SG10201808952WA - Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications - Google Patents
Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applicationsInfo
- Publication number
- SG10201808952WA SG10201808952WA SG10201808952WA SG10201808952WA SG10201808952WA SG 10201808952W A SG10201808952W A SG 10201808952WA SG 10201808952W A SG10201808952W A SG 10201808952WA SG 10201808952W A SG10201808952W A SG 10201808952WA SG 10201808952W A SG10201808952W A SG 10201808952WA
- Authority
- SG
- Singapore
- Prior art keywords
- nucleic acid
- amplification
- replication
- genomic
- detection
- Prior art date
Links
- 102000039446 nucleic acids Human genes 0.000 title abstract 8
- 108020004707 nucleic acids Proteins 0.000 title abstract 8
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 230000010076 replication Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 230000001225 therapeutic effect Effects 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 abstract 6
- 208000036878 aneuploidy Diseases 0.000 abstract 2
- 231100001075 aneuploidy Toxicity 0.000 abstract 2
- 102000054766 genetic haplotypes Human genes 0.000 abstract 2
- 230000003993 interaction Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000005096 rolling process Methods 0.000 abstract 2
- 238000012163 sequencing technique Methods 0.000 abstract 2
- 239000012190 activator Substances 0.000 abstract 1
- 238000003491 array Methods 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000002759 chromosomal effect Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 150000003384 small molecules Chemical class 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/507—Recombinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/519—Topoisomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
- C12Q2522/10—Nucleic acid binding proteins
- C12Q2522/101—Single or double stranded nucleic acid binding proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
SYSTEMSAND METHODS FOR CLONAL REPLICATION AND AMPLIFICATION OF NUCLEIC ACID MOLECULES FOR GENOMIC AND THERAPEUTIC APPLICATIONS The present invention provides for methods, reagents, apparatuses, and systems for the replication or amplification of nucleic acid molecules from biological samples. In one embodiment of the invention, the nucleic molecules are isolated from the sample, and subjected to fragmenting and joining using ligating agents of one or more hairpin structures to each end of the fragmented nucleic molecules to form one or more dumbbell templates. The one or more dumbbell templates are contacted with at least one substantially complementary primer attached to a substrate, and subjected to rolling circle replication or rolling circle amplification. The resulting replicated dumbbell templates or amplified dumbbell templates are used in numerous genomic applications, including whole genome de novo sequencing; sequence variant detection, structural variant detection, determining the phase of molecular haplotypes, molecular counting for aneuploidy detection; targeted sequencing of gene panels, whole exome, or chromosomal regions for sequence variant detection, structural variant detection, determining the phase of molecular haplotypes and/or molecular counting for aneuploidy detection; study of nucleic acid - nucleic acid binding interactions, nucleic acid - protein binding interactions, and nucleic acid molecule expression arrays; and testing of the effects of small molecule inhibitors or activators or nucleic acid therapeutics. No suitable figure
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461978823P | 2014-04-11 | 2014-04-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201808952WA true SG10201808952WA (en) | 2018-11-29 |
Family
ID=54288469
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201808952WA SG10201808952WA (en) | 2014-04-11 | 2015-04-11 | Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications |
| SG11201608486SA SG11201608486SA (en) | 2014-04-11 | 2015-04-11 | Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201608486SA SG11201608486SA (en) | 2014-04-11 | 2015-04-11 | Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20170067097A1 (en) |
| EP (1) | EP3129505B1 (en) |
| JP (2) | JP7058502B2 (en) |
| KR (1) | KR102592367B1 (en) |
| CN (2) | CN113528628A (en) |
| AU (1) | AU2015243130B2 (en) |
| CA (1) | CA2945358C (en) |
| DK (1) | DK3129505T3 (en) |
| SG (2) | SG10201808952WA (en) |
