[go: up one dir, main page]

SG10201801782PA - Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression - Google Patents

Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression

Info

Publication number
SG10201801782PA
SG10201801782PA SG10201801782PA SG10201801782PA SG10201801782PA SG 10201801782P A SG10201801782P A SG 10201801782PA SG 10201801782P A SG10201801782P A SG 10201801782PA SG 10201801782P A SG10201801782P A SG 10201801782PA SG 10201801782P A SG10201801782P A SG 10201801782PA
Authority
SG
Singapore
Prior art keywords
site
regulatory elements
cell assay
specific nuclease
gene expression
Prior art date
Application number
SG10201801782PA
Inventor
Stephanie Fanucchi
Youtaro Shibayama
Shaun Burd
Musa M Mhlanga
Original Assignee
Csir
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Csir filed Critical Csir
Publication of SG10201801782PA publication Critical patent/SG10201801782PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10201801782PA 2013-09-04 2014-09-04 Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression SG10201801782PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ZA201306649 2013-09-04

Publications (1)

Publication Number Publication Date
SG10201801782PA true SG10201801782PA (en) 2018-04-27

Family

ID=51626563

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201601570QA SG11201601570QA (en) 2013-09-04 2014-09-04 Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression
SG10201801782PA SG10201801782PA (en) 2013-09-04 2014-09-04 Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201601570QA SG11201601570QA (en) 2013-09-04 2014-09-04 Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression

Country Status (8)

Country Link
US (2) US10167466B2 (en)
EP (2) EP3041931B1 (en)
JP (1) JP6535333B2 (en)
KR (1) KR20160048212A (en)
AU (1) AU2014316676B2 (en)
SG (2) SG11201601570QA (en)
WO (1) WO2015033293A1 (en)
ZA (1) ZA201601434B (en)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9885033B2 (en) 2013-03-15 2018-02-06 The General Hospital Corporation Increasing specificity for RNA-guided genome editing
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
CA2930015A1 (en) 2013-11-07 2015-05-14 Editas Medicine, Inc. Crispr-related methods and compositions with governing grnas
US9840699B2 (en) 2013-12-12 2017-12-12 President And Fellows Of Harvard College Methods for nucleic acid editing
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
CN107250148B (en) 2014-12-03 2021-04-16 安捷伦科技有限公司 chemically modified guide RNA
CA2971168C (en) * 2014-12-18 2019-08-20 Csir Immunomodulation by controlling elr+ proinflammatory chemokine levels with the long non-coding rna umlilo
WO2016164356A1 (en) 2015-04-06 2016-10-13 The Board Of Trustees Of The Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
EP3337908A4 (en) * 2015-08-18 2019-01-23 The Broad Institute, Inc. METHODS AND COMPOSITIONS FOR CHANGING THE FUNCTION AND STRUCTURE OF BUCKLES AND / OR CHROMATIN DOMAINS
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and uses thereof
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
KR20250103795A (en) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Adenosine nucleobase editors and uses thereof
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
EP3500675A4 (en) 2016-08-19 2020-01-29 Whitehead Institute for Biomedical Research METHODS FOR EDITING DNA METHYLATION
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CA3035910A1 (en) 2016-09-07 2018-03-15 Flagship Pioneering, Inc. Methods and compositions for modulating gene expression
JP7588390B2 (en) 2016-10-14 2024-11-22 プレジデント アンド フェローズ オブ ハーバード カレッジ AAV Delivery of Nucleobase Editors
US20200149039A1 (en) 2016-12-12 2020-05-14 Whitehead Institute For Biomedical Research Regulation of transcription through ctcf loop anchors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
ES2949801T3 (en) 2017-01-09 2023-10-03 Whitehead Inst Biomedical Res Methods to alter gene expression by perturbation of transcription factor multimers that structure regulatory loops
CN110662556A (en) 2017-03-09 2020-01-07 哈佛大学的校长及成员们 Cancer vaccine
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
JP2020510439A (en) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ Base-editing factor from cytosine to guanine
BR112019019655A2 (en) 2017-03-23 2020-04-22 Harvard College nucleobase editors comprising nucleic acid programmable dna binding proteins
JP7379160B2 (en) 2017-04-21 2023-11-14 ザ ジェネラル ホスピタル コーポレイション Inducible and tunable multiplex human gene regulation using CRISPR-Cpf1
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
JP2020534795A (en) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ Methods and Compositions for Evolving Base Editing Factors Using Phage-Supported Continuous Evolution (PACE)
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
KR20250107288A (en) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 Uses of adenosine base editors
KR101885560B1 (en) * 2017-11-23 2018-08-06 현진이너스주식회사 High strength cover for using composite material and filler
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US11041155B2 (en) 2018-05-17 2021-06-22 The General Hospital Corporation CCCTC-binding factor variants
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US20210395831A1 (en) * 2018-11-09 2021-12-23 Arch Oncology, Inc. Biomarkers to improve efficacy of cancer immunotherapy
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
EP3942042A1 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
JP2022548399A (en) 2019-09-23 2022-11-18 オメガ セラピューティクス， インコーポレイテッド Compositions and methods for modulating hepatocyte nuclear factor 4-alpha (HNF4α) gene expression
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021226558A1 (en) 2020-05-08 2021-11-11 The Broad Institute, Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
US11884915B2 (en) 2021-09-10 2024-01-30 Agilent Technologies, Inc. Guide RNAs with chemical modification for prime editing
CN114150043A (en) * 2021-11-24 2022-03-08 吉林农业大学 Detection method of bacterial transcription inhibitor inhibiting the in vitro transcriptional activity of Escherichia coli

