SG11202112703QA - Array and method for detecting spatial information of nucleic acids - Google Patents
Array and method for detecting spatial information of nucleic acidsInfo
- Publication number
- SG11202112703QA SG11202112703QA SG11202112703QA SG11202112703QA SG11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA SG 11202112703Q A SG11202112703Q A SG 11202112703QA
- Authority
- SG
- Singapore
- Prior art keywords
- array
- nucleic acids
- spatial information
- detecting spatial
- detecting
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/02—Identifying library members by their fixed physical location on a support or substrate
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/101—Primer extension
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910403775 | 2019-05-15 | ||
| CN201911240733 | 2019-12-06 | ||
| PCT/CN2020/090340 WO2020228788A1 (en) | 2019-05-15 | 2020-05-14 | Array and method for detecting spatial information of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202112703QA true SG11202112703QA (en) | 2021-12-30 |
Family
ID=73289590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202112703QA SG11202112703QA (en) | 2019-05-15 | 2020-05-14 | Array and method for detecting spatial information of nucleic acids |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20230175047A1 (en) |
| EP (1) | EP3971282A4 (en) |
| JP (2) | JP2022540744A (en) |
| KR (1) | KR20220009445A (en) |
| CN (9) | CN114207105B (en) |
| AU (1) | AU2020274774C1 (en) |
| BR (1) | BR112021022865A2 (en) |
| CA (1) | CA3140512A1 (en) |
| MX (1) | MX2021013907A (en) |
| SG (1) | SG11202112703QA (en) |
| WO (1) | WO2020228788A1 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118434882A (en) * | 2021-12-24 | 2024-08-02 | 深圳华大生命科学研究院 | A method for generating a labeled nucleic acid molecule population and a kit thereof |
| US20250129407A1 (en) * | 2021-12-24 | 2025-04-24 | Bgi Shenzhen | Method for generating labeled nucleic acid molecular population and kit thereof |
| JP2024546177A (en) * | 2021-12-24 | 2024-12-17 | ビージーアイ シェンチェン | Methods for labeling and analyzing single-cell nucleic acids |
| EP4455259A4 (en) * | 2021-12-24 | 2025-10-22 | Bgi Shenzhen | DNA library sequence for increasing the number of chip probes and its application |
| CN114836415B (en) * | 2022-05-06 | 2023-04-18 | 南京诺唯赞生物科技股份有限公司 | Preparation of MGI platform transposase double-end tag library |
| WO2024065585A1 (en) * | 2022-09-30 | 2024-04-04 | 深圳华大生命科学研究院 | Biochip and use and preparation method therefor |
| CN117210943B (en) * | 2023-10-11 | 2025-09-05 | 中国科学院广州生物医药与健康研究院 | Methods for constructing spatial transcriptome chips and cDNA libraries and methods for transcriptome sequencing analysis |
| WO2025138284A1 (en) * | 2023-12-29 | 2025-07-03 | 深圳华大生命科学研究院 | Array and method for detecting spatial information of nucleic acids |
| WO2025162175A1 (en) * | 2024-02-01 | 2025-08-07 | 深圳赛陆医疗科技有限公司 | Ultrahigh-density probe capture-based spatial transcriptomics chip, preparation method therefor, and use thereof |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITBO20070627A1 (en) * | 2007-09-14 | 2009-03-15 | Twof Inc | METHOD FOR THE PREPARATION OF MICROARRAY DNA WITH HIGH LINEAR DENSITY PROBES |
| AU2008335362B2 (en) * | 2007-12-05 | 2013-07-25 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| WO2009132028A1 (en) * | 2008-04-21 | 2009-10-29 | Complete Genomics, Inc. | Array structures for nucleic acid detection |
| SI2556171T1 (en) * | 2010-04-05 | 2016-03-31 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US9671344B2 (en) | 2010-08-31 | 2017-06-06 | Complete Genomics, Inc. | High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging |
| GB201106254D0 (en) * | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US9834814B2 (en) * | 2013-11-22 | 2017-12-05 | Agilent Technologies, Inc. | Spatial molecular barcoding of in situ nucleic acids |
| CN105986324B (en) * | 2015-02-11 | 2018-08-14 | 深圳华大智造科技有限公司 | Cyclic annular tiny RNA library constructing method and its application |
| DK3901281T4 (en) * | 2015-04-10 | 2025-10-20 | Illumina Inc | SPATIALLY SEPARATED, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SAMPLES |
| WO2016168825A1 (en) * | 2015-04-17 | 2016-10-20 | Centrillion Technology Holdings Corporation | Methods for performing spatial profiling of biological molecules |
| CN108138225B (en) * | 2015-07-27 | 2022-10-14 | 亿明达股份有限公司 | Spatial localization of nucleic acid sequence information |
| US11339390B2 (en) * | 2015-09-11 | 2022-05-24 | The Broad Institute, Inc. | DNA microscopy methods |
| CN105505755A (en) * | 2015-12-23 | 2016-04-20 | 杭州谷禾信息技术有限公司 | Space transcriptome database building and sequencing method and device adopted for same |
| WO2018075436A1 (en) * | 2016-10-17 | 2018-04-26 | West Jason A A | High resolution spatial genomic analysis of tissues and cell aggregates |
-
2020
- 2020-05-14 MX MX2021013907A patent/MX2021013907A/en unknown
- 2020-05-14 CA CA3140512A patent/CA3140512A1/en active Pending
- 2020-05-14 CN CN202080036185.5A patent/CN114207105B/en active Active
- 2020-05-14 CN CN202411732967.9A patent/CN119592664A/en active Pending
- 2020-05-14 CN CN202411741948.2A patent/CN119753087A/en active Pending
- 2020-05-14 CN CN202411740879.3A patent/CN119530346A/en active Pending
- 2020-05-14 CN CN202411741950.XA patent/CN119685448A/en active Pending
- 2020-05-14 US US17/611,099 patent/US20230175047A1/en active Pending
- 2020-05-14 CN CN202411741603.7A patent/CN119592665A/en active Pending
- 2020-05-14 EP EP20806227.3A patent/EP3971282A4/en active Pending
- 2020-05-14 CN CN202411741947.8A patent/CN119530347A/en active Pending
- 2020-05-14 KR KR1020217041221A patent/KR20220009445A/en active Pending
- 2020-05-14 BR BR112021022865A patent/BR112021022865A2/en unknown
- 2020-05-14 CN CN202411737361.4A patent/CN119530345A/en active Pending
- 2020-05-14 WO PCT/CN2020/090340 patent/WO2020228788A1/en not_active Ceased
- 2020-05-14 SG SG11202112703QA patent/SG11202112703QA/en unknown
- 2020-05-14 CN CN202411735916.1A patent/CN119530344A/en active Pending
- 2020-05-14 JP JP2021568140A patent/JP2022540744A/en active Pending
- 2020-05-14 AU AU2020274774A patent/AU2020274774C1/en active Active
-
2024
- 2024-08-01 JP JP2024126026A patent/JP2024156848A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN119753087A (en) | 2025-04-04 |
| JP2024156848A (en) | 2024-11-06 |
| CA3140512A1 (en) | 2020-11-19 |
| AU2020274774B2 (en) | 2024-06-20 |
| BR112021022865A2 (en) | 2022-01-25 |
| CN119530345A (en) | 2025-02-28 |
| AU2020274774C1 (en) | 2024-12-05 |
| JP2022540744A (en) | 2022-09-20 |
| MX2021013907A (en) | 2022-05-06 |
| CN114207105A (en) | 2022-03-18 |
| EP3971282A4 (en) | 2023-12-27 |
| CN119530344A (en) | 2025-02-28 |
| CN119592665A (en) | 2025-03-11 |
| CN114207105B (en) | 2024-12-20 |
| CN119592664A (en) | 2025-03-11 |
| CN119530346A (en) | 2025-02-28 |
| WO2020228788A1 (en) | 2020-11-19 |
| CN119685448A (en) | 2025-03-25 |
| EP3971282A1 (en) | 2022-03-23 |
| CN119530347A (en) | 2025-02-28 |
| KR20220009445A (en) | 2022-01-24 |
| US20230175047A1 (en) | 2023-06-08 |
| AU2020274774A1 (en) | 2022-01-20 |
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