RU2807468C1 - Method for producing dry antiparasitic drug based on basidiomycete cantharellus cibarius - Google Patents

Abstract

FIELD: biotechnological; pharmaceutical industries.

SUBSTANCE: invention is related to methods for producing a dry antiparasitic drug. The method for producing a dry antiparasitic drug based on the basidiomycete Cantharellus cibarius involves drying the first component of the drug obtained by extraction with water, after which it is mixed with the second component of the drug. Drying of the first component, which is an aqueous extract of the basidiomycete Cantharellus cibarius, is carried out in a freeze dryer with a temperature in a vacuum chamber in the range from -35 to -25°C on the shelf heated to a temperature in the range from 10 to 25°C. Drying of the second component, which is the fruiting body of the basidiomycete Cantharellus cibarius, is carried out on a shelf dryer in the temperature range from 35 to 40°C, followed by crushing. After this, the components are mixed in a ratio of 1:3 to 1:5.

EFFECT: proposed method for producing a dry antiparasitic drug based on the basidiomycete Cantharellus cibarius provides an expansion of the arsenal of antiparasitic agents with high antioxidant activity.

1 cl, 3 tbl, 3 ex

Description

The invention relates to the biotechnological and pharmaceutical industries, in particular to methods for producing a dry antiparasitic drug.

A method of obtaining a dry extract of Phallus impudicus is known from the prior art, including extracting the mycelium of Phallus impudicus with water, followed by drying the aqueous extract, and after extracting the mycelium of Phallus impudicus with water, the aqueous extract is additionally filtered, followed by spray drying of the filtered aqueous extract. [RU2784058, A23L 31/15, publ. 10/04/2022].

The technical result is the production of a dry extract with a relative humidity of 2.04 - 4.1%, which has antioxidant activity and a long shelf life.

Closest to the claimed invention is a method for obtaining a remedy for the prevention and treatment of metabolic syndrome and diabetic nephropathy, characterized in that pre-dry extracts of hawthorn fruits and motherwort herbs are obtained by extraction with an aqueous or aqueous-ethanol solvent, by maceration, or percolation, filtration extraction, or repercolation followed by drying of the dry extract, after which they are mixed with taurine and auxiliary substances that act as binders, disintegration agents, and materials that promote sliding and lubrication of the granulate during pressing [RU 2519744, A61K 36/734, A61K 36/533 , A61P 3/10, A61P 3/06, publ. 06/20/2014 - prototype].

Despite the difference in purpose between the product obtained by the method described above and the drug according to the claimed invention, some features of the above-described technical solution, characterizing individual technological operations, can also be used in the proposed method.

The objective of the invention is to expand the arsenal of methods for producing dry antiparasitic drugs.

The technical result is the production of a dry antiparasitic drug with a relative humidity of 4%, which has antioxidant activity.

The technical result is achieved by the fact that in the method for producing a dry antiparasitic drug based on the basidiomycete Cantharellus cibarius, the first component of the drug, obtained by extraction with water, is dried, after which it is mixed with the second component of the drug, while the first component, which is an aqueous extract of the basidiomycete, is dried. mushroom Сantharellus cibarius, in a freeze dryer with a temperature in a vacuum chamber in the range from -35 ° C to -25 ° C and heating the shelf to a temperature in the range from 10 ° C to 25 ° C, after which it is mixed with pre-crushed and dried shelf dryer in the temperature range from 35 °C to 40 °C with the second component, which is the fruiting body of the basidiomycete fungus Cantharellus cibarius, in a ratio of 1:3 to 1:5.

The basidiomycete mushroom Cantharellus cibarius contains polysaccharides, in particular beta-glucans, which have antioxidant and antiparasitic effects. Once in the human body, they activate eosinophils. Mature human eosinophils have a nucleus divided into two parts (bipartite) and eosinophilic granules containing proteins with cytotoxic properties against multicellular parasites. In particular, such proteins are embedded in the membranes of their cells, disrupting their integrity of helminths.

The invention is illustrated by the following embodiments

Example 1. An aqueous extract of Cantharellus cibarius was dried in a freeze dryer at a temperature in a vacuum chamber
-35 °C and heating the shelf to 10 °C. The resulting dry extract of Cantharellus cibarius was a dry powder of gray-orange color. Next, the dry extract of Cantharellus cibarius was mixed with crushed dry fruit body of Cantharellus cibarius, dried in a shelf dryer at a temperature of 35 °C. The ratio of dry extract of Cantharellus cibarius and crushed dry fruiting body of Cantharellus cibarius was 1:3.

