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SG11202003885UA - Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters - Google Patents

Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters

Info

Publication number
SG11202003885UA
SG11202003885UA SG11202003885UA SG11202003885UA SG11202003885UA SG 11202003885U A SG11202003885U A SG 11202003885UA SG 11202003885U A SG11202003885U A SG 11202003885UA SG 11202003885U A SG11202003885U A SG 11202003885UA SG 11202003885U A SG11202003885U A SG 11202003885UA
Authority
SG
Singapore
Prior art keywords
adapters
reagents
making
methods
nucleic acid
Prior art date
Application number
SG11202003885UA
Inventor
Jesse J Salk
Scott R Kennedy
Original Assignee
Twinstrand Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Twinstrand Biosciences Inc filed Critical Twinstrand Biosciences Inc
Publication of SG11202003885UA publication Critical patent/SG11202003885UA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11202003885UA 2017-11-08 2018-11-08 Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters SG11202003885UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762583462P 2017-11-08 2017-11-08
PCT/US2018/059908 WO2019094651A1 (en) 2017-11-08 2018-11-08 Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters

Publications (1)

Publication Number Publication Date
SG11202003885UA true SG11202003885UA (en) 2020-05-28

Family

ID=66438656

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202003885UA SG11202003885UA (en) 2017-11-08 2018-11-08 Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters

Country Status (7)

Country Link
US (2) US11739367B2 (en)
EP (2) EP3707273B1 (en)
AU (1) AU2018366213B2 (en)
CA (1) CA3081027A1 (en)
ES (1) ES2977793T3 (en)
SG (1) SG11202003885UA (en)
WO (1) WO2019094651A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
JP6975507B2 (en) 2015-12-08 2021-12-01 ツインストランド・バイオサイエンシズ・インコーポレイテッドTwinstrand Biosciences, Inc. Improved adapters, methods, and compositions for double-stranded sequencing
EP3571616B1 (en) 2017-01-18 2021-05-19 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
EP3619326A1 (en) 2017-05-01 2020-03-11 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
AU2018266377B2 (en) 2017-05-08 2024-06-20 Illumina, Inc. Universal short adapters for indexing of polynucleotide samples
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
BR112021000409A2 (en) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. METHODS AND REAGENTS TO CHARACTERIZE GENOMIC EDITION, CLONAL EXPANSION AND ASSOCIATED APPLICATIONS
CN112930405B (en) 2019-01-11 2025-01-28 Illumina剑桥有限公司 Composite surface-bound transposome complex
US20220372472A1 (en) * 2019-11-05 2022-11-24 Ultima Genomics, Inc. Methods for preparing a library of polynucleotide molecules
CN115348896A (en) * 2020-02-02 2022-11-15 阿尔缇玛基因组学公司 Nucleic acid molecules comprising cleavable or excisable moieties
WO2022109389A1 (en) * 2020-11-20 2022-05-27 Camena Bioscience Limited Geometric synthesis methods and compositions for double-stranded nucleic acid sequencing

