SE502995C2 - Spectrophotometer equipment and its use - Google Patents
Spectrophotometer equipment and its useInfo
- Publication number
- SE502995C2 SE502995C2 SE9402354A SE9402354A SE502995C2 SE 502995 C2 SE502995 C2 SE 502995C2 SE 9402354 A SE9402354 A SE 9402354A SE 9402354 A SE9402354 A SE 9402354A SE 502995 C2 SE502995 C2 SE 502995C2
- Authority
- SE
- Sweden
- Prior art keywords
- cuvette
- light
- wall
- equipment according
- detector
- Prior art date
Links
- 239000000523 sample Substances 0.000 claims abstract description 17
- 230000003287 optical effect Effects 0.000 claims abstract description 10
- 230000031700 light absorption Effects 0.000 claims abstract description 4
- 238000005259 measurement Methods 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 5
- 239000008187 granular material Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000006072 paste Substances 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 239000010453 quartz Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
(TI J Po 1") VÄ U l orsakar stora variationer i absorbansnivåerna såväl mellan olika prover som mellan olika mätvärden för ett och samma prov. Provernas varierande struktur medför liknande problem på grund av varierande packningstäthet. (TI J Po 1 ") VÄ U l causes large variations in the absorbance levels both between different samples and between different measured values for one and the same sample. The varying structure of the samples causes similar problems due to varying packing density.
Proverna har därför hittills inte kunnat analyseras på ett tillfredsställande sätt med sådana instrument.The samples have therefore so far not been able to be analyzed satisfactorily with such instruments.
Genom föreliggande uppfinning har man nu helt överraskande kunnat lösa ovanstående problem och åstadkommit en spektro- fotometerutrustning innefattande en ljuskälla placerad framför en kyvett samt en detektor, placerad bakom kyvetten, vilken detektor mäter ljusabsorption från ljuskällan i ett provmaterial anbringat i kyvetten varvid en del av ljuset passerar genom en främre transparent vägg på kyvetten, provmaterialet samt en bakre transparent vägg på kuvetten innan ljuset mäts i detektorn. Utrustningen kännetecknas av att den främre och/eller den bakre väggen på kyvetten bildar en sned vinkel i förhållande till den optiska axeln för det utsända ljuset. Kyvetten får därmed ett kilformat utseende. Åtminstone kyvettens främre och bakre transparenta väggar är lämpligen tillverkade av glas, kvarts eller annat material som är transparent för det för mätning utnyttjade våglängdsområdet.The present invention has now quite surprisingly solved the above problems and provided a spectrophotometer equipment comprising a light source located in front of a cuvette and a detector located behind the cuvette, which detector measures light absorption from the light source in a sample material applied to the cuvette. passes through a front transparent wall on the cuvette, the sample material and a rear transparent wall on the cuvette before the light is measured in the detector. The equipment is characterized in that the front and / or the rear wall of the cuvette forms an oblique angle relative to the optical axis of the emitted light. The cuvette thus has a wedge-shaped appearance. At least the front and rear transparent walls of the cuvette are suitably made of glass, quartz or other material which is transparent to the wavelength range used for measurement.
Enligt en föredragen utföringsform av uppfinningen är ky- vetten utformad så att dess främre vägg bildar en sned vinkel i förhållande till den optiska axeln för det från ljuskällan utsända ljuset medan den bakre väggen bildar en rät eller huvudsakligen rät vinkel i förhållande till nämnda optiska axel. xïl (_ I\J 995 Ovanstående utformning av kyvetten enligt uppfinningen har givit den stora fördelen att man uppnått en mindre variation mellan absorbansnivåerna för olika prover och provtyper samt att skillnaden mellan det högsta och lägsta värdet för varje spektra har minskat. Därmed kan enligt uppfinningen en enda transmissionsspektrofotometer med nämnda kyvett klara av att analysera många olika typer av material på ett mycket tillförlitligt sätt.According to a preferred embodiment of the invention, the cuvette is designed so that its front wall forms an oblique angle relative to the optical axis of the light emitted from the light source while the rear wall forms a right or substantially right angle relative to said optical axis. xïl (_ I \ J 995 The above design of the cuvette according to the invention has given the great advantage that a smaller variation between the absorbance levels for different samples and sample types has been achieved and that the difference between the highest and lowest value for each spectra has decreased. a single transmission spectrophotometer with said cuvette capable of analyzing many different types of materials in a very reliable manner.
