RU2806550C1 - Strains and methods of obtaining protein of single-cellular organisms or biomass - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: isolated bacterial strain of Xanthobacter genus is proposed, the said strain is deposited under number VTT-E-193585 and is suitable for use in feed and food products. A culture containing the specified strain and suitable for use in feed and food products is also proposed. Also the following is proposed: a method of obtaining biomass of the specified strain and a method of obtaining protein, which involves obtaining biomass, including cultivating the specified strain.

EFFECT: invention provides a high protein content in the cells of the specified strain.

16 cl, 2 dwg, 5 tbl, 4 ex

Description

FIELD OF TECHNOLOGY TO WHICH THE INVENTION RELATES

The present invention relates to the production of protein and/or other macromolecules using microorganisms. In particular, the present invention relates to new bacterial strains and methods of continuous cultivation for the production of protein or biomass using bacteria, in which gases and minerals are introduced into the cells. The present invention also relates to products of these methods and the use of these products, for example in food or feed.

BACKGROUND OF THE INVENTION

A growing world population, climate change and water scarcity increasingly pose a threat to traditional agriculture and therefore to an adequate supply of food and feed. Therefore, alternative sources of organic molecules such as proteins are being explored. A potential alternative is the production of single cells, that is, the production of protein and/or other macromolecules using microorganisms.

Chemoautotrophic microorganisms are described that are capable of growing on a minimal mineral medium with hydrogen gas as an energy source and carbon dioxide as the only carbon source. For a review of these microorganisms, see, for example, Shively et al. (1998) Annu Rev Microbiol 52:191. International Patent Application WO2018144965 describes various microorganisms and bioprocesses for converting gaseous substrates into high-protein biomass. The work of Andersen et al. (1979) Biochim Biophys Acta 585:1-11 describe mutant strains of Alcaligenes eutrophus , a hydrogen bacterium that grows readily in heterotrophic and autotrophic conditions. Mutants with altered ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activity have been characterized. The work of Ohmiya et al. (2003) J. Biosci. Bioeng. 95:549-561 describes the use of microbial genes for the utilization of non-degradable biomass. In the work of Yu Jian et al. (2013) Int J Hydrogen Ener 38:8683–8690 described carbon dioxide fixation by a hydrogen-oxidizing bacterial isolate. A high energy efficiency of 50% was measured at a moderate oxygen concentration (10 mol%).

However, different chemoautotrophic microorganisms have different properties in terms of growth rate, yield, biomass composition, as well as properties associated with use as a food ingredient, such as safety for human consumption, taste, smell, mouthfeel, technical and functional properties in cooking, etc. Not every chemoautotrophic microorganism has a sufficient growth rate and provides sufficient yield, and not every method can be realistically scaled up to an economically viable large-scale process. To have sufficient yield of functional protein, i.e. for food or feed, it is important to find a suitable production organism and a suitable process that can be carried out on a large scale. This need is addressed by the present invention.

SUMMARY OF THE INVENTION

In a first main aspect, the present invention relates to the isolated bacterial strain VTT-E-193585 or a derivative thereof.

In additional aspects, the present invention relates to a culture containing a bacterial strain of the present invention or a derivative thereof. In addition, the present invention relates to a method for producing biomass and/or protein, where the method includes cultivating a bacterial strain of the present invention or a derivative thereof.

In yet another aspect, the present invention relates to a method for producing biomass and/or protein, wherein the method includes cultivating a bacterial strain of the genus Xanthobacter in a continuous culture with hydrogen as an energy source and a source of inorganic carbon, wherein the inorganic carbon source includes carbon dioxide .

In additional basic aspects, the present invention relates to bulk protein, biomass, or non-protein cellular or chemical components produced or produced by the process of the present invention, and to a food or feed product produced or produced by the method of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

Fig. 1 . Optical density measured at a wavelength of 600 nm (black circles) and optical density sensor readings during chemoautotrophic cultivation in a volume of 200 liters of the isolated bacterial strain, deposited as VTT-E-193585.

Fig. 2 . Optical density measured at a wavelength of 600 nm during parallel chemoautotrophic cultivation in a volume of 200 ml of the isolated bacterial strain deposited as VTT-E-193585 on various nitrogen sources.

DETAILED DESCRIPTION OF THE INVENTION

Definitions

As used herein, the term “isolated,” for example, in the context of a strain means isolated from its natural environment. Preferably, the isolated strain is pure, i.e. free from other strains.

The term "derivative" as used herein in the context of a strain refers to a strain that is derived from a reference strain, i.e. created using a reference strain as a starting point. For example, a genetically modified or otherwise mutated or genetically modified strain is an embodiment of such a derivative. Genetic modifications include point mutations and insertions or deletions, including insertions or deletions of entire loci or fragments thereof. The derivative preferably has less than 10 genetic modifications, i.e. less than 5, for example 4, 3, 2 or 1 genetic modifications compared to the reference strain.

As used herein, the term “culture” refers to a suspension of viable cells in a liquid medium.

The term "biomass" has its usual meaning in the field of bacterial fermentation and refers to cellular material.

As used herein, the term "continuous culture" refers to a culture process in which fresh media is continuously added to the culture and bacterial culture media is continuously removed at substantially the same rate.

Aspects and Embodiments of the Present Invention

In a first main aspect, the present invention relates to the isolated bacterial strain VTT-E-193585 or a derivative thereof.

Strain VTT-E-193585 was isolated from the Baltic Sea coast in Naantali, Finland. This organism is capable of growing in suitable bioreactor conditions with a minimal mineral environment with hydrogen as an energy source and carbon dioxide as a carbon source under oxygen-limited conditions. 16S sequencing and Illumina metagenomic sequencing indicated that the strain most likely belongs to the genus Xanthobacter , but is not a known species. The bacterial strain is very suitable for food and feed because the dried cell powder has a high protein content and contains all the essential amino acids. It also contains more unsaturated fatty acids than saturated fatty acids and high levels of B vitamins. It has low levels of peptidoglycans and lipopolysaccharides, which can cause allergies or toxicity. A toxicity assay was performed and no genotoxicity or cytotoxicity was observed for the strain. In addition, the strain is generally sensitive to antibiotics.

Strain VTT-E-193585 (SoF1) was deposited on 11 June 2019 in the VTT Culture Collection at the VTT Technical Research Center in Finland, P.O. Box 1000, FI-02044 VTT, Finland, International Depository Authority under the Treaty of Budapest. Additional information regarding the characteristics of the strain and methods for culturing the strain are provided in the Examples provided herein.

In a preferred embodiment, if the strain is a derivative of strain VTT-E-193585, the derivative retains the ability to grow using hydrogen gas as an energy source and carbon dioxide as the sole carbon source.

In one embodiment of the invention, if the strain is a derivative of strain VTT-E-193585, then this derivative contains 16S ribosomal RNA specified in SEQ ID NO:1, or 16S ribosomal RNA having up to 20 nucleotide differences from SEQ ID NO:1, eg 1 to 10, eg 1 to 5, eg one, two or three nucleotide differences of SEQ ID NO:1.

SEQ ID NO:1. Sequence of 16S ribosomal RNA of strain VTT-E-193585:

CTTGAGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGCCCAGCAATGGGAGCGGCAGACGGGTGAGTAACGCGTGGGGATGTGCCCAATGGTACGGAATAACCCAGGGAAACTTGGACTAATACCGTATGAGCCCTTCGGGGGAAAGATTTATCGCCATTGGATCAACCCGCGTCTGATTAGCTAGTTGGTGGGGTAACGGCCCACCAAGGCG ACGATCAGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTGTGATGAAGGCCTTAGGGTTGTAAAGCACTTTCGCCGGTGAAGATAATGACGGTAACCGGAGAAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCT AGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGATCGTTAAGTCAGGGGTGAAATCCTGGAGCTCAACTCCAGAACTGCCCTTGATACTGGCGACCTTGAGTTCGAGAGAGGTTGGTGGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAGTGGCGAAGGCGGCCAACTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAG ATACCCTGGTAGTCCACGCCGTAAACGATGGATGCTAGCCGTTGGGCAGCTTGCTGTTCAGTGGCGCAGCTAACGCATTAAGCATCCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCCTTTGACATGGCAGGACGATTTCCAGAGATGGATCTCTTCCAGCAAT GGACCTGCACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCTCTAGTTGCCAGCATTCAGTTGGGCACTCTAGAGGGACTGCCGGTGATAAGCCGAGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTACGGGCTGGGCTACACACGTGCTACAATGGTGGTGACAGTGGGATGCGAAAGGGCGACC TCTAGCAAATCTCCAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGCTTTACCCGAAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCACGGTAGGGTCAGCGACTGGGGTGAAGTCGTAACAAGGTAGCCGTA GGGGAACCTGCGGCTGGATCACCTCCTTT

In another aspect, the present invention relates to a culture containing a bacterial strain of the present invention or a derivative thereof. In a preferred embodiment of the invention, the culture volume is 100 ml or more, for example 1 L or more, for example 10 L or more, for example 1000 L or more, for example 10,000 L or more, for example 50,000 L or more, for example 100,000 L or more, for example 200,000 l or more.

In yet another aspect, the present invention relates to a method for producing biomass and/or protein, wherein the method includes cultivating a bacterial strain of the present invention or a derivative thereof. In one embodiment of the invention, the method is for the production of biomass. In another embodiment of the invention, the method is for producing protein. In one embodiment of the invention, the method includes cultivating the strain in a continuous culture with hydrogen as an energy source and an inorganic carbon source, wherein the inorganic carbon source includes carbon dioxide. In a further embodiment of the invention, the method is for producing biomass and includes cultivating the strain in a continuous culture with hydrogen as an energy source and an inorganic carbon source, wherein the inorganic carbon source includes carbon dioxide. Various additional embodiments of the method are described below.

In another basic aspect, the present invention relates to a method for producing biomass and/or protein, wherein the method comprises cultivating a bacterial strain of the genus Xanthobacter in a continuous culture with hydrogen as an energy source and a source of inorganic carbon, wherein the inorganic carbon source includes dioxide carbon. In one embodiment of the invention, the method is for the production of biomass. In another embodiment of the invention, the method is for producing protein. Various additional embodiments of the method are described below.

Based on the genome sequence, the strain deposited as VTT-E-193585 most likely uses the Calvin-Benson-Bassham cycle for carbon fixation, in which a carbon dioxide molecule combines with a 5-carbon chain of ribulose-1,5-bisphosphate to form two molecules of glycerate-3-phosphate. This allows the strain to synthesize all the other organic molecules needed for growth. Hydrogen energy enters the cell most likely through NAD ⁺ -reducing hydrogenases and/or NiFeSe hydrogenases. Essentially, it is a redox reaction in which hydrogen (H ₂ ) is oxidized to H ⁺ and NAD ⁺ is reduced to NADH. In addition to ATP, NADH is one of the main energy carriers within living organisms. Alternatively, some other energy equivalent is reduced by another hydrogenase enzyme using H ₂ . The Calvin-Benson-Bassham cycle requires energy in the form of ATP and NADH/NADPH to bind CO ₂ . The strain most likely generates ATP through oxidative phosphorylation, which consists of four protein complexes that create a proton gradient across the membrane. The proton gradient is generated mainly by the energy of NADH. The proton gradient drives the ATP synthase complex that generates ATP. Judging by the genome sequence, the strain has an F-type bacterial ATP synthase.

It should be understood that when a method is stated to involve cultivating a strain with an inorganic carbon source, the inorganic carbon source is the primary carbon source in the culture. Thus, trace amounts of organic carbon sources may be present in the crop, but the major metabolism and growth of the crop is based on the use of an inorganic carbon source, preferably carbon dioxide, as the carbon source. Preferably, the proportion of carbon supplied to the crop that is organic is less than 5%, for example less than 1%, for example less than 0.1% of the total carbon supplied to the crop during the process. Preferably, no sources of organic carbon are supplied during the implementation of the method.

Likewise, it should be understood that when a method is stated to involve cultivating a strain with hydrogen (H ₂ ) as an energy source, then hydrogen is the primary energy source in the culture. Thus, other minor energy sources may be present in the crop, such as ammonia, which can be a source of nitrogen, or small amounts of organic compounds, but the major metabolism and growth of the crop is based on the use of hydrogen as an energy source. In the general process, hydrogen is preferably produced by electrolysis of water; those. by splitting water into hydrogen and oxygen gases with electricity. Thus, hydrogen and oxygen gases are supplied to the bioreactor from a nearby electrolyzer. Alternatively, electrodes can be placed inside a bioreactor to produce hydrogen and oxygen in the bioreactor rather than in a separate electrolyser.

The inorganic carbon source containing carbon dioxide may include other inorganic carbon sources such as, for example, carbon monoxide. In one embodiment of the invention, only carbon sources in gaseous form are supplied to the crop. In a preferred embodiment of the invention, carbon dioxide is the only source of inorganic carbon and, indeed, the only source of carbon supplied to the crop. In one embodiment of the invention, only gases and minerals are supplied to the crop, and the level of carbon dioxide in the supplied gas is from 10% to 50%, i.e. from 15% to 45%, for example from 20% to 40%, for example from 25% to 35%, for example from 26% to 30%.

In another embodiment of the invention, gases and minerals are supplied to the crop and the level of hydrogen (H ₂ ) in the supplied gas is from 30% to 80%, for example from 35% to 75%, for example from 40% to 70%, for example from 45% up to 65%, for example from 50% to 60%.

In another embodiment of the invention, gases and minerals are supplied to the crop, and the level of oxygen (O ₂ ) in the supplied gas is from 10% to 25%, for example from 15% to 20%, for example from 16% to 18%. In another embodiment of the invention, the level of oxygen supplied is such that the dissolved oxygen level in the culture is maintained at 5% to 10%.

In a preferred embodiment of the invention, only gases and minerals are supplied to the crop, and the supplied gas contains H ₂ , CO ₂ and O ₂ , where the percentage of H ₂ is from 40% to 70%, the percentage of CO ₂ is from 18% to 28% and the percentage of O ₂ is from 12% to 22%.

Typically, the method of the present invention involves adding a nitrogen source. The nitrogen source may, for example, be in the form of ammonium hydroxide, an ammonium salt such as ammonium sulfate or ammonium chloride, ammonia, urea or a nitrate such as potassium nitrate. In other embodiments of the invention, nitrogen gas (N ₂ ) is used as the nitrogen source. In a preferred embodiment of the invention, the nitrogen source is ammonium hydroxide or an ammonium salt such as ammonium sulfate.

In one embodiment of the invention, the supplied nitrogen source is ammonium hydroxide at a concentration of from 100 mg/L to 10 g/L, for example from 250 mg/L to 4 g/L, for example from 0.5 g/L to 2 g/ l, for example from 0.75 g/l to 1.5 g/l.

Typically, the method of the present invention involves the addition of minerals such as minerals containing ammonium, phosphate, potassium, sodium, vanadium, iron, sulfate, magnesium, calcium, molybdenum, manganese, boron, zinc, cobalt, selenium, iodine, copper and/or nickel. Suitable mineral media are well known in the art and, for example, have been described in Thermophilic Bacteria, CRC Press, Boca Raton, FL, Jacob K. Kristjansson, ed., 1992, for example, page 87, table 4.

In one embodiment of the invention, the added minerals include one or more of the following minerals: ammonia, ammonium (eg, ammonium chloride (NH ₄ Cl), ammonium sulfate ((NH ₄ ) ₂ SO ₄ )), nitrate (eg, potassium nitrate (KNO ₃ )), urea or organic nitrogen source; phosphate (e.g. disodium phosphate (Na ₂ HPO ₄ ), potassium phosphate (KH ₂ PO ₄ ), phosphoric acid (H ₃ PO ₄ ), potassium dithiophosphate (K ₃ PS ₂ O ₂ ), potassium orthophosphate (K ₃ PO ₄ ), disodium phosphate (Na ₂ HPO ₄ •2H ₂ O), dipotassium phosphate (K ₂ HPO ₄ ) or monopotassium phosphate (KH ₂ PO ₄ ); sulfate; yeast extract; chelated iron (for example, with EDTA or citric acid); potassium (for example, phosphate potassium (KH ₂ PO ₄ ), potassium nitrate (KNO ₃ ), potassium iodide (KI), potassium bromide (KBr)); and other inorganic salts, minerals and trace elements (for example, sodium chloride (NaCl), magnesium sulfate (MgSO ₄ •7H ₂ O) or magnesium chloride (MgCl ₂ ), calcium chloride (CaCl ₂ ), calcium sulfate (CaSO ₄ ) or calcium carbonate (CaCO ₃ ), manganese sulfate (MnSO ₄ •7H ₂ O) or manganese chloride (MnCl ₂ ), ferrous chloride (FeCl ₂ ), ferrous sulfate (FeSO ₄ •7H ₂ O) or ferric chloride (FeCl ₂ •4H ₂ O), sodium bicarbonate (NaHCO ₃ ) or sodium carbonate (Na ₂ CO ₃ ), zinc sulfate ( ZnSO ₄ ) or zinc chloride (ZnCl ₂ ), ammonium molybdate (NH ₄ MoO ₄ ) or sodium molybdate (Na ₂ MoO ₄ •2H ₂ O), copper sulfate (CuSO ₄ ) or copper chloride (CuCl ₂ •2H ₂ O) , cobalt chloride (CoCl ₂ •6H ₂ O) or cobalt sulfate (CoSO ₄ ), aluminum chloride (AlCl ₃ •6H ₂ O), lithium chloride (LiCl), boric acid (H ₃ BO ₃ ), nickel chloride (NiCl ₂ •6H ₂ O) or nickel sulfate (NiSO ₄ ), tin chloride (SnCl ₂ •H ₂ O), barium chloride (BaCl ₂ •2H ₂ O), copper selenate (CuSeO ₄ 5H ₂ O), sodium selenate (Na ₂ SeO ₄ ) or sodium selenite (Na ₂ SeO ₃ ), sodium metavanadate (NaVO ₃ ), chromium salts).

In a preferred embodiment, the method of the present invention includes adding one, more or all of: NH ₄ OH, KH ₂ PO ₄ , Na ₂ HPO ₄ •2H ₂ O, NaVO ₃ •H ₂ O, FeSO ₄ × 7H ₂ O , MgSO ₄ •7H ₂ O, CaSO ₄ , Na ₂ MoO ₄ •2H ₂ O, MnSO ₄ •7H ₂ O, ZnSO ₄ . •7H ₂ O, H ₃ BO ₃ , CoSO ₄ , CuSO ₄ , NiSO ₄ .

In one embodiment of the invention, the medium supplied to the cells contains less than 1 g/L chloride salts, such as less than 0.25 g/L chloride salts, such as less than 0.1 g/L chloride salts, such as less than 0.025 g/L chloride salts, for example less than 0.01 g/l chloride. In one embodiment of the invention, no chloride salts are added to the culture.

In another embodiment of the invention, no vitamins are added during the method, i.e. the medium supplied to the culture does not contain vitamins.

In another embodiment of the invention, no amino acids are added during the method, i.e. the medium supplied to the culture does not contain amino acids.

In another embodiment of the invention, no organic compounds are added during the process, i.e. the medium supplied to the culture does not contain any organic compounds.

In some embodiments of the invention, the pH of the bacterial culture is adjusted to a certain level. In some embodiments of the invention, the pH value is adjusted within the optimal range for the maintenance and/or growth of bacteria and/or the formation of organic compounds. In one embodiment of the invention, the pH of the culture is maintained in the range of 5.5 to 8.0, for example 6.5 to 7.0, for example 6.8.

In some embodiments, the temperature of the bacterial culture is controlled. In some embodiments of the invention, the temperature is controlled within an optimal range for the maintenance and/or growth of bacteria and/or the formation of organic compounds. In one embodiment of the invention, the crop is grown at a temperature of from 25°C to 40°C, for example from 28°C to 32°C, for example at 30°C.

Typically, the method of the invention is carried out in a bioreactor. A bioreactor is used to culture cells that can be maintained at specific phases of their growth curve. The use of bioreactors is beneficial in many respects for cultivation under chemoautotrophic growth conditions. Typically, a bioreactor facilitates control of growth conditions, including control of dissolved carbon dioxide, oxygen and other gases such as hydrogen, as well as other dissolved nutrients, trace elements, temperature and pH. Nutrient media, as well as gases, can be added to the bioreactor either as a one-time addition, or periodically, or in response to detected depletion, or at a given programmed point in time, or continuously during the growth and/or maintenance period of the culture. In a continuous cultivation process, nutrient media as well as gases are continuously added to the bioreactor. In addition, media containing bacteria are continuously removed from the bioreactor.

In a preferred embodiment of the invention, the volume of the bacterial culture is 100 ml or more, for example 1 L or more, for example 10 L or more, for example 100 L or more, for example 1000 L or more, for example 10000 L or more, for example 50000 L or more, for example, 100,000 l or more, for example, 200,000 l or more.

In one embodiment of the invention, the culture productivity is more than 0.1 g dry cell mass per liter per hour, for example more than 0.2, for example more than 0.3, for example more than 0.4, for example more than 0.5, for example more than 0.6 , for example more than 0.7, for example more than 0.8, for example more than 0.9, for example more than 1 g per liter per hour.

Bacteria can be inoculated directly from a cell bank or by seed culture on a smaller scale. It is desirable that the supply of fresh medium to the culture and the removal of spent medium with bacteria occur at the same rate, so that the volume in the bioreactor remains unchanged.

In one embodiment of the invention, after an initial phase of reaching a suitable cell density, the bacteria grow in a stationary or pseudo-stationary state, constantly remaining in their logarithmic phase at an OD600 above 5, for example above 10, for example above 20, for example from 50 to 200, for example from 50 to 100 .

In one embodiment of the method of the present invention, the bacterial strain has a growth rate of 0.04-0.12 h ^-1 .

In another embodiment of the method of the present invention, the liquid feed rate in the continuous phase is 50-80% of the growth rate.

Xanthobacter is a genus of Gram-negative bacteria in the family Xanthobacteraceae . In one embodiment, the Xanthobacter strain used in the method of the invention is a strain that uses the Calvin-Benson-Bassham pathway to convert carbon dioxide into organic compounds, such as glucose, needed for living organisms.

In one embodiment, the Xanthobacter strain used in the method of the present invention is a strain that uses NiFeSe hydrogenases to convert hydrogen (H ₂ ) into cellular energy equivalents.

In one embodiment, the Xanthobacter strain used in the method of the present invention is a strain that uses NAD ⁺ -reducing hydrogenases to convert hydrogen (H ₂ ) into cellular energy equivalents.

In one embodiment of the invention, the Xanthobacter strain used in the method of the present invention is capable of nitrogen fixation.

In one embodiment, the bacterial strain used in the method of the present invention is selected from the group consisting of: X. agilis , X. aminooxidans , X. autotrophicus , X. flavus , X. tagetidis , X. viscosus , Xanthobacter sp. 126, Xanthobacter sp. 91 and strain VTT-E-193585.

In a preferred embodiment, the bacterial strain used in the method of the present invention is VTT-E-193585 or X. tagetidis . Most preferably, the strain used in the method of the present invention is strain VTT-E-193585.

