SU1683583A1 - Method for microclonal reproduction of plants - Google Patents
Method for microclonal reproduction of plants Download PDFInfo
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- SU1683583A1 SU1683583A1 SU894686309A SU4686309A SU1683583A1 SU 1683583 A1 SU1683583 A1 SU 1683583A1 SU 894686309 A SU894686309 A SU 894686309A SU 4686309 A SU4686309 A SU 4686309A SU 1683583 A1 SU1683583 A1 SU 1683583A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- plants
- growth
- sterile conditions
- micro
- vitro
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 5
- 235000015097 nutrients Nutrition 0.000 claims abstract description 6
- 238000000338 in vitro Methods 0.000 claims abstract description 5
- 230000005486 microgravity Effects 0.000 claims 1
- 238000002054 transplantation Methods 0.000 claims 1
- 238000005520 cutting process Methods 0.000 abstract description 3
- 238000010367 cloning Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 4
- 239000003630 growth substance Substances 0.000 description 4
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- YUXPJMWDDQCARU-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanol;2-(2-methylphenoxy)acetic acid Chemical compound OCCN(CCO)CCO.CC1=CC=CC=C1OCC(O)=O YUXPJMWDDQCARU-UHFFFAOYSA-N 0.000 description 1
- METVSRFIOHSNJX-UHFFFAOYSA-N 5-(chloromethyl)-4,6,11-trioxa-1-aza-5-silabicyclo[3.3.3]undecane Chemical compound O1CCN2CCO[Si]1(CCl)OCC2 METVSRFIOHSNJX-UHFFFAOYSA-N 0.000 description 1
- 241000219068 Actinidia Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N inositol Chemical compound OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Изобретение относитс к биотехнологии , в частности к способам микроклонзль- ного размножени растений. Целью изобретени вл етс повышение укорен емости растений, усиление роста побегов и корневой системы In vitro и в нестерильных услови х. Микроклональное размножение растений провод т путем микрочеренковани растений - регенератов, посадки микро- черенков на питательную среду Мурасиге-Скуге, в которую дополнительно ввод т 0,1-5,0 мг/л крезацина. Способ позвол ет увеличить укорен емость микрочеренков In vitro, улучшаетс рост побега и корневой системы в культуре ткани и в нестерильных услови х. 3 табл.The invention relates to biotechnology, in particular to methods of micro-cloning propagation of plants. The aim of the invention is to increase plant rootedness, enhance shoot growth and root system in vitro and in non-sterile conditions. Microclonal propagation of plants is carried out by micro-cutting of regenerated plants, planting micro-cuttings on the Murashige-Skoge nutrient medium, into which 0.1-5.0 mg / l of krezacin is additionally introduced. The method allows to increase the rooting of microchips in vitro, improves the growth of the shoot and root system in tissue culture and in non-sterile conditions. 3 tab.
Description
слcl
сwith
Изобретение относитс к биотехнологии , в частности к способам микроклональ- ного размножени растений.The invention relates to biotechnology, in particular to methods of microclonal propagation of plants.
Целью изобретени вл етс повышение укорен емости растений, усиление роста побегов и корневой системы In vitro и в нестерильных услови х.The aim of the invention is to increase plant rootedness, enhance shoot growth and root system in vitro and in non-sterile conditions.
Пример осуществлени . Микроклональное размножение провод т на культуре земл ники, груши, актинидии . Провод т микрочеренкование растений-регенерантов и побеги высаживают на питательную среду Мурасиге-Скуге следующего состава, мг/л:An example of implementation. Microclonal reproduction is carried out on a culture of earthlings, pears, and actinidia. Microcooling of regenerated plants is carried out and shoots are planted on a Murashige-Sogge nutrient medium of the following composition, mg / l:
Азотнокислый алюминий1650Aluminum Nitrate 1650
Азотнокислый калий1900Potassium Nitrate1900
Хлористый кальций440Calcium chloride440
Сернокислый магний370Magnesium sulphate370
Калий однозамещенный фосфорнокислый170Potassium monobasic phosphate170
Натрий ЭДТА37,3Sodium EDTA37,3
Сернокислое железо27,8Iron sulfate27.8
Борна кислота6,2Borna acid6,2
Хлористый кобальт0,025Cobalt chloride0,025
Сернокисла медьCopper sulfate
СернокислыйSulphate
марганецmanganese
Натрий молибденовокислыиSodium molybdate
Йодистый калийPotassium iodide
Сернокислый цинкZinc Sulphate
ТиаминThiamine
ПиридоксинPyridoxine
МезоинозитMesoinosite
СахарозаSucrose
ArapvArapv
Бидистиллированна Double distilled
водаwater
В качестве регул торов роста, стимулирующих корнеобразование используют ИМКв концентрации 0,2-2 мг/л. В качествг регул торов роста, стимулирующих корнIMCv concentrations of 0.2–2 mg / l are used as growth regulators stimulating rooting. As a growth regulator, stimulating root
до 1 лup to 1 l
образование, рост надземной системы In vitro и в нестерильных услови х к питательной среде, представленной выше, добавл ют мивал, крезацин.formation, growth of the above-ground system In vitro and under non-sterile conditions, mival, cresacin, is added to the nutrient medium presented above.