| WO (1) | WO2015157747A1 (en) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018081666A1 (en) * | 2016-10-28 | 2018-05-03 | Silgentech Inc. | Methods of single dna/rna molecule counting |
| EP3714064B8 (en) * | 2017-11-21 | 2024-10-30 | 4basebio UK Ltd | Methods and kits for amplification of double stranded dna |
| CN111936635B (en) * | 2018-03-02 | 2024-08-23 | 豪夫迈·罗氏有限公司 | Generation of single-stranded circular DNA templates for single-molecule sequencing |
| KR102131494B1 (en) * | 2018-04-17 | 2020-07-07 | 건국대학교 산학협력단 | Detection of Target Nucleic Acid Sequences by PCR-coupled Rolling Circle Amplification |
| CN109055488B (en) * | 2018-08-17 | 2021-11-02 | 中山康源基因技术科技有限公司 | Method for preparing long probe by using annular single-chain probe and application of long probe in gene chip production |
| EP3650559B1 (en) | 2018-11-08 | 2022-06-29 | Siemens Healthcare GmbH | Direct rna nanopore sequencing with help of a stem-loop polynucleotide |
| SG11202105957YA (en) * | 2018-12-05 | 2021-07-29 | Egi Tech Shen Zhen Co Limited | Rolling circle amplification method, method for preparing sequencing library, and dna nanosphere prepared therefrom |
| CN111378750A (en) * | 2018-12-29 | 2020-07-07 | 北京福安华生物科技有限公司 | Artificial mimic nucleic acid molecular beacon and kit for detecting rs885004 site polymorphism of SLC28A3 gene |
| GB201911421D0 (en) * | 2019-08-09 | 2019-09-25 | Dnae Diagnostics Ltd | Methods of multiplexed isothermal amplification of nucleic acid sequences |
| SG11202110571YA (en) | 2019-10-25 | 2021-10-28 | Illumina Cambridge Ltd | Methods for generating, and sequencing from, asymmetric adaptors on the ends of polynucleotide templates comprising hairpin loops |
| CN111139289A (en) * | 2019-12-25 | 2020-05-12 | 中国海洋大学 | Constant temperature rolling circle amplification method based on dumbbell circle template |
| US20230323343A1 (en) * | 2020-01-31 | 2023-10-12 | Tyris Therapeutics, S.L. | Closed linear dna with modified nucleotides |
| EP4150126A4 (en) * | 2020-05-12 | 2024-06-19 | Singular Genomics Systems, Inc. | METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS |
| EP4153606A4 (en) | 2020-07-13 | 2024-10-02 | Singular Genomics Systems, Inc. | Methods of sequencing complementary polynucleotides |
| WO2022141061A1 (en) * | 2020-12-29 | 2022-07-07 | 深圳华大智造科技股份有限公司 | Method for rapidly constructing cyclized library and ring-forming linker |
| CA3233230A1 (en) * | 2021-09-27 | 2023-03-30 | Jin Huh | Synthetic production of circular dna vectors |
| IL322624A (en) * | 2023-02-23 | 2025-10-01 | Centre For Novostics | Single-molecule strand-specific end modalities |
| FI20245658A1 (en) * | 2024-05-23 | 2025-11-24 | Genomill Health Oy | Method of amplification and storage of circular nucleic acid molecules |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7244559B2 (en) * | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| JP2003532380A (en) * | 1999-12-02 | 2003-11-05 | モレキュラー ステージング，インコーポレイテッド | Production of single-stranded circular DNA from linear self-annealing segments |
| US7452699B2 (en) * | 2003-01-15 | 2008-11-18 | Dana-Farber Cancer Institute, Inc. | Amplification of DNA in a hairpin structure, and applications |
| WO2005014810A1 (en) * | 2003-08-08 | 2005-02-17 | National Institute Of Advanced Industrial Science And Technology | Efficient process for producing dumbbell dna |
| US20080021205A1 (en) * | 2003-12-11 | 2008-01-24 | Helen Blau | Methods and Compositions for Use in Preparing Hairpin Rnas |
| GB2413796B (en) * | 2004-03-25 | 2006-03-29 | Global Genomics Ab | Methods and means for nucleic acid sequencing |
| WO2006071582A2 (en) * | 2004-12-29 | 2006-07-06 | Applera Corporation | Methods, compositions, and kits for forming self-complementary polynucleotides |
| EP1885880B1 (en) * | 2005-04-29 | 2010-07-21 | Synthetic Genomics, Inc. | Amplification and cloning of single dna molecules using rolling circle amplification |
| KR101414713B1 (en) * | 2007-10-11 | 2014-07-03 | 삼성전자주식회사 | Methods for amplifying a target nucleic acid by rolling circle amplification in the presence of ligase and endonuclease |
| BRPI0909212A2 (en) * | 2008-03-28 | 2015-08-18 | Pacific Biosciences California | Compositions and method for nucleic acid sequencing |
| EP2327782B1 (en) * | 2008-09-04 | 2015-02-25 | Biotechnology Research Institute Chinese Academy of Agricultural Sciences | Dna molecule for expressing haripin rna, the constructing method and the use thereof |
| GB0906130D0 (en) * | 2008-10-03 | 2009-05-20 | Procrata Biosystems Ltd | Transcription factor decoys |
