[go: up one dir, main page]

SI1687609T1 - Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem - Google Patents

Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem

Info

Publication number
SI1687609T1
SI1687609T1 SI200432218T SI200432218T SI1687609T1 SI 1687609 T1 SI1687609 T1 SI 1687609T1 SI 200432218 T SI200432218 T SI 200432218T SI 200432218 T SI200432218 T SI 200432218T SI 1687609 T1 SI1687609 T1 SI 1687609T1
Authority
SI
Slovenia
Prior art keywords
hybridization
fluorescent probes
improved sensitivity
low background
dna detection
Prior art date
Application number
SI200432218T
Other languages
English (en)
Inventor
Sergey Lokhov
Eugene Lukhtanov
Original Assignee
Epoch Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epoch Biosciences, Inc. filed Critical Epoch Biosciences, Inc.
Publication of SI1687609T1 publication Critical patent/SI1687609T1/sl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SI200432218T 2003-10-28 2004-10-27 Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem SI1687609T1 (sl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51552303P 2003-10-28 2003-10-28
PCT/US2004/037981 WO2005043127A2 (en) 2003-10-28 2004-10-27 Fluorescent probes for dna detection by hybridization with improved sensitivity and low background
EP04817540.0A EP1687609B1 (en) 2003-10-28 2004-10-27 Fluorescent probes for dna detection by hybridization with improved sensitivity and low background

Publications (1)

Publication Number Publication Date
SI1687609T1 true SI1687609T1 (sl) 2015-03-31

Family

ID=34549422

Family Applications (1)

Application Number Title Priority Date Filing Date
SI200432218T SI1687609T1 (sl) 2003-10-28 2004-10-27 Fluorescenäśne sonde za detekcijo dna s hibridizacijo z izboljĺ ano obäśutljivostjo in nizkim ozadjem

Country Status (11)

