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RU94028933A - Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-gg(t/g)(ag)cc-3' - Google Patents

Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting the nucleotide sequence 5'-gg(t/g)(ag)cc-3'

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Publication number
RU94028933A
RU94028933A RU94028933/13A RU94028933A RU94028933A RU 94028933 A RU94028933 A RU 94028933A RU 94028933/13 A RU94028933/13 A RU 94028933/13A RU 94028933 A RU94028933 A RU 94028933A RU 94028933 A RU94028933 A RU 94028933A
Authority
RU
Russia
Prior art keywords
enzyme
pph14
producer
nucleotide sequence
restrictase
Prior art date
Application number
RU94028933/13A
Other languages
Russian (ru)
Other versions
RU2110573C1 (en
Inventor
В.Е. Репин
Л.И. Пучкова
Э.К. Родичева
Г.А. Выдрякова
Original Assignee
Научно-производственное объединение "Вектор"
Институт биофизики СО РАН
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Научно-производственное объединение "Вектор", Институт биофизики СО РАН filed Critical Научно-производственное объединение "Вектор"
Priority to RU94028933A priority Critical patent/RU2110573C1/en
Publication of RU94028933A publication Critical patent/RU94028933A/en
Application granted granted Critical
Publication of RU2110573C1 publication Critical patent/RU2110573C1/en

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  • Enzymes And Modification Thereof (AREA)

Abstract

FIELD: molecular biology, genetic engineering, biotechnology. SUBSTANCE: invention proposes new strain Photobacterium phosphoreum (VKPM B-6667) - a producer of restriction endonuclease Pph14 I isolated from sea water after restriction endonucleases screening. Enzyme yield is 10000 U/g wet biomass, specific activity of the end enzyme is 20000 U/ml. Restrictase Pph14 I is an isoschizomer of restrictase HgiH I and can replace the latter in all genetic engineering works. Restrictase Pph14 I is thermostable enzyme that recognizes and splits the nucleotide sequence 5'-GG(T/C)(A/G)CC-3'. EFFECT: new strain indicated above, high yield and activity of enzyme. 10 cl, 1 dwg

Claims (1)

Применение: микробиология и биотехнология. Техническая задача: выявление штамма-продуцента, обеспечивающего получение термостабильной рестриктазы, узнающей и расщепляющей нуклеотидную поледовательность 5,-GG(T/C)(A/G)CC-3,, с высоким выходом и активностью фермента. Предложен новый штамм Photobacterium phosphoreum (ВКПМ В-6667) - продуцент рестриктазы Pph14 I, выделенный из морской воды в результате поиска продуцентов рестриктаз. Положительный эффект: выход фермента составляет 10000 ед/г сырой биомассы, удельная активность целевого фермента составляет 20000 ед/мл. Рестриктаза Pph14 I является изошизомером рестриктазы Hgi H I и может заменить последнюю во всех генно-инженерных работах.Application: microbiology and biotechnology. Technical problem: detection-producing strain provides a thermostable restriction enzyme recognizes and cleaves the nucleotide poledovatelnost 5, -GG (T / C) ( A / G) CC- 3 with a high yield and the activity of the enzyme. A new strain of Photobacterium phosphoreum (VKPM B-6667) is proposed - the producer of the restriction enzyme Pph14 I isolated from sea water as a result of the search for the producers of restrictase enzymes. Positive effect: the enzyme yield is 10,000 units / g of crude biomass, the specific activity of the target enzyme is 20,000 units / ml. The restriction enzyme Pph14 I is an isoschisomer of the Hgi HI restriction enzyme and can replace the latter in all genetic engineering studies.
RU94028933A 1994-08-02 1994-08-02 Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting nucleotide sequence 5'-gg(t/c)(ag)cc-3' RU2110573C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94028933A RU2110573C1 (en) 1994-08-02 1994-08-02 Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting nucleotide sequence 5'-gg(t/c)(ag)cc-3'

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94028933A RU2110573C1 (en) 1994-08-02 1994-08-02 Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting nucleotide sequence 5'-gg(t/c)(ag)cc-3'

Publications (2)

Publication Number Publication Date
RU94028933A true RU94028933A (en) 1997-03-10
RU2110573C1 RU2110573C1 (en) 1998-05-10

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Family Applications (1)

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RU94028933A RU2110573C1 (en) 1994-08-02 1994-08-02 Strain of bacterium photobacterium phosphoreum - a producer of restriction endonuclease recognizing and splitting nucleotide sequence 5'-gg(t/c)(ag)cc-3'

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Publication number Publication date
RU2110573C1 (en) 1998-05-10

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