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RU94044956A - Method of preparing antigen for making diagnosticum from acetone-dried cells mycobacterium tuberculosis - Google Patents

Method of preparing antigen for making diagnosticum from acetone-dried cells mycobacterium tuberculosis

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Publication number
RU94044956A
RU94044956A RU94044956/13A RU94044956A RU94044956A RU 94044956 A RU94044956 A RU 94044956A RU 94044956/13 A RU94044956/13 A RU 94044956/13A RU 94044956 A RU94044956 A RU 94044956A RU 94044956 A RU94044956 A RU 94044956A
Authority
RU
Russia
Prior art keywords
acetone
diagnosticum
dried cells
making
mycobacterium tuberculosis
Prior art date
Application number
RU94044956/13A
Other languages
Russian (ru)
Other versions
RU2095816C1 (en
Inventor
А.А. Воробьев
Н.М. Гизатулина
В.С. Хлебников
И.Р. Головлев
Л.И. Краснопрошина
Н.И. Фадеева
С.С. Афанасьев
В.В. Гусев
Original Assignee
Московская медицинская академия им.И.М.Сеченова
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Московская медицинская академия им.И.М.Сеченова filed Critical Московская медицинская академия им.И.М.Сеченова
Priority to RU94044956A priority Critical patent/RU2095816C1/en
Publication of RU94044956A publication Critical patent/RU94044956A/en
Application granted granted Critical
Publication of RU2095816C1 publication Critical patent/RU2095816C1/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

FIELD: microbiology, immunology, immunochemistry. SUBSTANCE: method involves mechanical extraction of acetone-dried cells, centrifugation, gel-chromatography on Sephacryl S-200, concentration on diaphragm Amicon, dialysis and lyophilization. EFFECT: enhanced sensitivity and specificity of obtained antigen preparation.

Claims (1)

Изобретение относится к иммунологии и иммунохимии и касается способа получения диагностикума для серодиагностики туберкулеза, а также может использоваться для постановки туберкулиновых проб. Цель - получение более чувствительного и специфичного антигенного препарата достигается механической экстракцией ацетонвысушенных клеток, центрифугированием, гель-хроматографией на сефакрил S-200, концентрацией на мембране Amicon, диализом, лиофилизацией.The invention relates to immunology and immunochemistry and relates to a method for producing a diagnosticum for serodiagnosis of tuberculosis, and can also be used for formulation of tuberculin samples. The goal - obtaining a more sensitive and specific antigenic preparation is achieved by mechanical extraction of acetone-dried cells, centrifugation, gel chromatography on Sephacryl S-200, concentration on the Amicon membrane, dialysis, lyophilization.
RU94044956A 1994-12-22 1994-12-22 Method of preparing antigen from mycobacterium tuberculosis cells for diagnosticum construction RU2095816C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94044956A RU2095816C1 (en) 1994-12-22 1994-12-22 Method of preparing antigen from mycobacterium tuberculosis cells for diagnosticum construction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94044956A RU2095816C1 (en) 1994-12-22 1994-12-22 Method of preparing antigen from mycobacterium tuberculosis cells for diagnosticum construction

Publications (2)

Publication Number Publication Date
RU94044956A true RU94044956A (en) 1996-10-10
RU2095816C1 RU2095816C1 (en) 1997-11-10

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU94044956A RU2095816C1 (en) 1994-12-22 1994-12-22 Method of preparing antigen from mycobacterium tuberculosis cells for diagnosticum construction

Country Status (1)

Country Link
RU (1) RU2095816C1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10910934B2 (en) 2015-10-15 2021-02-02 Vastech Holdings Ltd. Electric motor

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2141665C1 (en) * 1998-04-14 1999-11-20 Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ Method of detection of tuberculosis mycobacterium
RU2212667C2 (en) * 2000-04-19 2003-09-20 Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии Method for diagnosing disseminated pulmonary tuberculosis with marked specific cellular immunity inhibition

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10910934B2 (en) 2015-10-15 2021-02-02 Vastech Holdings Ltd. Electric motor

Also Published As

Publication number Publication date
RU2095816C1 (en) 1997-11-10

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