[go: up one dir, main page]

SG11202112703QA - Array and method for detecting spatial information of nucleic acids - Google Patents

Array and method for detecting spatial information of nucleic acids

Info

Publication number
SG11202112703QA
SG11202112703QA SG11202112703QA SG11202112703QA SG11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA
Authority
SG
Singapore
Prior art keywords
array
nucleic acids
spatial information
detecting spatial
detecting
Prior art date
Application number
SG11202112703QA
Inventor
Ao Chen
Xun Xu
Jin Yang
Longqi Liu
Ou Wang
Yuxiang Li
Sha Liao
Guoxin Tang
Yuan Jiang
Chongjun Xu
Ming Ni
Wenwei Zhang
Radoje Drmanac
Snezana Drmanac
Original Assignee
Bgi Shenzhen
Mgi Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bgi Shenzhen, Mgi Tech Co Ltd filed Critical Bgi Shenzhen
Publication of SG11202112703QA publication Critical patent/SG11202112703QA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00Methods specially adapted for identifying library members
    • C40B20/02Identifying library members by their fixed physical location on a support or substrate
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101Primer extension

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202112703QA 2019-05-15 2020-05-14 Array and method for detecting spatial information of nucleic acids SG11202112703QA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201910403775 2019-05-15
CN201911240733 2019-12-06
PCT/CN2020/090340 WO2020228788A1 (en) 2019-05-15 2020-05-14 Array and method for detecting spatial information of nucleic acids

Publications (1)

Publication Number Publication Date
SG11202112703QA true SG11202112703QA (en) 2021-12-30

Family

ID=73289590

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202112703QA SG11202112703QA (en) 2019-05-15 2020-05-14 Array and method for detecting spatial information of nucleic acids

Country Status (11)

Country Link
US (1) US20230175047A1 (en)
EP (1) EP3971282A4 (en)
JP (2) JP2022540744A (en)
KR (1) KR20220009445A (en)
CN (9) CN114207105B (en)
AU (1) AU2020274774C1 (en)
BR (1) BR112021022865A2 (en)
CA (1) CA3140512A1 (en)
MX (1) MX2021013907A (en)
SG (1) SG11202112703QA (en)
WO (1) WO2020228788A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118434882A (en) * 2021-12-24 2024-08-02 深圳华大生命科学研究院 A method for generating a labeled nucleic acid molecule population and a kit thereof
US20250129407A1 (en) * 2021-12-24 2025-04-24 Bgi Shenzhen Method for generating labeled nucleic acid molecular population and kit thereof
JP2024546177A (en) * 2021-12-24 2024-12-17 ビージーアイ シェンチェン Methods for labeling and analyzing single-cell nucleic acids
EP4455259A4 (en) * 2021-12-24 2025-10-22 Bgi Shenzhen DNA library sequence for increasing the number of chip probes and its application
CN114836415B (en) * 2022-05-06 2023-04-18 南京诺唯赞生物科技股份有限公司 Preparation of MGI platform transposase double-end tag library
WO2024065585A1 (en) * 2022-09-30 2024-04-04 深圳华大生命科学研究院 Biochip and use and preparation method therefor
CN117210943B (en) * 2023-10-11 2025-09-05 中国科学院广州生物医药与健康研究院 Methods for constructing spatial transcriptome chips and cDNA libraries and methods for transcriptome sequencing analysis
WO2025138284A1 (en) * 2023-12-29 2025-07-03 深圳华大生命科学研究院 Array and method for detecting spatial information of nucleic acids
WO2025162175A1 (en) * 2024-02-01 2025-08-07 深圳赛陆医疗科技有限公司 Ultrahigh-density probe capture-based spatial transcriptomics chip, preparation method therefor, and use thereof