In another embodiment, the bacterial strain used in the method of the present invention contains 16S ribosomal RNA specified in SEQ ID NO:1, or 16S ribosomal RNA having up to 20 nucleotide differences from SEQ ID NO:1, for example from 1 to 10, for example from 1 to 5, for example one, two or three nucleotide differences from SEQ ID NO:1.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding a large chain ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) having the sequence specified in SEQ ID NO:3, or a sequence having more than 93 % identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98% identity, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:3.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding a small chain ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) having the sequence specified in SEQ ID NO:5, or a sequence having more than 83 % sequence identity, for example more than 86% identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98% identity, for example more than 99% sequence identity with the sequence shown in SEQ ID NO:5.

SEQ ID NO:2:

Nucleotide sequence of large chain ribulose bisphosphate carboxylase:

ATGGGTGCCGAAGCAACCGTCGGGCAGATCACGGACGCCAAGAAGAGATACGCCGCCGGCGTGCTGAAGTACGCCCAGATGGGCTACTGGAACGGCGACTACGTTCCCAAGGACACCGACCTCCTGGCGGTGTTCCGCATCACCCCCCAGGCGGGCGTGGACCCGGTGGAAGCCGCCGCGGCGGTCGCCGGCGAAAGCTCCACCGCTACCTGGACCGTGGTGTGGACCGACCGGCTCA CCGCCGCCGACGTCTACCGCGCCAAGGCCTACAAGGTGGAGCCGGTGCCGGGCCAGGAAGGCCAGTATTTCTGCTACATCGCCTATGATCTCGATTTGTTCGAGGAAGGCTCCATCGCCAACCTCACGGCGTCGATCATCGGCAACGTCTTCTCCTTCAAGCCGCTGAAGGCGGCGCGGCTGGAGGACATGCGGCTTCCCGTCGCCTATGTGAAGACCTTCCGCGGCCCGCCCACCGGCATCGTG GTCGAGCGCGAGCGCCTGGACAAGTTCGGCCGCCCCCTTCTGGGCGCCACCACCAAGCCGAAGCTTGGCCTCTCGGGCAAGAATTACGGCCGCGTGGTCTATGAGGCCCTCAAGGGCGGCCTCGACTTCGTGAAGGACGACGAGAACATCAACTCGCAGCCCTTCATGCACTGGCGCGATCGCTTCCTCTATTGCATGGAGGCCGTCAACAAGGCCCAGGCCGAGACCGGCGAGGTGAAGGGGCACT ATCTCAACATCACCGCCGGGACCATGGAGGAGATGTACCGCCGCGCCGAGTTCGCCAAGGAACTGGGCTCCGTGGTGGTGATGGTGGATCTCATCATCGGCTGGACCGCCATCCAGTCCATGTCCAACTGGTGCCGCGAGAACGACATGATCCTGCACATGCACCGTGCGGGCCATGGCACCTACACGCGCCAGAAGAGCCACGGCGTCTCCTTCCGCGTCATCGCCAAGTGGCTGCGGCTCGCC GGCGTCGACCACCTGCACACCGGCACCGCCGTGGGCAAGCTGGAAGGCGACCCCATGACCGTGCAGGGCTTCTACAATGTCTGCCGCGAGACGACGACGCAGCAGGACCTCACCCGCGGCCTGTTCTTCGAGCAGGACTGGGGGCGGCATCCGCAAGGTGATGCCGGTGGCCTCCGGCGGCATCCATGCGGGCCAGATGCACCAGCTCATCGACCTGTTCGGCGAGGACGTGGTGCTCCAGTT CGGCGGCGGCACCATCGGCCACCCGGACGGCATCCAGGCCGGCGCCACCGCCAACCGCGTGGCGCTGGAAACCATGATCCTCGCCCGCAACGAGGGCCGCGACATCAGGAACGAGGGCCCGGAAATCCTGGTGGAAGCCGCCAAATGGTGCCGTCCGCTGCGCGCGGCGCTCGATACCTGGGGCGAGGTGACCTTCAACTACGCCTCCACCGACACGTCCGATTACGTGCCCACCGCGTCCGTC GCCTGA

SEQ ID NO: 3:

Amino acid sequence of large chain ribulose bisphosphate carboxylase

MGAEATVGQITDAKKRYAAGVLKYAQMGYWNGDYVPKDTDLLAVFRITPQAGVDPVEAAAAVAGESSTATWTVVWTDRLTAADVYRAKAYKVEPVPGQEGQYFCYIAYDLDLFEEGSIANLTASIIGNVFSFKPLKAARLEDMRLPVAYVKTFRGPPTGIVVERERLDKFGRPLLGATTKPKLGLSGKNYGRVVYEALK GGLDFVKDDENINSQPFMHWRDRFLYCMEAVNKAQAETGEVKGHYLNITAGTMEEMYRRAEFAKELGSVVVMVDLIIGWTAIQSMSNWCRENDMILHMHRAGHGTYTRQKSHGVSFRVIAKWLRLAGVDHLHTGTAVGKLEGDPMTVQGFYNVCRETTTQQDLTRGLFFEQDWGGIRKVMPVASGGIHAGQMH QLIDLFGEDVVLQFGGGTIGHPDGIQAGATANRVALETMILARNEGRDIRNEGPEILVEAAKWCRPLRAALDTWGEVTFNYASTDTSDYVPTASVA

SEQ ID NO: 4:

Nucleotide sequence of small chain ribulose bisphosphate carboxylase:

ATGCGCATCACCCAAGGCTCCTTCTCCTTCCTGCCGGACCTCACCGACACGCAGATCAAGGCCCAGGTGCAATATTGCCTGGACCAGGGCTGGGCGGTCTCGGTGGAGCACACCGACGATCCCCACCCGCGCAACACCTATTGGGAGATGTGGGGCCCGCCCATGTTCGATCTGCGCGACGCGGCCCGGCGTCTTCGGCGAGATCGAAGCCTGCCGGGCCGCCAATCCCGAGCATTATGTG CGGGTGAACGCCTTCGATTCCAGCCGCGGATGGGAGACGATCCGCCTGTCCTTCATCGTTCAGCGGCCCACCGTGGAAGAGGGCTTCCGCCTCGACCGCACCGAAGGCAAGGGCCGCAACCAGAGCTACGCCATGCGCTACCGGGCGCAGTTCGCGCCGCGCTGA

SEQ ID NO: 5:

Amino acid sequence of small chain ribulose bisphosphate carboxylase:

MRITQGSFSFLPDLTDTQIKAQVQYCLDQGWAVSVEHTDDPHPRNTYWEMWGPPMFDLRDAAGVFGEIEACRAANPEHYVRVNAFDSSRGWETIRLSFIVQRPTVEEGFRLDRTEGKGRNQSYAMRYRAQFAPR

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the alpha subunit of NAD ⁺ -reducing hydrogenase HoxS having the sequence specified in SEQ ID NO: 7, or a sequence having more than 70% sequence identity, for example more than 80% identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:7.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the beta subunit of NAD ⁺ -reducing hydrogenase HoxS having the sequence specified in SEQ ID NO:9, or a sequence having more than 77% sequence identity, for example, more than 80% identity, for example more than 90% identity, for example, more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:9.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the gamma subunit of NAD ⁺ -reducing hydrogenase HoxS having the sequence specified in SEQ ID NO:11, or a sequence having more than 70% sequence identity, for example, more than 80% identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO :eleven.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the delta subunit of the NAD+ ^reducing hydrogenase HoxS having the sequence set forth in SEQ ID NO:13, or a sequence having greater than 79% sequence identity, for example more than 80% identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:13.

SEQ ID NO:6:

Nucleotide sequence of the alpha subunit of NAD+ ^- reducing hydrogenase HoxS:

ATGATGCCATCTGAGCCGCACGGCGCGGGCATGCCGCCCCCACGGGAAGCGGCCGCGGTTCCCACCCCCCAGGAGGTGAGCGCGGTGGTGGCCGAGGTGGTCGCGGATGCCGTGGCATCGGTGGGCGGCGCACGCACCCGGCTCATGGACATCGTCCAGCTGGCCCAGCAGCGTCTCGGCCATCTCTCCGAAGACCATGGCGGCCATTGCCGCGCGGCTCGCCATTCCGCCGGTGGAAG TGGCGGACATGGTGTCCTTCTACGCCTTCCTGAACCGCGCGCCCAAGGGCCGCTACCACATCCGCCTGTCGCGCAGCCCCATCTCGCTGATGAAGGGCGCCGAGGCGGTGGCTGCCGCCTTCTGCCAGATCCTCGGCATCGCCATGGGCGAGACCTCGCAGGATGGCGACTTCACCCTGGAATGGACCAACGACATCGGCATGGCCGACCAGGAGCCGGCCGCCCTCGTCAACGGCACGGTGATGACG CAGCTCGCGCCCGGCGATGCGGCCATCATCGTCGGCCGGCTGCGGGCCCATCACGCGCCCAATGCCCTGCCGCTGTTCCCTGGAGCCGGCGTGGCCGGCTCCGGCCTGCCCCATGCCCGGATCCGCCCCAGCCTGGTGATGCCGGGACAGCTTCTGTTCCGCGAGGACCACACGACGCCGGGCGCCGGCATCAAGGCGGCACTCGCCCTCACCCCGGACGAAGTGGTGCAGAAGGTCTCCGCCGCGC GCCTGCGCGGGCGGGGTGGCGCCGGCTTTCCCACCGGTCTCAAATGGAAGCTCTGCCGCCAGTCGCCCGCCACCACCCGCCATGTGATCTGCAATGCGGACGAGGGCGAGCCCGGCACCTTCAAGGATCGCGTGCTGCTCACGCAGGCGCCGCACCTCATGTTCGACGGCATGACCATCGCCGGCTACGCCTTGGGGCCGGGAGGGCGTGGTCTATCTGCGCGGCGAGTACGCCTATCTGTG GGAGCCTCTGCATGCGGTCCTGCGCGAGCGCTATGGGCTCGGGCTCGCCGGCGCGAACATCCTGGGACACGCGGGCTTCGACTTCGACATCCGCATCCAGCTGGGCGCCGGCGCCTATATCTGCGGCGAGGAATCCGCGCTGGTGGAATCGCTGGAAGGCAAGCGCGGCTCGCCCCGCGACCGCCCCCCCTTCCCCACCGTGCGCGGCCATCTCCAGCAGCCCACCGCCGTGGACAATGTGGAGACCTT CGCCTGCGCCGCCCGCATCCTGGAGGATGGCGTGGAGGCGTTCGCGGGCATCGGCACGCCCGAATCCGCCGGCACGAAGCTCCTCTCGGTGTCGGGCGATTGCCCGCGCCCCGGCGTGTATGAGGTGCCCTTCGGCCTCACGGTGAACGCGCTGCTCGACCTTGTCGGCGCGCCGGACGCCGCCTTCGTGCAGATGGGTGGGCCGTCCGGCCAATGCGTGGCGCCGAAGGATTACGGCCGC CGCATCGCCTTCGAGGACCTGCCCACCGGCGGCTCGGTGATGGTGTTCGGCCCGGGGGCGCGACGTGCTCGCCATGGTGCGCGAGTTCGCGGATTTCTTCGCCGGCGAATCCTGCGGCTGGTGCACGCCCTGCCGGGTGGGCACCACCTTGCTCAAGGAAGAGCTGGACAAGCTCCTCGCCAACCGCGCCACCCTCGCCGACATCCGCGCGCTGGAGACCCTGGCCACGACCGTCTCCCGCACCAGC CGCTGCGGCCTCGGCCAGACGGCGCCCAACCCCATCCTTTCCACCATGCGCAACCTGCCGGAAGCCTATGAGGCGAGGCTGAGGCCCGAAGACTTCCTGCCCTGGGCCTCGCTCGACGAGGCGCTGAAGCCCGCCATCGTCATCCAGGGCCGCGCGCCCGTGCCGGAGGAAGAGGCATGA

SEQ ID NO: 7:

Amino acid sequence of the alpha subunit of NAD+ ^- reducing hydrogenase HoxS:

MMPSEPHGAGMPPPREAAAVPTPQEVSAVVAEVVADAVASVGGARTRLMDIVQLAQQRLGHLSEETMAAIAARLAIPPVEVADMVSFYAFLNRAPKGRYHIRLSRSPISLMKGAEAVAAAFCQILGIAMGETSQDGDFTLEWTNDIGMADQEPAALVNGTVMTQLAPGDAAIIVGRLRAHHAPNALPLFPGAGVAGSGLPHARIRPSLVMPGQLLFREDHTTPGA GIKAALALTPDEVVQKVSAARLRGRGGAGFPTGLKWKLCRQSPATTRHVICNADEGEPGTFKDRVLLTQAPHLMFDGMTIAGYALGAREGVVYLRGEYAYLWEPLHAVLRERYGLGLAGANILGHAGFDFDIRIQLGAGAYICGEESALVESLEGKRGSPRDRPPFPTVRGHLQQPTAVDNVETFACAARILEDGVEAFAGIGTPESAG TKLLSVSGDCPRPGVYEVPFGLTVNALLDLVGAPDAAFVQMGGPSGQCVAPKDYGRRIAFEDLPTGGSVMVFGPGRDVLAMVREFADFFAGESCGWCTPCRVGTTLLKEELDKLLANRATLADIRALETLATTVSRTSRCGLGQTAPNPILSTMRNLPEAYEARLRPEDFLPWASLDEALKPAIVIQGRAPVPEEEA

SEQ ID NO:8:

Nucleotide sequence of the beta subunit of NAD+ ^- reducing hydrogenase HoxS:

ATGAGCCGGGGATCCCCCGATGCCGGGAAAGACCGCACCATGAGCGCCACCGACGGCACCACCGCCCCCCGCAAGATCGTCATCGATCCGGTGACCCGCGTGGAGGGCCACGGCAAGGTCACCATCCGCCTGGATGAAGCCGGCGCGGTGGAGGATGCGCGTTTCCACATCGTGGAGTTCCGCGGCTTCGAGCGGTTCATCCAGGGCCGGATGTACTGGGAAGTGCCCCTTATCATCCAGCGGC TGTGCGGCATCTGCCCGGTGAGCCACCATCTGGCGGCGGCGAAAGCCATGGACCAGGTGGCGGGCGTGGACCGCGTACCGCCCACCGCCGAGAAACTGCGCCGGCTGATGCATTATGGGCAGGTGCTGCAATCCAACGCTTTGCACATCTTCCACCTCGCCTCGCCCGACCTCCTGTTCGGCTTCGACGCGCCGGCCGAGCAGCGCAACATCATCGCCGTGCTCCAGCGTTATCCGGAGATCGGCAAATG GGCGATCTTCATCAGGAAGTTCGGCCAGGAGGTCATCAAGGCCACCGGCGGGCGCAAGATCCATCCCACCAGCGCCATTCCCGGCGGGGTCAACCAGAACCTCGCCGTGGAGGACCGCGACGCCCTGCGCGCCAAGGTGGGCGAGATCATCAGCTGGTGCATGGCGGCGCTGGACCATCACAAGGCCTATGTGGCGGAAAACCGGGCGCTGCATGACAGCTTCGCCGCCTTCCCCTCCGCCTT CATGAGCCTCGTGGGGCCGGATGGCGGCATGGACCTTTATGACGGCACCCTGCGGGTGATCGATGCCGAGGGCGCCCCCCTCATCGAAGGCGCGCCGCCCGCCTCCTACCGCGACCACCTCATCGAGGAGGTGCGGCCCTGGAGCTATCTGAAATTCCCCCATCTGCGCGCCTTCGGCCGCGACGATGGCTGGTATCGGGTCGGCCCCCTCGCCCAGGTCAATTGCGCCGCGTCCATCGACACGCCCCGCG CCGAGGCGGCCCGGCGGGACTTCATGGCCGAGGGCGGCGGCAAGCCGGTGCATGCCACCCTCGCTTATCACTGGGCGCGGCTCATCGTGCTGGTCCATTGCGCGGAGAAGATCGAACAGCTGCTGTTCGACGACGACCTGCAAGGCTGCGATCTGCGTGCGGAGGGCACCCGGCGCGGGGAAGGCGTCGCCTGGATCGAGGCGCCGCGCGGCACCCTCATCCACCATTACGAGGTGGACGAGAACGAC CAGGTGCGCCGCGCCAACCTCATCGTCTCCACCACCCACAATAACGAGGCCATGAACCGCGCCGTGCGGCAGGTGGCGAAGACGGACCTTTCCGGTCGCGAGATCACCGAAGGGCTGCTGAACCATATCGAGGTGGCCATCCGCGCCTTCGACCCCTGCCTGTCCTGCGCCACCCATGCGCTGGGCCAGATGCCGCTGATCGTGACGCTTGAAGATGCCTCCGGCGCAGAGATCGCCCGCGGAGT GAAGGAATGA

SEQ ID NO:9:

Amino acid sequence of the beta subunit of NAD+ ^- reducing hydrogenase HoxS:

MSRGSPDAGKDRTMSATDGTTAPRKIVIDPVTRVEGHGKVTIRLDEAGAVEDARFHIVEFRGFERFIQGRMYWEVPLIIQRLCGICPVSHHLAAAKAMDQVAGVDRVPPTAEKLRRLMHYGQVLQSNALHIFHLASPDLLFGFDAPAEQRNIIAVLQRYPEIGKWAIFIRKFGQEVIKATGGRKIHPTSAIPGGVNQNLAVEDRD ALRAKVGEIISWCMAALDHHKAYVAENRALHDSFAAFPSAFMSLVGPDGGMDLYDGTLRVIDAEGAPLIEGAPPASYRDHLIEEVRPWSYLKFPHLRAFGRDDGWYRVGPLAQVNCAASIDTPRAEAARRDFMAEGGGKPVHATLAYHWARLIVLVHCAEKIEQLLFDDDLQGCDLRAEGTRRGEGVAWIEAPRGTLIHHYEVDENDQ VRRANLIVSTTHNNEAMNRAVRQVAKTDLSGREITEGLLNHIEVAIRAFDPCLSCATHALGQMPLIVTLEDASGAEIARGVKE

SEQ ID NO: 10:

Nucleotide sequence of the gamma subunit of NAD+ ^- reducing hydrogenase HoxS:

ATGAGCGAGACCCCCTTCACCTTTACCGTGGACGGCATCGCGGTCCCGGCCACCCCCGGCCAGAGCGTCATCGAGGCGTGCGATGCGGCGGGCATCTATATCCCGCGCCTGTGCCACCACCCGGACCTGCCGCCGGCGGGCCATTGCCGGGTGTGCACCTGCATCATCGACGGGCGGCCGGCCAGCGCCTGCACCATGCCCGCCGCCAGGGGCATGGTGGTGGAGAACGAGACGCCCGCTTTGC TGGCGGAGCGGCGCACGCTGATCGAGATGCTGTTCGCGGAAGGCAACCATTTCTGCCAGTTCTGCGAGGCGAGCGGCGATTGCGAATTGCAGGCGCTGGGCTACCTGTTCGGCATGGTGGCCCCGCCCTTCCCCCATCTGTGGCCGAAGCGGCCGGTGGATGCCAGCCATCCGGATATCTATATCGACCACAATCGCTGCATCCTGTGCTCGCGCTGCGTGCGCGCCTCGCGCCACCCTGGACGG CAAGTCCGTGTTCGGCTTCGAGGGGCGCGGCATCGGATGCATCTGGCGGTGACCGGCGGGCACCTGGACGACAGCGCCATCGCCGCCGCCGACAGGGCGGTTGAGATGTGCCCGGTGGGCTGCATCGTCCTCAAGCGCACCGGCTACCGCACGCCCTATGGCCGGCGGCGCTACGACGCCGCGCCCATCGGCTCCGACATCACCGCCCGGCGCGGCGGCGCGAAGGACTGA

SEQ ID NO: 11:

Amino acid sequence of the gamma subunit of NAD+ ^- reducing hydrogenase HoxS:

MSETPFTFTVDGIAVPATPGQSVIEACDAAGIYIPRLCHHPDLPPAGHCRVCTCIIDGRPASACTMPAARGMVVENETPALLAERRTLIEMLFAEGNHFCQFCEASGDCELQALGYLFGMVAPPFPHLWPKRPVDASHPDIYIDHNRCILCSRCVRASRTLDGKSVFGFEGRGIEMHLAVTGGHLDDSAIAAADRAVEMCPVGCIVLKRTGYRTPY GRRRYDAAPIGSDITARRGGAKD

SEQ ID NO: 12:

Nucleotide sequence of the delta subunit of NAD+ ^- reducing hydrogenase HoxS:

ATGGCCAAGCCCAAACTCGCCACCTGCGCGCTGGCCGGCTGCTTCGGCTGCCACATGTCCTTCCTGGACATGGACGAGCGCATCGTCGAGCTCATCGACCTGGTGGACCTCGACGTCTCGCCCCTCGACGACAAGAAAAACTTCACCGGCATGGTGGAAATCGGCCTGGTGGAAGGCGGCTGCGCCGACGAGCGCCATGTGAAGGTGCTGCGCGAGTTCCGCGAGAAATCCCGCATCCTGGTG GCGGTGGGCGCCTGCGCCATCACCGGCGGCATCCCGGCATTGCGCAACCTCGCCGGCCTCGACGAATGCCTGAGGGAAGCCTACCTCACCGGCCCCACGGTGGAAGGCGGCGGGCTCATTCCCAACGACCCGGAGCTGCCGCTGCTGCTGGACAAGGTCTATCCGGTGCAGGACTTCGTGAAGATCGACCATTTCCTGCCCGGCTGCCCGCCCTCGGCCGACGCCATCTGGGCGGCTCTGAAGGCGC TGCTGACCGGCACCGAGCCGCATCTGCCCTACCCGCTTTTCAAGTACGAATGA

SEQ ID NO: 13:

Amino acid sequence of the delta subunit of NAD+ ^- reducing hydrogenase HoxS:

MAKPKLATCALAGCFGCHMSFLDMDERIVELIDLVDLDVSPLDDKKNFTGMVEIGLVEGGCADERHVKVLREFREKSRILVAVGACAITGGIPALRNLAGLDECLREAYLTGPTVEGGGLIPNDPELPLLLDKVYPVQDFVKIDHFLPGCPPSADAIWAALKALLTGTEPHLPYPLFKYE

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding a large subunit of NiFeSe hydrogenase having the sequence set forth in SEQ ID NO:15, or a sequence having greater than 84% sequence identity, such as greater than 90% sequence identity , for example, more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:15.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding a small subunit of NiFeSe hydrogenase having the sequence specified in SEQ ID NO:17, or a sequence having more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:17.