Example 2. An aqueous extract of Cantharellus cibarius was dried in a freeze-dryer (lyophiol) with a vacuum chamber temperature of -25 °C and a shelf heated to 15 °C. The resulting dry extract of Cantharellus cibarius was a gray-orange dry powder. Next, the dry extract of Cantharellus cibarius was mixed with crushed dry fruit body of Cantharellus cibarius, dried in a shelf dryer at a temperature of 37 °C. The ratio of dry extract of Cantharellus cibarius and crushed dry fruiting body of Cantharellus cibarius was 1:4.

Example 3. An aqueous extract of Cantharellus cibarius was dried in a freeze-dryer with a vacuum chamber temperature of -30 °C and a shelf heated to 25 °C. The resulting dry extract of Cantharellus cibarius was a gray-orange dry powder. Next, the dry extract of Cantharellus cibarius was mixed with crushed dry fruit body of Cantharellus cibarius, dried in a shelf dryer at a temperature of 40 °C. The ratio of dry extract of Cantharellus cibarius and crushed dry fruiting body of Cantharellus cibarius was 1:5.

In each of the above examples, drying the aqueous extract of Cantharellus cibarius in a freeze dryer was carried out as follows. The aqueous extract was poured into trays and placed on a shelf in a vacuum chamber of a freeze dryer, in which the temperature was maintained within a given range. Then the aqueous extract was frozen for 7 hours, after which a given pressure was created in a vacuum chamber and the shelf was heated in a given temperature range. The sublimation stage was 15 hours.

The technological process modes for freeze-drying an aqueous extract of Cantharellus cibarius in a freeze-dryer LZ-45 Frigera (made in the Czech Republic) according to the examples described above are shown in Table 1.

Table 1 Example No. Temperature
sublimation, °C / chamber pressure, Pa average temperature
on a surface
desublimator, °C Duration
drying, h Intensity
dehumidification at the stage
sublimation, kg/ (h⋅m ² ) Specific thermal
load, kW/m ²
(% of maximum
load) General Stage
Sublimation 1 -35/38 -48 22 15 0.59 0.46 (36.5) 2 -25/38 -38 22 15 0.56 0.42(35.7) 3 -30/38 -43 22 15 0.58 0.44(36.1)

The results of repeated experiments in accordance with the above examples were processed using the Statistica program, which is a software package for statistical analysis developed by StatSoft, at P = 0.95, n = 15, where P is the reliability coefficient of the experiments; n is the number of experiments performed. Antioxidant activity was measured by the coulometric method, and relative humidity by the method of drying and weighing the dry residue. The results of the experiments are shown in Tables 2, 3.

table 2 Antioxidant activity and relative humidity of dry extracts
Сantharellus cibarius after freeze-drying Example Shelf temperature, °C Antioxidant activity, Coulomb/g Relative humidity, % 1 10 520 4 2 15 480 3 3 25 410 2

Table 3 Antioxidant activity and relative humidity of dry antiparasitic drug Сantharellus cibarius Example Ratio of dry extract of Cantharellus cibarius and crushed dry fruiting body of Cantharellus cibarius Antioxidant activity, Coulomb/g Relative humidity, % 1 1:3 450 4 2 1:4 410 4 3 1:5 390 4

Obtaining a dry preparation with a relative humidity of 4% allows for the preservation of the antioxidant properties of the preparation.

The dry preparation obtained according to examples 1-3 exhibited antiparasitic properties in in vitro experiments on a biological model using nematodes in the drug concentration range of 20-100 μg/ml.

Thus, drying the first component, which is an aqueous extract of the basidiomycete Cantharellus cibarius, in a freeze dryer with a temperature in a vacuum chamber in the range from -35 °C to -25 °C and heating the shelf to a temperature in the range from 10 °C to 25 °C, followed by mixing it with the second component, which is the crushed fruiting body of the basidiomycete mushroom Cantharellus cibarius, pre-crushed and dried on a shelf dryer in the temperature range from 35 °C to 40 °C, in a ratio of 1:3 to 1:5 allows implement the claimed invention with the achievement of the specified technical result.

Claims (1)

A method for producing a dry antiparasitic drug based on the basidiomycete Cantharellus cibarius, in which the first component of the drug obtained by extraction with water is dried, after which it is mixed with the second component of the drug, characterized in that the first component, which is an aqueous extract of the basidiomycete Cantharellus, is dried cibarius, in a freeze dryer with a temperature in a vacuum chamber in the range from -35 to -25 °C and heating the shelf to a temperature in the range from 10 to 25 °C, after which it is mixed with pre-crushed and dried on a shelf dryer in the temperature range from 35 to 40 °C with the second component, which is the fruiting body of the basidiomycete fungus Cantharellus cibarius, in a ratio of 1:3 to 1:5.