SU1758073A1 - Device for culturing microorganisms - Google Patents

Abstract

Use: for growing fodder yeast or bacterial cultures in microbiological production. The device contains a vertical tank with a bottom and technological pipes. Circulating shells concentrically are placed in the vessel, dividing it into zones of ascending and descending flows. Aerating devices are installed at the bottom of the upflow zones. The shells are installed from the axis alternately with a gap relative to the bottom and directly on it to create a single circulation circuit. Externally, the shell has a gap relative to the bottom and is placed with excess over the others. Between the upper ends of the outer shell and the shell container closest to the axis, a conical insert was installed to drain the culture medium into the cavity of the central shell. The shells, mounted directly on the bottom, are placed with a gap relative to the conical insert for the passage of the circulating culture medium. 2 Il.

Description

The invention relates to the microbiological industry, in particular to apparatus for growing microorganisms, and is intended for use in plants for the production of fodder dressers, protein and vitamin concentrates and other products of microbiological synthesis.

A large class of apparatus for growing microorganisms, made in the form of a cylindrical container containing circulation pipes (diffusers) located inside the container, is known.

An example of such apparatus with internal circulation pipes is a device for growing microorganisms that contains a vertical cylindrical tank, circulation tubes (diffusers) located inside the tank, and aerators located under them.

The main disadvantage of these devices is the relatively low intensity of mass transfer. To increase the solubility of oxygen in devices of this type, it is necessary to increase the hydrostatic column of the liquid, which leads to an increase in energy consumption, since it is necessary to overcome a higher layer of liquid.

Closest to the invention is an apparatus containing a vertical container, concentric shells of the same height, between which the aerators are located through one gap in such a way that the aerated zones alternate with non-aerated. During aeration, upflows occur in aerated zones, and in downstream ones, descending flows occur, forming several parallel circuits of the culture medium.

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The main disadvantage is also its low mass exchange rate.

The purpose of the invention is to increase the specific productivity through the intensification of mass transfer, as well as to reduce energy consumption.

This goal is achieved due to the fact that circulating concentric shells are installed from the tank axis alternately with a gap relative to the bottom and directly to create a single circular HH circuit, while the outer shell has a clearance relative to the bottom and is placed above the others. Between its upper end and the upper end of the shell container closest to the axis, a conical insert is installed to drain the culture medium into the cavity of the central shell, and the shell installed directly Twain on the bottom, are placed with a gap relative to the conical insert for the passage of the circulating culture medium.

Fig. 1 shows an apparatus for growing microorganisms, a slit; Fig 2 - aerator.

The apparatus for growing microorganisms contains a tank 1 with a bottom 2 and a lid 3, inside of which there are concentric circulation shells 4, 5 and b, dividing the tank 1 into zones of ascending and descending flows

Moreover, the shells 4 and 5 are installed with a gap relative to the bottom 2, and their upper ends are connected by a conical insert 7 for stekzni culture medium from the peripheral zone into the cavity of the central shell 4

The shell 5 located between the shells 4 and 6 is installed directly on the bottom 2, but with a gap relative to the cone insert 7, with the formation of a zigzag passage from the central to the peripheral zone. In the lower part of the upflow zones between the shell 4 and 5 and between the shell b and the vessel wall 1 installed aerating device 8 and 9,

Aerating devices 8 and 9 are made in the form of annular collectors 10 and 11 of rectangular cross section with aerators 12 which are built at equal distances around the circumference and are made each of conical shells 13 and 14 connected by vertices and fixed in the cutouts of the walls of the collectors 10 11.

One vis cone 14 is provided with perforations 15 to allow air bubbles to escape to aeration.

To exit the exhaust air from the upper part of the space 16 formed by the shells 4 and b and the cone insert 7 serve the pipes 17

In addition to the above, the apparatus is equipped with heat exchange devices.

for the removal of heat emitted by microorganisms and the necessary technological fittings not indicated on the drawings

The operation of the apparatus for growing microorganisms is as follows.

Before launching the device into operation, its internal cavity is washed with water and disinfected with special disinfecting solutions, passing them through special washing heads (not shown on the floor drawing).

After washing and disinfection, the apparatus is filled with the nutrient medium, the pure culture of microorganisms is inoculated and the air is turned on for aeration. Air bubbles, the exit from the openings 15 of the aerators 12 rush upward and carry the culture medium along with them.

the upward flow in the gap between the shells 4 and 5. Then, by bending the upper edge of the shell 5 and partially releasing air, the culture medium enters the gap between the shells 5 and b and goes down, and the exhaust air is blown out through the pipes 17 and further the fitting fastened in the lid 3 enters the sewage treatment plant.

The culture medium, having reached the lower edge of the shell 6, bends around it and enters the next aerated zone located between the shell b and the wall of the tank 1, where it is again subjected to aeration through

The aerating device 9 rises up and along the conical insert 7 flows into the cavity of the central shell 4, goes down and the lower edge of the shell 4 closes the circulating circuit.

Thus, due to the zigzag circulating circuit containing several aerated zones, communicated sequentially, the passage path of air bubbles and their

stays in the culture medium are extended without increasing the height of the layer of aerated culture medium, which provides a deeper utilization of oxygen without increasing energy costs and provides

thereby a higher specific removal of marketable products.

Claims (1)

The invention of the Apparatus for growing microorganisms containing vertical capacity with a bottom and technological connections, concentric shells circulating in it, dividing the capacity into zones of ascending and descending flows and installed aerating devices in the lower part of the zones of ascending flows, in order to increase the specific productivity by intensifying mass exchange as well as reducing energy consumption, the circulation shells are installed from the tank axis alternately with a gap relative to the bottom and directly on it to create A single circulation contour, while the outer shell has a gap relative to the bottom and is placed with excess over the others, with a conical insert between the upper end and upper end closest to the axis of the shell capacity and fitted with a tapered insert to drain the culture medium into the cavity of the central shell, and the shell, installed directly on the bottom, placed with a gap relative to the conical insert for the passage of the circulating culture medium. Reset from 5. of gases 1 Wit