SI2379734T1 - Method for controlling streptococcus pneumoniae serotype 19a polysaccharide molecular weight - Google Patents
Method for controlling streptococcus pneumoniae serotype 19a polysaccharide molecular weight Download PDFInfo
- Publication number
- SI2379734T1 SI2379734T1 SI200931830T SI200931830T SI2379734T1 SI 2379734 T1 SI2379734 T1 SI 2379734T1 SI 200931830 T SI200931830 T SI 200931830T SI 200931830 T SI200931830 T SI 200931830T SI 2379734 T1 SI2379734 T1 SI 2379734T1
- Authority
- SI
- Slovenia
- Prior art keywords
- fermentation culture
- streptococcus pneumoniae
- addition
- molecular weight
- feeding
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 17
- 150000004676 glycans Chemical class 0.000 title claims abstract 8
- 229920001282 polysaccharide Polymers 0.000 title claims abstract 8
- 239000005017 polysaccharide Substances 0.000 title claims abstract 8
- 241000193998 Streptococcus pneumoniae Species 0.000 title claims abstract 7
- 229940031000 streptococcus pneumoniae Drugs 0.000 title claims abstract 7
- 238000000855 fermentation Methods 0.000 claims abstract 24
- 230000004151 fermentation Effects 0.000 claims abstract 24
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims abstract 4
- 230000001580 bacterial effect Effects 0.000 claims abstract 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims abstract 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 2
- 239000002775 capsule Substances 0.000 claims 1
- 230000009089 cytolysis Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 235000017557 sodium bicarbonate Nutrition 0.000 claims 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims 1
- 235000017550 sodium carbonate Nutrition 0.000 claims 1
- 229910000029 sodium carbonate Inorganic materials 0.000 claims 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Tropical Medicine & Parasitology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The present invention provides improved methods for producing a solution containing high molecular weight isolated Streptococcus pneumoniae 19A capsular polysaccharides. In certain methods, a fermentation culture of Streptococcus pneumoniae bacterial cells that produce serotype 19A capsular polysaccharides is fermented for less than 6 hours before the bacterial cells are lysed the capsular polysaccharides are harvested. In other methods, CO2 is supplied to the fermentation culture. Supplying CO2 to the fermentation culture includes adding bicarbonate ions to the fermentation culture, adding carbonate ions to the fermentation culture, adding mixtures of bicarbonate and carbonate ions to the fermentation culture, and overlaying the fermentation culture with CO2.
Description
Original document published without description
Claims (11)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13856308P | 2008-12-18 | 2008-12-18 | |
| EP09795862.3A EP2379734B1 (en) | 2008-12-18 | 2009-12-17 | Method for controlling streptococcus pneumoniae serotype 19a polysaccharide molecular weight |
| PCT/US2009/068429 WO2010080486A2 (en) | 2008-12-18 | 2009-12-17 | Method for controlling streptococcus pneumoniae serotype 19a polysaccharide molecular weight |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SI2379734T1 true SI2379734T1 (en) | 2018-05-31 |
Family
