SU1341566A1 - Solion for determining concentration of glucose - Google Patents

Abstract

The invention relates to a technique for analyzing and analyzing materials by determining the electrochemical and chemical parameters and can be used to determine the concentration of glucose in biological solutions, for example, in blood. The purpose of the invention is to expand the range of measured concentrations by increasing the linearity of the calibration graph. For this, a diffusion membrane between the microporous and enzyme membranes is additionally introduced. As a diffusion membrane, cellulose acetate is used with an acetylation degree of 1.9-2.8, partially quaternized polyvinylpyridine or polyhydroquinone 18-25 microns thick. 1 hp f-ly 1 silt, 1 tab. about s (L with 4 sd with: 05

Description

one

The invention relates to a technique for researching and analyzing materials by determining electrochemical parameters, namely, electrochemical sensors for determining the concentration of glucose in biological solutions, for example, in blood.

The aim of the invention is to expand

the broadening of the range of variable glucose concentrations in the physiological solution (temperature 22 ° C) on the drawing shows a diagram of the membranes of the proposed electrochemical sensor for determining glucose

The electrochemical sensor consists of a platinum anode 1 with a separation membrane 2 attached to it, equipped with an enzyme 3 and microporous 4 membranes, and a diffusion membrane 5 is additionally superimposed between them, which is a cellulose acetate membrane with an acetylation degree of 1.9-2.8. The membranes are made of partially quantified polyvinylpyridine or polyhydroquinone, whose thickness is 18-25 microns.

The device works as follows.

On the platinum anode impose a separation membrane, which is 5 microns thick. A 5 µm thick enzyme membrane, a cellulose acetal cellulose diffusion membrane of 2.8 µm thick and 25 µm thick, and a microporous membrane are applied to the separation membrane. 1-2 drops of a physiological solution are applied to the membranes and residual current is determined. The solution is dried with filter paper and 1-2 drops of glucose are applied in a physiological solution. The stationary current of the electrodes, which is established in 1-1.5 min, is determined by

The proposed O 3 7 10 14 21 34 60 10 136 172 206 290

t

The known About 105 210 318 345 350 353 360 354 - - - 15662

using the flooring LP-7e. After measurement, the sensor is washed with 1-2 drops of saline and measurements are made with a solution of other glucose concentrations.

The current dependence of the proposed sensor and known, i.e. when there is no diffusion membrane, from

listed in the table

From the data in the table it follows that the sensor predicted has a linear relationship between current and glucose concentration up to 30 mM, whereas the calibration graph of the known sensor bends even at a concentration of 1.5 mM.

The sensor remains operable for at least 20 days at 20-24 ° C and periodic calibration.

Claims (2)

1. Electrochemical sensor for determining the glucose concentration containing a platinum anode, at the end of which there are adjoining separating, enzymatic and microporous membranes, characterized in that, to expand the range of measured concentrations, a diffusion membrane is installed between the microporous and enzyme membranes with a diffusion coefficient for glucose 2,, 0-10. 2. A POP.1 sensor, characterized in that a diffusion membrane is used cellulose acetate with an acetylation degree of 1.9-2.8, polyvinylpyridine with a quaternization degree of 0.23-0.31, or polyhydroquinone 18-25 microns thick. / g 5 h Editor E. Sligan Compiled by I. Rogal Tehred M. Didyk Order 4431/49 Circulation 776 Subscription VNIIPI USSR State Committee for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5 Production and printing company, Uzhgorod, st. Project, 4 Proofreader A.T. sko