[go: up one dir, main page]

SG10201805928WA - Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity - Google Patents

Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity

Info

Publication number
SG10201805928WA
SG10201805928WA SG10201805928WA SG10201805928WA SG10201805928WA SG 10201805928W A SG10201805928W A SG 10201805928WA SG 10201805928W A SG10201805928W A SG 10201805928WA SG 10201805928W A SG10201805928W A SG 10201805928WA SG 10201805928W A SG10201805928W A SG 10201805928WA
Authority
SG
Singapore
Prior art keywords
metastatic
invasive
isolating
prediction
cells
Prior art date
Application number
SG10201805928WA
Inventor
Julia Kirshner
Mukti Rajen Parikh
Original Assignee
Zpredicta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zpredicta Inc filed Critical Zpredicta Inc
Publication of SG10201805928WA publication Critical patent/SG10201805928WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Physiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)

Abstract

METHODAND APPARATUS FOR ISOLATING INVASIVE AND METASTATIC CELLS FOR EVALUATING THERAPEUTICS AND PREDICTION OF METASTATIC CAPACITY Embodiments provide methods to produce an assembly that mimics in vitro the human microenvironment of a primary tumor site, a secondary distant organ, and circulation. Methods and apparatuses that provide a platform for the isolation of primary tumor, invasive, and metastatic cells to be used for evaluation of therapeutic efficacy and diagnostics as applied to metastatic cancers are also 10 provided. (NO SUITABLE FIGURE) 34
SG10201805928WA 2014-01-22 2015-01-21 Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity SG10201805928WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461930390P 2014-01-22 2014-01-22
US201562100375P 2015-01-06 2015-01-06

Publications (1)

Publication Number Publication Date
SG10201805928WA true SG10201805928WA (en) 2018-08-30

Family

ID=53681904

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201805928WA SG10201805928WA (en) 2014-01-22 2015-01-21 Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity
SG11201605858WA SG11201605858WA (en) 2014-01-22 2015-01-21 Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201605858WA SG11201605858WA (en) 2014-01-22 2015-01-21 Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity

Country Status (11)

Country Link
US (2) US10501717B2 (en)
EP (1) EP3097178A4 (en)
JP (1) JP2017506066A (en)
KR (1) KR20160106108A (en)
CN (1) CN105980541A (en)
AU (1) AU2015209434A1 (en)
BR (1) BR112016016635A2 (en)
CA (1) CA2937245A1 (en)
IL (1) IL246804A0 (en)
SG (2) SG10201805928WA (en)
WO (1) WO2015112624A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9809810B2 (en) * 2013-02-05 2017-11-07 Ohio State Innovation Foundation Galvanotaxis assay for quantitative assessment of the metastatic potential of cancer cells
US9885031B2 (en) 2013-02-05 2018-02-06 Ohio State Innovation Foundation Galvanotaxis assay for quantitative assessment of the metastatic potential of cancer cells
CA2937245A1 (en) 2014-01-22 2015-07-30 Zpredicta, Inc. Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity
KR102701228B1 (en) * 2015-10-02 2024-08-30 웨이크 포리스트 유니버시티 헬스 사이언시즈 Methods and devices for modeling cancer metastasis in vitro
US20190169562A1 (en) * 2016-07-29 2019-06-06 Zpredicta, Inc. Screening of immuno-modulatory therapies
EP4478049A3 (en) * 2016-08-03 2024-12-25 Wake Forest University Health Sciences Cancer modeling platforms and methods of using the same
CN107361024A (en) * 2017-07-18 2017-11-21 郑州大学 A kind of method for building up of esophageal squamous cell carcinoma early stage metastasis model
US12134762B2 (en) * 2017-11-09 2024-11-05 Duke University Lymphovascular invasion bioreactor and methods of making and using same
EP3746785A4 (en) 2018-02-02 2022-05-11 Wake Forest University Health Sciences, Inc. IMMUNOTHERAPY RELATED ORGANOIDS AND METHODS OF PREPARING AND USING THEREOF
AU2021284253A1 (en) * 2020-06-02 2023-01-05 Memorial Sloan Kettering Cancer Center Machine learning model for analyzing pathology data from metastatic sites
US20220276225A1 (en) * 2020-06-25 2022-09-01 Next & Bio Inc. Method for providing information necessary for diagnosing cancer patient's resistance to anti-cancer agent and/or radiation
JP2023552202A (en) * 2020-12-07 2023-12-14 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Mini organ inserts for in vitro co-culture studies
CN112986546B (en) * 2021-03-02 2022-10-18 北京大学 An impedance sensing method for monitoring colony cell invasion in three-dimensional matrices

