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RU93013954A - METHOD FOR DETECTING THE VIRUS OF IMMUNODEFICIENCY - Google Patents

METHOD FOR DETECTING THE VIRUS OF IMMUNODEFICIENCY

Info

Publication number
RU93013954A
RU93013954A RU93013954/13A RU93013954A RU93013954A RU 93013954 A RU93013954 A RU 93013954A RU 93013954/13 A RU93013954/13 A RU 93013954/13A RU 93013954 A RU93013954 A RU 93013954A RU 93013954 A RU93013954 A RU 93013954A
Authority
RU
Russia
Prior art keywords
virus
author
primers
fragment
probe
Prior art date
Application number
RU93013954/13A
Other languages
Russian (ru)
Other versions
RU2039827C1 (en
Inventor
Н.В. Макаров
А.А. Елов
Original Assignee
Научно-производственное предприятие "Биотехинвест"
Filing date
Publication date
Application filed by Научно-производственное предприятие "Биотехинвест" filed Critical Научно-производственное предприятие "Биотехинвест"
Priority to RU93013954A priority Critical patent/RU2039827C1/en
Priority claimed from RU93013954A external-priority patent/RU2039827C1/en
Application granted granted Critical
Publication of RU2039827C1 publication Critical patent/RU2039827C1/en
Publication of RU93013954A publication Critical patent/RU93013954A/en

Links

Claims (1)

Использование: молекулярная биология, диагностика СПИДа. Сущность: способ обнаружения вируса иммунодефицита человека заключается в том, что проводят амплификацию ДНК вируса с помощью праймеров у 26 звеньев со следующей последовательностью нуклеотидов: GATGGCCCAAAAGTTAAACAATGGCC и праймер из 31-го звена со следующей последовательностью нуклеотидов: TTCTCTTATTAAGTTCTCTGAAATCTACTA, обнаруживают размноженный фрагмент в результате гибридизации с меченым зондом из 36 звеньев CTCCATTTAGTACTGTCTTTTTTCTTTATGGCAAAT и зондом на носителе из 32 звеньев ATTTCTACTAATGCTTTTATTTTTTCTTCTG. Одновременно амплификацию ДНК вируса осуществляют с использованием в качестве праймеров СН 26 и СН 27. В данном случае гель после автора диографии прокрашивают бромистым этидием, при этом вирус выявляют лишь в случае, если в прокрашенном геле присутствует фрагмент маркерного гена.Usage: molecular biology, AIDS diagnosis. The inventive method for detecting human immunodeficiency virus is that amplify viral DNA using primers in 26 units with the following nucleotide sequence: GATGGCCCAAAAGTTAAACAATGGCC and primer 31-th link with the following nucleotide sequence: TTCTCTTATTAAGTTCTCTGAAATCTACTA, exhibit propagated fragment by hybridization with a labeled a probe of 36 links CTCCATTTAGTACTGTCTTTTTTCTTTATGGCAAAT and a probe on a carrier of 32 links ATTTCTACTAATGCTTTATTTTTTCTTCTG. At the same time, the virus DNA amplification is carried out using CH 26 and CH 27 as primers. In this case, the author is stained with ethidium bromide after the author, and the virus is detected only if a fragment of the marker gene is present in the stained gel.
RU93013954A 1993-03-17 1993-03-17 Method of human immunodeficiency virus detection RU2039827C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93013954A RU2039827C1 (en) 1993-03-17 1993-03-17 Method of human immunodeficiency virus detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93013954A RU2039827C1 (en) 1993-03-17 1993-03-17 Method of human immunodeficiency virus detection

Publications (2)

Publication Number Publication Date
RU2039827C1 RU2039827C1 (en) 1995-07-20
RU93013954A true RU93013954A (en) 1995-10-20

Family

ID=20138779

Family Applications (1)

Application Number Title Priority Date Filing Date
RU93013954A RU2039827C1 (en) 1993-03-17 1993-03-17 Method of human immunodeficiency virus detection

Country Status (1)

Country Link
RU (1) RU2039827C1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2125089C1 (en) * 1996-08-23 1999-01-20 Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Method and set for detection of african plague pig virus dna by method of polymerase chain reaction

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