SG11202106900TA - Transposase with enhanced insertion site selection properties - Google Patents
Transposase with enhanced insertion site selection propertiesInfo
- Publication number
- SG11202106900TA SG11202106900TA SG11202106900TA SG11202106900TA SG11202106900TA SG 11202106900T A SG11202106900T A SG 11202106900TA SG 11202106900T A SG11202106900T A SG 11202106900TA SG 11202106900T A SG11202106900T A SG 11202106900TA SG 11202106900T A SG11202106900T A SG 11202106900TA
- Authority
- SG
- Singapore
- Prior art keywords
- transposase
- insertion site
- site selection
- selection properties
- enhanced insertion
- Prior art date
Links
- 102000008579 Transposases Human genes 0.000 title 1
- 108010020764 Transposases Proteins 0.000 title 1
- 230000037431 insertion Effects 0.000 title 1
- 238000003780 insertion Methods 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01048—Histone acetyltransferase (2.3.1.48)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Insects & Arthropods (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2019/053571 WO2020164702A1 (en) | 2019-02-13 | 2019-02-13 | Transposase with enhanced insertion site selection properties |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202106900TA true SG11202106900TA (en) | 2021-07-29 |
Family
ID=65443839
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202106900TA SG11202106900TA (en) | 2019-02-13 | 2019-02-13 | Transposase with enhanced insertion site selection properties |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20220090142A1 (en) |
| EP (1) | EP3924474A1 (en) |
| JP (2) | JP7390385B2 (en) |
| KR (1) | KR102816410B1 (en) |
| CN (1) | CN113439121B (en) |
| AU (1) | AU2019429609B2 (en) |
| CA (1) | CA3126773A1 (en) |
| SG (1) | SG11202106900TA (en) |
| WO (1) | WO2020164702A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728477B (en) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | An efficient transposition mutation system and construction method |
| SG11202106900TA (en) * | 2019-02-13 | 2021-07-29 | Probiogen Ag | Transposase with enhanced insertion site selection properties |
| WO2024124197A2 (en) * | 2022-12-09 | 2024-06-13 | Metagenomi, Inc. | Retrotransposon compositions and methods of use |
| CN118185901B (en) * | 2022-12-12 | 2025-03-21 | 北京精缮生物科技有限责任公司 | A PBase protein, fusion protein, nucleic acid and gene integration system and application |
| GB202305637D0 (en) | 2023-04-17 | 2023-05-31 | Macomics Ltd | Transposons, vectors and genetically engineered cells |
| WO2024229342A1 (en) * | 2023-05-04 | 2024-11-07 | Saliogen Therapeutics, Inc. | Piggybac transposase engineering |
| CN118186001A (en) * | 2024-03-12 | 2024-06-14 | 上海市东方医院（同济大学附属东方医院） | Improved piggyBac transposon mediated gene integration method and application thereof |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6218185B1 (en) | 1996-04-19 | 2001-04-17 | The United States Of America As Represented By The Secretary Of Agriculture | Piggybac transposon-based genetic transformation system for insects |
| JP2004519211A (en) * | 2000-07-21 | 2004-07-02 | シンジェンタ パーティシペーションズ アーゲー | Zinc finger domain recognition code and uses thereof |
| KR100985678B1 (en) * | 2002-01-09 | 2010-10-05 | 에라스무스 유니버시티 메디컬 센터 로테르담 | How to induce translocation by translocation vector in transformed organism |
| ES2358994T3 (en) * | 2002-07-24 | 2011-05-17 | Manoa Biosciences Inc. | VECTORS BASED ON TRANSPOSONS AND METHODS FOR THE INTEGRATION OF NUCLEIC ACIDS. |
| CN1215178C (en) * | 2003-01-08 | 2005-08-17 | 中国人民解放军军事医学科学院生物工程研究所 | Genetic-sorting model of zinc finger-protein-DNA interaction and use thereof |
| DE602004031325D1 (en) * | 2003-02-10 | 2011-03-24 | Max Delbrueck Centrum | TRANSPOSON SYSTEM FOR TARGETED INTEGRATION |
| US20060252140A1 (en) * | 2005-04-29 | 2006-11-09 | Yant Stephen R | Development of a transposon system for site-specific DNA integration in mammalian cells |
| WO2008027384A1 (en) * | 2006-08-28 | 2008-03-06 | University Of Hawaii | Methods and compositions for transposon-mediated transgenesis |
