SE8900179D0 - ENZYMATIC PEPTIDE SYNTHESIS WITH MURAMOYL PENTAPETIDE CARBOXIPEPTIDAS - Google Patents
ENZYMATIC PEPTIDE SYNTHESIS WITH MURAMOYL PENTAPETIDE CARBOXIPEPTIDASInfo
- Publication number
- SE8900179D0 SE8900179D0 SE8900179A SE8900179A SE8900179D0 SE 8900179 D0 SE8900179 D0 SE 8900179D0 SE 8900179 A SE8900179 A SE 8900179A SE 8900179 A SE8900179 A SE 8900179A SE 8900179 D0 SE8900179 D0 SE 8900179D0
- Authority
- SE
- Sweden
- Prior art keywords
- carboxipeptidas
- pentapetide
- muramoyl
- peptide synthesis
- derivative
- Prior art date
Links
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 238000010647 peptide synthesis reaction Methods 0.000 title 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 abstract 2
- 125000000217 alkyl group Chemical group 0.000 abstract 2
- 150000001412 amines Chemical class 0.000 abstract 2
- 239000003960 organic solvent Substances 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 102000005367 Carboxypeptidases Human genes 0.000 abstract 1
- 108010006303 Carboxypeptidases Proteins 0.000 abstract 1
- GSNUFIFRDBKVIE-UHFFFAOYSA-N DMF Natural products CC1=CC=C(C)O1 GSNUFIFRDBKVIE-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 239000012062 aqueous buffer Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 125000006239 protecting group Chemical group 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The use of muramoylpentapeptide carboxypeptides for enzymatic synthesis of peptides and peptide derivatives is new. Coupling takes place in water or organic solvent, at (apparent) pH between 4 and 11 and in temp. range 0-70 deg.C, according to the scheme: R1-A-R2 + H-B-R3 -- = R1-A-B-R3 + H-R2. - Where R1 is H or a protecting group e.g. acetyl, A is an amino acid or derivative with D-configuration, R2 is OX where X is alkyl or other gp., B is an amino acid or derivative with D-configuration and R3 is OH, NK2, or OX (where X is e.g. Alkyl). - Further use of aqueous buffer or blends with organic solvents inc. methanol, ethanol, DMF, DMSO are particularly claimed. The use of mumoylpentapeptide carboxypeptidase isolated from bacteria is also claimed. The enzyme may be used in free or immobilised form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8900179A SE8900179L (en) | 1989-01-19 | 1989-01-19 | Enzymatic peptide synthesis - using muramoyl penta:peptide carboxy:peptidase to improve yield and stereospecificity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8900179A SE8900179L (en) | 1989-01-19 | 1989-01-19 | Enzymatic peptide synthesis - using muramoyl penta:peptide carboxy:peptidase to improve yield and stereospecificity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8900179D0 true SE8900179D0 (en) | 1989-01-19 |
| SE8900179L SE8900179L (en) | 1990-07-20 |
Family
ID=20374789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8900179A SE8900179L (en) | 1989-01-19 | 1989-01-19 | Enzymatic peptide synthesis - using muramoyl penta:peptide carboxy:peptidase to improve yield and stereospecificity |
Country Status (1)
| Country | Link |
|---|---|
| SE (1) | SE8900179L (en) |
-
1989
- 1989-01-19 SE SE8900179A patent/SE8900179L/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| SE8900179L (en) | 1990-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Barlos et al. | Efficient" one-pot" synthesis of N-tritylamino acids | |
| Hofmann et al. | Studies on polypeptides. XXXIV. Enzymic properties of partially synthetic De (16-20)-and De (15-20)-ribonucleases S'1-3 | |
| MX9304970A (en) | Ss-AMINOALKYL- AND ß-N-PEPTIDYLAMINOALKYL-BORONIC ACIDS. | |
| IE60582B1 (en) | Novel peptidase inhibitors | |
| ATE81854T1 (en) | AMINOLUCIFERINS, PROCESS FOR THEIR PRODUCTION AND THEIR USE. | |
| DE69932424D1 (en) | MELANOCORTIN 1 RECEPTOR-SELECTIVE COMPOUNDS | |
| HRP980124A2 (en) | Dolastatin 15 derivatives with carbonyl and heterocyclic functionalities at the c-terminus | |
| SHIOIRI et al. | Amino acids and peptides. XI. Phosphorus in organic synthesis. VI. Application of diphenyl phosphorazidate to the synthesis of peptides containing various functions | |
| DE69830931D1 (en) | Process for the preparation of 1,4,7,10-tetraazadodecane-1,4-diacetic acid derivatives | |
| SE8900179D0 (en) | ENZYMATIC PEPTIDE SYNTHESIS WITH MURAMOYL PENTAPETIDE CARBOXIPEPTIDAS | |
| GB927714A (en) | New cyclic octapeptides | |
| CA2274852A1 (en) | Method of coupling polysaccharides to proteins | |
| CA1247088A (en) | Oligopeptide prodrugs | |
| DE69830307D1 (en) | PROCESS FOR PREPARING PHARMACOLOGICALLY COMPATIBLE SALTS OF N- (1 (S) -ETHOXYCARBONYL-3-PHENYLPROPYL) -L-ALANYL AMINO ACIDS | |
| Sugano et al. | Studies of peptide antibiotics. XXX. Syntheses of gramicidin S analogs containing N-methylleucine in place of leucine | |
| Uramoto et al. | A new antifungal antibiotic, cystargin: fermentation, isolation, and characterization | |
| ATE448249T1 (en) | NEW CYCLODEXTRINE DIMERES AND DERIVATIVES THEREOF, PRODUCTION PROCESS THEREOF AND USE THEREOF, IN PARTICULAR FOR THE SOLUBILIZATION OF PHARMACOLOGICAL ACTIVE INGREDIENTS | |
| Abe et al. | Studies of peptide antibiotics. XXXIII. Syntheses of gramicidin S analogs containing N-methyl-L-valine in place of L-valine. | |
| HU197757B (en) | Process for producing chromogen compounds | |
| KR930021643A (en) | Improved Manufacturing Method of Cephalosporins | |
| NO994929D0 (en) | Synthetic peptides, useful in biological tests, for detecting infections caused by group 0 HIV-1 virus | |
| NO963255L (en) | Cyclic neurokinin-A antagonists | |
| WO1997047313A1 (en) | Total synthesis of bacitracin polypetides | |
| DE58908128D1 (en) | Connections for the introduction of an SH group in tyrosine. | |
| US4351828A (en) | N-Substituted cyclopeptide derivatives |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAV | Patent application has lapsed |
Ref document number: 8900179-6 Effective date: 19930801 |