SEQ ID NO: 14:

Nucleotide sequence of the large subunit of periplasmic hydrogenase [NiFeSe]:

TCCAGACCCGGGCAACATTGCTCCATGTGCTGGGCACCCTGGCCGGCCGCTGGCCCCATACCCTCGCGCTCCAGCCCGGCGGGGTGACCCGAAGCGCCGACCAGCACGACCGCATGCGCCTGCTCGCGACGCTGAAGGCGGTGCGGGCGGCGCTGGAAGAGACCTTGTTCGGCGCGCCTTTGGAAGAGGTGGCGGCCCTGGACGGCGCCGCCGCCGTGGAGGCCTGGCGCGCCAACGGCCCGGA AGGGGATTTCCGCCTGTTCCTGGAGATCGCCGCCGACCTGGAGCTGGACCGGCTCGGCCGCGCGCACGACCGCTTTCTCTCCTTCGGCGCCTACGCCCAGGACGAGGGGCGCCTTTATGGCGCCGGCACCTTCGAGGCCGGGACGGCGGGAGGGCTCGATCCCAACGCCATCACCGAGGACCACGCCTTCGCCCGCATGGAGGACCGCGCGCGCCCCATGCGCCCTTTGACGGCTCCACCTTCCCC GATGCCGACGACACCGAGGGCTACACCTGGTGCAAGGCGCCGCGCCTTGCCGGCCTGCCCTTCGAGACCGGCGCCTTCGCCCGGCAGGTGGTGGCGGGCCATCCGCTCGCCCGGGACCTCGTGACGCGGGAAGGCGGCACTGTGCGCAGCCGCGTGGTCGGCCGGCTGCTGGAAACCGCGCGCCACCCTGATCGCCATGGAGGGCTGGGTGAAGGAACTGCGGCCCGAAGGGCCCTGGTGCG CCCAGGGCCACCTGCCCCAGGAAGGCCGCGCCTTCGGCCTCACCGAGGCGGCGCGGGGCGCTCGGCCACTGGATGGTGGTGGAGAAGGGCCGCATTGCCCGCTACCAGATCATCGCCCCCACCACCTGGAACTTCTCCCCCCGCGACGGCGCGGGCCTGCCCGGCCCGCTGGAGACGGCCCTGGTGGGCGCGCCCGTGCGGCAGGGAGAGACGACGCCCGTGAGCGTGCAGCACATCGTGC GCTCCTTCGACCCGTGCATGGTCTGCACTGTGCATTGA

SEQ ID NO: 15:

Amino acid sequence of the large subunit of periplasmic hydrogenase [NiFeSe]:

MSAETRRLVVGPFNRVEGDLEVRLDVQDGRVQQAFVSSPLFRGFERILEGRDPRDALVIAPRICGICSVSQSHAAALALAGLQGIAPTHDGRIATNLIVAAENVADHLTHFHVFFMPDFARAVYEDRPWFAQAARRFKANQGVSVRRALQTRATLLHVLGTLAGRWPHTLALQPGGVTRSADQHDRMRLLATLKAVRAALEETLFGAPL EEVAALDGAAAVEAWRANGPEGDFRLFLEIAADLELDRLGRAHDRFLSFGAYAQDEGRLYGAGTFEAGTAGGLDPNAITEDHAFARMEDRAAPHAPFDGSTFPDADDTEGYTWCKAPRLAGLPFETGAFARQVVAGHPLARDLVTREGGTVRSRVVGRLLETARTLIAMEGWVKELRPEGPWCAQGHLPQEGRAFGLTEAARGALGHWMVVEKGRIARYQIIAP TTWNFSPRDGAGLPGPLETALVGAPVRQGETTPVSVQHIVRSFDPCMVCTVH

SEQ ID NO: 16:

Nucleotide sequence of the small subunit of periplasmic hydrogenase [NiFeSe]

ACGGGGGAGGAAGCCCGCGCCATCTTCGACGCCATCCTTGCCGGCGTTATCGTCCTCGACGCCCTGTGCGTGGAAGGCGCGCTGCTGCGCGGGCCGAACGGCACCGGGCGCTTCCATGTGCTGGCGGGCACGGACACCCCCACCATCGACTGGGCGCGGCAGCTCGCCGGCATGGCGCGCCACGTGGTGGCGGTGGGCACCTGCGCCGCCTATGGGGGCGTGACGGCGGCGGGCATCAACCC CACCGATGCCTGCGGCCTCCAGTTCGACGGACGCCGGAAGGGTGGGGCGCTGGGGGCGGACTTCCGCTCCCGCTCGGGGCTTCCGGTCATCAATGTGGCCGGCTGCCCCACCCATCCCAACTGGGTGACGGAAACCCTGATGCTGCTCGCCTGCGGCCTGCTGGGCGAGGCCGACCTCGACGTCTATGGCCGCCCGCTTCTATGCGGACCTGCTGGTGCATCACGGCTGCCCGCGCAACGAATACT ATGAATACAAGGCGAGCGCCGAGAAGATGAGCGACCTCGGCTGCATGATGGAGCATCTGGGCTGCCTCGGCACCCAGGCCCACGCCGACTGCAACACGCGCCTTTGGAATGGCGAGGGCTCGTGCACCCGCGGCGGCTATGCCTGCATCAACTGCACGGCGCCGGAATTCGAGGAGCCGGGCCACGCCTTCCTGGAGACGCCCAAGATCGGCGGCATCCCCATCGGCCTGCCCACCGACATGCCCAAG GCCTGGTTCATCGCCTTGTCCTCCCTCGCCAAGGCGGCGACGCCGGAGCGGCTGCGCAAGAACGCGGTGTCCGACCATGTGGTCACGCCGCCCGCCGTCAAGGACATCAAGCGGCGATGA

SEQ ID NO: 17:

Amino acid sequence of the small subunit of periplasmic hydrogenase [NiFeSe]

MSTPFSVLWLQSGGCGGCTMSLLCAEAPDLATTLDAAGIGFLWHPALSEETGEEARAIFDAILAGVIVLDALCVEGALLRGPNGTGRFHVLAGTDTPTIDWARQLAGMARHVVAVGTCAAYGGVTAAGINPTDACGLQFDGRRKGGALGADFRSRSGLPVINVAGCPTHPNWVTETLMLLACGLLGEADLDVYGRPRFYADRNLLVHHGCPEY YEYKASAEKMSDLGCMMEHLGCLGTQAHADCNTRLWNGEGSCTRGGYACINCTAPEFEEPGHAFLETPKIGGIPIGLPTDMPKAWFIALSSLAKAATPERLRKNAVSDHVVTPPAVKDIKRR

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the gamma chain of ATP synthase atpG_1 having the sequence specified in SEQ ID NO:19, or a sequence having more than 70% identity, for example more than 80% identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:19.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the alpha subunit of ATP synthase atpA_1 having the sequence specified in SEQ ID NO:21, or a sequence having greater than 78% identity, for example greater than 80% identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:21.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the b subunit of ATP synthase atpF_1 having the sequence specified in SEQ ID NO:23, or a sequence having more than 62% identity, for example more than 70% identity , for example, more than 80% identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:23.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the c subunit of the sodium ion-specific ATP synthase atpE_1 having the sequence specified in SEQ ID NO:25, or a sequence having 90% identity, for example more 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:25.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the ATP synthase subunit a atpB_1 having the sequence set forth in SEQ ID NO:27, or a sequence having greater than 80% identity, such as greater than 90% identity , for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:27.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the epsilon chain of the ATP synthase atpC_1 having the sequence set forth in SEQ ID NO: 29, or a sequence having greater than 71% identity, such as greater than 80% identity. identity, for example more than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:29.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the beta subunit of ATP synthase atpD_1 having the sequence specified in SEQ ID NO:31, or a sequence having greater than 84% identity, for example greater than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:31.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the beta subunit of ATP synthase atpD_2 having the sequence specified in SEQ ID NO:33, or a sequence having greater than 97% identity, for example greater than 98% , for example, more than 99% sequence identity with the sequence presented in SEQ ID NO:33.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the gamma chain of ATP synthase atpG_2 having the sequence specified in SEQ ID NO:35, or a sequence having greater than 86% identity, for example greater than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:35.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding an ATP synthase alpha subunit atpA_2 having the sequence set forth in SEQ ID NO: 37, or a sequence having greater than 98%, for example greater than 99%, identity to sequence with the sequence specified in SEQ ID NO:37.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the delta subunit of ATP synthase atpH having the sequence specified in SEQ ID NO:39, or a sequence having greater than 85% identity, for example greater than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:39.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding ATP synthase subunit b atpF_2 having the sequence set forth in SEQ ID NO:41, or a sequence having greater than 87% identity, such as greater than 90% identity , for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:41.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the b' subunit of ATP synthase atpG_3 having the sequence specified in SEQ ID NO:43, or a sequence having greater than 81% identity, for example greater than 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:43.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the c subunit of ATP synthase atpE_2 having the sequence specified in SEQ ID NO:45, or a sequence having greater than 98%, for example greater than 99%, identity to sequence with the sequence presented in SEQ ID NO:45.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the ATP synthase subunit a atpB_2 having the sequence set forth in SEQ ID NO:47, or a sequence having greater than 92% identity, such as greater than 95% identity , for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:47.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding ATP synthase protein I atpI having the sequence set forth in SEQ ID NO: 49, or a sequence having greater than 60% identity, such as greater than 70% identity , for example, more than 80% identity, for example more than 90% identity, for example, more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:49.

SEQ ID NO: 18:

Nucleotide sequence of the gamma chain of ATP synthase atpG_1

GTGACCGAGCGCCTGTCCGACGTCAACGCCCGCATCGCCTCGGTGCGGCAGCTCTCATCGGTCATCACGGCCATGCGGGGCATTGCGGCGGCGCGGGCGCGGGAGGCGCGGGGTCGGCTCGACGGCATCCGCGCCTATGCGCAGACCATCGCCGAGGCCATCGGCCATGTGCTCGCCGTGCTGCCCGAGGAGGCCCGCGCCCGGTCCTCCGGGCACCGGCATCGGGGCCATGCGGTCATCGCCCTG TGCGCGGAGCAGGGCTTTGCCGGCGTCTTCAACGAGCGGGTGCTGGACGAGGCCGCCCGGCTGCTGACCGGCGGGGCGGGGCCGGCCGAGCTGCTGCTGGTGGGCGACCGGGGCCTGATGGTGGCCCGCGAGCGGGGGCTCGATGTCTCCTGGTCGGTGCCCATGGTGGCCCATGCGGGCCAGGCCTCGGCGCTGGCGGACCGCATCAGCGAGGAGCTCTACCGGCGGATCGATGCGGGACGGG TGACGCGGGTGTCGGTGGTGCACGCCGAGCCCGCCGCGTCCGCCGCCATCGAGACGGTGGTGAAAGTGCTGGTGCCGTTCGACTTCGCCCGCTTCCCCCTGGCGCGGGTGGCATCCGCCCCGCTCATGACCATGCCGCCGCCGCGGCTGCTGGCCCAGCTGTCGGAGGAATATGTGTTCGCCGAGCTGTGCGAGGCGCTCACCTTGTCCTTCGCGGCGGAGAACGAGGCCCGCATGCGGGCCATGATC GCCGCCCGCGCCAATGTGGCCGATACCCTGGAGGGCCTCGTCGGCCGCGCCCGGCAGATGCGCCAGGAGGAGATCACCAACGAGATCATCGAGCTGGAAGGCGGCGCCGGCAGCGCCCGGCATGCGGATTGA

SEQ ID NO: 19:

Amino acid sequence of the gamma chain of ATP synthase atpG_1

MTERLSDVNARIASVRQLSSVITAMRGIAAARAREARGRLDGIRAYAQTIAEAIGHVLAVLPEARARSSGHRHRGHAVIALCAEQGFAGVFNERVLDEAARLLTGGAGPAELLLVGDRGLMVARERGLDVSWSVPMVAHAGQASALADRISEELYRRIDAGRVTRVSVVHAEPAASAAIETVVKVLVPFDFARFPLARVASAPLMTMPPPRLLAQLS EEYVFAELCEALTLSFAAENEARMRAMIAARANVADTLEGLVGRARQMRQEEITNEIIELEGGAGSARHAD

SEQ ID NO: 20:

Nucleotide sequence of the alpha subunit of ATP synthase atpA_1

ATGAGCACGGGCGCGCAAGCGAGCGAGGATTGGCTCACCCGGAGCCGGGCGGCCCTGGCCGGGACGCGCCTTTCCCAGCAATCCCAATCGGTGGGCCGGGTGGAGGAGATGGCCGACGGCATCGCCCGCGTCTCCGGCCTGCCGGATGTGCGGCTCGACGAGCTTCTCACCTTCGAGGGCGGCCAGACCGGCTATGCCCTCACCCTCGATCGCACCGAGATCGCCGTGGTGCTGCTGGATG ACGCCTCCGGCGTGGAGGCGGGCGCCCGGGTGTTCGGCACCGGCGAGGTGGTGAAGGTGCCGGTGGGGCCGGGGCTGCTGGGCCGCATCGTCGACCCCCTCGGCCGGCCCATGGACCGCTCCGAGCCGGTGGTGGCGCAGGCGCACCATCCCATCGAGCGGCCGGCGCCGGCCATCATCGCCCGCGACCTGGTCTCGCAGCCGGTTCAGACCGGCACGCTGGTGGTGGATGCGCTGTTCCTCCCTC GGCCGGGGCCAGCGCGAGCTCATCATCGGCGACCGGGCTACCGGCAAGACCGCCATCGCGGTGGACACCATCATCAGCCAGAAGCATTCGGACATCGTGTGCATCTACGTGGCGGTGGGCCAGCGCGCCGCCGCCGTGGAGCGGGTGGTGGAGGCGGTGCGCGCCCACGGGGCGATCGAGCGCTGCATCTTCGTGGTCGCCTCGGCCGCCGCCTCGCCAGGGCTGCAATGGATCGCGCCGTTCG CCGGCATGACCATGGCGGAATATTTCCGCGACAACGGCCAGCATGCGCTCATCATCATCGATGATCTCACCAAGCATGCGGCCACCCATCGCGAGCTGGCGCTGCTCACCCACGAGCCGCCGGGCCGCGAGGCCTATCCCGGCGACATCTTCTATGTGCACGCCCGCCTTCTGGAGCGGGCCGCCAAGCTCTCCGCCGAGCTGGGCGGTGGCTCGCTCACGGCCCTGCCCATCGCGGAGACGGACGCGGGAAA CCTCTCCGCCTATATCCCCACCAACCTCATCTCCATCACCGATGGGCAGATCGTGCTGGATTCGCGGCTGTTCGCGGCCAACCAGCGCCCGGCGGTGGATGTGGGCCTCTCCGTGAGCCGGGTGGGCGGCAAGGCGCAGCATCCCGCGCTTCGGGCCGTGTCCGGGCGCATCCGGCTCGATTATTCCCAGTTCCTGGAGCTGGAAATGTTCACCCGCTTCGGCGGCATCACCGATACCCGCG TGAAGGCGCAGATCACCCGGGGCGAGCGCATCCGCGCGCTGCTCACCCAGCCGCGCTTTTCCACCCTGCGCCTTCAGGACGAGGTGGCGCTGCTGGCCGCGCTGGCGGAGGGGGTGTTCGACACTTTGGCCCCGGGGCTGATGGGCGCCGTGCGTGCCCGCATTCCGGCCCAGCTGGATGCGCAGGTGAAGGACGTGGCCTCGGCCCTCGCCGAGGGCAAGGTGCTGGAGGAGGGCTTGCACGC CCGTCTCGTGGCGGCCGTGCGGGCCGTCGCGGCGGACGTGGCCGCGACCGCGAAGGCCGGGCCGTGA

SEQ ID NO: 21:

Amino acid sequence of the alpha subunit of ATP synthase atpA_1

MSTGAQASEDWLTRSRAALAGTRLSQQSQSVGRVEEMADGIARVSGLPDVRLDELLTFEGGQTGYALTLDRTEIAVVLLDDASGVEAGARVFGTGEVVKVPVGPGLLGRIVDPLGRPMDRSEPVVAQAHHPIERPAPAIIARDLVSQPVQTGTLVVDALFSLGRGQRELIIGDRATGKTAIAVDTIISQKHSDIVCIYVAVGQ RAAAVERVVEAVRAHGAIERCIFVVASAAASPGLQWIAPFAGMTMAEYFRDNGQHALIIIDDLTKHAATHRELALLTHEPPGREAYPGDIFYVHARLLERAAKLSAELGGGSLTALPIAETDAGNLSAYIPTNLISITDGQIVLDSRLFAANQRPAVDVGLSVSRVGGKAQHPALRAVSGRIRLDYSQFLELEMFTRFGGITDTRVKAQITRGERIR ALLTQPRFSTLRLQDEVALLAALAEGVFDTLAPGLMGAVRARIPAQLDAQVKDVASALAEGKVLEEGLHARLVAAVRAVAADVAATAKAGP

SEQ ID NO: 22:

Nucleotide sequence of subunit b of ATP synthase atpF_1

ATGCAGATCGACTGGTGGACGCTGGGCCTGCAGACGGTCAACGTCCTCGTTCTCATCTGGCTCCTGAGCCGCTTCCTGTTCAAGCCGGTGGCGCCAGGTCATCGCGCAGCGCCGTGCCGAGATCGAGAAGCTGGTGGAGGATGCGCGCGCCGCCAAGGCCGCCGCCGAGGCCGAGCGGGACACGGCGAAGGCGGAGGAGGCGCGCCTTGCCGCCGAGCGCGGCGCCCGCATGGCGGCGGTCGCCAAG GAGGCGGAGGCGCAGAAGGCGGCATTGCTGGCCGCCGCCAAGACCGAGGCCGAGGCCCTGCACGCGGCCGCGGAAGCGGCCATCGTCCGGGCGCGGGCGAGCGAGGAGGAAGCCGCCGCCGACCGCGCCAGCCGCCTTGCCGTGGACATCGCCGCCAAGCTGCTGGACCGGCTGCCCGACGACGCCCGGGTCGCGGGCTTCATCGATGGCCTCGCCGAGGGGCTTGAAGCCCTGCCCGAGGCGAGCC GGGCGGTGATCGGCGTCGACGGCGCGCAGTGCGCGTGACGGCCGCGCGCCCCTTATGCCGGCGGAGGAGGAGGCCTGCCGCACGCGGCTCTCCCAGGCGCTGGGCCGTCCGGTGACGCTGGCCGTGACCATCGACCCCGCCCTCATCGCCGGCCTGGAGATGGAGACGCCCCACGCGGTGGTGCGCAATTCCTTCAAGGCCGATCTCGACCGCGTCACCGCGGCGCTCACCCATCATGGGA CCTGA

SEQ ID NO: 23:

Amino acid sequence of ATP synthase subunit b atpF_1

MQIDWWTLGLQTVNVLVLIWLLSRFLFKPVAQVIAQRRAEIEKLVEDARAAKAAAEAERDTAKAEEARLAAERGARMAAVAKEAEAQKAALLAAAKTEAEALHAAAEAAIVRARASEEEAAADRASRLAVDIAAKLLDRLPDDARVAGFIDGLAEGLEALPEASRAVIGVDGAPVRVTAARALMPAEEEACRTRLSQALGRPVTLAVTIDPALIAGLEM ETPHAVVRNSFKADLDRVTAALTHHGT

SEQ ID NO: 24:

Nucleotide sequence of subunit c of the sodium ion-specific ATP synthase atpE_1

ATGACTGTCGAGATGGTCAGCATCTTCGCGGCGGCGCTCGCCGTCTCCTTCGGCGCCATCGGGCCGGCCCTGGGCGAGGGCCGGGCGGTGGCCGCGGCCATGGACGCCATCGCCCGCCAGCCGGAGGCGGCCGGAACCTTGTCGCGCACGCTCTTCGTCGGCCTCGCCATGATCGAGACCATGGCGATCTACTGCCTGGTGATCGCGCTCCTGGTGCTCTTCGCCAATCCGTTCGTGAAGT GA

SEQ ID NO: 25:

Amino acid sequence of subunit c of the sodium ion-specific ATP synthase atpE_1

MTVEMVSIFAAALAVSFGAIGPALGEGRAVAAAMDAIARQPEAAGTLSRTLFVGLAMIETMAIYCLVIALLVLFANPFVK

SEQ ID NO: 26:

Nucleotide sequence of subunit a of ATP synthase atpB_1

ATGGGCTCGCCGCTGATCCTCGAACCCCTGTTCCATATCGGGCCCGTGCCCATCACCGCGCCGGTGGTGGTCACCTGGCTCATCATGGCCGCCTTCATTGGGCTGGCGCGGCTCATCACCCGGAAGCTTTCCACCGATCCCACCCGGACCCAGGCGGCGGTGGAAACGGTGCTGACCGCCATCGATTCCCAGATCGCCGACACCATGCAGGCCGATCCCGCGCCTTATCGCGCTCATCGGCAC CATCTTCCTTTATGTGCTGGTGGCCAACTGGTCCTCGCTCATCCCGGGCATCGAGCCGCCCACGGCGCATATCGAGACCGATGCGGCGCTCGCTTTCATCGTGTTCGCCGCCACCATCGGGTTCGGGTTGAAGACAAGGGGTGTGAAGGGCTATCTCGCCACCTTCGCCGAACCCTCCTGGGTGATGATCCCGCTCAATGTGGTGGAGCAGATCACCCGGACCTTCTCGCTCATCGTGCGCCTGTTCGGC AACATCATGAGCGGGGTGTTCGTGGTCGGCATCCTGTCCCTCGCCGGGCTGCTGGTGCCCATCCCCCTCATGGCGCTCGATCTCCTGACCGGCGCCGTGCAGGCCTACATCTTCGCGGTGCTGGCCTGCGTGTTCATCGGCGCGGCCATTGGCGAGGCGCCGGCAAAGCCCCAATCGAAGGAGCCAGGGAAAACATCATGA

SEQ ID NO: 27:

Amino acid sequence of subunit a of ATP synthase atpB_1

MGSPLILEPLFHIGPVPITAPVVVTWLIMAAFIGLARLITRKLSTDPTRTQAAVETVLTAIDSQIADTMQADPAPYRALIGTIFLYVLVANWSSLIPGIEPPTAHIETDAALAFIVFAATIGFGLKTRGVKGYLATFAEPSWVMIPLNVVEQITRTFSLIVRLFGNIMSGVFVVGIILSLAGLLVPIPLMALDLLTGAVQAYIFAVLACV FIGAAIGEAPAKPQSKEPGKTS

SEQ ID NO: 28:

Nucleotide sequence of the epsilon chain of ATP synthase atpC_1

GTGAGCGCGCCGCTGCACCTCACCATCACCACGCCGGCCGCCGTTCTGGTGGACCGTGCCGACATCGTGGCCCTGCGTGCCGAGGACGAGAGCGGCAGCTTCGGCATCCTGCCCGGCCATGCGGATTTCCTGACCGTTCTGGAGGCCTGCGTGGTGCGCTTCAAGGATGGGGCCGACGGCGTGCATTATTGTGCTCTCAGTGGTGGCGTGCTGTCGGTCGAGGAGGGCCGGCGCATC GCCATCGCCTGCCGTCAGGGCACGTGAGCGACGACCTGGTCGCCCTGGAAGGGGCGGTGGACGCCATGCGTTCGGCGGAGAGCGATGCCGACAAGCGGGCCCGGGTGGAGCAGATGCGCCTTCATGCCCACGCCGTGCGCCAGCTCTGCACTATCTGCGGCCCGGCCGGGCCGGCGGCGTGGCGCCGGCCGCCGCGCCGGAGGAGGGGCCGTCATGA