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003218382B2 (en) * 2002-03-21 2007-12-13 Sangamo Therapeutics, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
EP3222715A1 (en) * 2003-08-08 2017-09-27 Sangamo BioSciences, Inc. Methods and compositions for targeted cleavage and recombination
EP1526177A1 (en) * 2003-10-24 2005-04-27 Institut Curie Nucleic acids useful for triggering tumor cell lethality
HRP20161004T1 (en) * 2007-09-27 2016-10-21 Dow Agrosciences Llc Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes
WO2010014857A2 (en) * 2008-07-30 2010-02-04 University Of Massachusetts Chromosome therapy
CA2788560A1 (en) * 2010-02-08 2011-08-11 Sangamo Biosciences, Inc. Engineered cleavage half-domains
EP2925864B1 (en) * 2012-11-27 2018-10-31 The Children's Medical Center Corporation Targeting bcl11a distal regulatory elements for fetal hemoglobin reinduction

Also Published As

Publication number Publication date
AU2014316676B2 (en) 2020-07-23
JP2016529907A (en) 2016-09-29
US10167466B2 (en) 2019-01-01
KR20160048212A (en) 2016-05-03
EP3041931B1 (en) 2020-06-10
AU2014316676A1 (en) 2016-03-17
ZA201601434B (en) 2020-07-29
WO2015033293A1 (en) 2015-03-12
EP3636750A1 (en) 2020-04-15
US20190112592A1 (en) 2019-04-18
SG11201601570QA (en) 2016-04-28
EP3041931A1 (en) 2016-07-13
JP6535333B2 (en) 2019-06-26
US20160215280A1 (en) 2016-07-28

Similar Documents

Publication Publication Date Title
ZA201601434B (en) Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression
DK2922554T3 (en) Terminalt modificeret rna
SG11201406547YA (en) Nuclease-mediated targeting with large targeting vectors
IL241426B (en) Nucleic acid amplification
SG11201406717RA (en) Nucleic acid amplification
IL241269B (en) Nucleic acid amplification
GB201112140D0 (en) Nucleic acid amplification
HUE040732T2 (en) Rhizomania-resistant gene
LT2844751T (en) Improved chymosine enzyme variants
GB201213117D0 (en) Transgene expression
GB201206455D0 (en) Gene expression
IL241273A0 (en) Nucleic acid amplification
EP2814944A4 (en) Biomass yield genes
IL245687A0 (en) Nucleic acid amplification
IL236116A0 (en) Controlled gene expression methods
ZA201405621B (en) Neck-down feeder
EP2831915A4 (en) Dna computing
PL2978847T3 (en) Hammerhead ribozymes targeting mir-21
GB201309132D0 (en) Regulatable gene expression
GB201315714D0 (en) DNA Construct
GB201307838D0 (en) Improved nucleic acid expressions
GB201306288D0 (en) Gene targeting in animals
GB201201397D0 (en) Gene targeting in animals
IL240312A0 (en) Positively charged oplysaccharides for rna transfection
HU4193U (en) Laboratorial photo-bioreactor