SU1602536A1 - Method of determining expendiency of vaccination against parvovirus disease of pigs - Google Patents

Abstract

The invention relates to veterinary virology and can be used in diagnostic and health measures for swine parvovirus disease. The aim of the invention is to improve the accuracy of the method. To do this, blood is collected from the piglet that has not yet taken colostrum, from which serum is separated. The serum is released from thermolabile and thermostable inhibitors, nonspecific hemagglutinins are removed, and are used to set the hemagglutination inhibition reaction. In the presence of antibodies to porcine parvovirus in the blood serum of newborn piglets not taking colostrum, in titers of 1: 8 and above, a conclusion is drawn about the active infectious process of porcine parvovirus disease and the expediency of vaccination. 2 tab.

Description

The invention relates to veterinary virology and can be used in diagnostic studies and the organization of preventive measures against porcine parvovirus disease.

 The aim of the invention is to improve the accuracy of the method,. . The method is carried out as follows. . .

A pig-head is clinically and epizootically examined, and if there are clinically impaired reproductive disorders, the sows take blood from the newly born pigs immediately after birth before their first reception of colostrum, as well as from sows. Seworp from thermolabile inhibitors is released by heating, in a water bath, from thermostable - by treatment with kaolin. To do this, 0s6 ml of 25% suspension of kaolip is added to 0.2 ml of inactivated serum. The mixture is kept at room temperature for 20 minutes, shaken occasionally, and centrifuged. Spent for 20 minutes at 2000 rpm. Cilia are sucked off and 0.1 ml of guinea pig erythrocyte sediment is added to remove non-specific gemagg-glutenins. After 1 h of contact at room temperature, erythrocytes precipitate centrifuges-316

20 min at 2000 rpm, and sera are used to formulate the gameglutination inhibition reaction (rtga). In the process, the sera are diluted 4-fold.

RTGA is placed on polystyrene plates with wells using a working dose of antigen (4 GAU) and a suspension of red guinea pig red blood cells.

The detection of antibodies to porcine parvovirus in titers of 1: 8 in serum-piglets before the intake of colostrum is evidence of an active infection process in the herd,

Example 1. Clinical, epizootologic and clinical examinations of parvovirus disease are performed on blood samples of pigs from two households (nos. 1 and 2). The results of the studies are presented in Table 1.

From the data given in Table 1, it can be seen 5 that in household No. 2 it has

the relay immunizes sows against parvovirus disease with a pig-inactivated emulsified vaccine.

In rtga, blood sera of newborn piglets are examined before and after the first intake of colostrum, obtained from vaccinated and non-immunized sows. The results of these studies are presented in table 2. ...

From the data given in Table 2, it is clear that during the period of the manifestation of the active infection process in the RTG

h

antibodies are detected in titers from 1: 8 to 1; 512 to parvovirus in the blood serum of newborns are harvested before the first dose of colostrum, and after it to 1; 1024. From the data of Table 2 it is also seen that immunization of sows with a vaccine against porcine parvovirus disease appears to be effective, since studies of blood sowed before the first intake of colostrum from vaccines

This active infectious process in parked sows does not show ana-.

swine virus disease, as in the serum of newborns, piglets, before the reception of colostrum, antibodies to pigs' parvovirus were detected. titer from 1i8 to 1; 1024. In household no. 1, an active infectious process is absent, since no newborn piglets were detected prior to the administration of colostrum to parvovirus (result i; 4 is a nonspecific reaction). The detection of antibodies to porcine parvovirus after the intake of colostrum in piglets of these households is due to the transfer of maternal colostral anti-TITL. .

The obtained data on the activity of the infectious process in pig parvovirus disease are supported by the rate of piglets per sow. They are higher (9.9) in the household I 5 where the active infectious process has not been established, and below S economic number 2 (7,, 1) where the active infectious process is established in parvo-viral disease of pigs.

Example 2B household number 3 in. the period of manifestation of clinky prizes of pig varicose veins in February and April in rtga, blood serums of 83 pigs are examined before the intake of colostrum and the same pigs after the intake of colostrum.

five

0

five

titles to parvovirus pigs.

Thus, a study in rtga blood count of newborn piglets before the first intake of colostrum allows the government to assess the situation before parvovirus. swine disease. Detection of antibodies to parvovirus. pigs before colostrum sucking in a titer of 1 g 8 and higher is evidence of their intra uterine infection with parvovirus and the presence of an actively occurring infectious process of pig parvovirus disease in this herd and indicates the need for appropriate health measures, t. e. expediency of vaccination against swine narvovirus disease, the formula of the invention

The method of determining the appropriateness of vaccination against parvovirus disease of pigs, including - taking blood from animals, getting short, and determining the level of inhibiting hemagglutination of antibodies, antibodies, and so on. that, in order to improve the accuracy of S, blood is taken from newborn piglets before the first dose of colostrum, and vaccination is considered to be -. forest-like, starting with an anti-tel level of 1: 8.

Claims (1)

Claim A method for determining the feasibility of vaccination against parvovirus disease of pigs, including blood sampling from animals, obtaining serum, determination of the level of inhibitory hemagglutination antibodies in it, about l, and especially for the purpose of increasing accuracy, blood is taken from newborn piglets before the first dose. colostrum, and vaccination is considered ce ~. woody, starting from the level of anti-bodies 1: 8. Household 5 -J _______ Number of samples tested 1602536 6 \ Table 1 ή a 1 -----; ---------- Characteristics of the group of piglets The titer of antibodies in rtga Assessment of the activity of the infectious process The output of piglets per 1 sow 1 37 Before taking colostrum 0,0-1: 4 Inactive 9.9 32 After colostrum 1:64 to 1: 1024 The result of the transmission of maternal antibodies 2 66 Before taking colostrum 1: 8 to ' 1: 1024 Active 7.1 47 After colostrum 1:64 to 1: 4096 The result of additional transmission of maternal antibodies with colostrum .T_a_b_l_i_ts [_a__2 .1 ____ Groups of piglets Quantity Characteristic Antibody titer Activity rating Exit in time groups of piglets in rtga infectious piglets Syat cessa on 1 * nomad Newborns received from 83 Before taking mo. the vine From 1: 8 to 1: 512. Active 8.1 non-immunized sows 82 After colostrum 1: 256 to 1: 1024 The result of additional transmission of antibodies with colostrum Newborn Piglets 81 Before taking colostrum 0,0-1: 4 Inactive 9.7 vaccinated sows 80 After taking my 1: 128 to Transfer result the vine 1: 51 2 maternal antibodies with youth