SEQ ID NO: 29:

Amino acid sequence of the epsilon chain of ATP synthase atpC_1

MSAPLHLTITTPAAVLVDRADIVALRAEDESGSFGILPGHADFLTVLEACVVRFKDGADGVHYCALSGGVLSVEEGRRIAIACRQGTVSDDLVALEGAVDAMRSAESDADKRARVEQMRLHAHAVRQLLHYLRPGRAGGVAPAAAPEEGPS

SEQ ID NO: 30:

Nucleotide sequence of the beta subunit of ATP synthase atpD_1

ATGGCAGCGGCAGATGAGGAGGCGCAATCGGCCGCCGGCCCCGCCTCGGGCCGGGTGGTGGCCGTGCGCGGCGCGGTGATCGACATCGCCTTTGCCCAGCCTCCGCTGCCGCCGCTGGACGACGCCCTTCTCATCACCGACGGCCCGGGGCGGCACGGTGCTGGTGGAGGTGCAGAGCCATATGGATCGGCACACGGTGCGCGCCATCGCCCTTCAGGCCACCACCGGCCTCAGCCGGGGGCT GGAGGCGGCGCGGGTGGGCGGGCCGGTGAAGGTGCCGGTGGGAGACCATGTGCTCGGCCGCCTCCTGGATGTCACCGGCGCCATCGGCGACAAGGGCGGGCCGCTGCCGGCCGACGTGCCCACGCGGCCGATCCACCACGCGCCGCCATCCTTCGCCGCGCAGGGCGGCACGTCCGATCTGTTTCGCACCGGCATCAAGGTCATCGACCTCCTGGCGCCCCTCGCCCAGGGCGGCAAGGCGGC CATGTTCGGCGGGGCCGGCGTGGGCAAGACCGTGCTGGTGATGGAGCTGATCCACGCCATGGTGGCGAGCTACAAGGGCATCTCGGTGTTTGCCGGCGTGGGGGAGCGCTCCCGCGAGGGCCACGAGATGCTGCTGGACATGACCGATTCCGGCGTGCTCGACCGCACCGTTCTGGTCTATGGCCAGATGAACGAGCCCCCCGGGGCCCGCTGGCGGGTGCCCATGACGGCGCTGACCATCGCCGAATA TTTCCGCGACGAGAAGCACCAGAACGTCCTGCTGCTGATGGACAACATCTTCCGCTTCGTCCAGGCGGGGGCGGAGGTCTCCGGCCTTTTGGGCCGTCCGCCCTCCCGGGTGGGATACCAGCCGACGCTGGCGAGCGAGGTGGCGGCGCTCCAGGAACGCATCACCTCCGTGGGCGAGGCCTCGGTGACCGCCATCGAGGCGGTCTACGTGCCGGCGGATGACTTCACCGATCCCGCCGTGA CCACCATCGCCGCCCACGTGGATTCCATGGTGGTGCTCTCCCGCGCCATGGCGGCGGAGGGCATGTATCCGGCGGTGGACCCCATCTCCTCCTCGTCGGTGCTGCTCGACCCGCTCATCGTGGGGGACGAGCATGCGCGCGTCGCCAACGAGGTGCGCCGGACCATCGAGCATTATCGCGAGCTTCAGGATGTGATCTCGCTGCTGGGCATGGAGGAATTGGGCACCGAGGATCGCCGCATCGTGGA GCGGGCGCGCCGGCTCCAGCGCTTCCTCACCCAGCCCTTCACGGTCACCGAGGCCTTCACCGGCGTGCCCGGCCGCTCGGTGGCCATCGCCGACACCATCGCCGGCTGCAGGATGATCCTGTCCGGCGCCTGCGACGACTGGCAGGAAAGCGCCCTCTACATGGTGGGCACCATCGACGAGGCCCGCCAGAAGGAGGAGGCCGCTCGCGCCAAGGCGGGGCAGGGCGCCCCGGCCGGGACGGCAGCCG AGACGGCGGAGGCCGCCCCGTGA

SEQ ID NO: 31:

Amino acid sequence of the beta subunit of ATP synthase atpD_1

MAAADEEAQSAAGPASGRVVAVRGAVIDIAFAQPPLPPLDDALLITDGRGGTVLVEVQSHMDRHTVRAIALQATTGLSRGLEAARVGGPVKVPVGDHVLGRLLDVTGAIGDKGGPLPADVPTRPIHHAPPSFAAQGGTSDLFRTGIKVIDLLAPLAQGGKAAMFGGAGVGKTVLVMELIHAMVASYKGISVFAGVGERSREGHEMLLDMTD SGVLDRTVLVYGQMNEPPGARWRVPMTALTIAEYFRDEKHQNVLLLMDNIFRFVQAGAEVSGLLGRPPSRVGYQPTLASEVAALQERITSVGEASVTAIEAVYVPADDFTDPAVTTIAAHVDSMVVLSRAMAAEGMYPAVDPISSSSVLLDPLIVGDEHARVANEVRRTIEHYRELQDVISLLGMEELGTEDRRIVERARRLQRFLTQPFTVTE AFTGVPGRSVAIADTIAGCRMILSGACDDWQESALYMVGTIDEARQKEEAARAKAGQGAPAGTAAETAEAAP

SEQ ID NO: 32:

Nucleotide sequence of the beta subunit of ATP synthase atpD_2

ATGGCGAACAAGGTCGGACGCATCACCCAGATCATCGGCGCCGTCGTCGACGTGCAGTTCGACGGGCATCTGCCGGCGATTCTCAACGCGATCGAGACCACCAACCAGGGCAACCGGCTGGTGCTCGAAGTGGCTCAGCATCTCGGCGAGAACACCGTGCGCTGCATCGCCATGGATGCCACTGAAGGCCTGGTGCGTGGCCAGGAGGTGGCCGACACCGATGCGCCCATCCAGGTGCC CGTGGGCGCCGCCACCCTCGGCCGCATCATGAACGTGATCGGCGAGCCGGTGGACGAGCTGGGCCCCATCGAGGGCGAAGCGCTGCGCGGCATCCATCAGCCGGCCCCCTCCTATGCGGAGCAGGCCACGGAAGCTGAGATCCTCGTCACCGGCATCAAGGTGGTGGATCTGCTGGCGCCTATCCAAGGGCGGCAAGGTGGGCCTGTTCGGCGGCGCCGGCGTGGGCAAGACCGTGCTCATCATCAT GGAGCTGATCAACAACGTGGCCAAGGCGCCACGGCGGCTATTCCGTGTTCGCCGGCGTGGGTGAGCGCACCCGCGAGGGCAACGACCTCTACCACGAGATGATCGAGTCCAACGTGAACAAGGACCCGCACGAGAACAATGGCTCGGCGGCCGGTTCCAAGTGCGCCCTGGTCTATGGCCAGATGAACGAGCCGCCCGGCGCCCGCGCCCGCGTGGCCCTCACCGGCCTCACCGTCGCCGAG CATTTCCGCGACCAGGGCCAGGACGTGCTGTTCTTCGTGGACAACATCTTCCGCTTCACCCAGGCGGGCTCCGAGGTGTCGGCGCTTCTCGGCCGCATCCCCTCGGCGGTGGGCTACCAGCCGACGCTGGCCACCGACATGGGCCAGCTGCAGGAGCGCATCACCACCACCACCAAGGGCTCCATCACCTCGGTGCAGGCCATCTACGTGCCGGCGGACGATCTGACCGATCCGGCGCCGGCC GCCTCCTTCGCCCATCTGGACGCCACCACGGTGCTGTCGCGCTCCATCGCGGAGAAGGGCATCTACCCGGCGGTGGATCCGCTGGACTCCACCTCGCGCATGCTGTCTCCCGCCATCCTCGGCGACGAGCACTACAACACCGCGCGCCAGGTGCAGCAGACCCTGCAGCGCTACAAGGCGCTCCAGGACATCATCGCCATCCTGGGCATGGACGAACTCTCCGAAGAGGACAAGCTCACCGTGGCCCGCGCCC GCAAGATCGAGCGCTTCCTCTCCCAGCCCTTCCACGTGGCCGAGGTGTTCACCGGTTCGCCCGGCAAGCTGGTCGACCTCGCCGACACCATCAAGGGCTTCAAGGGCCTGGTGGACGGCAAGTACGACTACCTGCCCGAGCAGGCCTTCTACATGGTGGGCACCATCGAAGAAGCCATCGAGAAGGGCAAGAAGCTGGCGGCCGAGGCGGCCTGA

SEQ ID NO: 33:

Amino acid sequence of the beta subunit of ATP synthase atpD_2

MANKVGRITQIIGAVVDVQFDGHLPAILNAIETTNQGNRLVLEVAQHLGENTVRCIAMDATEGLVRGQEVADTDAPIQVPVGAATLGRIMNVIGEPVDELGPIEGEALRGIHQPAPSYAEQATEAEILVTGIKVVDLLAPYSKGGKVGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESNVNK DPHENNGSAAGSKCALVYGQMNEPPGARARVALTGLTVAEHFRDQGQDVLFFVDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGQLQERITTTTKGSITSVQAIYVPADDLTDPAPAASFAHLDATTVLSRSIAEKGIYPAVDPLDSTSRMLSPAILGDEHYNTARQVQQTLQRYKALQDIIAILGMDELSEEDKLTVAR ARKIERFLSQPFHVAEVFTGSPGKLVDLADTIKGFKGLVDGKYDYLPEQAFYMVGTIEEAIEKGKKLAAEAA

SEQ ID NO: 34:

Nucleotide sequence of the gamma chain of ATP synthase atpG_2

ATGGCGAGTCTGAAGGACCTGAGAAACCGCATTGCCTCGGTGAAGGCGACGCAGAAGATCACCAAGGCGATGCAGATGGTCGCCGCGGCGAAGCTGCGTCGCGCCCAGGCGGCGGCTGAAGCGGCCCGTCCCTATGCGGAACGCATGGAGACGGTGCTCGGAAATCTTGCCTCCGGCATGGTGGTGGGGCCGCAGGCGCCTGTTCTCATGACCGGGACGGGCAAGAGCGACACCCACCTGCTG CTGGTGTGCACCGGCGAGCGCGGCCTGTGCGGCGCCTTCAACTCGTCCATCGTGCGCTTCGCCCGCGAGCGGGCGCAGCTGCTGCTGGCCGAGGGCAAGAAGGTGAAAATCCTGTGCGTGGGCCGCAAGGGCCACGAGCAGCTGCGCCGCATCTACCCGGACAACATCATCGACGTGGTGGACCTGCGCGCGGTGCGCAACATCGGCTTCAAGGAGGCCGACGCCATCGCCCGCAAGGTGCTGG CCCTGCTCGATGAAGGCGCATTCGACGTCTGCACGCTCTTCTACTCCCACTTCAGGAGCGTGATCGCCCAGGTGCCGACGGCCCAGCAGCTCATTCCGGCCACCTTCGACGAGCGGCCGGCCGTCGCCGATGCGCCGGTCTATGAATATGAGCCGGAGGAGGAGGAGATCCTCGCCGAGCTGCTGCCGCGCAACGTGGCGGTGCAGATCTTCAAGGCCCTCCTCGAGAACCAGGCTTCTTTC TATGGCTCCCAGATGAGCGCCATGGACAACGCCACGCGCAATGCGGGCGAGATGATCAAGAAGCAGACGCTCACCTACAACCGTACCCGCCAGGCCATGATCACGAAGGAACTCATCGAGATCATCTCCGGCGCCGAGGCCGTCTGA

SEQ ID NO: 35:

Amino acid sequence of the gamma chain of ATP synthase atpG_2

MASLKDLRNRIASVKATQKITKAMQMVAAAKLRRAQAAAEAARPYAERMETVLGNLASGMVVGAQAPVLMTGTGKSDTHLLLVCTGERGLCGAFNSSIVRFARERAQLLLAEGKKVKILCVGRKGHEQLRRIYPDNIIDVVDLRAVRNIGFKEADAIARKVLALLDEGAFDVCTLFYSHFRSVIAQVPTAQQLIPATFDERPAVADAPV YEYEPEEEEILAELLPRNVAVQIFKALLENQASFYGSQMSAMDNATRNAGEMIKKQTLTYNRTRQAMITKELIEIISGAEAV

SEQ ID NO: 36:

Nucleotide sequence of the alpha subunit of ATP synthase atpA_2

ATGGACATTCGAGCCGCTGAAAATCTCTGCCATCCTGAAAGAGCAGATCCAGAATTTCGGCCAGGAGGCGGAAGTCTCCGAGGTGGGTCAGGTTCTGTCCGTGGGTGACGGCATCGCGCGTCTACGGCCTCGACAACGTCCAGGCGGGCGAGATGGTCGAGTTCGAGAACGGCACGCGCGGCATGGCGCTGAACCTCGAGCTCGACAATGTCGGCATCGTGATCTTCGGTTCCGACCGCGAGA TCAAGGAAGGCCAGACCGTCAAGCGGACCGGCGCCATCGTGGACGCCCCCGTCGGCAAGGGCCTGCTCGGCCGTCGTGGACGCTCTCGGCAACCCGATCGACGGCAAGGGCCCGATCATGTTCACCGAGCGTCGCCGGGTCGACGTGAAGGCGCCGGGCATCATCCCGCGCAAGTCGGTGCACGAGCCCATGCAGACCGGCCTGAAGGCCATCGATGCGCTCATCCCCCATCGCCGCGGCCAGCG CGAGCTCATCATCGGCGACCGCCAGACCGGCAAGACCGCCGTGGCGCTCGACTCGATCCTGAACCAGAAGCCCATCAACCAGGGCGACGACGAGAAGGCCAAGCTCTACTGCGTCTATGTCGCGGTGGGCCAGAAGCGTTCCACTGTCGCGCAGTTCGTGAAGGTGCTCGAGGAGCACGGCGCGCTGGAATATTCCATCGTCGTCGCCGCCACCGCCTCGGACGCGGCCCCCATGCAGTTCCTGGC GCCGTTCACCGGCACCGCCATGGGCGAGTATTTCGCGCGACAACGGCATGCACGCCCTCATCATCCATGATGACCTGTCCAAGCAGGCCGTGGCCTACCGCCAGATGTCGCTGCTGCTGCGCCGCCCGCCGGGCCGCGAGGCCTATCCCGGCGATGTGTTCTACCTGCACTCCCGCCTCTTGGAGCGCGCCGCCAAGCTCAATGACGAGCACGGCGCGGCTCGCTGACCGCCCTGCCGGTGAT CGAGACCCAGGCCAACGACGTGTCGGCCTACATCCCGACCAACGTGATCTCCATCACCGACGGTCAGATCTTCCTTGAATCCGATCTGTTCTACCAGGGCATCCGCCCGGCGGTGAACGTGGGCCTGTCGGTGTCGCGCGTGGGCTCTTCGGCCCAGATCAAGGCGATGAAGCAGGTGGCCGGCAAGATCAAGGGCGAGCTCGCCCAGTATCGCGAGCTGGCGGCCTTCGCCCAGTTCGG TTCGGACCTGGACGCGGCCACCCAGAAGCTGCTGAACCGCGGCGCCCGCCTCACCGAGCTGCTGAAGCAGAGCCAGTTCTCGCCCCTCAAGGTGGAGGAGCAGGTGGCGGTGATCTATGCCGGCACCAATGGCTATCTCGATCCGCTGCCGGTCTCCAAGGTGCGCGAGTTCGAGCAGGGTCTGCTCCTGTCGCTGCGCTCGCAGCATCCGGAGATCCTGGACGCCATCCGCACGTCCAAGGA GCTTTCCAAGGACACCGCCGAGAAGCTGACGAAGGCCATCGACGCCTTCGCCAAGAGCTTCTCCTGA

SEQ ID NO: 37:

Amino acid sequence of the alpha subunit of ATP synthase atpA_2

MDIRAAEISAILKEQIQNFGQEAEVSEVGQVLSVGDGIARVYGLDNVQAGEMVEFENGTRGMALNLELDNVGIVIFGSDREIKEGQTVKRTGAIVDAPVGKGLLGRVVDALGNPIDGKGPIMFTERRRVDVKAPGIIPRKSVHEPMQTGLKAIDALIPIGRGQRELIIGDRQTGKTAVALDSILNQKPINQGDDEKA KLYCVYVAVGQKRSTVAQFVKVLEEHGALEYSIVVAATASDAAPMQFLAPFTGTAMGEYFRDNGMHALIIHDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDEHGAGSLTALPVIETQANDVSAYIPTNVISITDGQIFLESDLFYQGIRPAVNVGLSVSRVGSSAQIKAMKQVAGKIKGELA QYRELAAFAQFGSDLDAATQKLLNRGARLTELLKQSQFSPLKVEEQVAVIYAGTNGYLDPLPVSKVREFEQGLLLSLRSQHPEILDAIRTSKELSKDTAEKLTKAIDAFAKSFS

SEQ ID NO: 38:

Nucleotide sequence of the delta subunit of ATP synthase atpH

GTGGCGGAAACGATCGTGTCAGGCATGGCGGGACGCTATGCGACCGCGCTGTTCGAGCTGGCGGACGAAGCCGGTGCCATCGATTCCGTCCAGGCGGATCTTGATCGCCTGTCCGGCCTTCTGGCCGAGAGCGCGGATCTGGCGCGGCTGGTCAAGAGCCCGGTCTTCACCGCCGAGCAGCAGCTCGGCGCGATGGCGGCCATTCTCGATCAAGCAGGCATTTCCGGCCTTGCGGGCAAATT CGTGAAGCTGGTGGCGCAGAACCGCCGCCTGTTCGCACTGCCGCGCATGATTGCCGAATACGCCGTCCTGGTGGCCCGGAAGAAGGGCGAGACCTCGGCGAGCGTGACCGTTGCCACCCCCCTGAGCGATGAGCATCTGGCCACGCTCAAGGCGGCCCTGGCTGAAAAGACCGGCAAGGACGTGAAGCTCGACGTCACCGTCGATCCGTCCATCCTCGGTGGTCTCATCGTGAAGCTCGGCTCG CGCATGGTCGATGCTTCCCTGAAGACCAAACTCAATTCTATCCGCATGCGATGAAAGAGGTCCGCTGA

SEQ ID NO: 39:

Amino acid sequence of the delta subunit of ATP synthase atpH

MAETIVSGMAGRYATALFELADEAGAIDSVQADLDRLSGLLAESADLARLVKSPVFTAEQQLGAMAAILDQAGISGLAGKFVKLVAQNRRLFALPRMIAEYAVLVARKKGETSASVTVATPLSDEHLATLKAALAEKTGKDVKLDVTVDPSILGGLIVKLGSRMVDASLKTKLNSIRHAMKEVR

SEQ ID NO: 40:

Nucleotide sequence of subunit b of ATP synthase atpF_2

ATGACCGAAATGGAACTGGCTGAGCTCTGGGTCGCCATCGCCTTCCTGGTTTTCGTAGGCCTCCTGATCTATGCGGGCGCCCACCGCGCCATCGTCTCCGCCCTGGATTCCCGCGGCTCGCGCATCGCCTCGGAACTGGAGGAGGCCCGTCGGCTCAAGGAAGAGGCCCAGAAGCTGGTGGCCGAATTCAAGCGCAAGCAGCGCGAGGCCGAGGCCGAGGCCGAATCCATCGTCACCGGCGCCAAGGCCGAGGCCGAGCGCCTCGCCGCCGAGGCCAAGGCGAAGATCGAGGATTTCGTCACCCGCCGCACCAAGATGGCCGAGGACAAGATCGCCCAGGCCGAGCATCAGGCTCTGGCGGACGTGAAGTCCATCGCCGCCGAGGCGGCGGCCAAGGCGGCCGAGGTGATCCTCGGCGCCCAGGCCACCGGCGCGGTGGCGGAGCGTCTGCTGTCGGGCGCCATCTCCGAGGTCAAGACCAAGCTCAACTGA

SEQ ID NO: 41:

Amino acid sequence of subunit b of ATP synthase atpF_2

MTEMELAELWVAIAFLVFVGLLIYAGAHRAIVSALDSRGSRIASELEEARRLKEEAQKLVAEFKRKQREAEAEAESIVTGAKAEAERLAAEAKAKIEDFVTRRTKMAEDKIAQAEHQALADVKSIAAEAAAKAAEVILGAQATGAVAERLLSGAISEVKTKLN

SEQ ID NO:42:

Nucleotide sequence of subunit b' of ATP synthase atpG_3

ATGATGATTGCATGGAAGCGGACCTTCGCAGTCGTGACCTTCGGGGCCGCCCTGATGGCCATGCCCGTCGCGGGCGTGGTCGCAGCTGAGACTTCTCCCGCTCCGGCGGCAGTGGCGCAGGCCGATCATGCGGTGCCCACCGAGGCGGCCGGCCAGGGCACCGCCGATGCGGCCCATGCCGCCGCGCCGGGCGAGGCCGCCCATGGTGGCGCGCCAAGCACGAAACCCATTTCCCGCCCTTC GACGGCACCACCTTCGCCTCCCAGTTGCTGTGGCTCGCCGTCACCTTCGGCCTGCTTTACTACCTCATGAGCAAGGTCACGCTGCCGCGCATCGGCCGCATCCTGGAAGAGCGCCACGACCGCATCGCCGATGATCTGGAGGAAGCCTCCAAGCATCGCGCCGAGAGCGAGGCCGCCCAGCGGGCCTATGAGAAGGCGCTGAGCGAGGCCCGCGCGAAGGCCCATTCCATCGCCGCGGAAACCCGCGACCGCC TTGCCGCCCACGCCGACACCAACCGCAAGGCGCTGGAGAGCGAGCTCACCGCCAAGCTGCAGGCGGCCGAGGAGCGCATCGCCACCACCAAGAGCGAAGCCCTCACCCATGTGCGCGGCATCGCGGTGGACGCCACCCAATCCATCGTCTCCACCCTCATCGGTGTCGCGCCCGCGGCGGCCGACGTGGAAAAAGCGGTGGACGGCGCCCTGTCCCAGCACGGCCCAGGCCTGA

SEQ ID NO:43:

Amino acid sequence of subunit b' of ATP synthase atpG_3

MMIAWKRTFAVVTFGAALMAMPVAGVVAAETSPAPAAVAQADHAVPTEAAGQGTADAAHAAAPGEAAHGGAAKHETHFPPFDGTTFASQLLWLAVTFGLLYYLMSKVTLPRIGRILEERHDRIADDLEEASKHRAESEAAQRAYEKALSEARAKAHSIAAETRDRLAAHADTNRKALESELTAKLQAAEERIATTKSEALTHVRGIAVDATQ SIVSTLIGVAPAAADVEKAVDGALSQHGQA

SEQ ID NO:44:

Nucleotide sequence of subunit c of ATP synthase atpE_2

ATGGAAGCGGAAGCTGGAAAGTTCATCGGTGCCGGCCTCGCCTGCCTCGGCATGGGTCTCGCTGGCGTCGGCGTCGGTAACATCTTCGGTAACTTCCTCTCCGGCGCCCTGCGCAACCCGTCCGCTGCCGACGGCCAGTTCGCCCGCGCCTTCATCGGCGCCGCCCTCGCGGAAGGTCTCGGCATCTTCTCGCTGGTCGTTGCGCTCGTCCTGCTGTTCGTGGCCTGA