Den använda ljuskällan arbetar oftast inom våglängds- området 800-1100 nm, vilket är nära det infraröda ljuset.The light source used usually operates within the wavelength range 800-1100 nm, which is close to the infrared light.
Ljuset som passerar genom provet i kuvetten har en relativt liten utbredning vid ingången i provet men sprids fort genom reflektion och riktningsändring så snart det kommit en bit in i provet. Normalt går mellan l/100 och 1/100000 av det ingående ljuset genom provet om detta föreligger i finpulvriserad form, vilket är en föredragen utföringsform enligt uppfinningen. För att få reproducerbara analysvärden är det därvid ofta nödvändigt att finmala materialet och sikta det så att samma eller huvudsakligen samma kornstorleksfördelning erhålles på varje prov.The light that passes through the sample in the cuvette has a relatively small propagation at the entrance to the sample but is spread quickly by reflection and change of direction as soon as it has entered the sample. Normally between 1/100 and 1/100000 of the input light passes through the sample if it is in finely powdered form, which is a preferred embodiment according to the invention. In order to obtain reproducible analysis values, it is often necessary to grind the material and aim it so that the same or substantially the same grain size distribution is obtained on each sample.
Genom att kyvetten enligt ovan är kilformad möjliggörs också en förenklad ifyllning och packning av det pulverformade materialet i kyvetten.Because the cuvette according to the above is wedge-shaped, a simplified filling and packing of the powder-shaped material in the cuvette is also possible.
Provmaterialet kan även föreligga som ett granulat, en slurry, en pasta eller en vätska.The sample material may also be present as a granulate, a slurry, a paste or a liquid.
Uppfinningen innefattar även en användning av ovanstående spektrofotometerutrustning för analys av bl.a. fodervaror UI C) ru \O \O Un såsom nötfoder etc. samt födoämnen varvid dessa produkter från början föreligger som granulat, finmalt pulver, slurry, pasta eller vätska eller överförs till någon av dessa former före analysen.The invention also comprises a use of the above spectrophotometer equipment for analysis of e.g. feed materials UI C) ru \ O \ O Un such as beef feed, etc. and foods in which these products are initially present as granules, finely ground powder, slurry, paste or liquid or are transferred to any of these forms before analysis.
Utrustningen enligt uppfinningen är mycket lämplig för analys av prover innehållande fett och/eller protein.The equipment according to the invention is very suitable for analysis of samples containing fat and / or protein.
Därvid används företrädesvis ett finmalt material som ovan nämnts.In this case, a finely ground material as mentioned above is preferably used.
Uppfinningen förklaras närmare i anslutning till bifogade figur som schematiskt från sidan visar en utföringsform av en spektrofotometerutrustning enligt uppfinningen innefattande en ljuskälla l av monokromatisk typ placerad framför en kyvett 2 samt en detektor 3 placerad bakom ky- vetten 2. I kyvetten 2 är ett finmalt pulverformigt foder- material 4 anbringat. Ljuset från ljuskällan 1 kommer att passera en främre transparent vägg 5 på kyvetten, foder- materialet 4 samt en bakre transparent vägg 6 på kyvetten innan ljuset mäts i detektorn 3. Den främre väggen 5 på kyvetten 2 bildar en sned vinkel i förhållande till den optiska axeln 7 för det utsända ljuset.The invention is explained in more detail in connection with the attached figure which schematically shows from the side an embodiment of a spectrophotometer equipment according to the invention comprising a light source 1 of monochromatic type placed in front of a cuvette 2 and a detector 3 placed behind the cuvette 2. In the cuvette 2 a finely ground powder is feed material 4 applied. The light from the light source 1 will pass a front transparent wall 5 on the cuvette, the lining material 4 and a rear transparent wall 6 on the cuvette before the light is measured in the detector 3. The front wall 5 on the cuvette 2 forms an oblique angle relative to the optical axis 7 of the emitted light.
Den uppmätta ljusabsorptionen räknas sedan om till önskade parametrar med hjälp av olika statistiska metoder.The measured light absorption is then converted to the desired parameters using various statistical methods.
Analysvärdena blir betydligt tillförlitligare än vid jäm- förande försök med en konventionell kyvett där såväl den främre som den bakre väggen bildar en rät vinkel i för- hållande till den optiska axeln för det utsända ljuset från ljuskällan.The analysis values become significantly more reliable than in comparative experiments with a conventional cuvette where both the front and the rear wall form a right angle in relation to the optical axis of the emitted light from the light source.
