SG11201809406PA - Construct and vector for intragenic plant transformation - Google Patents
Construct and vector for intragenic plant transformationInfo
- Publication number
- SG11201809406PA SG11201809406PA SG11201809406PA SG11201809406PA SG11201809406PA SG 11201809406P A SG11201809406P A SG 11201809406PA SG 11201809406P A SG11201809406P A SG 11201809406PA SG 11201809406P A SG11201809406P A SG 11201809406PA SG 11201809406P A SG11201809406P A SG 11201809406PA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- queensland
- plants
- pct
- street
- Prior art date
Links
- 230000009466 transformation Effects 0.000 title abstract 2
- 230000002068 genetic effect Effects 0.000 abstract 3
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000036579 abiotic stress Effects 0.000 abstract 1
- 230000000877 morphologic effect Effects 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 235000019629 palatability Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Virology (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
N O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 02 November 2017 (02.11.2017) WIP0 I PCT ill 111111110111010100111 Hot omolimm minion oimIE (10) International Publication Number WO 2017/185136 Al (51) International Patent Classification: C12N 15/09 (2006.01) Cl 2N 15/82 (2006.01) C12N 15/63 (2006.01) (21) International Application Number: PCT/AU2017/050383 (22) International Filing Date: 27 April 2017 (27.04.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 2016901547 27 April 2016 (27.04.2016) AU (71) Applicant: NEXGEN PLANTS PTY LTD [AU/AU]; Level 7, General Purpose South Building, The University of Queensland, Brisbane, Queensland 4072 (AU). (72) Inventors: SCHENK, Peer Martin Philipp; 76 Lancelot Street, Tennyson, Queensland 4105 (AU). NOWAK, Eka- terina; 41 Wellington Street, Ormiston, Queensland 4160 (AU). (74) Agent: FISHER ADAMS KELLY CALLINANS; Level 6, 175 Eagle Street, Brisbane, Queensland 4000 (AU). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) (54) Title: CONSTRUCT AND VECTOR FOR INTRAGENIC PLANT TRANSFORMATION Mid SfRbc33C ITCA1 AA ACTAi.3 - 1 CAC 3' 332hp Figure 1 (57) : Genetic constructs are provided at least a fragment of which are insertable into the genetic material of a plant, wherein at least a fragment of the genetic construct comprises, consists essentially of, or consists of one or more nucleotide sequences derived from one or more plants. Also provided is use of the genetic construct for the production of genetically improved plants, and improved plants improved thereby. The improved plants may have desirable disease resistance, abiotic stress tolerance, or nutritional, palatability, or morphological properties.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2016901547A AU2016901547A0 (en) | 2016-04-27 | Construct and vector for intragenic plant transformation | |
| PCT/AU2017/050383 WO2017185136A1 (en) | 2016-04-27 | 2017-04-27 | Construct and vector for intragenic plant transformation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201809406PA true SG11201809406PA (en) | 2018-11-29 |
Family
ID=60161702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201809406PA SG11201809406PA (en) | 2016-04-27 | 2017-04-27 | Construct and vector for intragenic plant transformation |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20190127755A1 (en) |
| EP (1) | EP3448994A4 (en) |
| JP (1) | JP2019515693A (en) |
| KR (1) | KR20180137558A (en) |
| CN (1) | CN109477091A (en) |
| BR (1) | BR112018072154A2 (en) |
| CA (1) | CA3022345A1 (en) |
| IL (1) | IL262585A (en) |
| PH (1) | PH12018502273A1 (en) |