SEQ ID NO: 45:

Amino acid sequence of subunit c of ATP synthase c atpE_2

MEAEAGKFIGAGLACLGMGLAGVGVGNIFGNFLSGALRNPSAADGQFARAFIGAALAEGLGIFSLVVALVLLFVA

SEQ ID NO:46:

Nucleotide sequence of subunit a of ATP synthase atpB_2

ATGACCGTCGATCCGATCCACCAGTTCGAGATCAAGCGCTACGTGGATCTGCTGAACGTCGGCGGTGTCCAGTTCTCCTTCACCAACGCAACGGTGTTCATGATTGGCATCGTCCTGGTGATTTTCTTCTTCCTGACTTTCGCGACACGCGGTCGCACCCTTGTGCCGGGCCGGATGCAGTCGGCGGCGGAGCTGAGCTACGAGTTCATCGCCAAGATGGTGCGCGACGCGGCCGGCA GCGAGGGAATGGTGTTCTTTCCCTTCGTCTTTCTCGCTCTTCATGTTCGTGCTGGTGGCGAACGTATTGGGGCTCATCCCCTACACCTTCACGGTGACCGCCCACCTCATCGTCACCGCCGCCCTGGCGGCGACGGTGATCCTCACCGTCATCATCTACGGCTTCGTGCGGCACGGCACCCACTTCCTGCACCTGTTCGTGCCGTCGGGCGTGCCGGGCTTCCTCCTGCCCTTCCTCGTG GTGATCGAGGTGGTGTCGTTCCTGTCGCGGCCCATCAGCCTCTCGCTGCGTCTGTTCGCCAACATGCTGGCGGGCCACATCGCCCTCAAGGTGTTCGCCTTCTTCGTCGTGGGACTGGCCTCGGCCGGCGCGATCGGCTGGTTCGGCGCCACCCTGCCCTTCTTCATGATCGTGGCGCTCACCGCGCTGGAGCTGCTGGTGGCGGTGCTGCAGGCCTACGTGTTCGCGGTGCTGACC TCGATCTACCTCAACGACGCCATCCATCCCGGCCACTGA

SEQ ID NO:47:

Amino acid sequence of subunit a of ATP synthase atpB_2

MTVDPIHQFEIKRYVDLLNVGGVQFSFTNATVFMIGIVLVIFFFLTFATRGRTLVPGRMQSAAELSYEFIAKMVRDAAGSEGMVFFPFVFSLFMFVLVANVLGLIPYTFTVTAHLIVTAALAATVILTVIIYGFVRHGTHFLHLFVPSGVPGFLLPFLVVIEVVSFLSRPISLSLRLFANMLAGHIALKVFAFFVVGLASAGAIGWFGATLP FFMIVALTALELLVAVLQAYVFAVLTSIYLNDAIHPGH

SEQ ID NO:48:

Nucleotide sequence of ATP synthase protein I atpI

ATGTCCGAGCCGAATGATCCATCCCGCAGGGACGGTGCGAAGGCGAAAGACGAGACGCAGGACTCCCGGCCCGGTGAGGCGGATCTTGCTCGGCGCCTCGATGCGCTCGGCACCTCCATCGGTCAGGTCAAGTCCAGAAGCGGGGAGCCCGCGGCGACGCCGCGCAAGGACACCTCCTCGGCCTCCGGCGCGCGGCCCTGGCGTTTCGGCTGGGCGCCGAGTTTGTTTCAGGCGTGCTGGTG GGCTCGCTCATCGGCTACGGGTTGGATTATGCGTTTGCGATTTCGCCCTGGGGGCTGATCGCCTTCACGCTGATCGGCTTTGCCGCCGGCGTCCTGAACATGCTGCGCGTGGCGAACAGCGATGCCAAGCGCCACAGCGCGGACAGGTGA

SEQ ID NO:49:

Amino acid sequence of ATP synthase protein I atpI

MSEPNDPSRRDGAKAKDETQDSRPGEADLARRLDALGTSIGQVKSRSGEPAATPRKDTSSASGAALAFRLGAEFVSGVLVGSLIGYGLDYAFAISPWGLIAFTLIGFAAGVLNMLRVANSDAKRHSADR

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the molybdenum-iron alpha chain of the nitrogenase protein nifD_1 having the sequence set forth in SEQ ID NO:51, or a sequence having greater than 60% identity, e.g. 70% identity, for example more than 92% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:51 .

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the molybdenum-iron alpha chain of the nitrogenase protein nifD_2 having the sequence specified in SEQ ID NO:53, or a sequence having more than 60% identity, for example more 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:53.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the beta chain of the molybdenum-iron nitrogenase protein nifK_1 having the sequence specified in SEQ ID NO:55, or a sequence having more than 87% identity, for example more 90% identity, for example more than 95% identity, for example more than 96% identity, for example more than 97% identity, for example more than 98%, for example more than 99% sequence identity with the sequence presented in SEQ ID NO:55.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the molybdenum-iron beta chain of the nifK_2 nitrogenase protein having the sequence set forth in SEQ ID NO: 57, or a sequence having greater than 95% identity, e.g. 96% identity, eg greater than 97% identity, eg greater than 98%, eg greater than 99% sequence identity to the sequence shown in SEQ ID NO:57.

In another embodiment, the bacterial strain used in the method of the present invention contains a gene encoding the iron nitrogenase protein nifH having the sequence set forth in SEQ ID NO: 59, or a sequence having greater than 98.5% sequence identity to the sequence set forth in SEQ ID NO: 59.

SEQ ID NO: 50:

Nucleotide sequence of the molybdenum-iron alpha chain of the nitrogenase protein nifD_1

ATGAGTTCGCTCTCCGCCACTATTCAACAGGTCTTCAACGAGCCGGGCTGCGCGAAGAACCAGAATAAGTCCGAGGCGGAGAAGAAGAAGGGCTGCACCAAGCAGCTGCAACCCGGCGGAGCGGCCGGCGGCTGCGCGTTCGACGGCGCGAAGATCGCGCTCCAGCCCTTGACCGACGTCGCCCACCTGGTGCACGGCCCCATCGCCTGCGAAGGCAATTCCTGGGACAATCGTGGCGCCAAG TCCTCCGGCTCGAACATCTGGCGCACCGGCTTCACCACGGACATCAACGAAACCGACGTGGTGTTCGGCGGCGAGAAGCGTCTGTTCAAGTCCATCAAGGAAATCATCGAGAAGTACGACCCGCCGGCCGTCTTCGTCTATCAGACCTGCGTCCCCCGCCATGATCGGCGACGACATCGACGCGGTGTGCAAGGCGGCCAGGAGAAGTTCGGAAAGCCGGTGATCCCGATCAATTCCCCCGGCT TCGTGGGGCCGAAGAATCTCGGCAACAAGCTCGCCGGCGAGGCGCTCCTCGACCATGTGATCGGCACCGAGGAGCCCGATTACACGACGGCCTACGACATCAACATCATCGGCGAATACAATCTCTCCGGCGAGTTGTGGCAGGTGAAGCCGCTGCTGGACGAGCTGGGCATCCGCATCCTCGCCTGCATCTCCGGCGACGGGAAGTACAAGGATGTGGCGTCCTCCCACCGCGCCAAGGCGGC GATGATGGTGTGCTCCAAGGCCATGATCAACGTGGCCCGCAAGATGGAGGAGCGCTACGACATCCCCTTCTTCGAAGGCTCCTTCTACGGCATCGAGGATAGCTCCGATTCCCTGCGCGAGATTGCGCGCATGCTCATCGAGAAGGGCGCCGATCCGGAGCTGATGGACCGCACCGAGGCGCTGATTGAGCGGGAAGAGAAGAAGGCGTGGGACGCCATCGCCGCCTACAAGCCCCGCTTCAAGGACA AGAAGGTGCTGCTCATCACCGGCGGCGTGAAATCCTGGTCGGTGGTGGCAGCGCTCCAGGAAGCCGGCCTCGAACTGGTGGGCACCTCGGTGAAGAAGTCCACCAAGGAGGACAAGGAGCGCATCAAGGAACTGATGGGCCAGGACGCCCACATGATCGACGACATGACGCCCCGCGAAATGTACAAGATGCTGAAGGACGCCAAGGCGGACATCATGCTCGGGCGGGCGCTCGCAATTCATCGCGCT CAAGGCCGCCATGCCCTGGCTCGACATCAACCAGGAGCGCCACCACGCCTATATGGGCTATGTGGGCATGGTGAAGCTGGTCGAGGAGATCGACAAGGCGCTCTACAATCCCGTGTGGGAACAGGTGCGCAAGCCCGCCCCGTGGGAAAATCCGGAAGACACCTGGCAGGCCCGTGCGCTCGCCGAAATGGAGGCGGAGGCCGCCGCGCTCGCCGCCGATCCGGTGCGCGCGGAAGAGGTGCG CCGGTCCAAGAAGATCTGCAATTGCAAGAGCGTCGACCTCGGAACCATTGAGGACGCCATCAAGGCTCACGCGCTGACCACCGTGGAGGGTGTGCGAGAGCACACCAATGCCTCGGGAGGCTGCGGAGCCTGCAGCGGGCGGATCGAGGAGATCTTCGAGGCCGTGGGCGTTGTCGCCGCCCCGCCTCCCGCGGAGGCCGCCCCGTCTCCGCAGGAGATCGCGCCCGATCCGCTCGCTGCGGA GGAAAAGCGCCGCCAAGAAGGCCTGCGGCTGCAAGGAGGTAGCGGTCGGCACCATTGAGGATGCCATCCGCGCCAAGGGTCTGCGAAACATCGCGGAGGTGCGTGCGGCCACCGATGCCAACACCGGCTGCGGCAATTGCCAGGAGCGGGTGGAGGGCATCCTCGACCGGGTTCTCGCCGAGGCGGCCTCAGAACTCCAGGCGGCGGAATAG

SEQ ID NO:51:

Amino acid sequence of the molybdenum-iron alpha chain of the nitrogenase protein nifD_1

MSSLSATIQQVFNEPGCAKNQNKSEAEKKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDVAHLVHGPIACEGNSWDNRGAKSSGSNIWRTGFTTDINETDVVFGGEKRLFKSIKEIIEKYDPPAVFVYQTCVPAMIGDDIDAVCKAAREKFGKPVIPINSPGFVGPKNLGNKLAGEALLDHVIGTEEPDYTTAYDINIIG EYNLSGELWQVKPLLDELGIRILACISGDGKYKDVASSHRAKAAMMVCSKAMINVARKMEERYDIPFFEGSFYGIEDSSDSLREIARMLIEKGADPELMDRTEALIEREEKKAWDAIAAYKPRFKDKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKSTKEDKERIKELMGQDAHMIDDMTPREMYKMLKDAKADIMLSGGRSQ FIALKAAMPWLDINQERHHAYMGYVGMVKLVEEIDKALYNPVWEQVRKPAPWENPEDTWQARALAEMEAEAAALAADPVRAEEVRRSKKICNCKSVDLGTIEDAIKAHALTTVEGVREHTNASGGCGACSGRIEEIFEAVGVVAAPPPAEAAPSPQEIAPDPLAAEEKRRAKKACGCKEVAVGTIEDAIRAKGLRNIAEVRAATDANTGCG NCQERVEGILDRVLAEAASELQAAE

SEQ ID NO:52:

Nucleotide sequence of the molybdenum-iron alpha chain of the nitrogenase protein nifD_2

ATGAGTGTCGCACAGTCCCAGAGCGTCGCCGAGATCAAGGCGCGCAACAAGGAACTCATCGAAGAGGTCCTCAAGGTCTATCCCGAGAAGACCGCCAAGCGCCGCGCCAAGCACCTGAACGTCCACGAAGCCGGCAAGTCCGACTGCGGCGTGAAGTCCAACATCAAGTCCATCCCGGGCGTGATGACCATCCGCGGTTGCGCTTATGCCGGCTCCAAGGGTGTGGTGTGGGGTCCCATCA AGGACATGATCCACATCTCCCACGGCCCGGTGGGCTGCGGCCAGTATAGCTGGGCCGCCCGCCGCAACTATATCGGCACGACCGGCATCGACACCTTCGTGACGATGCAGTTCACCTCCGACTTCCAGGAGAAGGACATCGTCTTCGGCGGCGACAAGAAGCTCGCCAAGATCATGGACGAGATCCAGGAGCTGTTCCCGCTGAACAACGGCATCACCGTTCAGTCCGAGTGCCCCATCGGCCT CATCGGCGACGACATCGAGGCCGTCTCCAAGCAGAAGTCCAAGGAGTATGAGGGCAAGACCATCGTGCCGGTGCGCTGCGAGGGCTTCCGCGGCGTGTCCCAGTCCCTGGGCCACCACATCGCCAACGACGCCATCCGCGATTGGGTGTTCGACAAGATCGCGCCCGACGCCGAGCCGCGCTTTGAGCCGACCCCGTACGACGTCGCCATCATCGGCGACTACAATATCGGTGGTGACGCCTGGTCG TCCCGTATCCTCCTGGAGGAGATGGGCCTGCGCGTGATCGCCCAGTGGTCCGGCGACGGTTCGCTCGCTGAGCTGGAGGCCACCCCGAAGGCCAAGCTCAACGTGCTGCACTGCTACCGCTCCATGAACTACATCTCGCGCCACATGGAAGAGAAGTACGGTATCCCGTGGTGCGAGTACAACTTCTTCGGTCCTTCCAAGATCGCCGAGTCCCTGCGCAAGATCGCCAGCTACTTCGAC GACAAGATCAAGGAAGGCGCGGAGCGCGTCATCGCCAAGTATCAGCCGCTCATGGATGCGGTGATCGCGAAGTATCGTCCCCGCCTCGAGGGCAAGACCGTGATGCTGTACGTGGGCGGCCTGCGTCCCCGTCACGTCATCGGCGCCTACGAGGACCTGGGCATGGAAGTGGTCGGCACGGGCTACGAGTTCGCCCATAACGACGACTACCAGCGCACCGCCCAGCACTACGTCAAGG ATGGCACCATCATCTATGAACGACGTGACCGGCTACGAGTTCGAGAAGTTCGTCGAGAAGATCCAGCCGGACCTGGTCGGTTCGGGCATCAAGGAAAAGTACGTCTTCCAGAAGATGGGCGTGCCGTTCCGCCAGATGCACTCCTGGGACTACTCGGGCCCGTACCACGGCTATGACGGCTTCGCGATCTTCGCGCGCGACATGGACATGGCCATCAACAGCCCCGTGTGGAAGATGACCCAGGCTCC GTGGAAGAGCGTCCCCAAGCCGACGATGCTCGCGGCTGAATGA

SEQ ID NO:53:

Amino acid sequence of the molybdenum-iron alpha chain of the nitrogenase protein nifD_2

MSVAQSQSVAEIKARNKELIEEVLKVYPEKTAKRRAKHLNVHEAGKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIQELFPLNNGITVQSECPIGLIGDDIEAVSKQKSKEYEGKTIVPVR CEGFRGVSQSLGHHIANDAIRDWVFDKIAPDAEPRFEPTPYDVAIIGDYNIGGDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKYGIPWCEYNFFGPSKIAESLRKIASYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFAHND DYQRTAQHYVKDGTIIYDDVTGYEFEKFVEKIQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWKMTQAPWKSVPKPTMLAAE

SEQ ID NO:54:

Nucleotide sequence of the molybdenum-iron beta chain of the nitrogenase protein nifK_1

ATGGCCACCGTTTCCGTCTCCAAGAAGGCCTGCGCGGTCAACCCCCTCAAGATGAGCCAGCCGGTGGGCGGCGCGCTCGCCTTCATGGGCGTGCGCAAGGCCATGCCGCTGCTGCACGGCTCGCAGGGCTGCACCTCCTTCGGCCTGGTGCTGTTCGTGCGCCACTTCAAGGAAGCCATCCCCCATGCAGACCACCGCCATGAGCGAGGTGGCGACGGTTCTGGGCGGCCTTGAGAATGTGGA GCAGGCCATTCTCAACATCTACAATCGCACCAAGCCGGAGATCATCGGCATCTGCTCCACCGGCGTCACCGAGACCAAGGGCGATGATGTCGACGGCTACATCAAGCTGATCCGGGACAAGTATCCCCAGCTGGCCGACTTCCCGCTGGTCTATGTCTCCACCCCCGATTTCAAGGACGCCTTCCAGGACGGTTGGGAGAAGACCGTGGCGAAGATGGTGGAGGCGCTGGTGAAGCCCGCCGCCGACA AGCAGAAGGACAAGACCCGCGTCAACGTCCTGCCCGGCTGCCACCTCACGCCCGGCGATCTGGATGAGATGCGGACCATCTTCGAGGATTTCGGGCTCACACCCTATTTCCTGCCGGATCTGGCCGGCTCGCTGGATGGGCATATCCCCGAGGACTTCTCGCCCACCACCATCGGCGGCATCGGCATCGATGAGATCGCCACCATGGGCGAGGCGGCCCACCATCTGCATCGGCGCGCAGATGCGC CGGGCGGGCGAGGCCATGGAGAAGAAGACCGGCATTCCCTTCAAGCTGTTCGAGCGCCTGTGCGGCCTGGAGGCGAACGACGCCTTCATCATGCACCTGTCGCAGATCTCCGGCCGGCCGGTGCCGGTGAAGTATCGCCGGCAGCGGGGCCAGCTGGTGGATGCCATGCTGGACGGCCACTTCCATCTGGGCGGTCGCAAGGTGGCCATGGGGGCGGAGCCGGACCTGCTCTACGACGTGGGCTC CTTCCTGCACGAGATGGGCGCCCACATCCTTTCCGCGGTCACCACCACCCAGTCGCCGGTGCTGGCGCGCCTGCCTGCCGAGGAGGTGCTTATCGGCGACCTGGAGGATCTGGAGACCCAGGCGAAGGCGCGCGGATGCGATCTCCTGCTCACCCATTCCCATGGGCGCCAGGCGGCGGAGCGCCTCCACATCCCCTTCTACCGGATCGGCATTCCCATGTTTGACCGGCTGGGGGCGGGGCATCT GTTGTCGGTGGGCTATCGCGGCACCCGCGACCTCATCTTCCATCTCGCCAACCTTGTGATCGCCGACCACGAGGAAAATCACGAGCCGACGCCCGACACCTGGGCCACCGGCCATGGCGAGCATGCCGCCGCCCCCACTTCCCATTGA

SEQ ID NO: 55:

Amino acid sequence of the molybdenum-iron beta chain of the nitrogenase protein nifK_1

MATVSVSKKACAVNPLKMSQPVGGALAFMGVRKAMPLLHGSQGCTSFGLVLFVRHFKEAIPMQTTAMSEVATVLGGLENVEQAILNIYNRTKPEIIGICSTGVTETKGDDVDGYIKLIRDKYPQLADFPLVYVSTPDFKDAFQDGWEKTVAKMVEALVKPAADKQKDKTRVNVLPGCHLTPGDLDEMRTIFE DFGLTPYFLPDLAGSLDGHIPEDFSPTTIGGIGIDEIATMGEAAHTICIGAQMRRAGEAMEKKTGIPFKLFERLCGLEANDAFIMHLSQISGRPVPVKYRRQRGQLVDAMLDGHFHLGGRKVAMGAEPDLLYDVGSFLHEMGAHILSAVTTTQSPVLARLPAEEVLIGDLEDLETQAKARGCDLLLTHSHGRQAAERLHIPFYRIGIPMFDRLGAGHLL SVGYRGTRDLIFHLANLVIADHEENHEPTPDTWATGHGEHAAAPTSH

SEQ ID NO:56:

Nucleotide sequence of the molybdenum-iron beta chain of the nitrogenase protein nifK_2

ATGCCACAAAATGCTGACAATGTGCTCGATCACTTCGAGCTCTTCCGTGGTCCCGAATACCAGCAGATGCTGGCCAATAAGAAAAAGATGTTCGAGAACCCCCGCGATCCGGCCGAAGTCGAGCGCGTGCGGGAATGGGCGAAGACTCCTGAATACAAGGAGCTGAACTTCGCCCGCGAGGCGCTCACCGTGAATCCGGCCAAGGCTTGTCAGCCGCTGGGCGCGGTGTTCGTCGCCGTC GGCTTCGAGAGCACGATCCCCTTCGTGCACGGCTCGCAGGGTTGCGTCGCGTATTACCGCTCGCACCTCTCCCGCCACTTCAAGGAGCCGTCCTCCTGCGTCTCCTCGTCCATGACCGAGGATGCGGCGGTGTTCGGCGCCTCAACAACATGATTGACGGCCTCGCCAACACCTACAACATGTACAAGCCGAAGATGATCGCCGTCTCCACCACCTGCATGGCGGAAGTCATCGGCGACG ATCTGAACGCCTTCATCAAGACCGCGAAGGAAAAGGGCTCGGTTCCGGCCGAATACGACGTGCCCTTCGCCCACACCCCGGCGTTCGTCGGCAGCCATGTCACCGGCTACGACAATGCGCTCAAGGGCATCCTCGAGCACTTCTGGGACGGCAAGGCCGGCACCGCGCCGAAGCTGGAGCGCGTTCCCAACGAGAAGATCAACTTCATCGGCGGCTTCGACGGCTACACCGTCGGCAACACTCGCGA AGTGAAGCGCATCTTCGAGGCGTTCGGCGCCGATTACACCATCCTCGCCGACAATTCCGAAGTGTTCGACACCCCGACCGACGGCGAGTTCCGCATGTATGACGGCGGCACGACCCTGGAGGACGCGGCGAACGCGGTGCACGCCAAGGCCACCATCTCCATGCAGGAATACTGCACGGAGAAGACCCTGCCCATGATCGCCGGTCATGGCCAGGACGTGGTCGCCCTCAACCACCCCGTGGGCG TGGGCGGCACCGACAAGTTCCTCATGGAGATCGCCCGCCTCACCGGCAAGGAGATCCCCGAGGAGCTGACCCGCGAGCGCGGCCGTCTCGTGGACGCTATCGCGGACTCTTCCGCGCACATCCACGGCAAGAAGTTCGCCATCTACGGCGATCCGGATCTGTGCCTGGGCCTCGCCGCGTTCCTGCTGGAGCTGGGCGCCGAGCCGACCCATGTGCTGGCCACCAACGGCACCAAGAAGTGGG CCGAGAAGGTTCAGGAACTGTTCGACTCTTCGCCGTTCGGCGCCAACTGCAAGGTCTATCCCGGCAAGGACCTGTGGCACATGCGCTCGCTCCTGTTCGTGGAGCCGGTGGATTTCATCATCGGCAACACCTACGGCAAGTATCTCGAGCGCGACACGGGCACCCCGCTGATCCGTATCGGCTTCCCGGTGTTCGACCGTCACCACCACCACCGCCGTCCGGTGTGGGGCTATCAGGGCGG CATGAACGTCCTGATCACGATCCTCGACAAGATCTTTGACGAGATCGACCGCAACACCAACGTGCCGGCCAAGACCGACTACTCGTTCGACATCATTCGTTGA

