SU1603298A1 - Method of extracting antigen from trematoda eggs - Google Patents
Method of extracting antigen from trematoda eggs Download PDFInfo
- Publication number
- SU1603298A1 SU1603298A1 SU884450201A SU4450201A SU1603298A1 SU 1603298 A1 SU1603298 A1 SU 1603298A1 SU 884450201 A SU884450201 A SU 884450201A SU 4450201 A SU4450201 A SU 4450201A SU 1603298 A1 SU1603298 A1 SU 1603298A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- eggs
- antigen
- trematoda
- opisthorch
- disintegration
- Prior art date
Links
- 235000013601 eggs Nutrition 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title claims abstract description 7
- 239000000427 antigen Substances 0.000 title claims abstract description 5
- 102000036639 antigens Human genes 0.000 title claims abstract description 5
- 108091007433 antigens Proteins 0.000 title claims abstract description 5
- 241000242541 Trematoda Species 0.000 title 1
- 238000002604 ultrasonography Methods 0.000 claims abstract description 4
- 239000002504 physiological saline solution Substances 0.000 claims abstract 2
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 241000869417 Trematodes Species 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 2
- 239000000725 suspension Substances 0.000 abstract description 2
- 108010034145 Helminth Proteins Proteins 0.000 abstract 1
- 244000000013 helminth Species 0.000 abstract 1
- FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 5
- 229960003964 deoxycholic acid Drugs 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 229940009976 deoxycholate Drugs 0.000 description 2
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
Abstract
Изобретение относитс к медицине, а именно - к паразитологии, и может быть использовано в иммунодиагностике. Способ выделени антигена из иц описторхов, заключает в дезинтеграции иц гельминтов, суспендированных в физиологическом растворе, содержащем 0,12-0,25% дезоксихолата натри , ультразвуком с помощью экспоненциального излучател при частоте 44 кГц, мощности 60-70 вт/см2 в течение 60-120 мин при концентрации иц 15-25 тыс ч в 1 мл и общем объеме суспензии 5-25 мл. Способ обеспечивает повышение выхода целевого продукта.The invention relates to medicine, namely to parasitology, and can be used in immunodiagnostics. A method of isolating an antigen from opisthorch eggs, involves the disintegration of helminth eggs suspended in physiological saline containing 0.12-0.25% deoxycholate sodium by ultrasound using an exponential radiator at a frequency of 44 kHz and a power of 60-70 W / cm 2 for 60-120 min at a concentration of eggs of 15-25 thousand hours in 1 ml and a total volume of suspension of 5-25 ml. The method provides an increase in the yield of the target product.
Description
Изобретение относитс к медицине, в частности к паразитологии, и может быть использовано в имуннодиагностике.The invention relates to medicine, in particular to parasitology, and can be used in immunodiagnostics.
Способы выделени антигена из цц описторхов не известны.Methods for isolating the antigen from the cc of opistorchs are not known.
Цель изобретени - повышение выхода целевого продукта..The purpose of the invention is to increase the yield of the target product ..
Поставленна цель достигаетс путем дезинтеграции иц описторхов. суспендированных в физиологическом растворе. рН 7,2, содержащем 0..25% дезоксихолата натри , ультразвуком с помощью экспонен- циальногго излучател при частоте 44 кГц. мощности 60-70 Вт/см а течение 60-120 мин при концентрации иц 15-25 тыс, в 1 -мл и общем обьеме суспензии 5:25 мл.The goal is achieved by disintegrating the opistorhas. suspended in saline. pH 7.2, containing 0..25% sodium deoxycholate, by ultrasound using an exponential radiator at a frequency of 44 kHz. power 60-70 W / cm and for 60-120 min at a concentration of 15-25 thousand eggs, in 1 ml and the total volume of the suspension is 5:25 ml.
Пример. Суспендируют 100000 иц в 5 мл 0.85%-ного раствора хлорида натри , рН 7.2 содержащегЬ 0.25% дезоксихолата натри , помещают в стакан прибора УЗДН-1 и подвергают дезинтеграции в рабочем оё- жимв: частота 44 кГц. мощность 60 Вт/см в течение 60 минут. Обработка иц описторхов в данном режиме обеспечивает разрушение всех иц (по данным микроскопии). После обработки взвесь охлаждают до 3 - 4° С (при этом дезоксихолат частично выпадает в осадок) И подвергают однократному центрифугированию при 10 тЫс. об./мин в течение 30 мим при 4° С (с целью удалени осколков разрушенных иц и выпавшего в осадок при охлаждении дезоксихолата). Собранную надосадочную жидкость ди.ализу- ют против 250-кратного обьема 0.85%-ного раствора хлорида натри . рН 7,2 при 4° С в течение 48 ч.Example. 100,000 eggs are suspended in 5 ml of a 0.85% sodium chloride solution, pH 7.2 containing 0.25% sodium deoxycholate, placed in the beaker of the UZDN-1 device and subjected to disintegration in operating circuits: frequency 44 kHz. power 60 W / cm for 60 minutes. Processing opisthorch eggs in this mode ensures the destruction of all eggs (according to microscopy). After treatment, the slurry is cooled to 3–4 ° C (in this case, deoxycholate partially precipitates) and subjected to a single centrifugation at 10 tons. rpm for 30 minutes at 4 ° C (in order to remove fragments of broken eggs and precipitated upon cooling of deoxycholate). The collected supernatant was dioused against a 250-fold volume of 0.85% sodium chloride solution. pH 7.2 at 4 ° C for 48 hours
Предлагаемый способ обеспечивает достоверное (0.).001) увеличение выхода белка - в 1.8 раза по:сравнению с прототипом , в 1.5 раза по сравнению с обработкой только ультразвуком, в 1.3 раза по сравнег нию с обработкой только дезоксихолатом натри ,..The proposed method provides a significant (0.). 001) increase in protein yield — 1.8 times in comparison with the prototype, 1.5 times as compared with treatment only with ultrasound, 1.3 times as compared with treatment with sodium deoxycholate, ..
Полученный предлагаемым способом антиген можно использовать дл эритроци- тарных диагностикумов.The antigen obtained by the method can be used for erythrocyte diagnostics.
О О СлЗ О О 00About About SLZ About About 00
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU884450201A SU1603298A1 (en) | 1988-06-28 | 1988-06-28 | Method of extracting antigen from trematoda eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU884450201A SU1603298A1 (en) | 1988-06-28 | 1988-06-28 | Method of extracting antigen from trematoda eggs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1603298A1 true SU1603298A1 (en) | 1990-10-30 |
Family
ID=21385409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU884450201A SU1603298A1 (en) | 1988-06-28 | 1988-06-28 | Method of extracting antigen from trematoda eggs |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1603298A1 (en) |
-
1988
- 1988-06-28 SU SU884450201A patent/SU1603298A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Hlllyev G. Pacheco Е. - Isolation and chvdrtevlratlon of Schlstosoms Haemotolluhfi . egg antigens Amer. J Trop. Med and Hud. Vol. 35. №4.777-785. 1980. * |
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