Country Link
US (2) US7381818B2 (sl)
EP (1) EP1687609B1 (sl)
JP (1) JP4942484B2 (sl)
AU (1) AU2004286352A1 (sl)
CA (1) CA2542768A1 (sl)
DK (1) DK1687609T3 (sl)
ES (1) ES2529256T3 (sl)
PL (1) PL1687609T3 (sl)
PT (1) PT1687609E (sl)
SI (1) SI1687609T1 (sl)
WO (1) WO2005043127A2 (sl)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE488604T1 (de) 2003-05-20 2010-12-15 Investigen Inc System zum nachweis von polynukleotiden
US7381818B2 (en) * 2003-10-28 2008-06-03 Epoch Biosciences, Inc. Fluorescent probes containing 5′-minor groove binder, fluorophore and quenching moieties and methods of use thereof
EP2010677A4 (en) * 2006-02-24 2010-04-14 Investigen Inc METHODS AND COMPOSITIONS FOR DETECTION OF POLYNUCLEOTIDES
CA2648702A1 (en) * 2006-04-04 2007-10-11 Arcxis Biotechnologies, Inc. Cooperative probes and methods of using them
US8541555B2 (en) * 2006-04-18 2013-09-24 Solulink Biosciences, Inc. Hydrazone-based and oxime-based fluorescent and chromophoric/pro-fluorescent and pro-chromophoric reagents and linkers
WO2008002920A2 (en) * 2006-06-26 2008-01-03 Epoch Biosciences, Inc. Methods for generating target nucleic acid sequences
WO2008103702A2 (en) 2007-02-23 2008-08-28 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
US20090111100A1 (en) * 2007-10-30 2009-04-30 Eugene Lukhtanov Minor groove binder - energy transfer oligonucleotides and methods for their use
WO2010011884A2 (en) * 2008-07-25 2010-01-28 University Of Florida Research Foundation, Inc. Novel nucleic acid-based molecular probes
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
CA2748174A1 (en) 2008-12-30 2010-07-08 Arqule, Inc. Substituted 1h-pyrazolo[3,4-d]pyrimidine-6-amine compounds
US8343961B2 (en) 2009-03-31 2013-01-01 Arqule, Inc. Substituted heterocyclic compounds
CN101659952B (zh) * 2009-07-14 2012-07-18 上海之江生物科技有限公司 锁核酸和小沟结合物共修饰核酸片段
CA2781618A1 (en) * 2009-11-25 2011-06-03 Elitech Holding B.V. Minor groove binder (mgb)-oligonucleotide mirna antagonists
BR112012018394B8 (pt) * 2009-12-21 2021-07-27 Seegene Inc método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo
WO2011087707A1 (en) 2009-12-22 2011-07-21 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
EP3572528A1 (en) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
WO2012119051A2 (en) * 2011-03-02 2012-09-07 Groove Biopharma Corporation Enhanced biodistribution of oligomers
US8969003B2 (en) 2011-03-23 2015-03-03 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
US9085800B2 (en) 2011-03-23 2015-07-21 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
US9677142B2 (en) 2011-05-24 2017-06-13 Elitechgroup B.V. Detection of methicillin-resistant Staphylococcus aureus
JP6182529B2 (ja) 2011-07-26 2017-08-16 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 副溝結合剤ホスホラミダイトおよび使用方法
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
EP2997161B1 (en) 2013-05-13 2017-09-27 Elitechgroup B.V. Droplet digital pcr with short minor groove probes
JP5928906B2 (ja) * 2013-08-27 2016-06-01 横河電機株式会社 核酸配列計測方法、核酸配列計測用デバイス、核酸配列計測用デバイスの製造方法および核酸配列計測装置
JP2017538418A (ja) 2014-12-12 2017-12-28 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 抗生物質耐性細菌を検出する方法および組成物
EP3230467A1 (en) 2014-12-12 2017-10-18 ELITechGroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
EP3208260A1 (en) * 2016-02-17 2017-08-23 Clariant International Ltd Alkoxylated hydroxybenzoic acid esters or amides
US10718017B2 (en) 2016-03-31 2020-07-21 Arizona Board Of Regents On Behalf Of Arizona State University Probes and methods for measuring tandem repeats
IL291323B2 (en) 2016-07-15 2023-09-01 Am Chemicals Llc Solid non-nucleoside supports and phosphoramidite building blocks for oligonucleotide synthesis
US10738346B2 (en) 2017-03-09 2020-08-11 Elitechgroup, Inc. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
EP3668884B1 (en) 2017-08-17 2022-03-09 ELITechGroup Inc. Duplex stabilizing fluorescence quenchers for nucleic acid probes
US20190284609A1 (en) 2018-03-14 2019-09-19 Luminex Corporation Fast, multiplex amplification of nucleic acids
US11155713B2 (en) 2018-05-29 2021-10-26 Elitechgroup, Inc. Carborhodamine compounds and methods of preparation thereof
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2023248185A1 (en) 2022-06-24 2023-12-28 Mobidiag Oy Compact detection system