SEQ ID NO:57:

Amino acid sequence of the molybdenum-iron beta chain of the nitrogenase protein nifK_2

MPQNADNVLDHFELFRGPEYQQMLANKKKMFENPRDPAEVERVREWAKTPEYKELNFAREALTVNPAKACQPLGAVFVAVGFESTIPFVHGSQGCVAYYRSHLSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANTYNMYKPKMIAVSTTCMAEVIGDDLNAFIKTAKEKGSVPAEYDVPFAHTPAFVGSHVTGYDNALKGILEHF WDGKAGTAPKLERVPNEKINFIGGFDGYTVGNTREVKRIFEAFGADYTILADNSEVFDTPTDGEFRMYDGGTTLEDAANAVHAKATISMQEYCTEKTLPMIAGHGQDVVALNHPVGVGGTDKFLMEIARLTGKEIPEELTRERGRLVDAIADSSAHIHGKKFAIYGDPDLCLGLAAFLLELGAEPTHVLATNGTKKWAEKV QELFDSSPFGANCKVYPGKDLWHMRSLLFVEPVDFIIGNTYGKYLERDTGTPLIRIGFPVFDRHHHHRRPVWGYQGGMNVLITILDKIFDEIDRNTNVPAKTDYSFDIIR

SEQ ID NO:58:

Nucleotide sequence of the iron nitrogenase protein nifH

GTGGAGTCCGGTGGTCCTGAGCCGGGCGTGGGCTGCGCCGGCCGCGGCGTGATCACCTCCATCAACTTCCTGGAGGAGAACGGCGCCTACGAGGACATCGACTATGTGTCCTACGACGTGCTGGGCGACGTGGTGTGCGGCGGCTTCGCCATGCCCATCCGCGAGAACAAGGCGCAGGAAATCTACATCGTGATGTCCGGCGAGATGATGGCCATGTATGCGGCCAACAACATCTCAAGGGCAT CCTGAAGTATGCCAATTCCGGCGGCGTGCGCCTGGGCGGGCTGGTCTGCAACGAGCGCCAGACCGACAAGGAGCTGGAGCTGGCGGAGGCTCTGGCGAAGAAGCTCGGCACCGAGCTGATCTACTTCGTGCCGCGCGACAACATCGTGCAGCATGCCGAGCTGCGCCGCATGACAGTGATCGAGTATGCGCCCGATTCCGCCCAGGCCCAGCACTACCGGAACCTGGCCGAGAAGGTGCACGCC AACAAGGGCAACGGCATCATCCCGACCCCGATCACCATGGACGAGCTGGAAGACATGCTCATGGAGCACGGCATCATGAAGGCCGTGGACGAGAGCCAGATCGGCAAGACCGCCGCCGAGCTCGCCGTCTGA

SEQ ID NO:59:

Amino acid sequence of iron nitrogenase protein nifH

MESGGPEPGVGCAGRGVITSINFLEENGAYEDIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRLGGLVCNERQTDKELELAEALAKKLGTELIYFVPRDNIVQHAELRRMTVIEYAPDSAQAQHYRNLAEKVHANKGNGIIPTPITMDELEDMLMEHGIMKAVDESQIGKTAAEL AV

Further processing

In one embodiment, the method of the present invention includes the additional step of collecting biomass obtained during cultivation. Biomass can, for example, be collected by sedimentation (settling by gravity), filtration, centrifugation or flocculation. Flocculation may require the addition of a flocculant. Centrifugation can be carried out, for example, using a continuous centrifuge.

In one embodiment of the invention, the collected biomass is then dried. Drying can be accomplished, for example, using well known methods including centrifugation, drum drying, evaporation, freeze drying, heat, spray drying, vacuum drying and/or vacuum filtration. The dried biomass can subsequently be used in a product, for example as a food or feed product or a feed or food ingredient.

In another embodiment of the invention, the cells of the collected biomass are lysed. In some embodiments, the lysate may be separated into insoluble and soluble fractions, each or both of which may subsequently be concentrated or dried and then used in a product, such as a food or feed product.

In one embodiment of the invention, biomass is collected and proteins are separated from said biomass, resulting in a protein fraction and a fraction containing non-protein components. Thus, in one embodiment of the invention, the method is for the production of protein and includes the step of cultivating strain VTT-E-193585 or a derivative thereof, followed by the step of collecting biomass and the additional step of isolating proteins from said biomass. In another embodiment of the invention, the method is for producing protein and includes cultivating a bacterial strain of the genus Xanthobacter in a continuous culture with hydrogen as an energy source and an inorganic carbon source, where the inorganic carbon source includes carbon dioxide, followed by the step of collecting biomass, and further stage of protein isolation from the specified biomass. Depending on the method of protein isolation, the resulting fractions may be more or less pure. Thus, the term "protein fraction" means a fraction enriched in proteins. The protein fraction may still contain significant amounts of other components, and significant amounts of protein may also end up in the “non-protein component containing fraction.”

Protein isolation can be carried out using any suitable method. For example, in one embodiment of the invention, proteins are isolated by mechanically disrupting cells and separating the protein from cellular debris using one or more filtration steps, such as sequential filtration through multiple filters of decreasing pore size. Mechanical disruption can be carried out using any suitable method, such as ball milling, sonication, homogenization, high pressure homogenization, mechanical cutting, etc. The resulting filtered protein fraction will be enriched in proteins, but will also contain other smaller components . The protein may optionally be further purified from this fraction using any suitable method.

In another embodiment of the invention, the protein fraction is isolated by ethanol extraction followed by one or more filtration steps. Such methods are known, for example, for the production of soybean proteins (see, for example, Chapter 5 “Soybean Protein Concentrates” in “Technology of production of edible flours and protein products from soybeans” by Berk FAO Agricultural Services Bulletin No. 97 (1992)) . The resulting protein fraction will be enriched with proteins, but also contain other components. The protein may optionally be further purified from this fraction using any suitable method.

In one embodiment, the method of the present invention includes the additional step of hydrolyzing the protein fraction obtained by the method of the present invention to produce amino acids and small peptides.

In one embodiment of the method of the present invention, the method includes the additional step of producing a food product or feed product from said biomass, from said protein fraction, or from said fraction containing non-protein components. Said additional step may simply include introducing said biomass, protein fraction or fraction containing non-protein components into the food or feed product by adding them during production of the food or feed product. In other embodiments of the invention, further purification or modification of the biomass or its fraction is carried out during its incorporation into a food or feed product.

In yet another aspect, the present invention relates to a product, such as biomass, protein or non-protein components, obtained or obtained by the method of the present invention.

In one embodiment of the invention, the product obtained by the method of the present invention contains more than 40% protein, for example, from 40% to 99% protein, for example, from 40% to 90% protein, for example, from 40% to 60% protein. In a specific embodiment of the invention, the product contains from 25% to 75% protein, from 0% to 20% lipids and from 5% to 40% carbohydrates. In another embodiment of the invention, the product contains from 40 to 60% protein, from 0 to 15% lipids and from 10 to 25% carbohydrates. In yet another embodiment of the invention, the product obtained by the method of the present invention contains from 45% to 55% protein, from 5% to 10% lipids and from 10% to 20% carbohydrates.

As described above, in a further aspect, the present invention relates to a food product or feed product produced or produced by the method of the present invention. The terms "food" and "feed" as used herein are intended to include not only conventional food and feed products such as processed foods, but also related products such as food and feed additives, e.g. protein bars, powders or shakes, meat substitutes, food ingredients, probiotics, prebiotics, nutraceuticals and the like. In some embodiments, said biomass, said protein fraction, or said fraction containing non-protein components is used in the production of a vegetarian or vegan food product.

The invention is further illustrated by the following non-limiting examples:

EXAMPLES

Example 1. Isolation of a bacterial strain capable of chemoautotrophic growth

A 50 ml sample containing soil and seawater was collected in a sterile Falcon tube from the Baltic Sea shore at Naantali in Finland. A portion of the soil sample was mixed with 10 ml of mineral medium in a sterile Erlenmeyer flask. The medium contained 1 g/l NH ₄ OH, 0.23 g/l KH ₂ PO ₄ , 0.29 g/l Na ₂ HPO ₄ • 2H ₂ O, 0.005 g/l NaVO ₃ • H2O, 0.2 g/ l FeSO ₄ • 7H ₂ O, 0.5 g/l MgSO ₄ • 7 H ₂ O, 0.01 g/l CaSO ₄ , 0.00015 g/l Na ₂ MoO ₄ • 2H ₂ O, 0.005 g/l MnSO ₄ , 0.0005 g/l ZnSO4 • 7H ₂ O, 0.0015 g/l H ₃ BO ₃ , 0.001 g/l CoSO ₄ , 0.00005 g/l CuSO ₄ and 0.0001 g/l NiSO ₄ , and was prepared with tap water. The soil and medium suspension was incubated in a rocking incubator at a temperature of +30°C in a sealed steel box, continuously purged with a gas mixture: 150 ml/min _N2 , 18 ml/min _H2 , 3 ml/min _O2 and 6 ml/min _CO2 . Culture was resumed at seven-day intervals by removing 1 ml of the suspension, which was added under sterile conditions to 9 ml of medium in the Erlenmeyer flask and then placed back into the incubation box. After the fourth dilution, no noticeable soil contamination remained in the suspension. The volume of the cell suspension was increased to 100 ml in order to increase the biomass for cultivation in the bioreactor. The optical density (OD600) of the suspension was 1.53 when inoculated into 190 ml of mineral medium in a 200 ml parallel bioreactor system with 15 vessels (Medicel Explorer, Medicel Oy, Finland). The culture conditions were stirring at 800 rpm, temperature +30°C, and the pH was adjusted to 6.8 with 1 M NaOH. Gas was supplied through a bubbler in the form of a gas mixture consisting of 14 ml/min H ₂ , 3 ml/min O ₂ and 6 ml/min CO ₂ . The head space of the reactor was purged with air at a speed of 300 ml/min. Continuous cultivation was fed with a mineral medium at a rate of 6 ml/h, the cell suspension was taken from the reactor through a capillary, maintaining a constant volume at 200 ml. The cell suspension removed from the reactor was stored at +4°C. A sample was collected from the bioreactor automatically every day and absorbance was measured at 600 nm to monitor growth. After 498 hours of cultivation in a bioreactor under aseptic conditions, samples were taken, the suspension was diluted and sown on plates with an agar mineral medium containing the above minerals and 2% bacteriological agar. The dishes were incubated under the same conditions as described above for Erlenmeyer flasks. Colonies were then picked from the agar plates and streaked onto new agar plates to isolate one microorganism per colony. This operation was repeated twice. Individual colonies were collected and suspended in 200 μl of medium in a 96-well microtiter plate. The suspension was incubated at +30°C and shaken at 625 rpm in a gas-tight EnzyScreen box, which was continuously purged with 150 ml/min N2, 18 ml/min H ₂ , 3 ml/min O ₂ and 6 ml/min CO ₂ . The suspension from one well was transferred to an Erlenmeyer flask and supplemented with fresh medium. The volume was increased until there was enough biomass to carry out cultivation in the bioreactor. The organism was deposited in the VTT culture collection as VTT-E-193585.

Sequencing of the sample's 16S rRNA revealed that the sample contained only one organism. The same sample was used for Illumina NextSeq sequencing, producing 1x150-bp shotgun metagenomic sequences. Using the Unicycler program (Wick et al., 2017 PLoS computer biology 13:e1005595), a de novo assembly was generated for metagenomic sequences consisting of 101 contigs. The total genome length was 4846739 bp, and the GC content was 67.9%. Gene predictions and functional annotations were performed using Prokka (Seemann, 2014 Bioinformatics 30:2068). Genome annotation identified 4429 genes. The Roary pangenomic alignment (Page et al., 2015 Bioinformatics 31:3691) grouped VTT-E-193585 among Xanthobacter species. Thus, the strain was identified as a Xanthobacter sp., the closest genome for which is Xanthobacter tagetidis . An alignment-based calculation of average nucleotide identity considering only orthologous fragments (OrthoANI) (Lee et al., 2016 Int J Syst Evol Microbiol 66:1100) gave the best match of 80.4% to Xanthobacter tagetidis (ATCC 700314; GCF_003667445.1) , whereas the proposed species boundary is 95-96% (see, e.g., Chun et al., 2018 Int J Syst Evol Microbiol, 68: 461-466). Xanthobacter autotrophicus Py2 gave a 79.6% match, while the match for Xanthobacter sp. 91 was 79.0%. Thus, we can conclude that the isolated bacterial strain, deposited as VTT-E-193585, belongs to the phylum: Proteobacteria; to class: Alpha-proteobacteria; and to order: Rhizobiales. The most likely family is Xanthobacteraceae and the genus Xanthobacter . The bacterial strain VTT-E-193585 cannot be unambiguously assigned to any known species.

A search for putative antimicrobial resistance genes was performed. The ABRicate tool ( https://github.com/tseemann/abricate ) was used for genomic searches of Arg-Annot, NCBI, ResFinder, ecOH, Megares, and VFDB databases using blastn or blastp. A threshold of 50% was set for both identity and coverage at both the nucleotide and protein levels. Only two putative antimicrobial resistance genes have been identified. These two genes do not contain amino acid changes associated with antibiotic resistance, and thus a resistant phenotype is not expected.

Example 2: Pilot cultivation and analysis of an isolated bacterial strain

The isolated bacterial strain, deposited as VTT-E-193585, was cultured in a conventional 200-L stirred tank bioreactor (MPF-U, Marubishi Ltd, Japan). Mixing was carried out with Rushton impellers rotating at a speed of 400 rpm. The temperature during cultivation was maintained at +30°C. The pH value was maintained at 6.8 ± 0.2 by adding 8 M NaOH or 3.6 M H ₃ PO ₄ under program control. The cultivation medium contained 1 g/l NH ₄ OH, 0.23 g/l KH ₂ PO ₄ , 0.29 g/l Na ₂ HPO ₄ • 2H ₂ O, 0.005 g/l NaVO ₃ • H ₂ O, 0. 2 g/l FeSO ₄ • 7H ₂ O, 0.5 g/l MgSO ₄ • 7H ₂ O, 0.01 g/l CaSO ₄ , 0.00015 g/l Na ₂ MoO ₄ • 2H ₂ O, 0.005 g /l MnSO ₄ , 0.0005 g/l ZnSO ₄ • 7H ₂ O, 0.0015 g/l H ₃ BO ₃ , 0.001 g/l CoSO ₄ , 0.00005 g/l CuSO ₄ and 0.0001 g/ l NiSO ₄ , and was prepared with tap water. As the main source of energy and carbon, a mixture containing 1.8-10.5 l/min of hydrogen gas, 0.6-2.5 l/min of oxygen gas and 1.8-5 l/min of carbon dioxide gas was constantly supplied. The level of dissolved oxygen was maintained at 7.2 ± 0.5% by adjusting the composition of the gas mixture. The culture inoculum was prepared as described in Example 1. Growth was monitored by manually sampling and analyzing cell density as optical density by measuring absorbance at 600 nm (UV/visible light spectrophotometer Ultrospec 2100 pro, Biochrom Ltd., England) and by Measuring cell dry weight (CDW) by oven drying overnight at 105°C. Optical density was also monitored using an in situ absorbance sensor (Trucell 2, Finesse Ltd, USA). The growth curve of the cultivation is presented in Figure 1. The maximum growth rate in the periodic phase was 0.06 h ^-1 . The maximum cell density was 4.5 g_CDW/L at 92 hours. After 92 hours of cultivation, the supply of fresh culture medium was started, as described above, at a dilution rate of 0.01 h ^-1 . During continuous feeding, cell density averaged 2.9 g_CDW/L. The culture liquid was constantly collected in a cooled (+10°C) tank, from where it was fed in 300 L portions into a continuous centrifugal separator (BTPX-205, Alfa-Laval AB, Sweden). The suspension containing cells collected from the separator was fed into an atmospheric double-drum dryer (Buflovak 6x8 ADDD, Hebeler Process Solutions Llc., USA), heated by steam at a pressure of 4 bar and drums rotating at a speed of 3.5 rpm. This resulted in a dried cell powder with a dry matter content of approximately 96%. The results of the analysis of the dried cell powder are presented in Table 1 for approximate composition, Table 2 for amino acid composition, Table 3 for fatty acid composition and Table 4 for vitamin content. Tests have shown that the dried cell powder has a high protein content with all essential amino acids. It also contains more unsaturated fatty acids than saturated fatty acids and many B vitamins. The peptidoglycan content is only 0.002 mg/g_CDW and the lipopolysaccharide content is 0.01 mg/g_CDW. It is desirable that these concentrations be as low as possible. In comparison, a commercial lactic acid bacteria preparation analyzed at the same time had a peptidoglycan content of 0.244 mg/g_DW and a lipopolysaccharide content of 0.015 mg/g_DW. Cytotoxicity and genotoxicity assays were performed using culture supernatants. No cytotoxicity was observed against human HepG2 or HeLa229 cell lines. No genotoxicity was observed against Escherichia coli strains WP2 trp- or CM871 uvrA recA lexA .

Table 1. Results of analysis of dried cell powder of the isolated bacterial strain deposited as VTT-E-193585

Parameter Method Units Meaning Humidity Drying at 103°C g/100 g 3.3 Protein Kjeldahl (N x 6.25) g/100 g 72.2 Fat Weibull-Stoldt g/100 g 6.0 Saturated fatty acids Calculation based on Table 3 g/100 g 1.8 Monounsaturated fatty acids Calculation based on Table 3 g/100 g 3.8 Polyunsaturated fatty acids Calculation based on Table 3 g/100 g 0.4 Omega 3 fatty acids Calculation based on Table 3 g/100 g <0.01 Omega 6 fatty acids Calculation based on Table 3 g/100 g 0.4 Alimentary fiber Gravimetric g/100 g 10.5 Ash Ashing at 550°C g/100 g 5.8 Glucose HPLC-ELSD g/100 g <0.15 Fructose HPLC-ELSD g/100 g <0.1 Sucrose HPLC-ELSD g/100 g <0.1 Lactose HPLC-ELSD g/100 g <0.25 Maltose HPLC-ELSD g/100 g <0.2 Total sugars Calculation g/100 g <0.8 Carbohydrates Calculation g/100 g 2.2 Energy Calculation kJ/100g 1572 Energy Calculation kcal/100g 373

Table 2. Amino acid composition of the dried cell powder of the isolated bacterial strain deposited as VTT-E-193585

Parameter Method Units Meaning Lysine Ion chromatography % 3.95 Methionine Ion chromatography % 1.60 Cystine Ion chromatography % 0.39 Aspartic acid Ion chromatography % 6.82 Threonine Ion chromatography % 3.47 Serin Ion chromatography % 2.75 Glutamic acid Ion chromatography % 8.84 Proline Ion chromatography % 3.14 Glycine Ion chromatography % 4.40 Alanin Ion chromatography % 6.94 Valin Ion chromatography % 4.96 Isoleucine Ion chromatography % 3.34 Leucine Ion chromatography % 6.08 Tyrosine Ion chromatography % 2.99 Phenylalanine Ion chromatography % 4.58 Histidine Ion chromatography % 1.66 Arginine Ion chromatography % 4.96 Tryptophan HPLC % 1.34

Table 3. Fatty acid composition of the dried cell powder of the isolated bacterial strain deposited as VTT-E-193585

Parameter Method Units Meaning C16:0 (Palmitic acid) GC-MS % 24.8 C16:1 (Palmitoleic acid) GC-MS % 3.0 C18:0 (Stearic acid) GC-MS % 4.4 C18:1n9 (Oleic acid) GC-MS % 59.9 C18:2n6 (Linoleic acid) GC-MS % 6.1 C18:3n3 (alpha-linolenic acid) GC-MS % 0.4

Table 4. Vitamin content of dried cell powder of the isolated bacterial strain deposited as VTT-E-193585

Parameter Method Units Meaning Vitamin A (PE) HPLC IU/100 g <100 Vitamin E (TE) HPLC mg/100 g 0.33 Vitamin D3 HPLC IU/100 g <10 Vitamin D2 HPLC IU/100 g 21.6 Vitamin C HPLC mg/100 g <1 Thiamine chloride hydrochloride LC-MS/MS mg/100 g 0.9 Vitamin B1 (thiamine) Calculation mg/100 g 0.708 Vitamin B2 (riboflavin) HPLC mg/100 g 6.27 Pyridoxine hydrochloride HPLC mg/100 g 3.39 Vitamin B6 (pyridoxine) Calculation mg/100 g 2.79 Vitamin B12 LC-MS/MS µg/100 g 224 Choline chloride LC-MS/MS mg/100 g 14.3 Biotin LC-MS/MS µg/100 g 15.6 Folic acid Microbiological µg/100 g 1270 Niacin (vitamin B3) Microbiological mg/100 g 23.2 Pantothenic acid Microbiological mg/100 g 6.53

Example 3: Cultivation of an isolated bacterial strain on various nitrogen sources

The isolated bacterial strain, deposited as VTT-E-193585, was cultivated in a parallel bioreactor system with 15 200 ml vessels (Medicel Explorer, Medicel Oy, Finland). Mixing was carried out with Rushton impellers rotating at a speed of 800 rpm. The temperature during cultivation was maintained at +30°C. The pH was maintained at 6.8 by adding 1 M NaOH. The cultivation medium contained 0.23 g/l KH ₂ PO ₄ , 0.29 g/l Na ₂ HPO ₄ • 2 H ₂ O, 0.005 g/l NaVO ₃ • H ₂ O, 0.2 g/l FeSO ₄ • 7H2O, 0.5 g/l MgSO ₄ • 7H ₂ O, 0.01 g/l CaSO ₄ , 0.00015 g/l Na ₂ MoO ₄ • 2H ₂ O, 0.005 g/l MnSO ₄ , 0.0005 g /l ZnSO ₄ • 7H ₂ O, 0.0015 g/l H ₃ BO ₃ , 0.001 g/l CoSO ₄ , 0.00005 g/l CuSO ₄ and 0.0001 g/l NiSO ₄ , and was prepared on tap water. In addition, the nitrogen source of the culture was varied such that four cultures contained 18.7 mM NH ₄ OH, four cultures contained 9.34 mM urea (OC(NH ₂ ) ₂ ), four cultures contained 18.7 mM potassium nitrate (KNO3 ) and three cultivations were left without a source of nitrogen in the medium. As the main source of energy and carbon, a mixture containing 22 ml/min of hydrogen gas, 3.2 ml/min of air and 6.4 ml/min of carbon dioxide gas was continuously supplied. Thus, along with air, all cultivation options also received nitrogen gas. Growth was monitored by automatically sampling and analyzing cell density as optical density by measuring absorbance at 600 nm (UV/visible light spectrophotometer Ultrospec 2100 pro, Biochrom Ltd., England). Cultivation growth curves are shown in Fig. 2. Growth on ammonia and urea was comparable. Growth on nitrate or nitrogen gas was clearly slower than on ammonia or urea. By the end of cultivation, growth on nitrates was better than on nitrogen gas as the only nitrogen source. However, growth was also observed in cultures in which nitrogen gas was the only source of nitrogen, demonstrating that the isolated bacterial strain deposited as VTT-E-193585 is capable of nitrogen fixation.

