SU552353A1 - The method of growing microorganisms - Google Patents
The method of growing microorganismsInfo
- Publication number
- SU552353A1 SU552353A1 SU2098678A SU2098678A SU552353A1 SU 552353 A1 SU552353 A1 SU 552353A1 SU 2098678 A SU2098678 A SU 2098678A SU 2098678 A SU2098678 A SU 2098678A SU 552353 A1 SU552353 A1 SU 552353A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- microorganisms
- yeast
- growing microorganisms
- electrostatic field
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 5
- 244000005700 microbiome Species 0.000 title claims description 5
- 230000005686 electrostatic field Effects 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 230000005684 electric field Effects 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000222178 Candida tropicalis Species 0.000 description 1
- 241000187432 Streptomyces coelicolor Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
туру направл ют на выращивание известными способами.The tour is directed to cultivation by known methods.
Напр женность электрического пол регулируют изменением величины подаваемого напр жени .The intensity of the electric field is controlled by varying the magnitude of the applied voltage.
Пример 1. Воздействию электростатического пол напр женностью 6 кв/см при времени обработки 5 мин подвергали суспензию кормовых дрожжей Candida tropicalis штамм СК-4. Контролем служили дрожжи, не подвергавшиес электрообработке. Культивирование проводили на качалке при 180 об/мин и 29-30°С. Количество выросших дрожжевых клеток определ лось в камере Гор ева непосредственным подсчетом клеток под микроскоиом .Example 1. An electrostatic field with a strength of 6 kV / cm at a treatment time of 5 minutes was subjected to suspension of fodder yeast Candida tropicalis strain SK-4. Yeast that was not subjected to electrotreatment served as control. Cultivation was carried out on a rocking chair at 180 rpm and 29-30 ° C. The number of grown yeast cells was determined in the Gorev chamber by direct counting of cells under a microscope.
Таблица 1Table 1
Динамика роста дрожжевых клеток показана в табл. 1.Dynamics of growth of yeast cells is shown in table. one.
Пример 2. Воздействию электростатического пол напр женностью 7 кв/см подвергали гомогенезированную споровую суспензию культуры Actinomyces coelicolor. Обработанную в электростатическом поле и разведенную суспензию актиномицетов высевали в чашки Петри поверхностным способом на агаризованную питательную среду с последуюш,им прорашиванием в термостатах при 27-28°С. Количество выросших клеток подсчитывали через 7 суток. Эффект электрообработки виден из данных табл. 2.Example 2. An electrostatic field of 7 kV / cm was subjected to a homogenized spore suspension of an Actinomyces coelicolor culture. Processed in an electrostatic field and diluted suspensions of actinomycetes were sown in Petri dishes by surface method on agar nutrient medium followed by sprouting in thermostats at 27-28 ° C. The number of grown cells was counted after 7 days. The effect of electromachining is visible from the data table. 2
Предлагаемый способ активировани выращивани микроорганизмов был апробирован в производственных услови х с помошью опытного образца установки (см. фиг. 2).The proposed method for activating the cultivation of microorganisms was tested under production conditions with the help of a prototype plant (see Fig. 2).
Если по прин той технологии биомасса дрожжей в большой дрожжанке увеличивалась на 15 г/л или на 8,6% (с 175 до 190 г/л), то после электрообработки - на 47 г/л или на 34,5% (с 136 до 183 г/л). В дрожжерастительном чане ио прин той технологии биомасса увеличивалась на 27 г/л или на 18% (с 150 до 177 г/л), а при электрообработке - на 53 г/л или на 35,2% (с 151 до 204 г/л).If, according to the technology adopted, the yeast biomass in a large yeast increased by 15 g / l or by 8.6% (from 175 to 190 g / l), after electrical treatment it increased by 47 g / l or by 34.5% (from 136 up to 183 g / l). In the yeast-growing tank of adopted technology, biomass increased by 27 g / l or 18% (from 150 to 177 g / l), and during electrical treatment, by 53 g / l or 35.2% (from 151 to 204 g / l).
Таблица 2table 2
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2098678A SU552353A1 (en) | 1975-01-16 | 1975-01-16 | The method of growing microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2098678A SU552353A1 (en) | 1975-01-16 | 1975-01-16 | The method of growing microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU552353A1 true SU552353A1 (en) | 1977-03-30 |
Family
ID=20608041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU2098678A SU552353A1 (en) | 1975-01-16 | 1975-01-16 | The method of growing microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU552353A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989003876A1 (en) * | 1987-10-21 | 1989-05-05 | Biosyn-R Corporation | Method for producing cells |
| DE4142244A1 (en) * | 1991-12-20 | 1993-06-24 | Tramm Werner Sabine Dipl Biol | Acceleration of enzymatic processes - by exposing the enzyme substrate combination to alternating electric field of fixed or variable frequency |
| US5888791A (en) * | 1996-01-31 | 1999-03-30 | Ipr Institute For Pharmaceutical Research Ag | Method of producing bacteriorhodopsin and carotenoids by electrostatic treatment of Halobacterium halobium |
-
1975
- 1975-01-16 SU SU2098678A patent/SU552353A1/en active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989003876A1 (en) * | 1987-10-21 | 1989-05-05 | Biosyn-R Corporation | Method for producing cells |
| DE4142244A1 (en) * | 1991-12-20 | 1993-06-24 | Tramm Werner Sabine Dipl Biol | Acceleration of enzymatic processes - by exposing the enzyme substrate combination to alternating electric field of fixed or variable frequency |
| US5888791A (en) * | 1996-01-31 | 1999-03-30 | Ipr Institute For Pharmaceutical Research Ag | Method of producing bacteriorhodopsin and carotenoids by electrostatic treatment of Halobacterium halobium |
| US6238663B1 (en) | 1996-01-31 | 2001-05-29 | Institute Of Pharmaceutical Research Ag | Method for treating hyperkeratotic disease with a Halobacterium halobium lysate |
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