Family Cites Families (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5308751A (en) 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
DK0651825T3 (en) 1992-07-06 1998-09-14 Harvard College Methods and diagnostic sets for determining the toxicity of a compound using a bacterial stress
JP2002503954A (en) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド Nucleic acid amplification method
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
DE69929542T2 (en) 1998-10-27 2006-09-14 Affymetrix, Inc., Santa Clara COMPLEXITY MANAGEMENT AND ANALYSIS OF GENOMIC DNA
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
WO2001040516A2 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
ES2661167T3 (en) 2001-10-15 2018-03-27 Bioarray Solutions Ltd. Multiplexed analysis of polymorphic loci by simultaneous consultation and enzyme-mediated detection
US7406385B2 (en) 2001-10-25 2008-07-29 Applera Corporation System and method for consensus-calling with per-base quality values for sample assemblies
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
WO2004065582A2 (en) 2003-01-15 2004-08-05 Dana-Farber Cancer Institute, Inc. Amplification of dna in a hairpin structure, and applications
WO2004081183A2 (en) 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
JP2007524410A (en) 2004-01-23 2007-08-30 リングヴィテ エーエス Improved polynucleotide ligation reaction
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US8796441B2 (en) 2005-04-13 2014-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Human sweet and umami taste receptor variants
CN101641449B (en) 2005-06-23 2014-01-29 科因股份有限公司 Strategies for high-throughput identification and detection of polymorphisms
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
AU2006295556B2 (en) 2005-09-29 2012-07-05 Keygene N.V. High throughput screening of mutagenized populations
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070172839A1 (en) 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
WO2007114693A2 (en) 2006-04-04 2007-10-11 Keygene N.V. High throughput detection of molecular markers based on aflp and high throughput sequencing
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
CA2697640C (en) 2007-09-21 2016-06-21 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
EP4230747A3 (en) 2008-03-28 2023-11-15 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110301042A1 (en) 2008-11-11 2011-12-08 Helicos Biosciences Corporation Methods of sample encoding for multiplex analysis of samples by single molecule sequencing
KR101797773B1 (en) 2009-01-30 2017-11-15 옥스포드 나노포어 테크놀로지즈 리미티드 Adaptors for nucleic acid constructs in transmembrane sequencing
US20100331204A1 (en) 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
GB0905410D0 (en) 2009-03-28 2009-05-13 Gentronix Ltd Genotoxicity testing
US20120165202A1 (en) 2009-04-30 2012-06-28 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
WO2010148115A1 (en) 2009-06-18 2010-12-23 The Penn State Research Foundation Methods, systems and kits for detecting protein-nucleic acid interactions
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CN102933721B (en) 2010-06-09 2015-12-02 凯津公司 Combinatorial sequence barcoding for high-throughput screening
WO2012012037A1 (en) * 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use
US20120244525A1 (en) 2010-07-19 2012-09-27 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
CN103154273A (en) 2010-09-21 2013-06-12 群体遗传学科技有限公司 Improving Confidence in Allele Calls with Molecular Counts
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
DK2652155T3 (en) 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012129242A2 (en) 2011-03-23 2012-09-27 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
CN103748236B (en) 2011-04-15 2018-12-25 约翰·霍普金斯大学 Safe sequencing system
ES2556580T3 (en) 2011-07-08 2016-01-19 Keygene N.V. Genotyping based on sequences based on oligonucleotide ligation assays
US10767208B2 (en) 2011-09-06 2020-09-08 Gen-Probe Incorporated Closed nucleic acid structures
US9249460B2 (en) 2011-09-09 2016-02-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US20160153039A1 (en) 2012-01-26 2016-06-02 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
SG10201504490QA (en) 2012-01-26 2015-07-30 Nugen Technologies Inc Compositions And Methods For Targeted Nucleic Acid Sequence Enrichment And High Efficiency Library Generation
US9528107B2 (en) 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
PT2814959T (en) 2012-02-17 2018-04-12 Hutchinson Fred Cancer Res Compositions and methods for accurately identifying mutations