Example 4: Antibiotic Susceptibility Characterization

Susceptibility to the antibiotics gentamicin, kanamycin, streptomycin, tetracycline, ampicillin, ciprofloxacin, colistin and fosfomycin for the isolated bacterial strain deposited as VTT-E-193585 was analyzed in accordance with CLSI standard M07-A111 (Clinical and Laboratory Standards Institute - Dilution methods in Antimicrobial Susceptibility Tests for Bacteria That Grow Under Aerobic Conditions, 11th Ed., CLSI Standard M07, 2018) with the microdilution plate prepared by the present inventors for ampicillin, ciprofloxacin and colistin, with the VetMIC Lact-1 plate (National Veterinary Institute SVA, Uppsala, Sweden) for gentamicin, kanamycin, streptomycin and tetracycline using the broth microdilution method and fosfomycin using the agar dilution method under aerobic conditions at +35 ± 2°C for 48 ± 1 hours s using Mueller-Hinton broth with adjusted cation content (LabM, LAB114, Mg2+ and Ca2+ cations added separately). Escherichia coli strain ATCC 25922 was used as a quality control strain, which was incubated under aerobic conditions at a temperature of +35 ± 2°C for 18 ± 2 hours. The results of determining the sensitivity of strains to antibiotics are presented in Table 5. It was found that the isolated bacterial strain is generally sensitive to antibiotics. For gentamicin, kanamycin, streptomycin, and tetracycline, the minimum inhibitory concentration (MIC) values for VTT-E-193585 were lower than or comparable to E. coli ATCC 25922, while for ampicillin, ciprofloxacin, colistin, and fosfomycin, the MIC values were higher for VTT -E-193585.

Table 5. Minimum inhibitory concentration (MIC, µg/ml) - antibiotic values for strain VTT-E-193585 and Escherichia coli ATCC 25922

VTT-E-193585
48 h ± 1 h E. coli ATCC 25922
18 h ± 2 h Gentamicin 0.5 0.5 Kanamycin 2 4 Streptomycin 0.5 4 Tetracycline 0.25 1 Ampicillin 16 8 Ciprofloxacin 0.06 0.008 Colistin 4 2 Fosfomycin 32 0.5

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LIST OF SEQUENCES

<110> SOLAR FOODS OH

<120> STRAINS AND METHODS FOR OBTAINING PROTEIN OF SINGLE-CELLULAR ORGANISMS OR BIOMASS

<130> Solar002

<160> 59

<170> Patent version 3.5

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<223> New strain

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cttgagagtt tgatcctggc tcagagcgaa cgctggcggc aggcctaaca catgcaagtc 60

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<223> New strain

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<212> Protein

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<223> New strain

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Met Gly Ala Glu Ala Thr Val Gly Gln Ile Thr Asp Ala Lys Lys Arg

1 5 10 15

Tyr Ala Ala Gly Val Leu Lys Tyr Ala Gln Met Gly Tyr Trp Asn Gly

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Asp Tyr Val Pro Lys Asp Thr Asp Leu Leu Ala Val Phe Arg Ile Thr

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Pro Gln Ala Gly Val Asp Pro Val Glu Ala Ala Ala Ala Val Ala Gly

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Glu Ser Ser Thr Ala Thr Trp Thr Val Val Trp Thr Asp Arg Leu Thr

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Ala Ala Asp Val Tyr Arg Ala Lys Ala Tyr Lys Val Glu Pro Val Pro

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Val Phe Ser Phe Lys Pro Leu Lys Ala Ala Arg Leu Glu Asp Met Arg

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Leu Pro Val Ala Tyr Val Lys Thr Phe Arg Gly Pro Pro Thr Gly Ile

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Val Val Glu Arg Glu Arg Leu Asp Lys Phe Gly Arg Pro Leu Leu Gly

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Ala Thr Thr Lys Pro Lys Leu Gly Leu Ser Gly Lys Asn Tyr Gly Arg

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Val Val Tyr Glu Ala Leu Lys Gly Gly Leu Asp Phe Val Lys Asp Asp

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Glu Asn Ile Asn Ser Gln Pro Phe Met His Trp Arg Asp Arg Phe Leu

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Tyr Cys Met Glu Ala Val Asn Lys Ala Gln Ala Glu Thr Gly Glu Val

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Arg Arg Ala Glu Phe Ala Lys Glu Leu Gly Ser Val Val Val Met Val

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Arg Glu Asn Asp Met Ile Leu His Met His Arg Ala Gly His Gly Thr

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Tyr Thr Arg Gln Lys Ser His Gly Val Ser Phe Arg Val Ile Ala Lys

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Thr Trp Gly Glu Val Thr Phe Asn Tyr Ala Ser Thr Asp Thr Ser Asp

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Tyr Val Pro Thr Ala Ser Val Ala

485

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<212> DNA

<213> Unknown

<220>

<223> New strain

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<212> Protein

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<220>

<223> New strain

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Met Arg Ile Thr Gln Gly Ser Phe Ser Phe Leu Pro Asp Leu Thr Asp

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Thr Gln Ile Lys Ala Gln Val Gln Tyr Cys Leu Asp Gln Gly Trp Ala

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Val Ser Val Glu His Thr Asp Asp Pro His Pro Arg Asn Thr Tyr Trp

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Glu Met Trp Gly Pro Pro Met Phe Asp Leu Arg Asp Ala Ala Gly Val

50 55 60

Phe Gly Glu Ile Glu Ala Cys Arg Ala Ala Asn Pro Glu His Tyr Val

65 70 75 80

Arg Val Asn Ala Phe Asp Ser Ser Arg Gly Trp Glu Thr Ile Arg Leu

85 90 95

Ser Phe Ile Val Gln Arg Pro Thr Val Glu Glu Gly Phe Arg Leu Asp

100 105 110

Arg Thr Glu Gly Lys Gly Arg Asn Gln Ser Tyr Ala Met Arg Tyr Arg

115 120 125

Ala Gln Phe Ala Pro Arg

130

<210> 6

<211> 1896

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 6

atgatgccat ctgagccgca cggcgcgggc atgccgcccc cacgggaagc ggccgcggtt 60

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gtgatgacgc agctcgcgcc cggcgatgcg gccatcatcg tcggccggct gcgggcccat 540

