SU306669A1 - Erythromycin purification method - Google Patents
Erythromycin purification methodInfo
- Publication number
- SU306669A1 SU306669A1 SU6901352764A SU1352764A SU306669A1 SU 306669 A1 SU306669 A1 SU 306669A1 SU 6901352764 A SU6901352764 A SU 6901352764A SU 1352764 A SU1352764 A SU 1352764A SU 306669 A1 SU306669 A1 SU 306669A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- erythromycin
- purification method
- precipitate
- buffer extract
- purification
- Prior art date
Links
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 title claims description 12
- 238000000034 method Methods 0.000 title claims description 6
- 229960003276 erythromycin Drugs 0.000 title claims description 5
- 238000000746 purification Methods 0.000 title claims description 4
- 239000002244 precipitate Substances 0.000 claims description 5
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 claims description 3
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 claims description 3
- 230000021962 pH elevation Effects 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 description 2
- 235000011151 potassium sulphates Nutrition 0.000 description 2
- XTSSJGRRFMNXGO-YZPBMOCRSA-N (3r,4s,5s,6r,7r,9r,11r,12r,13s,14r)-6-[(2s,3r,4s,6r)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy-14-ethyl-7,12,13-trihydroxy-4-[(2r,4r,5s,6s)-5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl]oxy-3,5,7,9,11,13-hexamethyl-oxacyclotetradecane-2,10-dione;sulfu Chemical compound OS(O)(=O)=O.O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 XTSSJGRRFMNXGO-YZPBMOCRSA-N 0.000 description 1
- 229930006677 Erythromycin A Natural products 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000001120 potassium sulphate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Saccharide Compounds (AREA)
Description
Изобретение относитс к медицине, а (имеШГо к методам получени антибиотиков; и касаетс способов очистки эритромицин аThis invention relates to medicine as well (specifically to methods for producing antibiotics; and concerns methods for cleaning erythromycin a
Известен способ очистки эритроми- к цина путем обработки буферного экстракта canbtok Указанный способ, предусматривакицнй использование в качестве соли хлорцстый натрий, не позвол ет получить препарат достаточно высо- jp кой активности и с удовлетворительным качеством.The known method of purification of erythromycin to cin by treating the canbtok buffer extract. This method, involving the use of sodium chloride as a salt, does not allow obtaining the preparation with sufficiently high jp activity and with satisfactory quality.
Цель изобретени - повышение качества очистки эритромицина. Дл этого к буферному экстракту добавл ют суль- 55 фат кали , осадок отдел ют, суспендируют в органическом растворителе, например бутилацетате, и осаждают эритромицин-основание подщелачиванием до рнThe purpose of the invention is to improve the quality of erythromycin purification. For this, potassium sulfate is added to the buffer extract, the precipitate is separated, suspended in an organic solvent, for example butyl acetate, and the erythromycin base is precipitated by alkalinization to pH
около 6,5.20about 6.5.20
Пример. К 320 мл буферного экстракта , содержащего 16 мл бутиладетата, с активностью 41000 ед/мп добавл ют сернокислый калий и после перемешивани в течение 1 ч. отфильтровывают 25 выпавший осадок сульфата эритромицина..Example. Potassium sulphate is added to 320 ml of buffer extract containing 16 ml of butiladetate, with an activity of 41,000 units / mp, and after stirring for 1 hour, 25 erythromycin sulfate precipitate is filtered off.
.Этот осадок суспендируют в 200 ьш бутилацетата и затем расвор ют в 450 мл холодной дистиллированной воды. Водный раствор промывают 2 раза по 15 мл петролейного эфира и осаждают основание эритромицина добавлением водного раствора аммиака до рК 6,-5 при температуре 4-6°С.После нагревани до 50 С отфил.тровывают осадок антибиотика, высушивают и получают 12,2 г препарата (84% от содержани в буферном экстракте ) с активностью 920 ед/мг.This precipitate is suspended in 200% butyl acetate and then dissolved in 450 ml of cold distilled water. The aqueous solution is washed with 2 times 15 ml of petroleum ether and the erythromycin base is precipitated by adding an aqueous solution of ammonia to pK 6, -5 at 4-6 ° C. After heating to 50 ° C, the precipitate of the antibiotic is dried, and 12.2 g are obtained drug (84% of the content in the buffer extract) with an activity of 920 units / mg.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU6901352764A SU306669A1 (en) | 1969-06-30 | 1969-06-30 | Erythromycin purification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU6901352764A SU306669A1 (en) | 1969-06-30 | 1969-06-30 | Erythromycin purification method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU306669A1 true SU306669A1 (en) | 1977-10-25 |
Family
ID=20446891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU6901352764A SU306669A1 (en) | 1969-06-30 | 1969-06-30 | Erythromycin purification method |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU306669A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1266905A4 (en) * | 2000-03-22 | 2003-05-07 | Chugai Pharmaceutical Co Ltd | Process for producing purified erythromycin |
-
1969
- 1969-06-30 SU SU6901352764A patent/SU306669A1/en active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1266905A4 (en) * | 2000-03-22 | 2003-05-07 | Chugai Pharmaceutical Co Ltd | Process for producing purified erythromycin |
| US6767998B2 (en) | 2000-03-22 | 2004-07-27 | Chugai Seiyaku Kabushiki Kaisha | Method for preparing purified erythromycin |
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