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SG10201804715WA - Crispr hybrid dna/rna polynucleotides and methods of use - Google Patents

Crispr hybrid dna/rna polynucleotides and methods of use

Info

Publication number
SG10201804715WA
SG10201804715WA SG10201804715WA SG10201804715WA SG10201804715WA SG 10201804715W A SG10201804715W A SG 10201804715WA SG 10201804715W A SG10201804715W A SG 10201804715WA SG 10201804715W A SG10201804715W A SG 10201804715WA SG 10201804715W A SG10201804715W A SG 10201804715WA
Authority
SG
Singapore
Prior art keywords
methods
dna
hybrid dna
crispr
rna polynucleotides
Prior art date
Application number
SG10201804715WA
Inventor
Andrew P May
Paul D Donohoue
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of SG10201804715WA publication Critical patent/SG10201804715WA/en

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance
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    • C12N2320/53—Methods for regulating/modulating their activity reducing unwanted side-effects
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

CRISPR HYBRID DNA/RNA POLYNUCLEOTIDES AND METHODS OF USE OF THE DISCLOSURE The present disclosure provides DNA-guided CRISPR systems; polynucleotides comprising DNA, RNA and mixtures thereof for use with CRISPR systems; and methods of use involving such 5 polynucleotides and DNA-guided CRISPR systems. NO FIGURE.
SG10201804715WA 2015-01-28 2016-01-27 Crispr hybrid dna/rna polynucleotides and methods of use SG10201804715WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562108931P 2015-01-28 2015-01-28
US201562251548P 2015-11-05 2015-11-05

Publications (1)

Publication Number Publication Date
SG10201804715WA true SG10201804715WA (en) 2018-07-30

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SG11201705788TA SG11201705788TA (en) 2015-01-28 2016-01-27 Crispr hybrid dna/rna polynucleotides and methods of use
SG10201804715WA SG10201804715WA (en) 2015-01-28 2016-01-27 Crispr hybrid dna/rna polynucleotides and methods of use
SG10201906513WA SG10201906513WA (en) 2015-01-28 2016-01-27 Crispr hybrid dna/rna polynucleotides and methods of use

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SG11201705788TA SG11201705788TA (en) 2015-01-28 2016-01-27 Crispr hybrid dna/rna polynucleotides and methods of use

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SG10201906513WA SG10201906513WA (en) 2015-01-28 2016-01-27 Crispr hybrid dna/rna polynucleotides and methods of use

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US (11) US9650617B2 (en)
EP (2) EP3250691B9 (en)
JP (2) JP6835726B2 (en)
KR (4) KR20190112855A (en)
CN (2) CN111518811A (en)
AU (3) AU2016211517B2 (en)
BR (1) BR112017016080B1 (en)
CA (2) CA2975166C (en)
DK (1) DK3250691T5 (en)
ES (1) ES2955957T3 (en)
FI (1) FI3250691T3 (en)
GB (1) GB2550745A (en)
HR (1) HRP20231022T1 (en)
HU (1) HUE063813T2 (en)
IL (4) IL288263B (en)
LT (1) LT3250691T (en)
MX (2) MX2017009506A (en)
NZ (1) NZ733702A (en)
PL (1) PL3250691T3 (en)
PT (1) PT3250691T (en)
RS (1) RS64527B1 (en)
RU (1) RU2713328C2 (en)
SG (3) SG11201705788TA (en)
SI (1) SI3250691T1 (en)
SM (1) SMT202300341T1 (en)
WO (1) WO2016123230A1 (en)
ZA (2) ZA201705174B (en)

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