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SG10201906673WA - Artificial nucleic acid molecules - Google Patents

Artificial nucleic acid molecules

Info

Publication number
SG10201906673WA
SG10201906673WA SG10201906673WA SG10201906673WA SG10201906673WA SG 10201906673W A SG10201906673W A SG 10201906673WA SG 10201906673W A SG10201906673W A SG 10201906673WA SG 10201906673W A SG10201906673W A SG 10201906673WA SG 10201906673W A SG10201906673W A SG 10201906673WA
Authority
SG
Singapore
Prior art keywords
nucleic acid
artificial nucleic
utr
utr element
acid molecules
Prior art date
Application number
SG10201906673WA
Inventor
Stefanie Grund
Thomas Schlake
Original Assignee
Curevac Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Curevac Ag filed Critical Curevac Ag
Publication of SG10201906673WA publication Critical patent/SG10201906673WA/en

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
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    • A61P25/00—Drugs for disorders of the nervous system
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
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    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/10—Vectors comprising a special translation-regulating system regulates levels of translation
    • C12N2840/105—Vectors comprising a special translation-regulating system regulates levels of translation enhancing translation

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  • Health & Medical Sciences (AREA)
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Abstract

ARTIFICIAL NUCLEIC ACID MOLECULES The invention relates to an artificial nucleic acid molecule comprising at least one open reading 5 frame and at least one 3’-untranslated region element (3’-UTR element) and/or at least one 5’- untranslated region element (5’-UTR element), wherein the at least one 3’-UTR element and/or the at least one 5’-UTR element prolongs and/or increases protein production from said artificial nucleic acid molecule and wherein the at least one 3’-UTR element and/or the at least one 5’-UTR element is derived from a stable mRNA. The invention further relates to the use of such an 10 artificial nucleic acid molecule in gene therapy and/or genetic vaccination. Furthermore, methods for identifying a 3’-UTR element and/or a 5’-UTR derived from a stable mRNA element are disclosed. (No suitable figure) 15
SG10201906673WA 2014-12-30 2015-12-29 Artificial nucleic acid molecules SG10201906673WA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP2014003479 2014-12-30

Publications (1)

Publication Number Publication Date
SG10201906673WA true SG10201906673WA (en) 2019-09-27

Family

ID=52814040

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SG10201906673WA SG10201906673WA (en) 2014-12-30 2015-12-29 Artificial nucleic acid molecules
SG11201704681QA SG11201704681QA (en) 2014-12-30 2015-12-29 Artificial nucleic acid molecules

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Application Number Title Priority Date Filing Date
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Country Status (12)

Country Link
US (3) US20180148727A1 (en)
EP (2) EP3494982A1 (en)
JP (2) JP6907116B2 (en)
KR (1) KR102580696B1 (en)
CN (1) CN107124889A (en)
AU (1) AU2015373404B2 (en)
BR (1) BR112017009835A2 (en)
CA (1) CA2966092A1 (en)
MX (1) MX2017008670A (en)
RU (1) RU2757675C2 (en)
SG (2) SG10201906673WA (en)
WO (1) WO2016107877A1 (en)

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