ID=42266457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SI200931830T SI2379734T1 (en) | 2008-12-18 | 2009-12-17 | Method for controlling streptococcus pneumoniae serotype 19a polysaccharide molecular weight |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US8795689B2 (en) |
| EP (1) | EP2379734B1 (en) |
| JP (1) | JP5843612B2 (en) |
| KR (2) | KR101593770B1 (en) |
| CN (1) | CN102257155B (en) |
| AU (1) | AU2009335826B2 (en) |
| BR (1) | BRPI0922981A8 (en) |
| CA (1) | CA2743710C (en) |
| DK (1) | DK2379734T3 (en) |
| ES (1) | ES2666698T3 (en) |
| HU (1) | HUE037465T2 (en) |
| IL (1) | IL213169A (en) |
| MX (1) | MX2011006432A (en) |
| PL (1) | PL2379734T3 (en) |
| PT (1) | PT2379734T (en) |
| RU (1) | RU2511404C2 (en) |
| SI (1) | SI2379734T1 (en) |
| WO (1) | WO2010080486A2 (en) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101307703B1 (en) * | 2008-12-18 | 2013-09-11 | 와이어쓰 엘엘씨 | Method for controlling streptococcus pneumoniae polysaccharide molecular weight using carbon |
| KR101450958B1 (en) | 2009-04-30 | 2014-10-15 | 콜레이 파마시티컬 그룹, 인코포레이티드 | Pneumococcal vaccine and uses therof |
| BR112014014656B1 (en) * | 2011-12-15 | 2020-03-17 | Serum Institute Of India Ltd. | BACTERIA CULTIVATION METHOD FOR SUPERIOR VOLUMETRIC PRODUCTION OF CAPSULARY POLIOES IN COMPARISON WITH Batch Fermentation |
| BR112015005081B1 (en) * | 2012-09-07 | 2021-10-26 | Sk Bioscience Co., Ltd. | METHOD FOR PRODUCING A CAPSULAR POLYSACCHARIDE HAVING A PNEUMOCOCCAL SEROTYPE |
| KR20190103256A (en) | 2016-12-30 | 2019-09-04 | 수트로박스, 인코포레이티드 | Polypeptide-antigen conjugates with unnatural amino acids |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| WO2018144439A1 (en) | 2017-01-31 | 2018-08-09 | Merck Sharp & Dohme Corp. | Methods for making polysaccharide-protein conjugates |
| BR112019017560A2 (en) | 2017-02-24 | 2020-04-07 | Merck Sharp & Dohme | enhancement of immunogenicity of streptococcus pneumoniae polysaccharide-protein conjugates |
| CN111132691A (en) | 2017-06-10 | 2020-05-08 | 创赏有限公司 | Multivalent conjugate vaccines with bivalent or multivalent conjugate polysaccharides providing improved immunogenicity and avidity |
| US10729763B2 (en) | 2017-06-10 | 2020-08-04 | Inventprise, Llc | Mixtures of polysaccharide-protein pegylated compounds |
| CA3074703A1 (en) | 2017-09-07 | 2019-03-14 | Merck Sharp & Dohme Corp. | Pneumococcal polysaccharides and their use in immunogenic polysaccharide-carrier protein conjugates |
| US11389540B2 (en) * | 2017-09-07 | 2022-07-19 | Merck Sharp & Dohme Llc | Pneumococcal polysaccharides and their use in immunogenic polysaccharide-carrier protein conjugates |
| MX2020005779A (en) | 2017-12-06 | 2020-10-28 | Merck Sharp & Dohme | COMPOSITIONS COMPRISING CONJUGATES OF STREPTOCOCCUS PNEUMONIAE POLYSACCHARIDE WITH PROTEIN AND METHODS OF USE THEREOF. |
| TW202011985A (en) * | 2018-04-18 | 2020-04-01 | 韓商Ｓｋ生物科學股份有限公司 | Capsular polysaccharide of Streptococcus pneumoniae and its immunogenic adapter |
| KR20210005158A (en) | 2018-04-30 | 2021-01-13 | 머크 샤프 앤드 돔 코포레이션 | Method for producing a capsular polysaccharide carrier protein conjugate to Streptococcus pneumoniae |
| KR102827174B1 (en) | 2018-04-30 | 2025-06-27 | 머크 샤프 앤드 돔 엘엘씨 | Method for providing a homogeneous solution of lyophilized mutant diphtheria toxin in dimethyl sulfoxide |
