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SG11201809927XA - Improvements in or relating to nucleic acid amplification processes - Google Patents

Improvements in or relating to nucleic acid amplification processes

Info

Publication number
SG11201809927XA
SG11201809927XA SG11201809927XA SG11201809927XA SG11201809927XA SG 11201809927X A SG11201809927X A SG 11201809927XA SG 11201809927X A SG11201809927X A SG 11201809927XA SG 11201809927X A SG11201809927X A SG 11201809927XA SG 11201809927X A SG11201809927X A SG 11201809927XA
Authority
SG
Singapore
Prior art keywords
london
international
nucleic acid
target
lumiradx
Prior art date
Application number
SG11201809927XA
Inventor
Jarrod Provins
Daiwei Shen
Bryan Kraynack
Original Assignee
Lumiradx Uk Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56891177&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG11201809927X(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lumiradx Uk Ltd filed Critical Lumiradx Uk Ltd
Publication of SG11201809927XA publication Critical patent/SG11201809927XA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/307—Single strand endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property :::` , MD 1101111 0 DOI HIM 011101 0 101111111111111111111111111111111111111 0111111 Organization International Bureau (10) International Publication Number 03 (43) International Publication Date .....•\"\" WO 2018/002649 Al 04 January 2018 (04.01.2018) W I PO I PCT (51) International Patent Classification: (GB). SHEN, Daiwei; c/o LumiraDx UK Ltd, 3 More Lon- C12Q 1/68 (2006.01) BOIL 7/00 (2006.01) don Riverside, London Greater London SE1 2AQ (GB). KRAYNACK, Bryan; c/o LumiraDx UK Ltd, 3 More Lon- (21) International Application Number: PCT/GB2017/051927 don Riverside, London Greater London SE1 2AQ (GB). (22) International Filing Date: (74) Agent: LIPSCOMBE, Martin John et al.; Nash Matthews 30 June 2017 (30.06.2017) LLP, 24 Hills Road, Cambridge Cambridgeshire CB2 1JP (GB). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection available): AE, AG, AL, AM, (30) Priority Data: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 1611469.6 30 June 2016 (30.06.2016) GB CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (71) Applicant: LUMIRADX UK LTD [GB/GB]; 3 More Lon- HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, don Riverside, London Greater London SE1 2AQ (GB). KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (72) Inventors: PROVINS, Jarrod; c/o LumiraDx UK Ltd, 3 OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, More London Riverside, London Greater London SE1 2AQ (54) : steps of: a duplex structure by; (b) causing a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend so as and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised (57) 71' hybridisation C temperature Title: IMPROVEMENTS IN OR RELATING TO NUCLEIC ACID AMPLIFICATION PROCESSES comprising permit a the formation amplification the nicked steps in that the amplification = = Average STAR -0.5C Rate, Chlamydia trachomatis gDNA 55000 — = 45000 —:: 8 . 2.3 35000 —:: 13 .. 8 . 5.-z 25000 —:: x, - 60 ______ ------- ----------------- 55 — = = 50 = • ••••• • lt Temperature 'Z.) • • .\" il. , :. .— 40 . . : - E : 35 ' _ • : 30 • — 25 = Target 100 copies M=18) Target 10 copies (n=17) —No Target Control (n=12) = _ = F, \" s • • 2 15000 3 —,:•:•:•:. . D7 5000 —i: ... . . .. _ = _ = = — -5000 —** 0 ----- • - ••••••••.•-• -• . . .....,.... ... — ..............„.........................„................••.................... 4 5 6 7 8 9 10 20 Time (minutes) _ = at FIG. 8 Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method (a) mixing a target sequence with one or more complementary single stranded primers in conditions which event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating which the method is performed is non-isothermal, and subject to a reduction of at least 2° C during the ,-, 01 the ei of © process --..... strands * 1-1 1:) (b) ei C [Continued on next page] WO 2018/002649 Al 0 111 SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with amended claims (Art. 19(1)) process of steps (b)-(d).
SG11201809927XA 2016-06-30 2017-06-30 Improvements in or relating to nucleic acid amplification processes SG11201809927XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1611469.6A GB201611469D0 (en) 2016-06-30 2016-06-30 Improvements in or relating to nucleic acid amplification processes
PCT/GB2017/051927 WO2018002649A1 (en) 2016-06-30 2017-06-30 Improvements in or relating to nucleic acid amplification processes

Publications (1)

Publication Number Publication Date
SG11201809927XA true SG11201809927XA (en) 2019-01-30

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SG11201809927XA SG11201809927XA (en) 2016-06-30 2017-06-30 Improvements in or relating to nucleic acid amplification processes

Country Status (15)

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US (2) US11591643B2 (en)
EP (1) EP3478853B2 (en)
JP (2) JP6957527B2 (en)
KR (1) KR102392010B1 (en)
CN (1) CN109642251B (en)
AU (1) AU2017287852B2 (en)
CA (1) CA3024295C (en)
ES (1) ES2841624T3 (en)
GB (1) GB201611469D0 (en)
IL (1) IL263870A (en)
MX (1) MX2018015421A (en)
RU (1) RU2760915C2 (en)
SG (1) SG11201809927XA (en)
WO (1) WO2018002649A1 (en)
ZA (1) ZA201807460B (en)

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JP6957527B2 (en) 2021-11-02
US20230340579A1 (en) 2023-10-26
RU2019101504A (en) 2020-07-30
CA3024295A1 (en) 2018-01-04
KR20190024890A (en) 2019-03-08
EP3478853B2 (en) 2025-11-26
RU2760915C2 (en) 2021-12-01
US20190226015A1 (en) 2019-07-25
JP2019519231A (en) 2019-07-11
AU2017287852A1 (en) 2018-11-22
US11591643B2 (en) 2023-02-28
ES2841624T3 (en) 2021-07-08
WO2018002649A1 (en) 2018-01-04
GB201611469D0 (en) 2016-08-17
KR102392010B1 (en) 2022-04-27
CA3024295C (en) 2024-09-24
JP2022017268A (en) 2022-01-25
EP3478853A1 (en) 2019-05-08
EP3478853B1 (en) 2020-10-07
ZA201807460B (en) 2021-06-30
RU2019101504A3 (en) 2020-11-30
CN109642251A (en) 2019-04-16
CN109642251B (en) 2022-08-19
BR112018075801A2 (en) 2019-03-26
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MX2018015421A (en) 2019-11-28
IL263870A (en) 2019-01-31

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