SU567136A1 - Method of evaluation of bile acid content of bile - Google Patents
Method of evaluation of bile acid content of bileInfo
- Publication number
- SU567136A1 SU567136A1 SU7602319171A SU2319171A SU567136A1 SU 567136 A1 SU567136 A1 SU 567136A1 SU 7602319171 A SU7602319171 A SU 7602319171A SU 2319171 A SU2319171 A SU 2319171A SU 567136 A1 SU567136 A1 SU 567136A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- bile
- minutes
- bile acids
- room temperature
- sulfuric acid
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 9
- 239000003613 bile acid Substances 0.000 title claims description 8
- 210000000941 bile Anatomy 0.000 title claims description 7
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 title 1
- 238000011156 evaluation Methods 0.000 title 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 3
- 229960000583 acetic acid Drugs 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 3
- 238000005375 photometry Methods 0.000 claims description 2
- 230000001376 precipitating effect Effects 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 238000002798 spectrophotometry method Methods 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Description
1one
Изобретение относитс к медицинской биохимии и может быть использовано дл диагностики при различных заболевани х печени, желудочно-кишечного тракта.The invention relates to medical biochemistry and can be used for the diagnosis of various diseases of the liver, gastrointestinal tract.
Известен способ определени содержани желчных кислот в желчи путем предварительного осаждени белков желчи, обработки оставшихс желчных кислот концентрированной серной кислотой при нагревании и спектрофотометр и и смеси.A known method for determining the content of bile acids in bile by pre-precipitating bile proteins, treating the remaining bile acids with concentrated sulfuric acid with heating and a spectrophotometer and mixtures.
Однако известный способ не обеспечивает высокой точности анализа. Кроме того, процесс вл етс многостадийным.However, the known method does not provide high accuracy analysis. In addition, the process is multistage.
Цель изобретени - повысить точность анализа и устранить многостадийность процесса.The purpose of the invention is to improve the accuracy of the analysis and eliminate the multistage process.
Это достигаетс тем, что желчные кислоты обрабатывают серной кислотой, смешанной с равным объемом 0,1%-ного раствора хлорного железа в лед ной уксусной кислоте, выдерживают смесь при комнатной температуре в течение 30-35 мин, затем нагревают в течение 19-21 мин при 60±1°С после охлаждени до комнатной температуры подвергают фотометрии при длине волны 385±1 нм.This is achieved by treating bile acids with sulfuric acid mixed with an equal volume of a 0.1% solution of ferric chloride in glacial acetic acid, keeping the mixture at room temperature for 30-35 minutes, then heating for 19-21 minutes at 60 ± 1 ° C after cooling to room temperature, subjected to photometry at a wavelength of 385 ± 1 nm.
Способ осуществл ют следующим образом.The method is carried out as follows.
В пробирку отмеривают 4,5 мл этилового спирта и 0,5 мл желчи, леремещивают и центрифугируют при 1500 об/мин в течение 5 мин. К 0,1 мл спиртового раствора желчи добавл ют 3,5 мл реактива, представл ющего 0,1%4.5 ml of ethanol and 0.5 ml of bile are metered into the tube, removed and centrifuged at 1500 rpm for 5 minutes. To a 0.1 ml alcohol solution of bile was added 3.5 ml of a reagent representing 0.1%.
раствора хлорного железа в лед ной уксусной кислоте с равным объемом концентрированной серной кислоты, и перемещивают. Через 30 мин пробирку с исследуемой смесью нагревают в течение 20 мин при 60°С. После охлаждени до комнатной температуры фотометрически определ ют содержание желчных кислот при длине волны 385 нм. Расчет концентрации провод т ио калибровочному графику.a solution of ferric chloride in glacial acetic acid with an equal volume of concentrated sulfuric acid, and transferred. After 30 minutes, the test tube with the test mixture is heated for 20 minutes at 60 ° C. After cooling to room temperature, the content of bile acids is determined photometrically at a wavelength of 385 nm. The concentration calculation is performed on a calibration graph.
Предлагаемый способ обладает высокой точностью определени и простотой выполнени .The proposed method has high accuracy of determination and ease of implementation.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7602319171A SU567136A1 (en) | 1976-01-23 | 1976-01-23 | Method of evaluation of bile acid content of bile |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7602319171A SU567136A1 (en) | 1976-01-23 | 1976-01-23 | Method of evaluation of bile acid content of bile |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU567136A1 true SU567136A1 (en) | 1977-07-30 |
Family
ID=20647180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU7602319171A SU567136A1 (en) | 1976-01-23 | 1976-01-23 | Method of evaluation of bile acid content of bile |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU567136A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103282765A (en) * | 2011-01-04 | 2013-09-04 | 皇家飞利浦电子股份有限公司 | An apparatus for optical analysis of an associated tissue |
-
1976
- 1976-01-23 SU SU7602319171A patent/SU567136A1/en active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103282765A (en) * | 2011-01-04 | 2013-09-04 | 皇家飞利浦电子股份有限公司 | An apparatus for optical analysis of an associated tissue |
| CN103282765B (en) * | 2011-01-04 | 2016-08-10 | 皇家飞利浦电子股份有限公司 | Device for optical analysis of associated tissue |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fowden | The quantitative recovery and colorimetric estimation of amino-acids separated by paper chromatography | |
| Peterson | Improved spectrophotometric procedure for determination of serum iron using 4, 7-diphenyl-1, 10-phenanthroline | |
| Edwards et al. | Modified assay for determination of hydroxyproline in a tissue hydrolyzate | |
| Carr et al. | Simplified rapid technic for the extraction and determination of serum cholesterol without saponification | |
| McDermott et al. | Brain-aluminium concentration in dialysis encephalopathy | |
| Hoffman et al. | An improved colorimetric method for the determination of meprobamate in biological fluids | |
| JPS5815738B2 (en) | A method for producing sebum using the sebum extract | |
| Weltman et al. | Fluorescence polarization of human γG-immunoglobulins | |
| Whitehead et al. | A method for the determination of glutamine in cerebrospinal fluid and the results in hepatic coma | |
| CN106367471B (en) | Kit and method for determining total cholesterol | |
| Neidig et al. | Simultaneous estimation of threonine and serine | |
| McChesney | Colorimetric micromethod for determination of antimony in biological materials with concomitant determination of bismuth | |
| SU567136A1 (en) | Method of evaluation of bile acid content of bile | |
| IKEUCHI et al. | A colorimetric determination of boron in biological materials | |
| Snapper et al. | Quantitative aspects of benzoyl glucuronate formation in normal individuals and in patients with liver disorders | |
| Vahouny et al. | Determination of 3-β-hydroxy sterols with anthrone reagent | |
| SU569298A3 (en) | Method of evaluation of steroid concentration | |
| Perrin | The determination of phylloerythrin in blood | |
| Kingsley et al. | Investigation of nuclear fast red method of Baar for direct spectrophotometric determination of calcium in serum, urine, and spinal fluid | |
| Slack | Nitrogenous constituents of the potato | |
| Julshamn et al. | A routine method for the determination of aluminium in human tissue samples using standard addition and graphite furnace atomic absorption spectrophotometry | |
| Verheyden et al. | The estimation of fatty acid esters in serum by the hydroxamate method | |
| US4211531A (en) | Colorimetric cholesterol assay | |
| CN118604229A (en) | Quality detection method and application of donkey-hide gelatin | |
| Speck | Spectrophotometric Determination of Diacetyl |