SU195036A1 - DEVICE FOR AUTOMATIC STERIOMETRIC MANIPULATIONS OF MICROELECTRODS - Google Patents

Description

Devices for the automatic stereometric manipulation of microelectrodes in an experiment, comprising a device for securing the test animal and a microelectrode with a fixing mechanism, are known.

The proposed device differs from the known ones in that it has an orthogonal manipulator, made in the form of three series-connected actuating elements, for example, hydraulic actuating cylinders. In addition, each post-cylinder is rigidly fixed on the piston rod of the previous one, and the electrode is rigidly fixed on the piston rod of the last, third, cylinder. Due to this, the microelectrode automatically moves along the surface of the brain of the studied animal and in its depth.

The device is also equipped with an electromechanical programming system, made, for example, in the form of leading hydraulic cylinders of various diameters, the piston rods of which rest on variable-radius cams. The cams are rigidly connected to a discrete locking system, moving groups of retractable pins arranged in a specific sequence on the drum. This provides a long programmed movement of the microelectrode on the surface and in the depth of the brain of the studied animal.

To indicate the position of the microelectrode in the section under study, a light indicator panel was installed in the proposed device.

FIG. I shows the orthogonal manipulator of the proposed device; in fig. 2- its electromechanical programming

system, top view, in section; in fig. 3–9 is the same, sections in BB, B – B, G – D, D – D, E – E, G – M, and 3–3 in FIG. 2; in fig. 10- electric circuits of the programming system; in fig. 11-electrical indicator display board.

The device is an orthogonal manipulator, made in the form of three series-connected actuators, which can be hydraulic cylinders /. At the same time, each subsequent cylinder is rigidly fixed on the rod of the previous one. On the rod of the last, third, pindra microelectrode 2 is rigidly fixed, connected by conductor 5 with register.

or annoying device. The manipulator is placed above the installation for fixing the skull of the test animal.

the object under study (for example, outside the shielding chamber), which provides protection against interference.

To each of the three hoses 4 in the driving system, several driving cylinders 5 of different diameters are attached, including a 6 "deci-cylinder 6 with a piston area constituting Vio of the area of the outer slave cylinders /.

The spring-loaded pin 7 of one of the cylinders 5 or 6 is in contact with a variable radius cam 8. At the same time, each of the cylinders / has its own cam. When the cam rotates, the rod 7 moves the piston and the liquid enters or is displaced into the corresponding actuating cylinder. As a result, microelectrode 2 moves in a definite way in space: it is immersed in the brain of an experimental animal or removed from there, moving along the surface of the brain and in its depth. The scaling of the displacement is carried out by simultaneous batch switching of the cylinders 5 and 6 on all three hoses. The combination of shifts of these cylinders and cams provides the necessary set of scales.

In the proposed device, the movement of a microelectrode over the surface "deep in the brain" is carried out according to a program, which is provided by a special programming electromechanical system. The programming system contains five shafts 9, 10, 11, 12, and 13 connected by discrete gears (Maltese Crosses).

Shafts 9 and 10 are connected by a pair of shchestren 14 with one pin 15 (Fig. 3). When the shaft 9 is displaced in the direction of the arrow (Fig. 2), gear 16 is inserted with a pin of 17 (accelerated stroke). The shafts 10 And // connected by a pair of gears 18 with one pin 19 (Fig. 4). When the shaft // is displaced in the direction of the arrow (FIG. 2), the gear 20 engages with the gear 21 having four pins 19 and 22 (FIG. 4).

Shafts 10 and 12 are connected by gear 23 and idling disk 24 without pins.

When the shaft 12 is displaced in the direction of the arrow (FIG. 2), the gear 23 engages with the gear 25 with five pins 26 (FIG. 6). The shafts 12 and 13 have a constant engagement with the gears 27 and 28 (Figs. 2 and 9).

The whole system is set in motion by a permanently operating electric motor with a gearbox (not shown), the output shaft of which is connected to the shaft 9. The rotation of the shaft 9 is transmitted by the engagement system to the shaft 11, which causes the cam 8 to rotate. for example, the vertical stroke of the electrode is pressed (Fig. 2.5) and the electrode performs a vertical movement.

drum 32. The magnitude of the protruding part of the pin 31 of the program drum defines the limiting immersion of the microelectrode. The contact 30, closable 31, prepares the circuit of the solenoid 33, which is finally closed by the contact 34 when the gear 23 starts to cling to and engage it with a pair of gears 18.

Solenoid short trip

10 33 displaces the shaft 11 in the direction of the arrow (“f” r. 2), and the shaft 7 slides off the end of the cam 8 onto a disk 35 with a radius corresponding to the zero position of the rod (FIG. 5). From reverse bias

15 of the spring 36 inside the solenoid 33, the shaft is held by the rod 7 over the side surface of the cam. Simultaneously with the solenoid 33, solenoids 37 38 38 are triggered, which move the shafts 9 and 12 along the arrows (Fig. 2). From

20 of the reverse displacement shaft 9 is secured by gripping the latch 39 over the rim 40 on the shaft, and the shaft 12 is held by the latch 41.

By connecting the accelerated gear 16, unnecessary exposures are eliminated at the microelectrode pivot points, and when solenoid 33 is triggered, the cam is quickly shifted to the zero position. When this happens, the shaft engagement // is released, and it returns to the working position. Val .9

30 returns to the working position. When the slot of the side 40 of the shaft 9 is aligned with the latch 41.

At the same time, the cam 8 is moved to the next position. Pins 42 on the bristle

35–23, each pass of contact 43 closes the circuit of the solenoid 44 (the normal position of the contact 45 is closed), which delays the latch 41, and the shaft 12 returns to the initial polishing. Thus, the gear 23 and the cam sitting on the shaft 12 are turned one position, the electrode moves to the next vertical.

The transverse motor and microelectrode program is set by the side pins 46

45 software reel 32, which open contact 45; in this case, the shaft 12 continues to rotate, and the microelectrode lronesites to its initial position above the verticals. Screw surface part 47 translates

50 pins 30 to a new software program.

With full rotation of the shaft 12, the gear 28 and the cam 5, which sits on the shaft 13, are transferred to the next position. The microelectrode moves to the next section.

55 On shafts 11 and 12, contacts 48 and 49 are strengthened, which slide along collectors 50, 51, respectively. At the intersection of the connected conductors, a light bulb 52 lights up indicating the position of the microelectrode

60 on the indicator board 53.

an experiment containing a device for fixing the test animal and a microelectrode with a fixing mechanism, characterized in that, in order to automatically move the microelectrode over the surface and deep in the brain of the test animal, it has an orthogonal manipulator, made in the form of three successively connected actuators, for example, executive hydraulic cylinders, each subsequent cylinder being rigidly fixed on the piston rod of the previous cylinder, and the electrode being rigidly locked captive on stock lorshn last, third, cylinder.

2. The device according to claim 1, characterized in that, for the purpose of long programmed

moving the microelectrode over the surface and deep in the brain of the animal under study, it has a programming electromechanical system, made, for example, in the form of leading hydraulic cylinders of various diameters, the stocks of which are movably connected to variable-radius cams that are rigidly connected to the discrete engagement system moving in groups retractable pins arranged in a specific sequence on the drum.

3. The device according to claim 1, characterized in that, in order to indicate the position of the microelectrode in the test section, a light indicator board is installed in it.

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