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
FR2540122B1 (fr) * 1983-01-27 1985-11-29 Centre Nat Rech Scient Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5525464A (en) * 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5585481A (en) * 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1990003370A1 (en) 1988-09-28 1990-04-05 Microprobe Corporation DERIVATIVES OF PYRAZOLO[3,4-d]PYRIMIDINE
US5849482A (en) 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
USRE38416E1 (en) * 1988-09-28 2004-02-03 Epoch Biosciences, Inc. Cross-linking oligonucleotides
JPH04507402A (ja) 1989-05-18 1992-12-24 マイクロプローブ・コーポレーション 架橋オリゴヌクレオチド
US5451463A (en) * 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5237101A (en) * 1990-01-26 1993-08-17 The Trustees Of The Univ. Of Penna. Propargylic and allenic sulfones
WO1992000588A1 (fr) 1990-07-02 1992-01-09 Teijin Limited Disque optique
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
AU2028792A (en) 1991-05-17 1992-12-30 Uab Research Foundation Sequence specific dna binding drugs
US5419966A (en) * 1991-06-10 1995-05-30 Microprobe Corporation Solid support for synthesis of 3'-tailed oligonucleotides
US5512677A (en) * 1991-08-13 1996-04-30 National Science Council 3-substituted methyl-2,3-dihydroimidazo 1,2-c!quinazoline derivatives, the preparation and use thereof
EP0661979A4 (en) 1991-08-21 1995-09-13 Microprobe Corp CROSSLINKED OLIGONUCLEOTIDES FOR ENZYMATIC MEDIATED TRIPLE STRAND FORMATION.
JP2775552B2 (ja) 1991-12-26 1998-07-16 三菱電機株式会社 半導体記憶装置
US5574142A (en) * 1992-12-15 1996-11-12 Microprobe Corporation Peptide linkers for improved oligonucleotide delivery
AU6296294A (en) 1993-01-26 1994-08-15 Microprobe Corporation Bifunctional crosslinking oligonucleotides adapted for linking to a desired gene sequence of invading organism or cell
US5786138A (en) * 1993-01-29 1998-07-28 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Hyperstabilizing antisense nucleic acid binding agents
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5446137B1 (en) * 1993-12-09 1998-10-06 Behringwerke Ag Oligonucleotides containing 4'-substituted nucleotides
US5646126A (en) * 1994-02-28 1997-07-08 Epoch Pharmaceuticals Sterol modified oligonucleotide duplexes having anticancer activity
DE4408528A1 (de) 1994-03-14 1995-09-28 Hoechst Ag Peptid-Oligonucleotid-Derivate, deren Herstellung und Verwendung
FR2719048B1 (fr) 1994-04-25 1996-07-19 Pasteur Strasbourg I Universit Bases nucléiques polycationiques et oligonucléotides les contenant.
US5556752A (en) * 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US6312894B1 (en) * 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5912340A (en) 1995-10-04 1999-06-15 Epoch Pharmaceuticals, Inc. Selective binding complementary oligonucleotides
US5659022A (en) * 1996-01-05 1997-08-19 Epoch Pharmaceuticals, Inc. Oligonucleotide-cyclopropapyrroloindole conjugates as sequence specific hybridization and crosslinking agents for nucleic acids
US5736626A (en) * 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
US5776907A (en) * 1996-05-20 1998-07-07 Texas Biotechnology Corporation Mitomycin oligonucleotide conjugates
US5955590A (en) 1996-07-15 1999-09-21 Worcester Foundation For Biomedical Research Conjugates of minor groove DNA binders with antisense oligonucleotides
US6248518B1 (en) * 1996-10-29 2001-06-19 Board Of Regents Of University Of Nebraska Method for detecting point mutations in DNA utilizing fluorescence energy transfer
US7045610B2 (en) * 1998-04-03 2006-05-16 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US6127121A (en) * 1998-04-03 2000-10-03 Epoch Pharmaceuticals, Inc. Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
US6683173B2 (en) * 1998-04-03 2004-01-27 Epoch Biosciences, Inc. Tm leveling methods
US6486303B1 (en) * 1998-04-14 2002-11-26 University Of Medicine & Dentistry Of New Jersey Method for making hormone heterodimers
US6339147B1 (en) * 1999-07-29 2002-01-15 Epoch Biosciences, Inc. Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture and detection of nucleic acids
US6472153B1 (en) * 1999-10-26 2002-10-29 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
US6660845B1 (en) * 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US7205105B2 (en) * 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US6506568B2 (en) * 2000-02-10 2003-01-14 The Penn State Research Foundation Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
ATE478085T1 (de) * 2000-03-01 2010-09-15 Epoch Biosciences Inc Modifizierte oligonukleotide zur unterscheidung von fehlpaarungen
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US6962991B2 (en) 2001-09-12 2005-11-08 Epoch Biosciences, Inc. Process for the synthesis of pyrazolopyrimidines
WO2003026657A1 (en) 2001-09-24 2003-04-03 Synaptic Pharmaceutical Corporation Compounds for the treatment of pain
WO2003078450A2 (en) * 2002-03-11 2003-09-25 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7348146B2 (en) * 2003-10-02 2008-03-25 Epoch Biosciences, Inc. Single nucleotide polymorphism analysis of highly polymorphic target sequences
US7381818B2 (en) * 2003-10-28 2008-06-03 Epoch Biosciences, Inc. Fluorescent probes containing 5′-minor groove binder, fluorophore and quenching moieties and methods of use thereof