| US9267168B2 (en) * | 2012-06-12 | 2016-02-23 | Pacific Biosciences Of California, Inc. | Methods and compositions for isolating template nucleic acids |
| ES2749463T3 (en) * | 2012-11-02 | 2020-03-20 | Life Technologies Corp | Capture, detection and quantification of small RNA |
-
2015
- 2015-04-11 EP EP15776462.2A patent/EP3129505B1/en active Active
- 2015-04-11 CN CN202110947582.4A patent/CN113528628A/en active Pending
- 2015-04-11 CA CA2945358A patent/CA2945358C/en active Active
- 2015-04-11 WO PCT/US2015/025492 patent/WO2015157747A1/en not_active Ceased
- 2015-04-11 AU AU2015243130A patent/AU2015243130B2/en not_active Ceased
- 2015-04-11 JP JP2017504631A patent/JP7058502B2/en active Active
- 2015-04-11 US US15/122,543 patent/US20170067097A1/en not_active Abandoned
- 2015-04-11 SG SG10201808952WA patent/SG10201808952WA/en unknown
- 2015-04-11 SG SG11201608486SA patent/SG11201608486SA/en unknown
- 2015-04-11 CN CN201580030686.1A patent/CN106460065A/en active Pending
- 2015-04-11 DK DK15776462.2T patent/DK3129505T3/en active
- 2015-04-11 KR KR1020167031675A patent/KR102592367B1/en active Active
-
2020
- 2020-06-02 JP JP2020096090A patent/JP7100680B2/en active Active
-
2024
- 2024-07-08 US US18/766,601 patent/US20240368682A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN106460065A (en) | 2017-02-22 |
| WO2015157747A9 (en) | 2016-08-25 |
| JP7100680B2 (en) | 2022-07-13 |
| KR102592367B1 (en) | 2023-10-26 |
| CA2945358A1 (en) | 2015-10-15 |
| AU2015243130B2 (en) | 2021-08-26 |
| AU2015243130A1 (en) | 2016-11-03 |
| EP3129505A1 (en) | 2017-02-15 |
| JP2017512071A (en) | 2017-05-18 |
| US20240368682A1 (en) | 2024-11-07 |
| US20170067097A1 (en) | 2017-03-09 |
| EP3129505A4 (en) | 2017-12-06 |
| CN113528628A (en) | 2021-10-22 |
| CA2945358C (en) | 2022-12-06 |
| KR20160138579A (en) | 2016-12-05 |
| EP3129505B1 (en) | 2021-06-30 |
| JP2020188774A (en) | 2020-11-26 |
| SG11201608486SA (en) | 2016-11-29 |
| JP7058502B2 (en) | 2022-04-22 |
| DK3129505T3 (en) | 2021-10-04 |
| WO2015157747A1 (en) | 2015-10-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SG10201808952WA (en) | Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications | |
| Tee et al. | Erk1/2 activity promotes chromatin features and RNAPII phosphorylation at developmental promoters in mouse ESCs | |
| MY205579A (en) | Nuclease-based rna depletion | |
| Rashid et al. | Contemporary molecular tools in microbial ecology and their application to advancing biotechnology | |
| NZ723570A (en) | Compositions and methods for quantifying a nucleic acid sequence in a sample | |
| BR112015023108A8 (en) | bacterial cell packaging system, method for packaging a reporter nucleic acid molecule, composition, method for detecting a bacterial cell presence or absence in a sample, vector, method for detecting a target transcript in a cell, method for detect the presence of a bacterial cell in a sample and kit. | |
| AU2017257624A1 (en) | Hybridization chain reaction methods for in situ molecular detection | |
| SA516371691B1 (en) | Methods and compositions for dna profiling | |
| AU2018234832A1 (en) | CRISPR effector system based diagnostics for virus detection | |
| WO2015160895A3 (en) | Modified transposases for improved insertion sequence bias and increased dna input tolerance | |
| WO2015081229A3 (en) | Selective amplification of nucleic acid sequences | |
| GB2470672A (en) | Methods of RNA aplification in the presence of DNA | |
| GB201319347D0 (en) | Compositions and kits for molecular counting | |
| ATE541946T1 (en) | METHOD AND SYSTEM FOR DETERMINING GENE COPY NUMBER VARIANTS | |
| BR112017008082A2 (en) | compositions and methods for detecting an rna virus | |
| EP4219739A3 (en) | Polymerase compositions, methods of making and using same | |
| WO2014052487A8 (en) | Two-primer pcr for microrna multiplex assay | |
| JOP20220004A1 (en) | Polynucleotides for the amplification and detection of Neisseria gonorrhoeae | |
| GB2542576A (en) | Method and kit of detecting the absence of micro-oranisms | |
| WO2016059474A3 (en) | Sequence conversion and signal amplifier dna having poly dna spacer sequences and detection methods using same | |
| GB2515944A (en) | Peptide arrays | |
| MX377788B (en) | COMPOSITIONS AND METHODS FOR THE DETECTION AND ANALYSIS OF MYCOBACTERIAL TUBERCULOSIS. | |
| SG10201906653YA (en) | Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level | |
| WO2007014305A3 (en) | Assays for resistance to echinocandin-class drugs | |
| MX394156B (en) | SUBSTRATE MOLECULE. |