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SE0200695D0 - New method - Google Patents

New method

Info

Publication number
SE0200695D0
SE0200695D0 SE0200695A SE0200695A SE0200695D0 SE 0200695 D0 SE0200695 D0 SE 0200695D0 SE 0200695 A SE0200695 A SE 0200695A SE 0200695 A SE0200695 A SE 0200695A SE 0200695 D0 SE0200695 D0 SE 0200695D0
Authority
SE
Sweden
Prior art keywords
nucleic acid
cystic fibrosis
typing
test pattern
acid molecule
Prior art date
Application number
SE0200695A
Other languages
Swedish (sv)
Inventor
Anna-Lotta Schiller
Jenny Dunker
Original Assignee
Pyrosequencing Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pyrosequencing Ab filed Critical Pyrosequencing Ab
Priority to SE0200695A priority Critical patent/SE0200695D0/en
Publication of SE0200695D0 publication Critical patent/SE0200695D0/en
Priority to AU2003210099A priority patent/AU2003210099A1/en
Priority to PCT/SE2003/000394 priority patent/WO2003074737A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for typing of at least one nucleic acid molecule of the human cystic fibrosis transmembrane conductance regulator CFTR gene comprising the steps of: providing at least one nucleic acid molecule of the CFTR gene providing at least one extension primer designed to extend over at least two potential variable sites in the nucleic acid molecules simultaneously or sequentially performing primer extension reactions, and determining the pattern of nucleotide incorporation to obtain a test pattern optionally comparing the test pattern of step c with one or more reference patterns, in order to type the variable sites of the nucleic acid molecules. Moreover, the invention refers to a kit comprising a set of primers for use in typing mutations related to cystic fibrosis. The invention hereby provides an easy, reliable and fast system for analysis of the large number of mutations related to cystic fibrosis.
SE0200695A 2002-03-07 2002-03-07 New method SE0200695D0 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE0200695A SE0200695D0 (en) 2002-03-07 2002-03-07 New method
AU2003210099A AU2003210099A1 (en) 2002-03-07 2003-03-07 New method
PCT/SE2003/000394 WO2003074737A1 (en) 2002-03-07 2003-03-07 New method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0200695A SE0200695D0 (en) 2002-03-07 2002-03-07 New method

Publications (1)

Publication Number Publication Date
SE0200695D0 true SE0200695D0 (en) 2002-03-07

Family

ID=20287196

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0200695A SE0200695D0 (en) 2002-03-07 2002-03-07 New method

Country Status (3)

Country Link
AU (1) AU2003210099A1 (en)
SE (1) SE0200695D0 (en)
WO (1) WO2003074737A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10356783A1 (en) * 2003-12-02 2005-07-07 Universität Zu Köln Method for multiplex sequencing
AU2013319788B2 (en) 2012-09-24 2019-04-11 Yissum Reasearch Development Company of the Hebrew University Of Jerusalem Ltd. Restoration of the CFTR function by splicing modulation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018177A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia Diagnosis of cystic fibrosis using allele specific multiplex polymerase chain reactions
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9800536D0 (en) * 1998-01-13 1998-03-11 Zeneca Ltd Sequences

Also Published As

Publication number Publication date
AU2003210099A1 (en) 2003-09-16
WO2003074737A1 (en) 2003-09-12

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