SG10201806697RA - Method for detecting basophil activation - Google Patents
Method for detecting basophil activationInfo
- Publication number
- SG10201806697RA SG10201806697RA SG10201806697RA SG10201806697RA SG10201806697RA SG 10201806697R A SG10201806697R A SG 10201806697RA SG 10201806697R A SG10201806697R A SG 10201806697RA SG 10201806697R A SG10201806697R A SG 10201806697RA SG 10201806697R A SG10201806697R A SG 10201806697RA
- Authority
- SG
- Singapore
- Prior art keywords
- basophils
- allergen
- agent
- ligand
- activation
- Prior art date
Links
- 210000003651 basophil Anatomy 0.000 title abstract 11
- 230000004913 activation Effects 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 239000013566 allergen Substances 0.000 abstract 4
- 239000003795 chemical substances by application Substances 0.000 abstract 4
- 239000003446 ligand Substances 0.000 abstract 4
- 210000004369 blood Anatomy 0.000 abstract 3
- 239000008280 blood Substances 0.000 abstract 3
- 239000003550 marker Substances 0.000 abstract 3
- 239000007864 aqueous solution Substances 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- GRYSXUXXBDSYRT-WOUKDFQISA-N (2r,3r,4r,5r)-2-(hydroxymethyl)-4-methoxy-5-[6-(methylamino)purin-9-yl]oxolan-3-ol Chemical compound C1=NC=2C(NC)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1OC GRYSXUXXBDSYRT-WOUKDFQISA-N 0.000 abstract 1
- 206010020751 Hypersensitivity Diseases 0.000 abstract 1
- 208000026935 allergic disease Diseases 0.000 abstract 1
- 230000007815 allergy Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000003743 erythrocyte Anatomy 0.000 abstract 1
- 230000002934 lysing effect Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
- G01N33/6869—Interleukin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/41—Detecting, measuring or recording for evaluating the immune or lymphatic systems
- A61B5/411—Detecting or monitoring allergy or intolerance reactions to an allergenic agent or substance
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5047—Cells of the immune system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/24—Immunology or allergic disorders
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plasma & Fusion (AREA)
- Ecology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Physiology (AREA)
- Zoology (AREA)
- Virology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Vascular Medicine (AREA)
- Medical Informatics (AREA)
- Heart & Thoracic Surgery (AREA)
- Biophysics (AREA)
- Rehabilitation Therapy (AREA)
- Rheumatology (AREA)
Abstract
METHODFOR DETECTING BASOPHIL ACTIVATION The present invention relates to a method for diagnosing an allergy, comprising the steps of bringing into contact of basophils from a whole blood sample of a patient with an allergen in an aqueous solution in the presence of whole blood, with a volume content of whole blood in the aqueous solution of at least percent by volume, under conditions that allow activation of the basophils by the allergen, enrichment of the basophils from step a) and detection of activated basophils, wherein the detection is carried out in that the expression of a marker characteristic of activated basophils is detected by means of chemiluminescence; and a kit comprising a ves- sel for bringing the basophils into contact with the allergen, wherein the vessel is preferably a microtiter plate, optionally an allergen, an agent for detecting a marker characteristic of activat- ed basophils by means of chemiluminescence, wherein the agent is preferably a ligand against the marker provided with a label capable of chemiluminescence and the ligand more preferably is a monoclonal antibody, optionally an agent for stopping activation of the basophils, an option- ally immobilizable ligand that binds to a polypeptide located on the cell surface of basophils in- dependently of its activation state, wherein the ligand is preferably a monoclonal antibody against the polypeptide, and optionally an agent for lysing and/or removing erythrocytes. [No suitable figure] 35
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17001352.8A EP3441763A1 (en) | 2017-08-08 | 2017-08-08 | Method for detecting basophil activation |
| EP17001979 | 2017-12-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201806697RA true SG10201806697RA (en) | 2019-03-28 |
Family
ID=63041937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201806697RA SG10201806697RA (en) | 2017-08-08 | 2018-08-06 | Method for detecting basophil activation |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US11119109B2 (en) |
| EP (1) | EP3441765B1 (en) |
| JP (1) | JP7265325B2 (en) |
| KR (1) | KR102547565B1 (en) |
| CN (1) | CN109387636B (en) |
| AU (1) | AU2018213977B2 (en) |
| BR (1) | BR102018015288A2 (en) |
| CA (1) | CA3012989A1 (en) |
| DE (1) | DE102018006212A1 (en) |
| ES (1) | ES2904354T3 (en) |
| LT (1) | LT3441765T (en) |
| PL (1) | PL3441765T3 (en) |