| JP7489324B2 (en) | 2018-04-30 | 2024-05-23 | メルク・シャープ・アンド・ドーム・エルエルシー | Method for producing Streptococcus pneumoniae capsular polysaccharide carrier protein conjugates from riospheres - Patent Application 20070233333 |
| KR20240169145A (en) | 2018-12-19 | 2024-12-02 | 머크 샤프 앤드 돔 엘엘씨 | Compositions comprising streptococcus pneumoniae polysaccharide-protein conjugates and methods of use thereof |
| EP4192499A4 (en) | 2020-08-10 | 2024-09-25 | Inventprise, Inc. | MULTIVALENT PNEUMOCOCCAL GLYCOCONJUGATE VACCINES CONTAINING AN EMERGING 24F SEROTYPE |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1115210A (en) | 1977-11-28 | 1981-12-29 | Dennis J. Carlo | Pneumococcal vaccine |
| US4221906A (en) * | 1979-03-19 | 1980-09-09 | American Cyanamid Company | Stabilization of pneumococcal polysaccharides |
| US4242501A (en) | 1979-08-08 | 1980-12-30 | American Cyanamid Company | Purification of pneumococcal capsular polysaccharides |
| FR2495939B1 (en) | 1980-12-11 | 1985-10-11 | Merieux Inst | PROCESS FOR THE PURIFICATION OF STREPTOCOCCUS PNEUMONIAE POLYOSIDES AND VACCINE BASED ON THE PURIFIED POLYOSIDES |
| BE889979A (en) | 1981-08-14 | 1982-02-15 | Smith Kline Rit | PROCESS FOR THE PREPARATION OF PURIFIED ANTIGENIC CAPSULAR BACTERIAL POLYSACCHARIDES, PRODUCTS OBTAINED AND THEIR USE |
| US4686102A (en) * | 1984-04-12 | 1987-08-11 | American Cyanamid Company | Multivalent pneumococcal vaccine and preparation thereof |
| CA2059693C (en) * | 1991-01-28 | 2003-08-19 | Peter J. Kniskern | Polysaccharide antigens from streptococcus pneumoniae |
| CA2059692C (en) * | 1991-01-28 | 2004-11-16 | Peter J. Kniskern | Pneumoccoccal polysaccharide conjugate vaccine |
| US5314822A (en) * | 1992-10-15 | 1994-05-24 | Merck & Co., Inc. | Method of clonal growth of Streptococcus pneumoniae |
| US5714354A (en) * | 1995-06-06 | 1998-02-03 | American Home Products Corporation | Alcohol-free pneumococcal polysaccharide purification process |
| US6146902A (en) * | 1998-12-29 | 2000-11-14 | Aventis Pasteur, Inc. | Purification of polysaccharide-protein conjugate vaccines by ultrafiltration with ammonium sulfate solutions |
| KR20080081978A (en) * | 2000-03-16 | 2008-09-10 | 더 칠드런스 호스피탈 오브 필라델피아 | Method of regulating capsular polysaccharide production of pneumococcal |
| US6743610B2 (en) * | 2001-03-30 | 2004-06-01 | The University Of Chicago | Method to produce succinic acid from raw hydrolysates |
| ITTS20010013A1 (en) | 2001-06-04 | 2002-12-04 | Ct Ricerche Poly Tec H A R L S | NEW HALURONAN DERIVATIVES. |
| PL1664319T3 (en) * | 2003-09-11 | 2010-07-30 | De Staat Der Nederlanden Vert Door De Mini Van Vws | Process for producing a capsular polysaccharide for use in conjugate vaccines |
| US20070184072A1 (en) * | 2005-04-08 | 2007-08-09 | Wyeth | Multivalent pneumococcal polysaccharide-protein conjugate composition |
| AU2006234984B2 (en) | 2005-04-08 | 2011-10-13 | Wyeth | Separation of contaminants from Streptococcus pneumoniae polysaccharide by pH manipulation |
| US7709001B2 (en) * | 2005-04-08 | 2010-05-04 | Wyeth Llc | Multivalent pneumococcal polysaccharide-protein conjugate composition |
| US7955605B2 (en) | 2005-04-08 | 2011-06-07 | Wyeth Llc | Multivalent pneumococcal polysaccharide-protein conjugate composition |
