SU183581A1 - METHOD OF MANUFACTURE / -LMZINA - Google Patents

Description

A known method for the production of I-lysia by deep expression of 1.vapi of its producers, Brevibacterium, in a medium containing sources of carbon, nitrogen, phosphorus, potassium and growth stimulants, separating bacterial cells from a culture liquid, one of the known methods, and a zeitrifugation, and the subsequent separation of lysis from the culture fluid.

As the carbon source and user 11, it is proposed to extract the molecular molecules of fatty acids in the process of catalytic oxidation of iaraffins, in acetic acid, propioic o and ore, or waste water containing these acids, before releasing the culture liquid and draw the syrup and draw the syringes with these acids. its activated carbon and calcium hydroxide, and between these treatments the culture fluid (leach.

This difference provides savings in feed and ILI products, which are used in a known manner as a nutrient medium, as well as simplify the process.

The proposed method is as follows.

keep on / about ml of sterile and environment of the following composition (in h / ,,):

Corn extract2

Glucose2

i I chloride atyro 0.25

The solution of caustic sodium is adjusted to 7.0. Flasks of the imeiiot with a circular rocking chair and in a thermostat at -; - 29 - AIA are shaken for 24 hours at 250 rpm.

The culture of the producer lizi) 1a expanded in flasks was transferred to the iyeevo fermenter equipped with a broom stalker and a bubbler to iodate 100% of the air (at 1 v. 1 rpm of pereda) in the amount of 5% of the nutrient perennial amount, pushed into it. The composition of the nutrient medium is the same. as in the flasks.

In the sowing fermenter, the producer; 1 life is spun at 29–30 ° C for 2–1 hours under sternoled conditions and non-continuous air intake. As a defoamer, it is used as a bristle fat. The grown iosevuyu material is transferred to the production fermenter in the amount of 6o / o from the volume of the sterile working nutrient medium.

The working nutrient medium is prepared on the wastewater of factories producing synthetic fatty acids by catalytic oxidation of paraffin.

iroiiiopovon and oil, which serve as a carbon source for lysine biosynthesis.

Donative nantane (in o /) is added to waste iodine;

Ammonium sulfate2

MelI

Potassium phosphate monosubstituted0, 05

Potassium phosphate dvuhzameshchenny0, 05

Corn extract2

Molasses3-4

The last two components are biostimulators. Adjust the pH of the substrate to 7.0.

In the production fermenter, fermentation was produced for 72 hours at 25–30 ° C, with continuous stirring and aeration with sternlly air (per 1 vol. 1 rpm of medium). Sperm whale oil is also used as a defoaming agent.

After the end of fermentation, the culture fluid containing lysine is rolled for 15-20 mic, and then separated, after which it is acidified with hydrochloric acid to pH 5 and treated with activated carbon, which is added in the amount of 4-5Vo, 50 -55 ° C and continuous stirring for 30 minutes. Then, the culture fluid from (| EN1) from the coal.

A solution of potassium hydroxide is added to the resulting fiber. As a result of the reaction between the residue of ammonium sulphate and the hydrate of calcium oxide, calcium sulphate is formed, which precipitates, n ammonium hydroxide.

At the same time, the residue of sucrose sucrose is precipitated, being a calcium saccharite.

Calcium sulphate and calcium sugars are filtered, and ammonium hydroxide is decomposed at the next evaporation from water and almiac. The latter is distilled.

Evaporation is carried out with iodine vacuum to form a spirituous solution / l-isnna, which is crystallized upon cooling.

In order to obtain chemically pure / -lysine, the resulting crystals dissolve it in a mi volume of distilled water and precipitate 3-4 times the volume of ethyl oyr. The precipitate of 1L1 trovyv10t and dried at 60 ° C.

PRED. M et inventions

The method of production of I-lisia by deep lining its products in a nutrient medium containing sources of carbon, nitrogen, phosphorus, potassium and growth stimulants, separating bacterial cells from the culture fluid using one of the known methods, centrifuging, followed by separation (crystallization) from the culture fluid, characterized in that, in order to save food and feed products used in the test method as a nutrient medium, as well as to simplify the process, as a source carbon sink used in the process of catalytic oxidation of narafins 1P) zloscular fatty acids, mainly acetic acid, propionic oil, or waste water containing these acids, and by discharging the / -lysin, the L1y liquid culture is purified from the BeniecsTB by sequential processing it with activated carbon and calzp hydroxide with intermediate filtration.