SU1576558A1 - Device for isolating campylobacteria from sample - Google Patents

Abstract

This invention relates to laboratory devices for isolating campylobacter in its pure form in the diagnosis of campylobacteriosis. The purpose of the invention is to increase the completeness of secretion and to create conditions for the fractional separation of campylobacteria from the accompanying microflora. The device contains a filter element 1, the housing 2 in the form of a ring. The filter element 1 consists of a sebic or track membrane with a pore diameter of 0.4-0.7 µm, placed on one of the end surfaces of the housing 2, attached to its outer surface by a pressure ring 5. The device is used as part of a Petri dish 6 with nutrient 7. Test sample 8 is placed on the surface of the membrane, facing the inside of housing 2. The device improves the accuracy of diagnosis of campylobacteriosis and eliminates contamination of the nutrient medium by accompanying microflora. 2 Il.

Description

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The invention relates to medical microbiology, to laboratory methods for diagnosing campylobacteriosis, to devices for isolating campylobacter cells in their pure form from a sample.

The aim of the invention is to increase the completeness of the release and to create conditions for the fractional separation of campylobacter from the accompanying microflora.

FIG. 1 shows a device for isolating campylobacter, a longitudinal section, the gaps between the elements are enlarged :, in FIG. 2 - device as part of a Petri dish with a nutrient medium, a longitudinal section.

A device for isolating campylobacter from a sample contains a filter element 1, a housing 2 in the form of a ring. The filter element 1 consists of a membrane or track membrane with a POR diameter of 0.4–0.7 µm placed on one of the end surfaces 3 of the housing 2 and attached to its outer surface 4 by means of a wear ring 5.

The device is placed in a Petri dish 6 on the surface of a solid nutrient medium 7, and the membrane surface of the filter element 1 facing the inside of the body 2 serves to place the sample 8.

The device works as follows.

Before starting the operation, the device is assembled, the nuclear or track membrane with a pore diameter of 0.4-0.7 μm is placed on one of the end surfaces 3 of the housing 2 and pressed against the lateral surface 4 of the housing 2 with a pressure ring 5, the inner diameter of which is equal to the outer diameter of the housing 2, while the membrane area should be 1.5–2 times larger than the end surface of the housing 3

The device is then assembled and sterilized and placed in a Petri dish 6 on the surface of solid nutrient medium 7 with the membrane down, and the test sample 8 is placed on the membrane. After 15-30 minutes, the device is removed from the Petri dish, washed, sterilized and reused.

During this time, cachobacteria pass through the pores of the membrane and remain on the surface of the nutrient medium in the Petri dish. After removing the device, the selected cams

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Pilobacteria are further analyzed. The process of shitowaning, fractionation, carried out with the help of nuclear (track) membranes, is aimed at the passage through the membrane of particles having a size less than a given 0.4-0.7 microns. In addition, the direct short pores of the nuclear membrane facilitate the chemotaxis of campylobacter.

The pore size of the membrane is selected taking into account the size of campylobacter and the accompanying microflora. A membrane with pores with a diameter of less than 0.4 μm detains Campylobacter, and a membrane with a pore diameter of more than 0.7 μm does not detain the accompanying microflora.

As a result of the application of the proposed device, the effectiveness of this method is doubled, and due to the better delay of extraneous microflora in some cases, it may exceed the sensitivity of the method using antibiotics.

Fixing the membrane in the housing 2 eliminates contamination of the nutrient medium during the application of the material and the removal of the device, allows you to increase the material load per unit area of the membrane, provides for reuse of the membrane, simplifies planting technology. Due to the absence of the inhibitory effect of antibiotics in 50% of cases, a positive result (campylobacter secretion) can be obtained after 24 hours, i.e. the response time is halved.

Thus, the proposed device makes it possible to isolate campylobacteria from the sample in its pure form, to separate the accompanying microflora and to culture only campylobacteria on the nutrient medium, which improves the accuracy and reliability of analyzes in the diagnosis of campylobacteriosis.

Claims (1)

Invention Formula A device for isolating campylobacteria from a sample containing a filtering element, characterized in that, in order to increase the completeness of the selection and create conditions for the fractional separation of campylobacteria from the accompanying microflora, the device is provided with a ring-shaped body, with the filtering element placed on one of the end surfaces51576558 The body and attached to its outermost part is made up of the surface or its surface with the help of a pressure-tracking membrane with a pore ring diameter, with a filter element of 0.4-0.7 µm. / Ј & city / V