(Tï (I) NJ af.) \,_O ( N Uppfinningen är inte begränsad till de visade utförings- formerna, då dessa kan modifieras på olika sätt inom upp- finningens ram. Kyvettens främre och/eller bakre vägg som normalt är plana kan således i stället utformas som en del av en cylinder eller sfär. Väggen/väggarna får därvid automatiskt en sned vinkel i förhållande till den optiska axeln för det utsända ljuset. Användningen är naturligtvis inte begränsad till analys av fodervaror och födoämnen.(Tï (I) NJ af.) \, _ O (N The invention is not limited to the embodiments shown, as these can be modified in various ways within the scope of the invention. The front and / or rear wall of the cuvette which is normally flat can thus the wall wall (s) are automatically formed at an oblique angle with respect to the optical axis of the emitted light.The use is of course not limited to the analysis of feedstuffs and foodstuffs.
Spektrofotometerutrustningen enligt uppfinningen är lika användbar för samtliga provtyper där man har ovan beskrivna mättekniska problem.The spectrophotometer equipment according to the invention is equally useful for all sample types where the measurement technical problems described above are present.
Claims (4)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9402354A SE502995C2 (en) | 1994-07-04 | 1994-07-04 | Spectrophotometer equipment and its use |
| AU29413/95A AU2941395A (en) | 1994-07-04 | 1995-06-30 | Spectrophotometric equipment and use thereof |
| PCT/SE1995/000808 WO1996001417A1 (en) | 1994-07-04 | 1995-06-30 | Spectrophotometric equipment and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9402354A SE502995C2 (en) | 1994-07-04 | 1994-07-04 | Spectrophotometer equipment and its use |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE9402354D0 SE9402354D0 (en) | 1994-07-04 |
| SE9402354L SE9402354L (en) | 1996-01-05 |
| SE502995C2 true SE502995C2 (en) | 1996-03-04 |
Family
ID=20394618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE9402354A SE502995C2 (en) | 1994-07-04 | 1994-07-04 | Spectrophotometer equipment and its use |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU2941395A (en) |
| SE (1) | SE502995C2 (en) |
| WO (1) | WO1996001417A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999023473A1 (en) * | 1997-10-31 | 1999-05-14 | Foss Electric A/S | A cuvette and spacer therefor as well as a method of producing the spacer |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5893288A (en) * | 1997-04-25 | 1999-04-13 | Morgan Construction Company | Multiple outlet finishing mill |
| SE9802855L (en) * | 1998-08-26 | 2000-02-27 | Alfa Laval Agri Ab | Fluid sensing device and method especially for use in milking machines |
| CN110044829A (en) * | 2019-05-08 | 2019-07-23 | 上海仪电物理光学仪器有限公司 | Food quality analyzer and its application method |
| EP4049756A1 (en) * | 2021-02-24 | 2022-08-31 | Paul Marienfeld GmbH & Co. KG | Container for a sample and method for analyzing a sample with the container |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2521236C3 (en) * | 1975-05-10 | 1978-12-14 | Hildegard Dr. 4400 Muenster Goehde Geb. Kuhl | Device for counting and measuring particles suspended in a liquid |
| EP0056821A1 (en) * | 1980-07-24 | 1982-08-04 | Labsystems Oy | Method of measurement and a cuvette |
| US5427959A (en) * | 1990-10-01 | 1995-06-27 | Canon Kabushiki Kaisha | Apparatus and method for measuring specimen |
| GB9201348D0 (en) * | 1992-01-22 | 1992-03-11 | Rooney Matthew N | Assessment of characteristics of fluids |
-
1994
- 1994-07-04 SE SE9402354A patent/SE502995C2/en not_active IP Right Cessation
-
1995
- 1995-06-30 WO PCT/SE1995/000808 patent/WO1996001417A1/en not_active Ceased
- 1995-06-30 AU AU29413/95A patent/AU2941395A/en not_active Abandoned
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999023473A1 (en) * | 1997-10-31 | 1999-05-14 | Foss Electric A/S | A cuvette and spacer therefor as well as a method of producing the spacer |
| US6573988B1 (en) | 1997-10-31 | 2003-06-03 | Foss Electric A/S | Cuvette and spacer therefor as well as a method of producing the spacer |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1996001417A1 (en) | 1996-01-18 |
| AU2941395A (en) | 1996-01-25 |
| SE9402354D0 (en) | 1994-07-04 |
| SE9402354L (en) | 1996-01-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NUG | Patent has lapsed |