Also Published As

Publication number Publication date
CA2542768A1 (en) 2005-05-12
US20080293586A1 (en) 2008-11-27
EP1687609A2 (en) 2006-08-09
WO2005043127A3 (en) 2006-11-09
US20050214797A1 (en) 2005-09-29
DK1687609T3 (en) 2015-01-12
US7381818B2 (en) 2008-06-03
PL1687609T3 (pl) 2015-05-29
AU2004286352A1 (en) 2005-05-12
JP2007509634A (ja) 2007-04-19
EP1687609A4 (en) 2012-06-20
US7759126B2 (en) 2010-07-20
WO2005043127A2 (en) 2005-05-12
PT1687609E (pt) 2015-03-02
ES2529256T3 (es) 2015-02-18
EP1687609B1 (en) 2014-12-10
JP4942484B2 (ja) 2012-05-30

Similar Documents

Publication Publication Date Title
PT1687609E (pt) Sondas fluorescentes para a detecção de adn por hibridação com uma maior sensibilidade e menor ruído de fundo
EP2484781A3 (en) Detection of analytes and nucleic acids
EP1855102A4 (en) FLUORESCENCE DETECTION DEVICE AND FLUORESCENCE DETECTION METHOD
EP1817573A4 (en) FLUORESCENCE DETECTION
EP2266996A3 (en) New labelling strategies for the sensitive detection of analytes
AU2002222614A1 (en) Method of detecting polymorphism in dna by using mass spectroscopy
EP2037272A4 (en) KIT AND METHOD FOR DETECTING OR BZW. FOR QUANTIFYING AN ANALYTE
EP1745120A4 (en) SYSTEM FOR RAPID AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS
IL175628A0 (en) Sample collection cup with integrated sample analysis
AP2006003813A0 (en) Nano-pcr: methods and devices for nucleic acid amplification and detection
DE602005009702D1 (de) Esr1 und gebärmutterhalskrebs
EP1613959A4 (en) PROCESS FOR DETECTING CANCER CELLS IN BIOLOGICAL SAMPLES
EP1994170A4 (en) KITS AND METHOD FOR DETECTION OF PAPILLOMA HUMAN VIRUSES WITH NUCLEOTIDE CORD ANALYSIS
EP1673476A4 (en) DEVICE AND METHOD FOR DETECTING NUCLEIC ACID IN BIOLOGICAL SAMPLES
EP1692266A4 (en) IMPROVED METHODS FOR DETECTING AND MEASURING SPECIFIC NUCLEIC ACID SEQUENCES
PL1895015T3 (pl) Sposób i zestaw do wykrywania gatunków bakteryjnych za pomocą analizy DNA
EP1620556A4 (en) NUCLEIC PROOF
GB0505777D0 (en) Enzyme detection method and reagent
EP1804788A4 (en) DETECTION AND ANALYSIS OF FLUORESCENT MOLECULES RELATING TO OPHTHALMOLOGY
ZA200708154B (en) Nucleic acid detection
WO2004000995A3 (en) Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms
EP1546392A4 (en) DETECTION OF SPECIFIC DNA FROM SPECIES
AU2003290433A8 (en) Nucleic acid methylation detection process using an internal reference sample
ZA200903128B (en) A high sensitivity assay for molecular typing of biological sample, probes and a kit thereof
ZA200601731B (en) Methods for detecting and quantifying specific microorganisms