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SG10201803344PA - Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution - Google Patents

Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution

Info

Publication number
SG10201803344PA
SG10201803344PA SG10201803344PA SG10201803344PA SG10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA
Authority
SG
Singapore
Prior art keywords
fluorophore
chain reaction
polymerase chain
dna
quencher probes
Prior art date
Application number
SG10201803344PA
Inventor
Jackie Y Ying
Saravana Kumar Kumarasamy
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG10201803344PA publication Critical patent/SG10201803344PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Multimedia (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

NONMOTORIZED OPTICAL MULTIPLEXING FOR THE SIMULTANEOUS DETECTION OF DNA TARGET AMPLICONS IN A POLYMERASE CHAIN REACTION SOLUTION A multiplexed polymerase chain reaction (PCR) DNA detection system and a method for DNA detection within the PCR system are provided. The PCR DNA detection system includes a color charge-coupled device (CCD) camera, fluorophore- quencher probes and an imaging chamber. The fluorophore-quencher probes are selected in response to fluorophores quenched by the fluorophore-quencher probes corresponding to three selected primary colors and peak channel responses of the CCD camera such that emission profiles of the fluorophores substantially match the three selected primary colors and peak emission profiles of the fluorophores correspond to the peak RGB channel responses of the CCD camera. A DNA sample and the fluorophore-quencher probes are located within the imaging chamber. The color CCD camera is focused on the imaging chamber for simultaneous detection of up to three targets from fluorescence of the DNA sample and the fluorophore- quencher probes. FIG. . -30-
SG10201803344PA 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution SG10201803344PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SG2013076500 2013-10-14

Publications (1)

Publication Number Publication Date
SG10201803344PA true SG10201803344PA (en) 2018-06-28

Family

ID=52828473

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201803344PA SG10201803344PA (en) 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution
SG11201602873XA SG11201602873XA (en) 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201602873XA SG11201602873XA (en) 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution

Country Status (4)

Country Link
US (1) US10718012B2 (en)
CN (1) CN105960466B (en)
SG (2) SG10201803344PA (en)
WO (1) WO2015057165A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201808874XA (en) 2014-04-09 2018-11-29 Agency Science Tech & Res Microfluidic device
JP6800864B2 (en) * 2015-02-06 2020-12-16 ライフ テクノロジーズ コーポレーション Systems and methods for biological analysis
KR102843277B1 (en) * 2017-06-14 2025-08-06 유비큐디 인코포레이티드 Fiber-coupled broadband optical source
SE543211C2 (en) * 2017-06-29 2020-10-27 Mabtech Production Ab Method and system for analysing Fluorospot assays
SG10201801853WA (en) * 2018-03-02 2019-10-30 Delta Electronics Int’L Singapore Pte Ltd Portable multi-color fluorescence detection device
CN109085118A (en) * 2018-06-28 2018-12-25 陈思 A kind of novel miniaturization POCT detection device
FR3103900B1 (en) * 2019-11-29 2024-07-19 Univ Du Mans Method for rapid identification of microorganisms by analysis of excitation-emission matrices
KR20230054379A (en) * 2020-07-23 2023-04-24 라이프 테크놀로지스 코포레이션 Systems and Methods for Biological Assays

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US20040191786A1 (en) * 2001-02-16 2004-09-30 Yue David T. Three cube fret method (3-fret) for detecting fluorescence energy transfer
ATE550099T1 (en) * 2003-04-04 2012-04-15 Hoffmann La Roche IMPROVED SYSTEM FOR MULTI-COLOR REAL-TIME PCR
US7432055B2 (en) * 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
EP2021773B1 (en) * 2006-04-19 2015-03-25 IT-IS International Ltd Reaction monitoring method and apparatus
EP2225600A2 (en) * 2007-09-05 2010-09-08 Chroma Technology Corporation Light source
US8722017B2 (en) * 2008-07-10 2014-05-13 The Board Of Trustees Of The Leland Stanford Junior University Fluorescent magnetic nanoprobes, methods of making, and methods of use
DK2584344T3 (en) * 2009-01-08 2021-10-04 It Is International Ltd Optical system for detecting light from polymerase chain reactions
WO2010147654A2 (en) * 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
US8997838B2 (en) * 2009-07-10 2015-04-07 Keihin Corporation Vehicular air conditioning apparatus
US8104605B2 (en) * 2009-07-14 2012-01-31 Jansson Claes E Material sample collector
EP2633080B1 (en) * 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
CN103597093A (en) * 2010-12-29 2014-02-19 生命技术公司 Ddao compounds as fluorescent reference standards
JP2016509206A (en) * 2012-12-21 2016-03-24 マイクロニクス， インコーポレイテッド Portable fluorescence detection system and microassay cartridge

Also Published As

Publication number Publication date
US10718012B2 (en) 2020-07-21
CN105960466B (en) 2019-12-31
CN105960466A (en) 2016-09-21
US20160230213A1 (en) 2016-08-11
SG11201602873XA (en) 2016-05-30
WO2015057165A1 (en) 2015-04-23

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