| WO2009003671A2 (en) | 2007-07-04 | 2009-01-08 | Max-Delbrück-Centrum für Molekulare Medizin | Hyperactive variants of the transposase protein of the transposon system sleeping beauty |
| MX2010005693A (en) * | 2007-12-03 | 2010-06-01 | Syngenta Participations Ag | Engineering enzymatically susceptible proteins. |
| SG174155A1 (en) | 2009-02-25 | 2011-10-28 | Univ Johns Hopkins | Piggybac transposon variants and methods of use |
| US8399643B2 (en) | 2009-02-26 | 2013-03-19 | Transposagen Biopharmaceuticals, Inc. | Nucleic acids encoding hyperactive PiggyBac transposases |
| CA2838844A1 (en) * | 2011-06-10 | 2012-12-13 | The Trustees Of Columbia University In The City Of New York | Uses of histone acetyltransferase activators |
| GB2493982A (en) * | 2011-08-26 | 2013-02-27 | Univ Leicester | Disease treatments involving histone deacetylases |
| US20130196867A1 (en) * | 2011-12-16 | 2013-08-01 | The University Of North Carolina At Chapel Hill | Combinatorial post-translationally-modified histone peptides, arrays thereof, and methods of using the same |
| GB201212816D0 (en) * | 2012-07-19 | 2012-09-05 | Eisai Ltd | Novel compounds |
| EP3712267A1 (en) * | 2013-05-22 | 2020-09-23 | Active Motif, Inc. | Targeted transposition for use in epigenetic studies |
| CN103981204B (en) * | 2014-03-24 | 2016-05-04 | 上海海洋大学 | A kind of expression of activated goldfish Tgf2 transposons restructuring transposase albumen |
| LT3129487T (en) | 2014-04-09 | 2020-12-28 | Dna Twopointo Inc. | Enhanced nucleic acid constructs for eukaryotic gene expression |
| CN104402982B (en) * | 2014-12-03 | 2018-06-08 | 复旦大学 | Rice istone lysine modification identification albumen and its encoding gene and application |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| CN105886530A (en) * | 2016-04-06 | 2016-08-24 | 中国农业大学 | Construction method of BHK-21 cell line with efficient PRRSV susceptibility |
| WO2018014038A1 (en) * | 2016-07-15 | 2018-01-18 | Poseida Therapeutics, Inc. | Chimeric antigen receptors and methods for use |
| EP3500675A4 (en) * | 2016-08-19 | 2020-01-29 | Whitehead Institute for Biomedical Research | METHODS FOR EDITING DNA METHYLATION |
| WO2018110471A1 (en) * | 2016-12-12 | 2018-06-21 | アステラス製薬株式会社 | Transcription-regulating fusion polypeptide |
| CA3124482A1 (en) * | 2018-12-21 | 2020-06-25 | Epicypher, Inc. | Dna-barcoded nucleosomes for chromatin mapping assays |
| SG11202106900TA (en) * | 2019-02-13 | 2021-07-29 | Probiogen Ag | Transposase with enhanced insertion site selection properties |
| JP2023525007A (en) * | 2020-05-04 | 2023-06-14 | サリオジェン セラピューティクス インコーポレイテッド | Dislocation-based therapy |
| AU2020458769B2 (en) * | 2020-07-17 | 2025-08-21 | Probiogen Ag | Hyperactive transposons and transposases |
| EP4479747A1 (en) * | 2022-02-15 | 2024-12-25 | Epicypher, Inc. | Engineered recombinant protein-binding domains as detection reagents |
-
2019
- 2019-02-13 SG SG11202106900TA patent/SG11202106900TA/en unknown
- 2019-02-13 JP JP2021547089A patent/JP7390385B2/en active Active
- 2019-02-13 CA CA3126773A patent/CA3126773A1/en active Pending
- 2019-02-13 KR KR1020217023370A patent/KR102816410B1/en active Active
- 2019-02-13 AU AU2019429609A patent/AU2019429609B2/en active Active
- 2019-02-13 EP EP19705736.7A patent/EP3924474A1/en active Pending
- 2019-02-13 US US17/420,076 patent/US20220090142A1/en active Pending
- 2019-02-13 WO PCT/EP2019/053571 patent/WO2020164702A1/en not_active Ceased
- 2019-02-13 CN CN201980091933.7A patent/CN113439121B/en active Active
-
2023
- 2023-08-04 JP JP2023127564A patent/JP2023145729A/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| CN113439121B (en) | 2024-12-06 |
| JP2022523166A (en) | 2022-04-21 |
| AU2019429609A1 (en) | 2021-07-22 |
| WO2020164702A1 (en) | 2020-08-20 |
| KR102816410B1 (en) | 2025-06-04 |
| US20220090142A1 (en) | 2022-03-24 |
| AU2019429609B2 (en) | 2025-09-25 |
| JP2023145729A (en) | 2023-10-11 |
| CA3126773A1 (en) | 2020-08-20 |
| KR20210126564A (en) | 2021-10-20 |
| JP7390385B2 (en) | 2023-12-01 |
| CN113439121A (en) | 2021-09-24 |
| EP3924474A1 (en) | 2021-12-22 |
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