Все опыты выполн ют в трехкратной повторности по 10 пробирок в повторности.All experiments were performed in triplicate with 10 test tubes per replicate.
На культуре земл ники сорта Фестивальна вли ние концентрации дополнительно введенного в ср еду регул тора роста крезацина показано в табл. 1,In cultured varieties of the variety “Festival”, the effect of the concentration of the addition of the growth regulator krezacin is shown in Table. one,
Как видно из таблицы, введение в состав среды крезацина совместно с индукторами корнеобразовани в количестве 0,1-5,0 мг/л приводит к увеличению процента укорененных мйкрочеренков до 99- 100%, повышаетс количество корней и их длинаAs can be seen from the table, the introduction of krezacin together with root inducers in an amount of 0.1-5.0 mg / l leads to an increase in the percentage of rooted mycrostocees up to 99-100%, the number of roots and their length increase
Положительное воздействие введени в среду крезацина про вл етс также на росте побегов земл ники (см. табл, 2).The positive effect of the introduction of krezacin into the medium is also manifested in the growth of shoots of earthlings (see Table 2).
Из таблицы видно, что дополнительное введение в среду крезацина повышает рост и развитие побега.The table shows that the additional introduction of krezacin into the medium increases the growth and development of the shoot.
Последействие регул тора сохран етс при пересадке укорененных микрочеренков в нестерильные услови (см табл. 3).The post-action of the regulator is preserved when the rooted micrografts are transplanted into non-sterile conditions (see Table 3).
Вли ние регул торов роста на рост укорененных черенков земл ники в нестерильных услови х (через 2 недели) показано в табл. 3.The effect of growth regulators on the growth of rooted cuttings of earthlings under non-sterile conditions (after 2 weeks) is shown in Table. 3
Таким образом, введение крезацина в состав питательной среды, содержащей индуктор корнеобразовани крезацмна, увеличивает укорен емость микрочеренков, повышает рост побега и корневой системы. При этом стимулирующий эффект про вл етс при пересадке растений в нестерильные услови .Thus, the introduction of krezacin into the composition of the nutrient medium containing the kresatzna root formation inducer increases the rootedness of the microcultures, increases the growth of the shoot and the root system. However, the stimulating effect is manifested when the plants are transplanted into non-sterile conditions.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU894686309A SU1683583A1 (en) | 1989-03-28 | 1989-03-28 | Method for microclonal reproduction of plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU894686309A SU1683583A1 (en) | 1989-03-28 | 1989-03-28 | Method for microclonal reproduction of plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1683583A1 true SU1683583A1 (en) | 1991-10-15 |
Family
ID=21445243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU894686309A SU1683583A1 (en) | 1989-03-28 | 1989-03-28 | Method for microclonal reproduction of plants |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1683583A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2128430C1 (en) * | 1996-08-09 | 1999-04-10 | Горский государственный аграрный университет | Nutrient medium for microclonal reproduction of cherries |
| RU2440414C1 (en) * | 2010-05-27 | 2012-01-20 | Денис Геннадьевич Шорников | Nutrient medium for microreproduction of chinese magnolia (schisandra chinensis (turcz) baill) in conditions in vitro |
-
1989
- 1989-03-28 SU SU894686309A patent/SU1683583A1/en active
Non-Patent Citations (1)
| Title |
|---|
| P.Boxus. etal., In vitro culture of srtanoterry plants, 1987, p. 50. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2128430C1 (en) * | 1996-08-09 | 1999-04-10 | Горский государственный аграрный университет | Nutrient medium for microclonal reproduction of cherries |
| RU2440414C1 (en) * | 2010-05-27 | 2012-01-20 | Денис Геннадьевич Шорников | Nutrient medium for microreproduction of chinese magnolia (schisandra chinensis (turcz) baill) in conditions in vitro |
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