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
WO2003027256A2 (en) 2001-09-27 2003-04-03 The Wistar Institute Methods and compositions for monitoring cell migration and identifying clinically relevant cytotoxic t lymphocyte activity
JP2006525013A (en) * 2003-05-02 2006-11-09 インセプション バイオサイエンス インコーポレイティッド Apparatus and method for amplification of the number of blood stem cells
KR20150041157A (en) * 2007-05-21 2015-04-15 웨이크 포리스트 유니버시티 헬스 사이언시즈 Progenitor cells from urine and methods for using the same
US20100273258A1 (en) * 2009-04-24 2010-10-28 The Ohio State University Interactive Microenvironment System
EP2611472B1 (en) * 2010-09-01 2016-02-10 Regents of the University of Minnesota Methods of recellularizing a tissue or organ for improved transplantability
WO2012045368A1 (en) 2010-10-08 2012-04-12 Naturin Viscofan Gmbh Cell culture insert
WO2013040445A1 (en) 2011-09-15 2013-03-21 Whitehead Institute For Biomedical Research Arrays for cell-based screening and uses thereof
WO2013116729A1 (en) 2012-02-02 2013-08-08 The Trustees Of The University Of Pennsylvania Microfabricated 3d cell culture system
CA2937245A1 (en) 2014-01-22 2015-07-30 Zpredicta, Inc. Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity
US20160178611A1 (en) 2014-04-07 2016-06-23 Bo Han Three-dimensional transglutaminase-crosslinked hydrogel for tumor engineering
US20190169562A1 (en) 2016-07-29 2019-06-06 Zpredicta, Inc. Screening of immuno-modulatory therapies

Also Published As

Publication number Publication date
IL246804A0 (en) 2016-08-31
JP2017506066A (en) 2017-03-02
EP3097178A1 (en) 2016-11-30
US10501717B2 (en) 2019-12-10
CN105980541A (en) 2016-09-28
SG11201605858WA (en) 2016-08-30
KR20160106108A (en) 2016-09-09
US11124756B2 (en) 2021-09-21
US20190284520A1 (en) 2019-09-19
BR112016016635A2 (en) 2017-08-08
CA2937245A1 (en) 2015-07-30
EP3097178A4 (en) 2017-09-06
US20160340635A1 (en) 2016-11-24
WO2015112624A1 (en) 2015-07-30
AU2015209434A1 (en) 2016-08-04

Similar Documents

Publication Publication Date Title
SG10201805928WA (en) Method and apparatus for isolating invasive and metastatic cells for evaluating therapeutics and prediction of metastatic capacity
GB2570593A (en) Methods relating to intestinal organ-on-a-chip
MX374472B (en) Tumor-infiltrating lymphocytes for adoptive cell therapy
SG11202104355SA (en) Methods for selection and stimulation of cells and apparatus for same
GB2557123A (en) Modified cells and methods of therapy
MX2018010473A (en) Combination therapy with anti-cd73 antibodies.
NZ731467A (en) Anti-tim3 antibodies and methods of use
MY197809A (en) Biomarkers and methods of treating pd-1 and pd-l1 related conditions
SG10201907501QA (en) Antibodies and assays for detection of folate receptor 1
MX2019003616A (en) SC-ß CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME.
AU2017241807A1 (en) Medium, methods, cells and secreted factors for stem cell culture and therapy
SG10201906070XA (en) Compositions and methods for high efficiency in vivo genome editing
SI3635096T1 (en) Method for the isolation of subpopulations of cardiac progenitor cells and related uses in the medical field
NZ703411A (en) Use of markers in the diagnosis and treatment of prostate cancer
MX2022008523A (en) Glycan-interacting compounds and methods of use.
HK1254735A1 (en) Device and method for obtaining immuno-stimulatory antigen-presenting cells
MX2014005800A (en) Human notch receptor mutations and their use.
TW201612308A (en) Collector architecture layout design
GB201700621D0 (en) Method,device and kit for the aseptic isolation,enrichment and stabilsation of cells from mammalian solid tissue
IL286679A (en) Ex vivo methods for producing therapeutic t cells and related compositions and methods
SG10201902594QA (en) Peptidomimetic macrocycles and uses thereof
PH12018502303A1 (en) Compositions and methods for bioengineered tissues
SG11202000851YA (en) Method for producing therapeutic exosomes from nanoelectroporation and other non-endocytic cell transfection
MX2023000191A (en) Methods of making and using pde9 inhibitors.
EP3998341A3 (en) Adenoviral vectors