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITBO20070627A1 (en) * 2007-09-14 2009-03-15 Twof Inc METHOD FOR THE PREPARATION OF MICROARRAY DNA WITH HIGH LINEAR DENSITY PROBES
AU2008335362B2 (en) * 2007-12-05 2013-07-25 Complete Genomics, Inc. Efficient base determination in sequencing reactions
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
SI2556171T1 (en) * 2010-04-05 2016-03-31 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9671344B2 (en) 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9834814B2 (en) * 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
CN105986324B (en) * 2015-02-11 2018-08-14 深圳华大智造科技有限公司 Cyclic annular tiny RNA library constructing method and its application
DK3901281T4 (en) * 2015-04-10 2025-10-20 Illumina Inc SPATIALLY SEPARATED, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SAMPLES
WO2016168825A1 (en) * 2015-04-17 2016-10-20 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological molecules
CN108138225B (en) * 2015-07-27 2022-10-14 亿明达股份有限公司 Spatial localization of nucleic acid sequence information
US11339390B2 (en) * 2015-09-11 2022-05-24 The Broad Institute, Inc. DNA microscopy methods
CN105505755A (en) * 2015-12-23 2016-04-20 杭州谷禾信息技术有限公司 Space transcriptome database building and sequencing method and device adopted for same
WO2018075436A1 (en) * 2016-10-17 2018-04-26 West Jason A A High resolution spatial genomic analysis of tissues and cell aggregates

Also Published As

Publication number Publication date
CN119753087A (en) 2025-04-04
JP2024156848A (en) 2024-11-06
CA3140512A1 (en) 2020-11-19
AU2020274774B2 (en) 2024-06-20
BR112021022865A2 (en) 2022-01-25
CN119530345A (en) 2025-02-28
AU2020274774C1 (en) 2024-12-05
JP2022540744A (en) 2022-09-20
MX2021013907A (en) 2022-05-06
CN114207105A (en) 2022-03-18
EP3971282A4 (en) 2023-12-27
CN119530344A (en) 2025-02-28
CN119592665A (en) 2025-03-11
CN114207105B (en) 2024-12-20
CN119592664A (en) 2025-03-11
CN119530346A (en) 2025-02-28
WO2020228788A1 (en) 2020-11-19
CN119685448A (en) 2025-03-25
EP3971282A1 (en) 2022-03-23
CN119530347A (en) 2025-02-28
KR20220009445A (en) 2022-01-24
US20230175047A1 (en) 2023-06-08
AU2020274774A1 (en) 2022-01-20

Similar Documents

Publication Publication Date Title
SG11202112703QA (en) Array and method for detecting spatial information of nucleic acids
SG11202111878RA (en) Method of detecting target nucleic acid molecules
SG11202105109PA (en) Method for detection of rna
CA3288886A1 (en) System and method for protein corona sensor array for early detection of diseases
ZA202104864B (en) Nucleic acid sequence for detecting maize plant dbn9501 and detection method therefor
EP3640340A4 (en) METHOD OF DETECTION OF TARGET NUCLEIC ACID SEQUENCES
ZA201808277B (en) System and method for secondary analysis of nucleotide sequencing data
ZA202108689B (en) Nucleic acid sequence for detecting soybean plant dbn8002 and detection method therefor
GB201909211D0 (en) System and method for automated detection of clinical outcome measures
IL277870A (en) Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
PT4060050T (en) Highly sensitive methods for accurate parallel quantification of nucleic acids
SG10201910254YA (en) Nucleic Acid Detection Method Using Lamp And Probe Detection
GB202112088D0 (en) Nucleic acid detection
GB201804642D0 (en) Methods of labelling nucleic acids
EP3457133A4 (en) Diagnostic marker for dementia and method for identifying contraction of dementia using said marker
EP4061958A4 (en) Methods for sequential detection of nucleic acids
EP3650553C0 (en) METHOD FOR DETECTING SPECIFIC NUCLEIC ACIDS
IL277764B (en) Method for detecting variation of reference sequence in target nucleic acid region
SG11201708920PA (en) Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same
AU2019405977B2 (en) Compositions and methods for detecting Plasmodium species nucleic acid
HK40117729A (en) Array and detection method for detecting nucleic acid spatial information
HK40117219A (en) Array and detection method for detecting nucleic acid spatial information
HK40116511A (en) Array and detection method for detecting nucleic acid spatial information
HK40116450A (en) Array and detection method for detecting nucleic acid spatial information
GB202020118D0 (en) Compositions and methods for tagging and detecting nucleic acids