| SG (1) | SG10201806697RA (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210382070A1 (en) * | 2020-06-04 | 2021-12-09 | The Chinese University Of Hong Kong | Diagnostic biomarkers for shrimp allergy |
| CN112630423A (en) * | 2020-12-31 | 2021-04-09 | 杭州福德敏生物技术有限公司 | Wheat immunofluorescence detection test strip and application |
| KR102497904B1 (en) * | 2021-02-10 | 2023-02-10 | 재단법인 아산사회복지재단 | Method for detecting immune cells using cell centrifugation and enrichment, and Chip for detecting immune cells |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0046563B1 (en) | 1980-08-22 | 1985-04-10 | Laboratorium Prof. Dr. Rudolf Berthold | Process for performing analytical determinations by means of the chemiluminescent method, and application of this process to immuno assays |
| US4902613A (en) | 1984-05-14 | 1990-02-20 | Becton, Dickinson And Company | Lysing agent for analysis of peripheral blood cells |
| AU4406185A (en) | 1984-05-31 | 1985-12-31 | Coulter Electronics Inc. | A reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes |
| DE59708342D1 (en) | 1997-01-20 | 2002-10-31 | Orpegen Pharma Gmbh | Basophilen-degranulationstest |
| FR2778413B1 (en) | 1998-05-07 | 2000-08-04 | Immunotech Sa | NOVEL REAGENTS AND METHOD FOR LYSIS OF ERYTHROCYTES |
| CZ292686B6 (en) | 2001-09-17 | 2003-11-12 | Imumed S. R. O. | Measuring method of basophile activation after stimulation with allergen for determining allergy to the allergen and corresponding kit therefor |
| DE10235310B4 (en) | 2002-08-01 | 2012-05-16 | Molecular And Clinical Drug Research | Method for determining allergenic potency of products |
| RU2289138C2 (en) * | 2004-12-14 | 2006-12-10 | Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации | Method for allergodiagnostics by the values of chemiluminescent phosphorescence of neutrophils |
| EP2037269B1 (en) * | 2007-09-11 | 2014-04-02 | Bühlmann Laboratories AG | Allergy test based on flow cytometric analysis |
| JP2009236798A (en) | 2008-03-28 | 2009-10-15 | Sysmex Corp | Method for classifying and counting basophils |
| US20100167317A1 (en) * | 2008-08-05 | 2010-07-01 | Institut Pasteur | Process for enriching basophils in a blood sample |
| WO2012028167A2 (en) | 2010-05-20 | 2012-03-08 | Innohyphen Bv | Acridinium ester chemiluminescence upon reductive triggering |
| US20120083007A1 (en) | 2010-10-01 | 2012-04-05 | Nadeau Kari C | Basophil Activation Based Allergy Diagnostic Test |
| JP5937970B2 (en) * | 2011-01-31 | 2016-06-22 | 大鵬薬品工業株式会社 | New cypress pollen allergen |
| US20150299315A1 (en) * | 2012-10-22 | 2015-10-22 | Becton, Dickinson And Company | Non-b-lineage cells capable of producing antibody |
| ES2717509T3 (en) | 2015-01-29 | 2019-06-21 | Kjellberg Stiftung | Plasma torch |
| CN104677810B (en) * | 2015-01-30 | 2017-08-22 | 广东医学院附属医院 | The detection kit and its application method of Basohil activation |
| CN104777303B (en) * | 2015-04-29 | 2017-02-01 | 何韶衡 | Basophilic granulocyte activation and degranulation identification method |
| HUE047265T2 (en) | 2016-01-25 | 2020-04-28 | Euroimmun Medizinische Labordiagnostika Ag | A macadamia allergen |
| EP3244212A1 (en) | 2017-02-15 | 2017-11-15 | Euroimmun Medizinische Labordiagnostika AG | An improved assay for the diagnosis of peanut allergy |
-
2018
- 2018-07-26 BR BR102018015288-2A patent/BR102018015288A2/en unknown
- 2018-08-01 CA CA3012989A patent/CA3012989A1/en active Pending
- 2018-08-06 JP JP2018147570A patent/JP7265325B2/en active Active
- 2018-08-06 US US16/055,936 patent/US11119109B2/en active Active
- 2018-08-06 AU AU2018213977A patent/AU2018213977B2/en active Active
- 2018-08-06 SG SG10201806697RA patent/SG10201806697RA/en unknown
- 2018-08-06 KR KR1020180091151A patent/KR102547565B1/en active Active
- 2018-08-07 LT LTEP18187647.5T patent/LT3441765T/en unknown
- 2018-08-07 EP EP18187647.5A patent/EP3441765B1/en active Active
- 2018-08-07 DE DE102018006212.1A patent/DE102018006212A1/en not_active Withdrawn
- 2018-08-07 ES ES18187647T patent/ES2904354T3/en active Active
- 2018-08-07 PL PL18187647T patent/PL3441765T3/en unknown
- 2018-08-08 CN CN201810893515.7A patent/CN109387636B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR102018015288A2 (en) | 2021-11-09 |
| US20190049461A1 (en) | 2019-02-14 |
| JP2019032317A (en) | 2019-02-28 |
| KR20190016446A (en) | 2019-02-18 |
| CN109387636A (en) | 2019-02-26 |
| JP7265325B2 (en) | 2023-04-26 |
| KR102547565B1 (en) | 2023-06-26 |
| EP3441765B1 (en) | 2021-10-20 |
| DE102018006212A1 (en) | 2019-02-14 |
| US11119109B2 (en) | 2021-09-14 |
| AU2018213977B2 (en) | 2025-02-20 |
| CA3012989A1 (en) | 2019-02-08 |
| PL3441765T3 (en) | 2022-02-14 |
| CN109387636B (en) | 2023-07-04 |
| EP3441765A1 (en) | 2019-02-13 |
| AU2018213977A1 (en) | 2019-02-28 |
| ES2904354T3 (en) | 2022-04-04 |
| LT3441765T (en) | 2022-01-10 |
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