SU1576063A1 - Method of obtaining salt resistant forms of sunflower - Google Patents

Abstract

The invention relates to breeding and can be used to obtain salt-resistant forms. The purpose of the invention is to increase the number of salt-resistant forms. Seeds are kept in water for 16-20 hours. Then, no later than after 6 hours, seeds are treated with a solution of N-nitrosomethylurea with a concentration of 0.008-0.12%. The treatment is carried out for 6-12 hours at 20-25 ° C. After the treatment, the seeds are sown and salt resistant forms are isolated in the progeny. 4 tab.

Description

The invention relates to breeding and can be used to obtain salt-tolerant forms with high efficiency.

The purpose of the invention is to increase the number of salt-resistant forms.

The method is carried out as follows.

Sunflower seeds are kept in water without air access in a volume exceeding the volume of seeds 9-10 times (anaerobic conditions) for 16-20 hours. Then they are germinated for 6-12 hours in a solution of N-nitrosomethylurea (NIM) concentration 0.008-0.012% not later than 6 hours after being kept in water. Germination is carried out at 20-25 ° C.

As a result of keeping the seeds in water (anaerobic conditions), there is a simple swelling of the seeds, which precedes the start of growth processes in the seed. One of these

processes is the replication of plasmid DNA. The soaking procedure synchronizes the onset of germination in all seeds, and therefore ensures the simultaneous achievement of the onset of plastid DNA synthesis. The subsequent germination of seeds after soaking in mutagen solutions for the first 6 hours causes the mutagen to be effective at the time of plastid DNA replication, as the DNA sodder begins to replicate later.

Example Sunflower seeds of the inbred line 3629 are kept in water for 12, 16, 20 and 24 hours. Then, 100 wetted seeds from each variant are germinated in Petri dishes in HMM aqueous solutions in concentrations,%: 0.005; 0.008; 05012 and 0,015 at temperatures of 15; 20; 25 and 30 ° C for 3, 6, 9 and 12 hours, as well as within 6 hours later

sl 1

35

About 05 WITH

6 and 12 hours after soaking. After germination in a mutagen solution, seeds are sown in the field and plants M are obtained (0 In the progeny of plants Mi, which is called M2, individuals with increased salt tolerance are found. About the frequency of salt-resistant forms in relation to the plants that have sprung up at 1.7% chloride salinity to the total number of planted seeds. At least 50 families of M. and at least 500 seeds of M2 are analyzed.

In tab. Figure 1 shows the effect of the soaking time of the seeds and the concentration of the mutagen on the frequency of salt-resistant forms (processing time 6 hours immediately after soaking at 25 ° C).

 In tab. Figure 2 shows the effect of treatment temperature on the frequency of salt-resistant forms (soaking for 16 hours, germination for 6 hours immediately after the end of soaking in 0.01% HMM gravel).

In tab. Figure 3 shows the effect of the time of action of the mutagen (LMM concentration of 0.01% immediately after soaking) on the frequency of salt-tolerant forms (soaking for 16 hours, treatment temperature 25 ° C).

five

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five

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Table 4 shows the effect of the time interval between the end of soaking and the onset of the action of the mutagen on the induction of salt-tolerant forms (soaking for 16 hours, HMM concentration 0.01%, HMM action time 6 hours, treatment temperature 25PC).

The proposed method makes it possible to increase the level of salt-tolerant forms in the sunflower population by almost 4 times compared with the natural one.

Claims (1)

Invention Formula The method of obtaining salt-tolerant forms of sunflower, including seed treatment with a solution of N-nitrosomethylurea, their subsequent seeding and isolation of salt-tolerant forms in the progeny, characterized in that, in order to increase the number of salt-tolerant forms, the seeds are kept in water for 16-20 before treatment h, seed treatment with a solution of N-nitrosomethylurea is carried out no later than 6 h after keeping the seeds in water, and the treatment is carried out with a 0.008-0.022% solution of mutagen for 6-12 h at 20-25 ° C. HMM concentration,% Frequency of salt tolerance,%, during soaking time. h 12 I Table 2 Table 1 R 20 g 24 50 Taiza The time interval (germination on water) between the end of soaking and the beginning of the action, HMM, h O 6 12 Compiled by E.Shkradyuk Editor A. Kozoriz Tehred M. Didyk Proofreader V. Kabatsy Order 1805 Circulation 439 VNIIPI State Committee for Inventions and Discoveries at the State Committee on Science and Technology of the USSR 113035, Moscow, Zh-35, Raushsk nab. 4/5 Production and publishing plant Patent, Uzhgorod, st. Gagarin, 101 Salinity stability frequency,% 24.6 ± 1.9 12.4 ± t.5, 4.5 ± 0.23t Subscription