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SE2450041A1 - Capture surface of metapolonies - Google Patents

Capture surface of metapolonies

Info

Publication number
SE2450041A1
SE2450041A1 SE2450041A SE2450041A SE2450041A1 SE 2450041 A1 SE2450041 A1 SE 2450041A1 SE 2450041 A SE2450041 A SE 2450041A SE 2450041 A SE2450041 A SE 2450041A SE 2450041 A1 SE2450041 A1 SE 2450041A1
Authority
SE
Sweden
Prior art keywords
capture surface
target molecule
sample
metapolonies
creating
Prior art date
Application number
SE2450041A
Other versions
SE547614C2 (en
Inventor
Erik Benson
Ian Torao Hoffecker
Original Assignee
Erik Benson
Ian Torao Hoffecker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Erik Benson, Ian Torao Hoffecker filed Critical Erik Benson
Priority to SE2450041A priority Critical patent/SE547614C2/en
Priority to PCT/EP2025/050806 priority patent/WO2025153496A1/en
Publication of SE2450041A1 publication Critical patent/SE2450041A1/en
Publication of SE547614C2 publication Critical patent/SE547614C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for creating a capture surface for at least one target molecule, such as an mRNA molecule, in a sample, wherein each target molecule comprises a target nucleic acid region. The invention also relates to a capture surface for said at least one target molecule that is suitable for determining spatial location of the at least one target molecule in the sample, a kit comprising said capture surface as well as a method for creating a spatial map of said at least one target molecule present in the sample using said capture surface.
SE2450041A 2024-01-15 2024-01-15 Capture surface of metapolonies SE547614C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE2450041A SE547614C2 (en) 2024-01-15 2024-01-15 Capture surface of metapolonies
PCT/EP2025/050806 WO2025153496A1 (en) 2024-01-15 2025-01-14 Mesh chip

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE2450041A SE547614C2 (en) 2024-01-15 2024-01-15 Capture surface of metapolonies

Publications (2)

Publication Number Publication Date
SE2450041A1 true SE2450041A1 (en) 2025-07-16
SE547614C2 SE547614C2 (en) 2025-10-28

Family

ID=94382788

Family Applications (1)

Application Number Title Priority Date Filing Date
SE2450041A SE547614C2 (en) 2024-01-15 2024-01-15 Capture surface of metapolonies

Country Status (2)

Country Link
SE (1) SE547614C2 (en)
WO (1) WO2025153496A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190203275A1 (en) * 2015-04-10 2019-07-04 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US20200277663A1 (en) * 2018-12-10 2020-09-03 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
EP3541956B1 (en) * 2016-11-17 2021-07-07 Spatial Transcriptomics AB Method for spatial tagging and analysing nucleic acids in a biological specimen
US20210317524A1 (en) * 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE545710T1 (en) 1997-04-01 2012-03-15 Illumina Cambridge Ltd METHOD FOR THE DUPLICATION OF NUCLEIC ACIDS
CN116097360A (en) * 2020-07-17 2023-05-09 密执安大学评议会 Materials and methods for localized detection of nucleic acids in tissue samples
US20240158854A1 (en) * 2021-03-12 2024-05-16 University Of Washington High-resolution spatial transcriptome

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190203275A1 (en) * 2015-04-10 2019-07-04 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP3541956B1 (en) * 2016-11-17 2021-07-07 Spatial Transcriptomics AB Method for spatial tagging and analysing nucleic acids in a biological specimen
US20210317524A1 (en) * 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US20200277663A1 (en) * 2018-12-10 2020-09-03 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Cheng M, et al. "Spatially resolved transcriptomics: a comprehensive review of their technological advances, applications, and challenges". J Genet Genomics. 2023;50(9):625-640 *
Cho CS, et al. "Microscopic examination of spatial transcriptome using Seq-Scope". Cell. 2021;184(13):3559-3572.e22 *
Hoffecker IT, et al. "A computational framework for DNA sequencing microscopy". Proc Natl Acad Sci U S A. 2019;116(39):19282-19287 *

Also Published As

Publication number Publication date
SE547614C2 (en) 2025-10-28
WO2025153496A1 (en) 2025-07-24

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