SU368528A1 - METHOD OF ANALYSIS OF ELECTROPROGRAMM - Google Patents

Description

one

The present invention relates to the field of instrumentation and can be used in the design and manufacture of devices for analyzing line spectra obtained, for example, by electrophoresis of whey protein in an acrylamide gel.

Electroforming, for example, whey protein (These are spectra consisting of certain lines. The analysis of such spectra is carried out mainly by visual comparison of the studied and reference electrophoregram.

The disadvantages of a visual comparative analysis of electrophoregrams are very labor intensive and not objective.

The aim of the invention is to develop such a method for analyzing electrophoregrams, which will facilitate and accelerate the obtaining of objective and clear information about the presence of deviations in the spectrum of the studied electrophoregram from the norm, simplify the interpretation of these deviations.

According to the invention, the goal is achieved by comparing the analyzed electrophoregram with the reference one by means of translucent positivity of the analyzed electropherogram recorded on a transparent base with a light source, the brightness of which is modulated across the field by the reference electrophoregram. In this case, the difference picture of the distribution of the spectral lines of the electrophoregram is projected on the screen and can be photographed or marked visually.

Simplification of interpretation of abnormalities in the spectrum of the studied electrophoregram from the norm is achieved by successive comparison of the studied electrophoregram with

a set of similar electrophoregrams of this type, which characterize both normal and previously known pathological changes.

The essence of the proposed electrophoregram analysis method is illustrated in a drawing, in

which depicted the scheme of the device implementing the method.

The device contains a movable clamp / electrophoregram under investigation, an optical projection system 2 with a source

light, a mirror reflector and condenser optics, a cassette 3 with a mobile mechanism for photographic film with a negative image of reference samples, lenses 4, 5 and a projection screen 6.

The electrophoregram 7 under study is placed in a fixative, after which an image of the corresponding reference electrophoregram is projected onto it using a projection system, the negative of which is placed in

cassette 3.

By moving the lens 4 of the projection system and the latch / combine the reference lines of the reference and test electropherogram M samples. At the same time, a differential pattern is obtained on the Garcion screen, in which the presence or absence of those different lines in the sample under study is clearly expressed compared to the reference one. Those lines that are in the test sample, but not in the reference one, will be dark, and those that are not in the test sample, but in the reference one, will be light. The lines that are in both samples and have the same density, in the difference pattern almost disappear - they give a gray background, and the lines with different optical density have a brightness proportional to their differences.

Electrophoregram analysis is greatly simplified if the electrophoretetram under investigation is consistently compared with an aborum of the same type of electrophoregram characterizing both the norm and various pathological changes. For this purpose, the device provides for the possibility of prompt replacement of reference samples of electrophoregrams with known pathological defects. This makes it possible for each electrophoregram under study to select its prototype of a reference sample, i.e., immediately determine the nature of the pathological change characterized by the content of the electropherogram under study.

Subject invention

Method electrophoretic analysis using standard electrophoretic, characterized in that in order to accelerate the obtaining information about a deviation in the analyzed spectrum foregrammy relatively syektra reference electrophoretogram, positive analyzed electrophoretogram image recorded on a transparent basis, shine light flux pro.modulirovanschym lo Toll negative image reference electrophoregrams. and fix the difference picture of the distribution of spectral electrophoregrams.