SU552538A1 - Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations - Google Patents
Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparationsInfo
- Publication number
- SU552538A1 SU552538A1 SU1956266A SU1956266A SU552538A1 SU 552538 A1 SU552538 A1 SU 552538A1 SU 1956266 A SU1956266 A SU 1956266A SU 1956266 A SU1956266 A SU 1956266A SU 552538 A1 SU552538 A1 SU 552538A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- leukocyte
- cationic protein
- detecting free
- microscopic preparations
- microscopic
- Prior art date
Links
- 210000000265 leukocyte Anatomy 0.000 title claims description 5
- 125000002091 cationic group Chemical group 0.000 title claims description 4
- 238000000034 method Methods 0.000 title claims description 3
- 102000004169 proteins and genes Human genes 0.000 title claims description 3
- 108090000623 proteins and genes Proteins 0.000 title claims description 3
- 238000002360 preparation method Methods 0.000 title claims 2
- 230000001086 cytosolic effect Effects 0.000 title description 2
- 238000010186 staining Methods 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 claims 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims 1
- YDIKCZBMBPOGFT-PWUSVEHZSA-N Malvidin 3-galactoside Chemical compound [Cl-].COC1=C(O)C(OC)=CC(C=2C(=CC=3C(O)=CC(O)=CC=3[O+]=2)O[C@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)=C1 YDIKCZBMBPOGFT-PWUSVEHZSA-N 0.000 claims 1
- PXUQTDZNOHRWLI-QOPOCTTISA-O Primulin Natural products O(C)c1c(O)c(OC)cc(-c2c(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O3)cc3c(O)cc(O)cc3[o+]2)c1 PXUQTDZNOHRWLI-QOPOCTTISA-O 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 3
- 238000009534 blood test Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Description
33
лейкоцита. Установив изображение лейкоцита в области фотометрической диафрагмы, снимают показани гальванометра (в условных ед.). Подобным образом фотометрируют 100 клеток, после чего вычисл ют среднее арифметическое интенсивности флуоресценции (в условных ед.) на одну клетку. Так как при этом получают информацию о количестве катионного белка в условных единицах, полученные данные сравнивают с данными исследовани крови контрольной пробы.leukocyte count. Having established the image of a leukocyte in the area of a photometric diaphragm, take readings of a galvanometer (in conditional units). 100 cells are photometrically similarly, after which the arithmetic average of the fluorescence intensity (in units) per cell is calculated. Since this provides information on the amount of cationic protein in arbitrary units, the data obtained are compared with the blood test data from the control sample.
Предлагаемый способ позвол ет быстро определить содержание цитоплазматического катионного белка в лейкоцитах, что вл етс важным показателем функционального состо ни этих клеток.The proposed method allows rapid determination of the cytoplasmic cationic protein content in leukocytes, which is an important indicator of the functional state of these cells.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1956266A SU552538A1 (en) | 1973-07-31 | 1973-07-31 | Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1956266A SU552538A1 (en) | 1973-07-31 | 1973-07-31 | Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU552538A1 true SU552538A1 (en) | 1977-03-30 |
Family
ID=20563675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1956266A SU552538A1 (en) | 1973-07-31 | 1973-07-31 | Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU552538A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5597688A (en) * | 1992-03-27 | 1997-01-28 | Ortho Diagnostic Systems Inc. | Cell fixative and method of analyzing virally infected cells |
| WO1998003875A1 (en) * | 1996-07-24 | 1998-01-29 | Helena Laboratories Corporation | Reagents and methods for fluorescent analysis of serum proteins |
-
1973
- 1973-07-31 SU SU1956266A patent/SU552538A1/en active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5597688A (en) * | 1992-03-27 | 1997-01-28 | Ortho Diagnostic Systems Inc. | Cell fixative and method of analyzing virally infected cells |
| WO1998003875A1 (en) * | 1996-07-24 | 1998-01-29 | Helena Laboratories Corporation | Reagents and methods for fluorescent analysis of serum proteins |
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