| CN104815327A (en) * | 2005-04-08 | 2015-08-05 | 惠氏有限责任公司 | Multivalent pneumococcal polysaccharide-protein conjugate composition |
| GB0522303D0 (en) * | 2005-11-01 | 2005-12-07 | Chiron Srl | Culture method |
| PT2074221E (en) * | 2006-10-10 | 2010-09-06 | Wyeth Llc | Improved methods for the separation of streptococcus pneumoniae type 3 polysaccharides |
| US7860500B2 (en) * | 2006-10-27 | 2010-12-28 | Motorola, Inc. | Method and apparatus for determining appropriate channels for communication |
| PT2129693T (en) * | 2007-03-23 | 2017-02-14 | Wyeth Llc | Shortened purification process for the production of capsular streptococcus pneumoniae polysaccharides |
| KR101307703B1 (en) * | 2008-12-18 | 2013-09-11 | 와이어쓰 엘엘씨 | Method for controlling streptococcus pneumoniae polysaccharide molecular weight using carbon |
-
2009
- 2009-12-17 HU HUE09795862A patent/HUE037465T2/en unknown
- 2009-12-17 DK DK09795862.3T patent/DK2379734T3/en active
- 2009-12-17 WO PCT/US2009/068429 patent/WO2010080486A2/en not_active Ceased
- 2009-12-17 PT PT97958623T patent/PT2379734T/en unknown
- 2009-12-17 CA CA2743710A patent/CA2743710C/en active Active
- 2009-12-17 BR BRPI0922981-7 patent/BRPI0922981A8/en not_active Application Discontinuation
- 2009-12-17 RU RU2011128564/10A patent/RU2511404C2/en active
- 2009-12-17 EP EP09795862.3A patent/EP2379734B1/en active Active
- 2009-12-17 SI SI200931830T patent/SI2379734T1/en unknown
- 2009-12-17 CN CN200980150879.5A patent/CN102257155B/en active Active
- 2009-12-17 MX MX2011006432A patent/MX2011006432A/en active IP Right Grant
- 2009-12-17 AU AU2009335826A patent/AU2009335826B2/en active Active
- 2009-12-17 ES ES09795862.3T patent/ES2666698T3/en active Active
- 2009-12-17 KR KR1020137031507A patent/KR101593770B1/en active Active
- 2009-12-17 US US12/640,769 patent/US8795689B2/en active Active
- 2009-12-17 PL PL09795862T patent/PL2379734T3/en unknown
- 2009-12-17 KR KR1020117013836A patent/KR20110091546A/en not_active Ceased
- 2009-12-17 JP JP2011542425A patent/JP5843612B2/en active Active
-
2011
- 2011-05-26 IL IL213169A patent/IL213169A/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| US8795689B2 (en) | 2014-08-05 |
| KR20130136011A (en) | 2013-12-11 |
| WO2010080486A2 (en) | 2010-07-15 |
| JP5843612B2 (en) | 2016-01-13 |
| EP2379734B1 (en) | 2018-03-21 |
| CA2743710C (en) | 2017-01-24 |
| KR20110091546A (en) | 2011-08-11 |
| CA2743710A1 (en) | 2010-07-15 |
| MX2011006432A (en) | 2011-09-29 |
| HUE037465T2 (en) | 2018-09-28 |
| DK2379734T3 (en) | 2018-04-23 |
| RU2011128564A (en) | 2013-01-27 |
| EP2379734A2 (en) | 2011-10-26 |
| ES2666698T3 (en) | 2018-05-07 |
| RU2511404C2 (en) | 2014-04-10 |
| BRPI0922981A2 (en) | 2015-08-25 |
| KR101593770B1 (en) | 2016-02-15 |
| AU2009335826A1 (en) | 2010-07-15 |
| AU2009335826B2 (en) | 2013-10-31 |
| HK1163750A1 (en) | 2012-09-14 |
| CN102257155B (en) | 2016-03-02 |
| IL213169A (en) | 2015-02-26 |
| BRPI0922981A8 (en) | 2019-11-26 |
| WO2010080486A3 (en) | 2010-11-04 |
| JP2012512660A (en) | 2012-06-07 |
| CN102257155A (en) | 2011-11-23 |
| PT2379734T (en) | 2018-04-27 |
| US20100158953A1 (en) | 2010-06-24 |
| PL2379734T3 (en) | 2018-08-31 |
| IL213169A0 (en) | 2011-07-31 |
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