SE0401626L - A method for establishing a pluripotent human stem cell line derived from blastocytes - Google Patents
A method for establishing a pluripotent human stem cell line derived from blastocytesInfo
- Publication number
- SE0401626L SE0401626L SE0401626A SE0401626A SE0401626L SE 0401626 L SE0401626 L SE 0401626L SE 0401626 A SE0401626 A SE 0401626A SE 0401626 A SE0401626 A SE 0401626A SE 0401626 L SE0401626 L SE 0401626L
- Authority
- SE
- Sweden
- Prior art keywords
- cell line
- blastocyst
- stem cell
- cells
- pluripotent human
- Prior art date
Links
- 210000000130 stem cell Anatomy 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 2
- 210000001109 blastomere Anatomy 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 8
- 210000002459 blastocyst Anatomy 0.000 abstract 7
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 4
- 238000002360 preparation method Methods 0.000 abstract 3
- 102000004877 Insulin Human genes 0.000 abstract 2
- 108090001061 Insulin Proteins 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 229940125396 insulin Drugs 0.000 abstract 2
- 230000003178 anti-diabetic effect Effects 0.000 abstract 1
- 230000000648 anti-parkinson Effects 0.000 abstract 1
- 239000003472 antidiabetic agent Substances 0.000 abstract 1
- 239000000939 antiparkinson agent Substances 0.000 abstract 1
- 239000000824 cytostatic agent Substances 0.000 abstract 1
- 230000001085 cytostatic effect Effects 0.000 abstract 1
- 238000002224 dissection Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 230000010534 mechanism of action Effects 0.000 abstract 1
- 230000000324 neuroprotective effect Effects 0.000 abstract 1
- 230000001777 nootropic effect Effects 0.000 abstract 1
- 210000000287 oocyte Anatomy 0.000 abstract 1
- 230000001902 propagating effect Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/166—Animal cells resulting from interspecies fusion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Reproductive Health (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Gynecology & Obstetrics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Novel method for obtaining a pluripotent human blastocyst-derived stem cell line. - Obtaining a pluripotent human blastocyst-derived stem cell line comprises steps (i-v): - (i) using a fertilized oocyte optionally, having a grade 1 or 2, to obtain a blastocyst, optionally having a grade A or B; - (ii) co-culturing the blastocyst with feeder cells for establishing one or more colonies of inner cell mass cells; - (iii) isolating the inner cell mass cells by mechanical dissection; - (iv) co-culturing the inner cell mass cells with feeder cells to obtain a blastocyst-derived stem cell line; and optionally - (v) propagating the blastocyst-derived stem cell line. - Independent claims are also included for the following: - (1) a preparation of differentiated stem cells; - (2) producing a pure preparation of insulin-producing differentiated stem cells; and - (3) a kit for producing a preparation of pluripotent human blastocyst-derived stem cell line or insulin-producing differentiated stem cells. - ACTIVITY - Cytostatic; Neuroprotective; Antidiabetic; Antiparkinsonian; Nootropic. No biological data given. - MECHANISM OF ACTION - Gene therapy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0401626A SE0401626L (en) | 2001-12-28 | 2004-06-23 | A method for establishing a pluripotent human stem cell line derived from blastocytes |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34277101P | 2001-12-28 | 2001-12-28 | |
| SE0104471A SE0104471D0 (en) | 2001-12-28 | 2001-12-28 | New method |
| PCT/EP2002/014895 WO2003055992A2 (en) | 2001-12-28 | 2002-12-27 | A method for the establishment of a pluripotent human blastocyst-derived stem cell line |
| SE0401626A SE0401626L (en) | 2001-12-28 | 2004-06-23 | A method for establishing a pluripotent human stem cell line derived from blastocytes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE0401626D0 SE0401626D0 (en) | 2004-06-23 |
| SE0401626L true SE0401626L (en) | 2004-08-25 |
Family
ID=32912859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE0401626A SE0401626L (en) | 2001-12-28 | 2004-06-23 | A method for establishing a pluripotent human stem cell line derived from blastocytes |
Country Status (1)
| Country | Link |
|---|---|
| SE (1) | SE0401626L (en) |
-
2004
- 2004-06-23 SE SE0401626A patent/SE0401626L/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| SE0401626D0 (en) | 2004-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| GB9308271D0 (en) | Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method | |
| WO2002078449A3 (en) | Method for facilitating the production of differentiated cell types and tissues from embryonic and adult pluripotent and multipotent cells | |
| SG152273A1 (en) | Pdx1 expressing endoderm | |
| WO2003016507A3 (en) | Generation of multipotent central nervous system stem cells | |
| EP1534066A4 (en) | Alpha(1,3)-galactosyltransferase null cells, methods of selecting and alpha(1,3)-galactosyltransferase null swine produced therefrom | |
| SG149061A1 (en) | Definitive endoderm | |
| NZ526236A (en) | Isoindolin-1-one glucokinase activators and their use in pharmaceutical compositions for the treatment of type II diabetes | |
| SG129236A1 (en) | Somatic pluripotent cells | |
| CY1108477T1 (en) | METHODS FOR PRODUCING INSULIN IN PLANTS | |
| DK0780469T3 (en) | Immortalized cell lines derived from normal human skin tissue and serum-free growth medium for growing such | |
| NL1001620C2 (en) | Improvement in activity of plant growth regulators | |
| WO2007042225A3 (en) | A method for obtaining a xeno-free hbs cell line | |
| WO2006108882A8 (en) | In vitro production of a cell population using feeder cells | |
| WO2002086082A3 (en) | Isolation of neural stem cells using gangliosides and other surface markers | |
| SG137871A1 (en) | In vitro techniques for use with stem cells | |
| CY1111775T1 (en) | COTTON LUNG CELLULAR CELLS FOR VIRUSUAL CULTIVATION | |
| EP2275530A3 (en) | Method for cultivating cells for the production of compounds | |
| WO2004011596A3 (en) | Method for preparing an extracellular matrix and its use for tumor cell culture | |
| AU8611401A (en) | Improvements in or relating to selective agents for biological cultures | |
| SE0401626L (en) | A method for establishing a pluripotent human stem cell line derived from blastocytes | |
| EP1270719A3 (en) | Cell population containing non-fetal hemangioblasts and method for producing same | |
| AU2001274540A1 (en) | Monkey-origin embryonic stem cells | |
| WO2005054447A3 (en) | Müller stem cells | |
| IL137410A0 (en) | A method for the production of transgenic plants using apical shoot tips | |
| WO2002036748A3 (en) | Methods for depleting and isolating alloreactive and antigen-reactive t cells from hematopoietic donor cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAV | Patent application has lapsed | ||
| NAV | Patent application has lapsed |