when viewing images can be selected arbitrarily, IHO to obtain reproducible results: must be set in advance.

An apparatus for such a color micrograph in ultraviolet light consists of a source of ultraviolet rays — a high-voltage between the metal electrodes of a monochromator using high-voltage optics, a microscope with a quadrant capacitor, an objective and an ocular, a micrographic camera to it and a chromoscope — a separate device for viewing color images.

Photomicrographic camera

it has a cassette for VIZh1Nu1O moving on the corresponding skids parallel to the plane of the plate and secured. emulated in three fiksirosannye gooLiSiimh. Such a copy of the cassette allows three SUBs on one plate. The distance between the centers of the lenses of the chromoscope must be exactly equal to the magnitude of the movement of the cassette when producing two adjacent images. If this condition is exceeded, we can, but having attained one exact match in the chromoscope of three pictures by adjusting the slope of the converging silver mirrors, then using the instrument, do not make this adjustment again each time.

The subject of invention.

A way to color micrographs. used to obtain color. Microscopic photographs of the additive method of a three-color photograph, characterized in that at least one of the micrographs is obtained in ultraviolet light, which makes it possible, when looking at photograms in a chromoscope, to use an increased contrast of the densities of the microparate parts, : place in the ultraviolet spectrum.