| SG (1) | SG11201809406PA (en) |
| WO (1) | WO2017185136A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3145052A1 (en) * | 2019-07-05 | 2021-01-14 | Bejo Zaden B.V. | Tomato spotted wilt virus resistance in cichorium |
| WO2021119130A1 (en) * | 2019-12-09 | 2021-06-17 | Texas Tech University System | Method and compositions for engineering grapevine red blotch virus-resistant grapevine |
| CN113832181B (en) * | 2021-08-24 | 2023-06-27 | 北大荒垦丰种业股份有限公司 | Gene editing method of japonica rice improved strain aroma control gene and application thereof |
| CN114395570A (en) * | 2021-12-29 | 2022-04-26 | 西南大学 | A method using CsNCED3 gene silencing to improve citrus canker resistance |
| CN116200400B (en) * | 2023-01-06 | 2025-06-06 | 浙江大学 | A method for enhancing resistance of tomatoes to root-knot nematodes |
| CN116590317B (en) * | 2023-06-09 | 2025-06-10 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | A tobacco aroma gene and its targeting sgRNA and application |
| WO2025111662A1 (en) * | 2023-11-29 | 2025-06-05 | Commonwealth Scientific And Industrial Research Organisation | Plant transformation |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6576814B1 (en) * | 1998-07-07 | 2003-06-10 | Pioneer Hi-Bred International, Inc. | Manipulation of Mlo genes to enhance disease resistance in plants |
| NZ585399A (en) * | 2002-02-20 | 2012-07-27 | Simplot Co J R | Recombinant plant comprising the insertion of a native genetic element without the insertion of border sequences or selectable markers |
| US7534934B2 (en) * | 2002-02-20 | 2009-05-19 | J.R. Simplot Company | Precise breeding |
| WO2005121346A1 (en) * | 2004-06-08 | 2005-12-22 | New Zealand Institute For Crop & Food Research | Transformation vectors |
| CA2581440C (en) * | 2004-09-24 | 2016-11-29 | J.R. Simplot Company | Gene silencing |
| BRPI0616091B8 (en) * | 2005-09-20 | 2020-01-28 | Simplot Co J R | product processed by heat and respective production methods of edible plant and transgenic plant product, reduction of acrylamide content in plant product and gene overexpression in potato tuber, isolated polynucleotide and plant sequence |
| CA2749440A1 (en) * | 2009-01-15 | 2011-08-12 | The New Zealand Institute For Plant And Food Research Limited | Plant transformation using dna minicircles |
| IN2014DN02042A (en) * | 2011-10-28 | 2015-05-15 | Du Pont |
-
2017
- 2017-04-27 CN CN201780041349.1A patent/CN109477091A/en active Pending
- 2017-04-27 WO PCT/AU2017/050383 patent/WO2017185136A1/en not_active Ceased
- 2017-04-27 KR KR1020187034257A patent/KR20180137558A/en not_active Withdrawn
- 2017-04-27 SG SG11201809406PA patent/SG11201809406PA/en unknown
- 2017-04-27 JP JP2019507974A patent/JP2019515693A/en not_active Withdrawn
- 2017-04-27 CA CA3022345A patent/CA3022345A1/en not_active Abandoned
- 2017-04-27 BR BR112018072154A patent/BR112018072154A2/en not_active IP Right Cessation
- 2017-04-27 EP EP17788430.1A patent/EP3448994A4/en not_active Withdrawn
-
2018
- 2018-10-24 IL IL262585A patent/IL262585A/en unknown
- 2018-10-25 PH PH12018502273A patent/PH12018502273A1/en unknown
- 2018-10-26 US US16/172,228 patent/US20190127755A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017185136A1 (en) | 2017-11-02 |
| IL262585A (en) | 2018-12-31 |
| US20190127755A1 (en) | 2019-05-02 |
| KR20180137558A (en) | 2018-12-27 |
| JP2019515693A (en) | 2019-06-13 |
| EP3448994A4 (en) | 2019-05-29 |
| BR112018072154A2 (en) | 2019-03-19 |
| PH12018502273A1 (en) | 2019-09-09 |
| CA3022345A1 (en) | 2017-11-02 |
| EP3448994A1 (en) | 2019-03-06 |
| CN109477091A (en) | 2019-03-15 |
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