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SG10201807572PA - Robust antibody purification - Google Patents

Robust antibody purification

Info

Publication number
SG10201807572PA
SG10201807572PA SG10201807572PA SG10201807572PA SG10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA SG 10201807572P A SG10201807572P A SG 10201807572PA
Authority
SG
Singapore
Prior art keywords
antibody purification
robust antibody
robust
hcps
separation
Prior art date
Application number
SG10201807572PA
Inventor
Romas Skudas
Matthias Joehnck
Bianca Edelmann
Simon Braun
Mikhail Kozlov
Matthew Stone
Kevin Galipeau
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of SG10201807572PA publication Critical patent/SG10201807572PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

ROBUST ANTIBODY PURIFICATION The present invention refers to a method for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. (No suitable figure)
SG10201807572PA 2014-03-04 2015-02-18 Robust antibody purification SG10201807572PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201461947761P 2014-03-04 2014-03-04

Publications (1)

Publication Number Publication Date
SG10201807572PA true SG10201807572PA (en) 2018-10-30

Family

ID=52589333

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201607341SA SG11201607341SA (en) 2014-03-04 2015-02-18 Robust antibody purification
SG10201807572PA SG10201807572PA (en) 2014-03-04 2015-02-18 Robust antibody purification

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201607341SA SG11201607341SA (en) 2014-03-04 2015-02-18 Robust antibody purification

Country Status (10)

Country Link
US (1) US10457720B2 (en)
EP (1) EP3114455B1 (en)
JP (2) JP7037275B2 (en)
KR (1) KR102405382B1 (en)
CN (1) CN106103477B (en)
CA (1) CA2942134C (en)
DK (1) DK3114455T3 (en)
ES (1) ES2784638T3 (en)
SG (2) SG11201607341SA (en)
WO (1) WO2015131978A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3233230B1 (en) 2014-12-15 2024-06-12 Merck Patent GmbH Target molecule capture from crude solutions
MX2018006789A (en) 2015-12-03 2019-02-13 Juno Therapeutics Inc MODIFIED CHEMICAL RECEIVERS AND RELATED COMPOSITIONS AND METHODS.
US11186858B1 (en) 2016-03-15 2021-11-30 Fresenius Kabi Deutschland Gmbh Methods for increasing biosimilarity
CN109715648A (en) * 2016-09-15 2019-05-03 克拉维格两合股份有限公司 Polymeric mesh object is used for the purposes of macromolecule purifying
CN106674403A (en) * 2017-01-03 2017-05-17 宏葵生物（中国）有限公司 Preparation method of polystyrene microspheres for enhancing immunoturbidimetry and application thereof
WO2019164227A1 (en) * 2018-02-20 2019-08-29 고려대학교 산학협력단 Multi-column for isolating exosomes and exosome isolation method
JP7404689B2 (en) * 2018-08-06 2023-12-26 東ソー株式会社 Antibody adsorbent with immobilized Fc-binding protein and antibody separation method using the same
CN117241881A (en) * 2021-06-10 2023-12-15 三菱化学株式会社 Synthetic adsorbent, antibody purification method and antibody manufacturing method
IL310068A (en) * 2021-07-29 2024-03-01 Csl Behring Ag Method of purifying immunoglobulin g and uses thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4382124B1 (en) 1958-07-18 1994-10-04 Rohm & Haas Process for preparing macroreticular resins, copolymers and products of said process
JPS5798504A (en) 1980-12-11 1982-06-18 Mitsubishi Monsanto Chem Co Production of vinyl polymer
JP2534979B2 (en) 1984-12-27 1996-09-18 東ソー株式会社 Filler for liquid chromatography
JPS61141704A (en) 1985-11-11 1986-06-28 Mitsubishi Kasei Vinyl Co Production of vinyl polymer
US6251996B1 (en) * 1998-12-09 2001-06-26 The Dow Chemical Company Gel-type copolymer bead and ion-exchange resins made therefrom
EP1179732B2 (en) 2000-08-11 2012-02-29 Rohm And Haas Company Polymeric adsorbent and method of preparation
HUE025101T2 (en) 2002-04-26 2016-02-29 Genentech Inc Non-affinity purification of proteins
US7427659B2 (en) * 2003-10-24 2008-09-23 Amgen Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
CN1927899A (en) * 2006-09-01 2007-03-14 烟台硕德新材料有限公司 Preparation method of micron-sized monodisperse copolymerized microspheres
KR20120118065A (en) * 2010-02-12 2012-10-25 디에스엠 아이피 어셋츠 비.브이. Single unit antibody purification
JP6292718B2 (en) * 2011-07-01 2018-03-14 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Method for separating monomeric polypeptides from aggregated polypeptides
WO2013028330A2 (en) * 2011-08-19 2013-02-28 Emd Millipore Corporation Methods of reducing level of one of more impurities in a sample during protein purification
EP2970378B1 (en) * 2013-03-15 2021-05-26 Biogen MA Inc. Hydrophobic interaction protein chromatography under no-salt conditions
US8946395B1 (en) * 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
EP3233230B1 (en) * 2014-12-15 2024-06-12 Merck Patent GmbH Target molecule capture from crude solutions

Also Published As

Publication number Publication date
JP2017509621A (en) 2017-04-06
US20170073394A1 (en) 2017-03-16
US10457720B2 (en) 2019-10-29
KR20160128411A (en) 2016-11-07
CA2942134C (en) 2023-08-15
SG11201607341SA (en) 2016-10-28
JP7037275B2 (en) 2022-03-16
ES2784638T3 (en) 2020-09-29
CN106103477B (en) 2021-08-17
CA2942134A1 (en) 2015-09-11
WO2015131978A1 (en) 2015-09-11
EP3114455B1 (en) 2020-01-15
KR102405382B1 (en) 2022-06-03
JP2020128373A (en) 2020-08-27
JP7153041B2 (en) 2022-10-13
DK3114455T3 (en) 2020-04-06
CN106103477A (en) 2016-11-09
EP3114455A1 (en) 2017-01-11

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