SU1544438A1 - Method of plasmosorption - Google Patents

Abstract

This invention relates to medicine, in particular to hematology. The method is carried out as follows: the blood is expelled sequentially into the Gemakon 500/300 containers. Erythrocytes are precipitated by centrifugation, the isolated plasma is mixed with three times washed donor erythrocytes of long shelf life. Autoerythrocytes are returned to the patient. The erythrocytes of the donor are precipitated by centrifugation and removed. The purified plasma is transferred to the receiving container and returned to the patient. During the plasma sorption session, 2.0-2.5 liters of plasma are purified.

Description

The invention relates to medicine, namely to hematology.

The aim of the invention is to reduce the morbidity of the method.

The method is carried out as follows.

Washed donor erythrocytes are harvested under conditions of blood transfusion by 3 times washing in an isotonic solution of 0.9% sodium chloride according to the standard procedure. Then, by puncture of the ulnar vein, 500 ml of blood is sequentially expelled into the plastic containers Gemakon 500/300 with hemoconservative. The blood is centrifuged, divided into plasma and shaped elements (erythrocytes). Autoerythrocytes are returned to the patient immediately after each exposure. Patient's plasma is mixed in equal proportions (1: 1) with washed donor erythrocytes for 20-21 days of storage, centrifuged to precipitate erythrocytes of the donor and remove them after cleaning each portion of plasma. The supernatant layer of purified plasma is collected in a receiving container and returned to the patient's body in the TV of the veins of the same drip.

During the plasma sorption session 2 2.5 liters of plasma are cleaned.

Example. Patient S., 15 years old, and / b No. 1153, diagnosed with acquired autoimmune hemolytic anemia. He entered the hematological clinic of the Leningrad Scientific Research Institute of Hematology and Blood Transfusion on October 6, 1983. The patient was scheduled to undergo splenectomy, but suddenly there was a sharp deterioration in the patient's condition: the skin and sclera intensified, dark urine, weakness, nausea, vomiting appeared. For the purpose of preoperative preparation, an intensive infusion therapy was carried out. Due to the failure of the last 20 October and 21 October, extracorporeal hemosorption was performed twice, but no clear effect was obtained. On October 24, 1983, it was decided to perform plasma adsorption using washed single-group donor erythrocytes: during the procedure 2.5 liters of plasma were cleaned and returned to the patient. As a result of the operation, an improvement in the patient's condition was noted, nausea and vomiting stopped, and laboratory parameters improved. Plasmasorption was performed using plastic containers “Gemakon 500.

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Puncture of the ulnar zone was performed by sequentially exchanging blood into containers in a volume of 500 ml. Produced 7 exfusions. At the K-70 centrifuge at 4 ° C in 2000 rpm mode, blood was divided into plasma and shaped elements for 5 minutes. The latter, as they were released, returned to the patient’s body intravenously, the plasma was connected to the washed red blood cells and, after centrifugation to remove the used red blood cells, was also returned to the patient. The patient underwent plasmasorption satisfactorily, there were no complications. The patient's condition improved so much that it was possible to perform a splenectomy. The postoperative period was uneventful, pain0

Noah was discharged for outpatient treatment in a satisfactory condition.

Claims (1)

The proposed method of plasma purification using washed donor erythrocytes improves plasma purification by eliminating the dust content of the sorbent and its aggressiveness with respect to plasma proteins, reducing the plasma protein absorption level, and reducing plasma urea. Invention Formula A plasma sorption method by passing blood plasma through a hemosorbent, characterized in that, in order to reduce the morbidity of the method, washed donor red blood cells are used as a hemosorbent.