RU99127287A - METHODS AND COMPOSITIONS FOR IDENTIFICATION OF PIGS GENETICALLY RESISTANT TO DISEASES ASSOCIATED WITH F18E. Coli - Google Patents

Claims (18)

1. A method for identifying pigs that are resistant to intestinal colonization of E. coli, wherein said method comprises detecting the presence of genetic polymorphism associated with resistance to bacterial colonization in a biological sample taken from pigs, and establishing that the pig is resistant in the event that this pig is homozygous for this polymorphism.  2. A method for identifying pigs resistant to intestinal diseases associated with E. coli, wherein the method comprises determining whether the nitrogen base at position 307 of the alpha (1,2) -fucosyl transferase 1 gene is in a biological sample from a pig , only adenine, and identification of pigs as resistant, if at position 307 only adenine is the nitrogenous base.  3. A method for breeding pigs resistant to diseases associated with E. coli, wherein said method comprises selecting for breeding pigs that exhibit genetic polymorphism for the alpha (1,2) -fucosyl transferase 1 gene, which makes it possible to identify them as pigs, resistant to intestinal diseases associated with E. coli, and breeding of selected pigs.  4. The method according to p. 1, 2 or 3, where E. coli is strain F18.  5. An isolated DNA molecule that is polymorphic for the porcine gene of alpha- (1,2) -fucosyltransferase 1.  6. An isolated DNA molecule having the nucleotide sequence shown in FIG. 1.  7. The isolated DNA molecule according to claim 6, wherein at position 307 adenine is present instead of guanine.  8. The selected DNA molecule, which is a complementary nucleotide sequence according to claim 6.  9. The isolated DNA molecule according to claim 6, where in the nucleotide position of 887 guanine is replaced by adenine.  10. The isolated DNA molecule according to claim 6, wherein threonine is present at nucleotide position 229.  11. The polypeptide encoded by the DNA molecule according to claim 5 or 6, where the specified molecule has alpha- (1,2) -fucosyltransferase activity.  12. A portion of the isolated DNA molecule according to claim 5 or 6, wherein said portion includes a nucleotide sequence that allows identification of E. coli resistant pigs from E. coli susceptible pigs.  13. Molecular analysis to detect E. coli F18 receptors in pigs, wherein said analysis provides for the isolation of DNA from porcine nucleated cells; amplification of said DNA by polymerase chain reaction using oligonucleotides as primers that are complementary to the DNA sequence of the alpha (1,2) -fucosyltransferase gene 1; performing cutting with at least one restriction enzyme; separation of the obtained fragments using gel electrophoresis; determination of the corresponding number and lengths of fragments on the gel; and determining, based on the number and lengths of F18 fragments, which particular receptors are present.  14. The molecular analysis of claim 13, wherein the restriction enzyme is Cfol.  15. Set for. detecting E. coli F18 receptors, wherein said kit includes oligonucleotides that are in separate containers and that are complementary to a DNA sequence with polymorphism of the porcine alpha (1,2) -fucosyl transferase gene 1.  16. A porcine polypeptide having alpha (1,2) -fucosyltransferase activity and an amino acid substitution at position 103.  17. The polypeptide according to p. 16, in addition, characterized in that it has an amino acid substitution at position 286.  18. The use of pig alpha-(1,2) -fucosyltransferase polymorphism for the development of drugs for the treatment of pigs with E. coli-associated disease.