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SG10201810877TA - Method for determining a protein-protein interaction - Google Patents

Method for determining a protein-protein interaction

Info

Publication number
SG10201810877TA
SG10201810877TA SG10201810877TA SG10201810877TA SG10201810877TA SG 10201810877T A SG10201810877T A SG 10201810877TA SG 10201810877T A SG10201810877T A SG 10201810877TA SG 10201810877T A SG10201810877T A SG 10201810877TA SG 10201810877T A SG10201810877T A SG 10201810877TA
Authority
SG
Singapore
Prior art keywords
protein
determining
interaction
cell
fusion
Prior art date
Application number
SG10201810877TA
Inventor
Taku Watanabe
Toru Shoji
Shun MATSUZAWA
Original Assignee
Medical & Biological Laboratories Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical & Biological Laboratories Co Ltd filed Critical Medical & Biological Laboratories Co Ltd
Publication of SG10201810877TA publication Critical patent/SG10201810877TA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

METHOD FOR DETERMINING A PROTEIN-PROTEIN INTERACTION A method for determining an interaction between a 5 first protein and a second protein comprises the steps of: expressing in a cell or introducing into a cell a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, and 10 a second fusion protein comprising the second protein and a multimerizable protein; detecting a fluorescent focus formed by an association between the first fusion protein and the second fusion protein in the cell; and 15 determining an interaction between the first protein and the second protein according to the detection of the fluorescent focus. 20 Figure 1
SG10201810877TA 2014-06-10 2015-06-10 Method for determining a protein-protein interaction SG10201810877TA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2014119441 2014-06-10

Publications (1)

Publication Number Publication Date
SG10201810877TA true SG10201810877TA (en) 2019-01-30

Family

ID=54833618

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201810877TA SG10201810877TA (en) 2014-06-10 2015-06-10 Method for determining a protein-protein interaction
SG11201610359QA SG11201610359QA (en) 2014-06-10 2015-06-10 Method for determining a protein-protein interaction

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201610359QA SG11201610359QA (en) 2014-06-10 2015-06-10 Method for determining a protein-protein interaction

Country Status (6)

Country Link
US (1) US10761085B2 (en)
EP (1) EP3156484A4 (en)
JP (1) JPWO2015190529A1 (en)
CA (1) CA2951630A1 (en)
SG (2) SG10201810877TA (en)
WO (1) WO2015190529A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019515252A (en) * 2016-03-15 2019-06-06 ラボラトリー コーポレイション オブ アメリカ ホールディングス Method of evaluating protein interaction between cells
JPWO2018235787A1 (en) * 2017-06-19 2020-04-16 株式会社医学生物学研究所 Method for determining protein-protein interaction using fluorescent protein
CN114645065A (en) * 2020-12-17 2022-06-21 华东理工大学 Novel photo-chemical small molecule double-induction gene expression system
WO2025065479A1 (en) * 2023-09-28 2025-04-03 Westlake University Chemically programmable systems for controlled protein translocation and gene expression in cell

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
CA2345392A1 (en) 1998-09-24 2000-03-30 Duke University Method of measuring protein-protein interactions in living cells
JP2001327296A (en) * 2000-03-15 2001-11-27 Japan Science & Technology Corp Method for detecting protein-protein interaction
CA2414626A1 (en) 2000-07-04 2002-01-10 Bioimage A/S A method for extracting quantitative information relating to interactions between cellular components
EP1349569B1 (en) 2001-01-12 2007-04-18 Becton Dickinson and Company Intrinsically fluorescent, self-multimerizing mhc fusion proteins and complexes thereof
US20090221440A1 (en) * 2004-07-12 2009-09-03 Board Of Regents, The University Of Texas System Methods and compositions related to identifying protein-protein interactions
WO2006062882A2 (en) 2004-12-04 2006-06-15 The Regents Of The University Of California Los Alamos National Laboratory Protein-protein interaction detection system using fluorescent protein microdomains
WO2006099486A2 (en) 2005-03-15 2006-09-21 President And Fellows Of Harvard College Method and compounds for detecting protein-protein and protein-nucleic acid interactions
JP5019771B2 (en) * 2005-03-29 2012-09-05 独立行政法人理化学研究所 Analysis method of protein interaction using fluorescent protein
WO2009025475A2 (en) 2007-08-17 2009-02-26 Korea Advanced Institute Of Science And Technology The methods for detecting molecular interactions
WO2009049892A1 (en) * 2007-10-17 2009-04-23 European Molecular Biology Laboratory (Embl) Polypeptides comprising an annexin core domain, compositions, methods and use thereof
ES2606706T3 (en) * 2008-01-09 2017-03-27 Ludwig-Maximilians-Universitat München A double fluorescent hybrid (F2H) assay for direct visualization of protein interactions in living cells
JP5476627B2 (en) * 2008-10-20 2014-04-23 学校法人東京農業大学 Cyclin A-p27 interaction detection method
WO2010066113A1 (en) * 2008-12-11 2010-06-17 国立大学法人东京大学 Composition of fusion proteins of split fluorescent proteins, expression vector, expression stable cell line, and screening method thereof
JP2011211983A (en) * 2010-03-31 2011-10-27 Osaka Prefecture Univ Protein molecule pair, gene encoding protein molecule pair, and cell producing gene transfer vector and protein molecule pair
US8877446B2 (en) * 2011-08-26 2014-11-04 Korea Research Institute Of Bioscience And Biotechnology Method for detecting protein-protein interactions in cells
KR102051159B1 (en) * 2011-12-05 2019-12-02 가부시키가이샤 이가쿠세이부츠가쿠겐큐쇼 Method for detecting protein-protein interaction

Also Published As

Publication number Publication date
WO2015190529A1 (en) 2015-12-17
US20170122932A1 (en) 2017-05-04
JPWO2015190529A1 (en) 2017-04-20
US10761085B2 (en) 2020-09-01
EP3156484A4 (en) 2017-12-27
SG11201610359QA (en) 2017-01-27
EP3156484A1 (en) 2017-04-19
CA2951630A1 (en) 2015-12-17

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