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
EP3287531B1 (en) 2012-02-28 2019-06-19 Agilent Technologies, Inc. Method for attaching a counter sequence to a nucleic acid sample
CA2867293C (en) 2012-03-13 2020-09-01 Abhijit Ajit PATEL Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
ES2828661T3 (en) 2012-03-20 2021-05-27 Univ Washington Through Its Center For Commercialization Methods to Reduce the Error Rate of Parallel Massive DNA Sequencing Using Double-stranded Consensus Sequence Sequencing
US9487828B2 (en) 2012-05-10 2016-11-08 The General Hospital Corporation Methods for determining a nucleotide sequence contiguous to a known target nucleotide sequence
WO2013181170A1 (en) 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
DE202013012824U1 (en) 2012-09-04 2020-03-10 Guardant Health, Inc. Systems for the detection of rare mutations and a copy number variation
CN102877136B (en) 2012-09-24 2014-03-12 上海交通大学 Genome simplification and next-generation sequencing-based deoxyribose nucleic acid (DNA) library preparation method and kit
AU2013337616B2 (en) 2012-11-05 2019-03-21 Gmdx Co Pty Ltd Methods for determining the cause of somatic mutagenesis
DK3553175T3 (en) 2013-03-13 2021-08-23 Illumina Inc PROCEDURE FOR MAKING A NUCLEIC ACID SEQUENCE LIBRARY
US10087481B2 (en) 2013-03-19 2018-10-02 New England Biolabs, Inc. Enrichment of target sequences
US11414695B2 (en) 2013-05-29 2022-08-16 Agilent Technologies, Inc. Nucleic acid enrichment using Cas9
WO2015075056A1 (en) 2013-11-19 2015-05-28 Thermo Fisher Scientific Baltics Uab Programmable enzymes for isolation of specific dna fragments
EP3771745A1 (en) 2013-12-28 2021-02-03 Guardant Health, Inc. Methods and systems for detecting genetic variants
KR102321956B1 (en) 2014-01-31 2021-11-08 스위프트 바이오사이언시스 인코포레이티드 Improved methods for processing dna substrates
US11085084B2 (en) 2014-09-12 2021-08-10 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acids
US10844428B2 (en) * 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US10465241B2 (en) 2015-06-15 2019-11-05 The Board Of Trustees Of The Leleand Stanford Junior University High resolution STR analysis using next generation sequencing
GB201515557D0 (en) 2015-09-02 2015-10-14 14M Genomics Ltd Method of sequencing
CN108350491A (en) 2015-11-18 2018-07-31 加利福尼亚太平洋生物科学股份有限公司 Nucleic acid is loaded on base material
JP6975507B2 (en) 2015-12-08 2021-12-01 ツインストランド・バイオサイエンシズ・インコーポレイテッドTwinstrand Biosciences, Inc. Improved adapters, methods, and compositions for double-stranded sequencing
US20170283864A1 (en) * 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
US11821028B2 (en) 2016-07-12 2023-11-21 QIAGEN Sciences, LLP Single end duplex DNA sequencing
WO2018031588A1 (en) 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
CN106367485B (en) 2016-08-29 2019-04-26 厦门艾德生物医药科技股份有限公司 Double label connector groups of a kind of more positioning for detecting gene mutation and its preparation method and application
WO2018175997A1 (en) 2017-03-23 2018-09-27 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
JP7420388B2 (en) 2018-02-13 2024-01-23 ツインストランド・バイオサイエンシズ・インコーポレイテッド Methods and reagents for detecting and evaluating genotoxicity
JP2021515579A (en) 2018-03-15 2021-06-24 ツインストランド・バイオサイエンシズ・インコーポレイテッドTwinstrand Biosciences, Inc. Methods and Reagents for Concentrating Nucleic Acid Substances for Sequencing Applications and Other Nucleic Acid Substance Interrogation
WO2019183640A1 (en) 2018-03-23 2019-09-26 Board Of Regents, The University Of Texas System Efficient sequencing of dsdna with extremely low level of errors
US20210292836A1 (en) 2018-05-16 2021-09-23 Twinstrand Biosciences, Inc. Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications
BR112021000409A2 (en) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. METHODS AND REAGENTS TO CHARACTERIZE GENOMIC EDITION, CLONAL EXPANSION AND ASSOCIATED APPLICATIONS
EP4114978A4 (en) 2020-03-06 2024-07-03 Singular Genomics Systems, Inc. Linked paired strand sequencing

Also Published As

Publication number Publication date
ES2977793T3 (en) 2024-08-30
AU2018366213A1 (en) 2020-05-14
EP3707273A1 (en) 2020-09-16
CA3081027A1 (en) 2019-05-16
EP3707273A4 (en) 2021-09-29
EP4410993A2 (en) 2024-08-07
US20200362390A1 (en) 2020-11-19
WO2019094651A1 (en) 2019-05-16
US11739367B2 (en) 2023-08-29
AU2018366213B2 (en) 2025-05-15
EP3707273B1 (en) 2024-04-10
EP3707273C0 (en) 2024-04-10
EP4410993A3 (en) 2025-02-19
US20240076716A1 (en) 2024-03-07

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