cacgcgccca atgccctgcc gctgttccct ggagccggcg tggccggctc cggcctgccc 600

catgcccgga tccgccccag cctggtgatg ccgggacagc ttctgttccg cgaggaccac 660

acgacgccgg gcgccggcat caaggcggca ctcgccctca ccccggacga agtggtgcag 720

aaggtctccg ccgcgcgcct gcgcgggcgg ggtggcgccg gctttcccac cggtctcaaa 780

tggaagctct gccgccagtc gcccgccacc acccgccatg tgatctgcaa tgcggacgag 840

ggcgagcccg gcaccttcaa ggatcgcgtg ctgctcacgc aggcgccgca cctcatgttc 900

gacggcatga ccatcgccgg ctacgccttg ggggcgcggg agggcgtggt ctatctgcgc 960

ggcgagtacg cctatctgtg ggagcctctg catgcggtcc tgcgcgagcg ctatgggctc 1020

gggctcgccg gcgcgaacat cctgggacac gcgggcttcg acttcgacat ccgcatccag 1080

ctgggcgccg gcgcctatat ctgcggcgag gaatccgcgc tggtggaatc gctggaaggc 1140

aagcgcggct cgccccgcga ccgccccccc ttccccaccg tgcgcggcca tctccagcag 1200

cccaccgccg tggacaatgt ggagaccttc gcctgcgccg cccgcatcct ggagatggc 1260

gtggaggcgt tcgcgggcat cggcacgccc gaatccgccg gcacgaagct cctctcggtg 1320

tcgggcgatt gcccgcgccc cggcgtgtat gaggtgccct tcggcctcac ggtgaacgcg 1380

ctgctcgacc ttgtcggcgc gccggacgcc gccttcgtgc agatgggtgg gccgtccggc 1440

caatgcgtgg cgccgaagga ttacggccgc cgcatcgcct tcgaggacct gcccaccggc 1500

ggctcggtga tggtgttcgg cccggggcgc gacgtgctcg ccatggtgcg cgagttcgcg 1560

gatttcttcg ccggcgaatc ctgcggctgg tgcacgccct gccgggtggg caccaccttg 1620

ctcaaggaag agctggacaa gctcctcgcc aaccgcgcca ccctcgccga catccgcgcg 1680

ctggagaccc tggccacgac cgtctcccgc accagccgct gcggcctcgg cgacggcg 1740

cccaacccca tcctttccac catgcgcaac ctgccggaag cctatgaggc gaggctgagg 1800

cccgaagact tcctgccctg ggcctcgctc gacgaggcgc tgaagcccgc catcgtcatc 1860

cagggccgcg cgcccgtgcc gggaggaagag gcatga 1896

<210> 7

<211> 631

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 7

Met Met Pro Ser Glu Pro His Gly Ala Gly Met Pro Pro Pro Arg Glu

1 5 10 15

Ala Ala Ala Val Pro Thr Pro Gln Glu Val Ser Ala Val Val Ala Glu

20 25 30

Val Val Ala Asp Ala Val Ala Ser Val Gly Gly Ala Arg Thr Arg Leu

35 40 45

Met Asp Ile Val Gln Leu Ala Gln Gln Arg Leu Gly His Leu Ser Glu

50 55 60

Glu Thr Met Ala Ala Ile Ala Ala Arg Leu Ala Ile Pro Pro Val Glu

65 70 75 80

Val Ala Asp Met Val Ser Phe Tyr Ala Phe Leu Asn Arg Ala Pro Lys

85 90 95

Gly Arg Tyr His Ile Arg Leu Ser Arg Ser Pro Ile Ser Leu Met Lys

100 105 110

Gly Ala Glu Ala Val Ala Ala Ala Phe Cys Gln Ile Leu Gly Ile Ala

115 120 125

Met Gly Glu Thr Ser Gln Asp Gly Asp Phe Thr Leu Glu Trp Thr Asn

130 135 140

Asp Ile Gly Met Ala Asp Gln Glu Pro Ala Ala Leu Val Asn Gly Thr

145 150 155 160

Val Met Thr Gln Leu Ala Pro Gly Asp Ala Ala Ile Ile Val Gly Arg

165 170 175

Leu Arg Ala His His Ala Pro Asn Ala Leu Pro Leu Phe Pro Gly Ala

180 185 190

Gly Val Ala Gly Ser Gly Leu Pro His Ala Arg Ile Arg Pro Ser Leu

195 200 205

Val Met Pro Gly Gln Leu Leu Phe Arg Glu Asp His Thr Thr Pro Gly

210 215 220

Ala Gly Ile Lys Ala Ala Leu Ala Leu Thr Pro Asp Glu Val Val Gln

225 230 235 240

Lys Val Ser Ala Ala Arg Leu Arg Gly Arg Gly Gly Ala Gly Phe Pro

245 250 255

Thr Gly Leu Lys Trp Lys Leu Cys Arg Gln Ser Pro Ala Thr Thr Arg

260 265 270

His Val Ile Cys Asn Ala Asp Glu Gly Glu Pro Gly Thr Phe Lys Asp

275 280 285

Arg Val Leu Leu Thr Gln Ala Pro His Leu Met Phe Asp Gly Met Thr

290 295 300

Ile Ala Gly Tyr Ala Leu Gly Ala Arg Glu Gly Val Val Tyr Leu Arg

305 310 315 320

Gly Glu Tyr Ala Tyr Leu Trp Glu Pro Leu His Ala Val Leu Arg Glu

325 330 335

Arg Tyr Gly Leu Gly Leu Ala Gly Ala Asn Ile Leu Gly His Ala Gly

340 345 350

Phe Asp Phe Asp Ile Arg Ile Gln Leu Gly Ala Gly Ala Tyr Ile Cys

355 360 365

Gly Glu Glu Ser Ala Leu Val Glu Ser Leu Glu Gly Lys Arg Gly Ser

370 375 380

Pro Arg Asp Arg Pro Pro Phe Pro Thr Val Arg Gly His Leu Gln Gln

385 390 395 400

Pro Thr Ala Val Asp Asn Val Glu Thr Phe Ala Cys Ala Ala Arg Ile

405 410 415

Leu Glu Asp Gly Val Glu Ala Phe Ala Gly Ile Gly Thr Pro Glu Ser

420 425 430

Ala Gly Thr Lys Leu Leu Ser Val Ser Gly Asp Cys Pro Arg Pro Gly

435 440 445

Val Tyr Glu Val Pro Phe Gly Leu Thr Val Asn Ala Leu Leu Asp Leu

450 455 460

Val Gly Ala Pro Asp Ala Ala Phe Val Gln Met Gly Gly Pro Ser Gly

465 470 475 480

Gln Cys Val Ala Pro Lys Asp Tyr Gly Arg Arg Ile Ala Phe Glu Asp

485 490 495

Leu Pro Thr Gly Gly Ser Val Met Val Phe Gly Pro Gly Arg Asp Val

500 505 510

Leu Ala Met Val Arg Glu Phe Ala Asp Phe Phe Ala Gly Glu Ser Cys

515 520 525

Gly Trp Cys Thr Pro Cys Arg Val Gly Thr Thr Leu Leu Lys Glu Glu

530 535 540

Leu Asp Lys Leu Leu Ala Asn Arg Ala Thr Leu Ala Asp Ile Arg Ala

545 550 555 560

Leu Glu Thr Leu Ala Thr Thr Val Ser Arg Thr Ser Arg Cys Gly Leu

565 570 575

Gly Gln Thr Ala Pro Asn Pro Ile Leu Ser Thr Met Arg Asn Leu Pro

580 585 590

Glu Ala Tyr Glu Ala Arg Leu Arg Pro Glu Asp Phe Leu Pro Trp Ala

595 600 605

Ser Leu Asp Glu Ala Leu Lys Pro Ala Ile Val Ile Gln Gly Arg Ala

610 615 620

Pro Val Pro Glu Glu Glu Ala

625 630

<210> 8

<211> 1491

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 8

atgagccggg gatcccccga tgccgggaaa gaccgcacca tgagcgccac cgacggcacc 60

accgcccccc gcaagatcgt catcgatccg gtgacccgcg tggagggcca cggcaaggtc 120

accatccgcc tggatgaagc cggcgcggtg gaggatgcgc gtttccacat cgtggagttc 180

cgcggcttcg agcggttcat ccagggccgg atgtactggg aagtgcccct tatcatccag 240

cggctgtgcg gcatctgccc ggtgagccac catctggcgg cggcgaaagc catggaccag 300

gtggcgggcg tggaccgcgt accgcccacc gccgagaaac tgcgccggct gatgcattat 360

gggcaggtgc tgcaatccaa cgctttgcac atcttccacc tcgcctcgcc cgacctcctg 420

ttcggcttcg acgcgccggc cgagcagcgc aacatcatcg ccgtgctcca gcgttatccg 480

gagatcggca aatgggcgat cttcatcagg aagttcggcc aggaggtcat caaggccacc 540

ggcgggcgca agatccatcc caccagcgcc attcccggcg gggtcaacca gaacctcgcc 600

gtggaggacc gcgacgccct gcgcgccaag gtgggcgaga tcatcagctg gtgcatggcg 660

gcgctggacc atcacaaggc ctatgtggcg gaaaaccggg cgctgcatga cagcttcgcc 720

gccttcccct ccgccttcat gagcctcgtg gggccggatg gcggcatgga cctttatgac 780

ggcaccctgc gggtgatcga tgccgagggc gcccccctca tcgaaggcgc gccgcccgcc 840

tcctaccgcg accacctcat cgaggaggtg cggccctgga gctatctgaa attcccccat 900

ctgcgcgcct tcggccgcga cgatggctgg tatcgggtcg gccccctcgc ccaggtcaat 960

tgcgccgcgt ccatcgacac gccccgcgcc gaggcggccc ggcgggactt catggccgag 1020

ggcggcggca agccggtgca tgccaccctc gcttatcact gggcgcggct catcgtgctg 1080

gtccattgcg cggagaagat cgaacagctg ctgttcgacg acgacctgca aggctgcgat 1140

ctgcgtgcgg agggcacccg gcgcggggaa ggcgtcgcct ggatcgaggc gccgcgcggc 1200

accctcatcc accattacga ggtggacgag aacgaccagg tgcgccgcgc caacctcatc 1260

gtctccacca cccacaataa cgaggccatg aaccgcgccg tgcggcaggt ggcgaagacg 1320

gacctttccg gtcgcgagat caccgaaggg ctgctgaacc atatcgaggt ggccatccgc 1380

gccttcgacc cctgcctgtc ctgcgccacc catgcgctgg gccagatgcc gctgatcgtg 1440

acgcttgaag atgcctccgg cgcagagatc gcccgcggag tgaaggaatg a 1491

<210> 9

<211> 496

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 9

Met Ser Arg Gly Ser Pro Asp Ala Gly Lys Asp Arg Thr Met Ser Ala

1 5 10 15

Thr Asp Gly Thr Thr Ala Pro Arg Lys Ile Val Ile Asp Pro Val Thr

20 25 30

Arg Val Glu Gly His Gly Lys Val Thr Ile Arg Leu Asp Glu Ala Gly

35 40 45

Ala Val Glu Asp Ala Arg Phe His Ile Val Glu Phe Arg Gly Phe Glu

50 55 60

Arg Phe Ile Gln Gly Arg Met Tyr Trp Glu Val Pro Leu Ile Ile Gln

65 70 75 80

Arg Leu Cys Gly Ile Cys Pro Val Ser His His Leu Ala Ala Ala Lys

85 90 95

Ala Met Asp Gln Val Ala Gly Val Asp Arg Val Pro Pro Thr Ala Glu

100 105 110

Lys Leu Arg Arg Leu Met His Tyr Gly Gln Val Leu Gln Ser Asn Ala

115 120 125

Leu His Ile Phe His Leu Ala Ser Pro Asp Leu Leu Phe Gly Phe Asp

130 135 140

Ala Pro Ala Glu Gln Arg Asn Ile Ile Ala Val Leu Gln Arg Tyr Pro

145 150 155 160

Glu Ile Gly Lys Trp Ala Ile Phe Ile Arg Lys Phe Gly Gln Glu Val

165 170 175

Ile Lys Ala Thr Gly Gly Arg Lys Ile His Pro Thr Ser Ala Ile Pro

180 185 190

Gly Gly Val Asn Gln Asn Leu Ala Val Glu Asp Arg Asp Ala Leu Arg

195 200 205

Ala Lys Val Gly Glu Ile Ile Ser Trp Cys Met Ala Ala Leu Asp His

210 215 220

His Lys Ala Tyr Val Ala Glu Asn Arg Ala Leu His Asp Ser Phe Ala

225 230 235 240

Ala Phe Pro Ser Ala Phe Met Ser Leu Val Gly Pro Asp Gly Gly Met

245 250 255

Asp Leu Tyr Asp Gly Thr Leu Arg Val Ile Asp Ala Glu Gly Ala Pro

260 265 270

Leu Ile Glu Gly Ala Pro Pro Ala Ser Tyr Arg Asp His Leu Ile Glu

275 280 285

Glu Val Arg Pro Trp Ser Tyr Leu Lys Phe Pro His Leu Arg Ala Phe

290 295 300

Gly Arg Asp Asp Gly Trp Tyr Arg Val Gly Pro Leu Ala Gln Val Asn

305 310 315 320

Cys Ala Ala Ser Ile Asp Thr Pro Arg Ala Glu Ala Ala Arg Arg Asp

325 330 335

Phe Met Ala Glu Gly Gly Gly Lys Pro Val His Ala Thr Leu Ala Tyr

340 345 350

His Trp Ala Arg Leu Ile Val Leu Val His Cys Ala Glu Lys Ile Glu

355 360 365

Gln Leu Leu Phe Asp Asp Asp Leu Gln Gly Cys Asp Leu Arg Ala Glu

370 375 380

Gly Thr Arg Arg Gly Glu Gly Val Ala Trp Ile Glu Ala Pro Arg Gly

385 390 395 400

Thr Leu Ile His His Tyr Glu Val Asp Glu Asn Asp Gln Val Arg Arg

405 410 415

Ala Asn Leu Ile Val Ser Thr Thr His Asn Asn Glu Ala Met Asn Arg

420 425 430

Ala Val Arg Gln Val Ala Lys Thr Asp Leu Ser Gly Arg Glu Ile Thr

435 440 445

Glu Gly Leu Leu Asn His Ile Glu Val Ala Ile Arg Ala Phe Asp Pro

450 455 460

Cys Leu Ser Cys Ala Thr His Ala Leu Gly Gln Met Pro Leu Ile Val

465 470 475 480

Thr Leu Glu Asp Ala Ser Gly Ala Glu Ile Ala Arg Gly Val Lys Glu

485 490 495

<210> 10

<211> 720

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 10

atgagcgaga cccccttcac ctttaccgtg gacggcatcg cggtcccggc cacccccggc 60

cagagcgtca tcgaggcgtg cgatgcggcg ggcatctata tcccgcgcct gtgccaccac 120

ccggacctgc cgccggcggg ccattgccgg gtgtgcacct gcatcatcga cgggcggccg 180

gccagcgcct gcaccatgcc cgccgccagg ggcatggtgg tggagaacga gacgcccgct 240

ttgctggcgg agcggcgcac gctgatcgag atgctgttcg cggaaggcaa ccatttctgc 300

cagttctgcg aggcgagcgg cgattgcgaa ttgcaggcgc tgggctacct gttcggcatg 360

gtggccccgc ccttccccca tctgtggccg aagcggccgg tggatgccag ccatccggat 420

atctatatcg accacaatcg ctgcatcctg tgctcgcgct gcgtgcgcgc ctcgcgcacc 480

ctggacggca agtccgtgtt cggcttcgag gggcgcggca tcgagatgca tctggcggtg 540

accggcgggc acctggacga cagcgccatc gccgccgccg acagggcggt tgagatgtgc 600

ccggtgggct gcatcgtcct caagcgcacc ggctaccgca cgccctatgg ccggcggcgc 660

tacgacgccg cgcccatcgg ctccgacatc accgcccggc gcggcggcgc gaaggactga 720

<210> 11

<211> 239

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 11

Met Ser Glu Thr Pro Phe Thr Phe Thr Val Asp Gly Ile Ala Val Pro

1 5 10 15

Ala Thr Pro Gly Gln Ser Val Ile Glu Ala Cys Asp Ala Ala Gly Ile

20 25 30

Tyr Ile Pro Arg Leu Cys His His Pro Asp Leu Pro Pro Ala Gly His

35 40 45

Cys Arg Val Cys Thr Cys Ile Ile Asp Gly Arg Pro Ala Ser Ala Cys

50 55 60

Thr Met Pro Ala Ala Arg Gly Met Val Val Glu Asn Glu Thr Pro Ala

65 70 75 80

Leu Leu Ala Glu Arg Arg Thr Leu Ile Glu Met Leu Phe Ala Glu Gly

85 90 95

Asn His Phe Cys Gln Phe Cys Glu Ala Ser Gly Asp Cys Glu Leu Gln

100 105 110

Ala Leu Gly Tyr Leu Phe Gly Met Val Ala Pro Pro Phe Pro His Leu

115 120 125

Trp Pro Lys Arg Pro Val Asp Ala Ser His Pro Asp Ile Tyr Ile Asp

130 135 140

His Asn Arg Cys Ile Leu Cys Ser Arg Cys Val Arg Ala Ser Arg Thr

145 150 155 160

Leu Asp Gly Lys Ser Val Phe Gly Phe Glu Gly Arg Gly Ile Glu Met

165 170 175

His Leu Ala Val Thr Gly Gly His Leu Asp Asp Ser Ala Ile Ala Ala

180 185 190

Ala Asp Arg Ala Val Glu Met Cys Pro Val Gly Cys Ile Val Leu Lys

195 200 205

Arg Thr Gly Tyr Arg Thr Pro Tyr Gly Arg Arg Arg Tyr Asp Ala Ala

210 215 220

Pro Ile Gly Ser Asp Ile Thr Ala Arg Arg Gly Gly Ala Lys Asp

225 230 235

<210> 12

<211> 543

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 12

atggccaagc ccaaactcgc cacctgcgcg ctggccggct gcttcggctg ccacatgtcc 60

ttcctggaca tggacgagcg catcgtcgag ctcatcgacc tggtggacct cgacgtctcg 120

cccctcgacg acaagaaaaa cttcaccggc atggtggaaa tcggcctggt ggaaggcggc 180

tgcgccgacg agcgccatgt gaaggtgctg cgcgagttcc gcgagaaatc ccgcatcctg 240

gtggcggtgg gcgcctgcgc catcaccggc ggcatcccgg cattgcgcaa cctcgccggc 300

ctcgacgaat gcctgaggga agcctacctc accggcccca cggtggaagg cggcgggctc 360

attcccaacg acccggagct gccgctgctg ctggacaagg tctatccggt gcaggacttc 420

gtgaagatcg accatttcct gcccggctgc ccgccctcgg ccgacgccat ctgggcggct 480

ctgaaggcgc tgctgaccgg caccgagccg catctgccct acccgctttt caagtacgaa 540

tga 543

<210> 13

<211> 180

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 13

Met Ala Lys Pro Lys Leu Ala Thr Cys Ala Leu Ala Gly Cys Phe Gly

1 5 10 15

Cys His Met Ser Phe Leu Asp Met Asp Glu Arg Ile Val Glu Leu Ile

20 25 30

Asp Leu Val Asp Leu Asp Val Ser Pro Leu Asp Asp Lys Lys Asn Phe

35 40 45

Thr Gly Met Val Glu Ile Gly Leu Val Glu Gly Gly Cys Ala Asp Glu

50 55 60

Arg His Val Lys Val Leu Arg Glu Phe Arg Glu Lys Ser Arg Ile Leu

65 70 75 80

Val Ala Val Gly Ala Cys Ala Ile Thr Gly Gly Ile Pro Ala Leu Arg

85 90 95

Asn Leu Ala Gly Leu Asp Glu Cys Leu Arg Glu Ala Tyr Leu Thr Gly

100 105 110

Pro Thr Val Glu Gly Gly Gly Leu Ile Pro Asn Asp Pro Glu Leu Pro

115 120 125

Leu Leu Leu Asp Lys Val Tyr Pro Val Gln Asp Phe Val Lys Ile Asp

130 135 140

His Phe Leu Pro Gly Cys Pro Pro Ser Ala Asp Ala Ile Trp Ala Ala

145 150 155 160

Leu Lys Ala Leu Leu Thr Gly Thr Glu Pro His Leu Pro Tyr Pro Leu

165 170 175

Phe Lys Tyr Glu

180

<210> 14

<211> 1013

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 14

tccagacccg ggcaacattg ctccatgtgc tgggcaccct ggccggccgc tggccccata 60

ccctcgcgct ccagcccggc ggggtgaccc gaagcgccga ccagcacgac cgcatgcgcc 120

tgctcgcgac gctgaaggcg gtgcgggcgg cgctggaaga gaccttgttc ggcgcgcctt 180

tggaagaggt ggcggccctg gacggcgccg ccgccgtgga ggcctggcgc gccaacggcc 240

cggaagggga tttccgcctg ttcctggaga tcgccgccga cctggagctg gaccggctcg 300

gccgcgcgca cgaccgcttt ctctccttcg gcgcctacgc ccaggacgag gggcgccttt 360

atggcgccgg caccttcgag gccgggacgg cgggagggct cgatcccaac gccatcaccg 420

aggaccacgc cttcgcccgc atggaggacc gcgcggcgcc ccatgcgccc tttgacggct 480

ccaccttccc cgatgccgac gacaccgagg gctacacctg gtgcaaggcg ccgcgccttg 540

ccggcctgcc cttcgagacc ggcgccttcg cccggcaggt ggtggcgggc catccgctcg 600

cccgggacct cgtgacgcgg gaaggcggca ctgtgcgcag ccgcgtggtc ggccggctgc 660

tggaaaccgc gcgcaccctg atcgccatgg agggctgggt gaaggaactg cggcccgaag 720

ggccctggtg cgcccagggc cacctgcccc aggaaggccg cgccttcggc ctcaccgagg 780

cggcgcgcgg ggcgctcggc cactggatgg tggtggagaa gggccgcatt gcccgctacc 840

agatcatcgc ccccaccacc tggaacttct ccccccgcga cggcgcgggc ctgcccggcc 900

cgctggagac ggccctggtg ggcgcgcccg tgcggcaggg agagacgacg cccgtgagcg 960

tgcagcacat cgtgcgctcc ttcgacccgt gcatggtctg cactgtgcat tga 1013

<210> 15

<211> 485

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 15

Met Ser Ala Glu Thr Arg Arg Leu Val Val Gly Pro Phe Asn Arg Val

1 5 10 15

Glu Gly Asp Leu Glu Val Arg Leu Asp Val Gln Asp Gly Arg Val Gln

20 25 30

Gln Ala Phe Val Ser Ser Pro Leu Phe Arg Gly Phe Glu Arg Ile Leu

35 40 45

Glu Gly Arg Asp Pro Arg Asp Ala Leu Val Ile Ala Pro Arg Ile Cys

50 55 60

Gly Ile Cys Ser Val Ser Gln Ser His Ala Ala Ala Leu Ala Leu Ala

65 70 75 80

Gly Leu Gln Gly Ile Ala Pro Thr His Asp Gly Arg Ile Ala Thr Asn

85 90 95

Leu Ile Val Ala Ala Glu Asn Val Ala Asp His Leu Thr His Phe His

100 105 110

Val Phe Phe Met Pro Asp Phe Ala Arg Ala Val Tyr Glu Asp Arg Pro

115 120 125

Trp Phe Ala Gln Ala Ala Arg Arg Phe Lys Ala Asn Gln Gly Val Ser

130 135 140

Val Arg Arg Ala Leu Gln Thr Arg Ala Thr Leu Leu His Val Leu Gly

145 150 155 160

Thr Leu Ala Gly Arg Trp Pro His Thr Leu Ala Leu Gln Pro Gly Gly

165 170 175

Val Thr Arg Ser Ala Asp Gln His Asp Arg Met Arg Leu Leu Ala Thr

180 185 190

Leu Lys Ala Val Arg Ala Ala Leu Glu Glu Thr Leu Phe Gly Ala Pro

195 200 205

Leu Glu Glu Val Ala Ala Leu Asp Gly Ala Ala Ala Val Glu Ala Trp

210 215 220

Arg Ala Asn Gly Pro Glu Gly Asp Phe Arg Leu Phe Leu Glu Ile Ala

225 230 235 240

Ala Asp Leu Glu Leu Asp Arg Leu Gly Arg Ala His Asp Arg Phe Leu

245 250 255

Ser Phe Gly Ala Tyr Ala Gln Asp Glu Gly Arg Leu Tyr Gly Ala Gly

260 265 270

Thr Phe Glu Ala Gly Thr Ala Gly Gly Leu Asp Pro Asn Ala Ile Thr

275 280 285

Glu Asp His Ala Phe Ala Arg Met Glu Asp Arg Ala Ala Pro His Ala

290 295 300

Pro Phe Asp Gly Ser Thr Phe Pro Asp Ala Asp Asp Thr Glu Gly Tyr

305 310 315 320

Thr Trp Cys Lys Ala Pro Arg Leu Ala Gly Leu Pro Phe Glu Thr Gly

325 330 335

Ala Phe Ala Arg Gln Val Val Ala Gly His Pro Leu Ala Arg Asp Leu

340 345 350

Val Thr Arg Glu Gly Gly Thr Val Arg Ser Arg Val Val Gly Arg Leu

355 360 365

Leu Glu Thr Ala Arg Thr Leu Ile Ala Met Glu Gly Trp Val Lys Glu

370 375 380

Leu Arg Pro Glu Gly Pro Trp Cys Ala Gln Gly His Leu Pro Gln Glu

385 390 395 400

Gly Arg Ala Phe Gly Leu Thr Glu Ala Ala Arg Gly Ala Leu Gly His

405 410 415

Trp Met Val Val Glu Lys Gly Arg Ile Ala Arg Tyr Gln Ile Ile Ala

420 425 430

Pro Thr Thr Trp Asn Phe Ser Pro Arg Asp Gly Ala Gly Leu Pro Gly

435 440 445

Pro Leu Glu Thr Ala Leu Val Gly Ala Pro Val Arg Gln Gly Glu Thr

450 455 460

Thr Pro Val Ser Val Gln His Ile Val Arg Ser Phe Asp Pro Cys Met

465 470 475 480

Val Cys Thr Val His

485

<210> 16

<211> 858

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 16

acgggggagg aagcccgcgc catcttcgac gccatccttg ccggcgttat cgtcctcgac 60

gccctgtgcg tggaaggcgc gctgctgcgc gggccgaacg gcaccgggcg cttccatgtg 120

ctggcgggca cggacacccc caccatcgac tgggcgcggc agctcgccgg catggcgcgc 180

cacgtggtgg cggtgggcac ctgcgccgcc tatgggggcg tgacggcggc gggcatcaac 240

cccaccgatg cctgcggcct ccagttcgac ggacgccgga agggtggggc gctggggggcg 300

gacttccgct cccgctcggg gcttccggtc atcaatgtgg ccggctgccc cacccatccc 360

aactgggtga cggaaaccct gatgctgctc gcctgcggcc tgctgggcga ggccgacctc 420

gacgtctatg gccgcccgcg cttctatgcg gacctgctgg tgcatcacgg ctgcccgcgc 480

aacgaatact atgaatacaa ggcgagcgcc gagaagatga gcgacctcgg ctgcatgatg 540

gagcatctgg gctgcctcgg cacccaggcc cacgccgact gcaacacgcg cctttggaat 600

ggcgagggct cgtgcacccg cggcggctat gcctgcatca actgcacggc gccggaattc 660

gaggagccgg gccacgcctt cctggagacg cccaagatcg gcggcatccc catcggcctg 720

cccaccgaca tgcccaaggc ctggttcatc gccttgtcct ccctcgccaa ggcggcgacg 780

ccggagcggc tgcgcaagaa cgcggtgtcc gaccatgtgg tcacgccgcc cgccgtcaag 840

gacatcaagc ggcgatga 858

<210> 17

<211> 335

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 17

Met Ser Thr Pro Phe Ser Val Leu Trp Leu Gln Ser Gly Gly Cys Gly

1 5 10 15

Gly Cys Thr Met Ser Leu Leu Cys Ala Glu Ala Pro Asp Leu Ala Thr

20 25 30

Thr Leu Asp Ala Ala Gly Ile Gly Phe Leu Trp His Pro Ala Leu Ser

35 40 45

Glu Glu Thr Gly Glu Glu Ala Arg Ala Ile Phe Asp Ala Ile Leu Ala

50 55 60

Gly Val Ile Val Leu Asp Ala Leu Cys Val Glu Gly Ala Leu Leu Arg

65 70 75 80

Gly Pro Asn Gly Thr Gly Arg Phe His Val Leu Ala Gly Thr Asp Thr

85 90 95

Pro Thr Ile Asp Trp Ala Arg Gln Leu Ala Gly Met Ala Arg His Val

100 105 110

Val Ala Val Gly Thr Cys Ala Ala Tyr Gly Gly Val Thr Ala Ala Gly

115 120 125

Ile Asn Pro Thr Asp Ala Cys Gly Leu Gln Phe Asp Gly Arg Arg Lys

130 135 140

Gly Gly Ala Leu Gly Ala Asp Phe Arg Ser Arg Ser Gly Leu Pro Val

145 150 155 160

Ile Asn Val Ala Gly Cys Pro Thr His Pro Asn Trp Val Thr Glu Thr

165 170 175

Leu Met Leu Leu Ala Cys Gly Leu Leu Gly Glu Ala Asp Leu Asp Val

180 185 190

Tyr Gly Arg Pro Arg Phe Tyr Ala Asp Leu Leu Val His His Gly Cys

195 200 205

Pro Arg Asn Glu Tyr Tyr Glu Tyr Lys Ala Ser Ala Glu Lys Met Ser

210 215 220

Asp Leu Gly Cys Met Met Glu His Leu Gly Cys Leu Gly Thr Gln Ala

225 230 235 240

His Ala Asp Cys Asn Thr Arg Leu Trp Asn Gly Glu Gly Ser Cys Thr

245 250 255

Arg Gly Gly Tyr Ala Cys Ile Asn Cys Thr Ala Pro Glu Phe Glu Glu

260 265 270

Pro Gly His Ala Phe Leu Glu Thr Pro Lys Ile Gly Gly Ile Pro Ile

275 280 285

Gly Leu Pro Thr Asp Met Pro Lys Ala Trp Phe Ile Ala Leu Ser Ser

290 295 300

Leu Ala Lys Ala Ala Thr Pro Glu Arg Leu Arg Lys Asn Ala Val Ser

305 310 315 320

Asp His Val Val Thr Pro Pro Ala Val Lys Asp Ile Lys Arg Arg

325 330 335

<210> 18

<211> 870

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 18

gtgaccgagc gcctgtccga cgtcaacgcc cgcatcgcct cggtgcggca gctctcatcg 60

gtcatcacgg ccatgcgggg cattgcggcg gcgcgggcgc gggaggcgcg gggtcggctc 120

gacggcatcc gcgcctatgc gcagaccatc gccgaggcca tcggccatgt gctcgccgtg 180

ctgcccgagg aggcccgcgc ccggtcctcc gggcaccggc atcggggcca tgcggtcatc 240

gccctgtgcg cggagcaggg ctttgccggc gtcttcaacg agcgggtgct ggacgaggcc 300

gcccggctgc tgaccggcgg ggcggggccg gccgagctgc tgctggtggg cgaccggggc 360

ctgatggtgg cccgcgagcg ggggctcgat gtctcctggt cggtgcccat ggtggcccat 420

gcgggccagg cctcggcgct ggcggaccgc atcagcgagg agctctaccg gcggatcgat 480

gcgggacggg tgacgcgggt gtcggtggtg cacgccgagc ccgccgcgtc cgccgccatc 540

gagacggtgg tgaaagtgct ggtgccgttc gacttcgccc gcttccccct ggcgcgggtg 600

gcatccgccc cgctcatgac catgccgccg ccgcggctgc tggcccagct gtcggaggaa 660

tatgtgttcg ccgagctgtg cgaggcgctc accttgtcct tcgcggcgga gaacgaggcc 720

cgcatgcggg ccatgatcgc cgcccgcgcc aatgtggccg ataccctgga gggcctcgtc 780

ggccgcgccc ggcagatgcg cccaggagg atcaccaacg agatcatcga gctggaaggc 840

ggcgccggca gcgcccggca tgcggattga 870

<210> 19

<211> 289

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 19

Met Thr Glu Arg Leu Ser Asp Val Asn Ala Arg Ile Ala Ser Val Arg

1 5 10 15

Gln Leu Ser Ser Val Ile Thr Ala Met Arg Gly Ile Ala Ala Ala Arg

20 25 30

Ala Arg Glu Ala Arg Gly Arg Leu Asp Gly Ile Arg Ala Tyr Ala Gln

35 40 45

Thr Ile Ala Glu Ala Ile Gly His Val Leu Ala Val Leu Pro Glu Glu

50 55 60

Ala Arg Ala Arg Ser Ser Gly His Arg His Arg Gly His Ala Val Ile

65 70 75 80

Ala Leu Cys Ala Glu Gln Gly Phe Ala Gly Val Phe Asn Glu Arg Val

85 90 95

Leu Asp Glu Ala Ala Arg Leu Leu Thr Gly Gly Ala Gly Pro Ala Glu

100 105 110

Leu Leu Leu Val Gly Asp Arg Gly Leu Met Val Ala Arg Glu Arg Gly

115 120 125

Leu Asp Val Ser Trp Ser Val Pro Met Val Ala His Ala Gly Gln Ala

130 135 140

Ser Ala Leu Ala Asp Arg Ile Ser Glu Glu Leu Tyr Arg Arg Ile Asp

145 150 155 160

Ala Gly Arg Val Thr Arg Val Ser Val Val His Ala Glu Pro Ala Ala

165 170 175

Ser Ala Ala Ile Glu Thr Val Val Lys Val Leu Val Pro Phe Asp Phe

180 185 190

Ala Arg Phe Pro Leu Ala Arg Val Ala Ser Ala Pro Leu Met Thr Met

195 200 205

Pro Pro Pro Arg Leu Leu Ala Gln Leu Ser Glu Glu Tyr Val Phe Ala

210 215 220

Glu Leu Cys Glu Ala Leu Thr Leu Ser Phe Ala Ala Glu Asn Glu Ala

225 230 235 240

Arg Met Arg Ala Met Ile Ala Ala Arg Ala Asn Val Ala Asp Thr Leu

245 250 255

Glu Gly Leu Val Gly Arg Ala Arg Gln Met Arg Gln Glu Glu Ile Thr

260 265 270

Asn Glu Ile Ile Glu Leu Glu Gly Gly Ala Gly Ser Ala Arg His Ala

275 280 285

Asp

<210> 20

<211> 1536

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 20

atgagcacgg gcgcgcaagc gagcgaggat tggctcaccc ggagccgggc ggccctggcc 60

gggacgcgcc tttcccagca atcccaatcg gtgggccggg tggaggagat ggccgacggc 120

atcgcccgcg tctccggcct gccggatgtg cggctcgacg agcttctcac cttcgagggc 180

ggccagaccg gctatgccct caccctcgat cgcaccgaga tcgccgtggt gctgctggat 240

gacgcctccg gcgtggaggc gggcgcccgg gtgttcggca ccggcgaggt ggtgaaggtg 300

ccggtggggc cggggctgct gggccgcatc gtcgaccccc tcggccggcc catggaccgc 360

tccgagccgg tggtggcgca ggcgcaccat cccatcgagc ggccggcgcc ggccatcatc 420

gcccgcgacc tggtctcgca gccggttcag accggcacgc tggtggtgga tgcgctgttc 480

tccctcggcc ggggccagcg cgagctcatc atcggcgacc gggctaccgg caagaccgcc 540

atcgcggtgg acaccatcat cagccagaag cattcggaca tcgtgtgcat ctacgtggcg 600

gtgggccagc gcgccgccgc cgtggagcgg gtggtggagg cggtgcgcgc ccacggggcg 660

atcgagcgct gcatcttcgt ggtcgcctcg gccgccgcct cgccagggct gcaatggatc 720

gcgccgttcg ccggcatgac catggcggaa tatttccgcg acaacggcca gcatgcgctc 780

atcatcatcg atgatctcac caagcatgcg gccacccatc gcgagctggc gctgctcacc 840

cacgagccgc cgggccgcga ggcctatccc ggcgacatct tctatgtgca cgcccgcctt 900

ctggagcggg ccgccaagct ctccgccgag ctgggcggtg gctcgctcac ggccctgccc 960

atcgcggaga cggacgcggg aaacctctcc gcctatatcc ccaccaacct catctccatc 1020

accgatgggc agatcgtgct ggattcgcgg ctgttcgcgg ccaaccagcg cccggcggtg 1080

gatgtgggcc tctccgtgag ccgggtgggc ggcaaggcgc agcatcccgc gcttcgggcc 1140

gtgtccgggc gcatccggct cgattattcc cagttcctgg agctggaaat gttcacccgc 1200

ttcggcggca tcaccgatac ccgcgtgaag gcgcagatca cccggggcga gcgcatccgc 1260

gcgctgctca cccagccgcg cttttccacc ctgcgccttc aggacgaggt ggcgctgctg 1320

gccgcgctgg cggagggggt gttcgacact ttggccccgg ggctgatggg cgccgtgcgt 1380

gcccgcattc cggcccagct ggatgcgcag gtgaaggacg tggcctcggc cctcgccgag 1440

ggcaaggtgc tggagggaggg cttgcacgcc cgtctcgtgg cggccgtgcg ggccgtcgcg 1500

gcggacgtgg ccgcgaccgc gaaggccggg ccgtga 1536

<210> 21

<211> 511

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 21

Met Ser Thr Gly Ala Gln Ala Ser Glu Asp Trp Leu Thr Arg Ser Arg

1 5 10 15

Ala Ala Leu Ala Gly Thr Arg Leu Ser Gln Gln Ser Gln Ser Val Gly

20 25 30

Arg Val Glu Glu Met Ala Asp Gly Ile Ala Arg Val Ser Gly Leu Pro

35 40 45

Asp Val Arg Leu Asp Glu Leu Leu Thr Phe Glu Gly Gly Gln Thr Gly

50 55 60

Tyr Ala Leu Thr Leu Asp Arg Thr Glu Ile Ala Val Val Leu Leu Asp

65 70 75 80

Asp Ala Ser Gly Val Glu Ala Gly Ala Arg Val Phe Gly Thr Gly Glu

85 90 95

Val Val Lys Val Pro Val Gly Pro Gly Leu Leu Gly Arg Ile Val Asp

100 105 110

Pro Leu Gly Arg Pro Met Asp Arg Ser Glu Pro Val Val Ala Gln Ala

115 120 125

His His Pro Ile Glu Arg Pro Ala Pro Ala Ile Ile Ala Arg Asp Leu

130 135 140

Val Ser Gln Pro Val Gln Thr Gly Thr Leu Val Val Asp Ala Leu Phe

145 150 155 160

Ser Leu Gly Arg Gly Gln Arg Glu Leu Ile Ile Gly Asp Arg Ala Thr

165 170 175

Gly Lys Thr Ala Ile Ala Val Asp Thr Ile Ile Ser Gln Lys His Ser

180 185 190

Asp Ile Val Cys Ile Tyr Val Ala Val Gly Gln Arg Ala Ala Ala Val

195 200 205

Glu Arg Val Val Glu Ala Val Arg Ala His Gly Ala Ile Glu Arg Cys

210 215 220

Ile Phe Val Val Ala Ser Ala Ala Ala Ser Pro Gly Leu Gln Trp Ile

225 230 235 240

Ala Pro Phe Ala Gly Met Thr Met Ala Glu Tyr Phe Arg Asp Asn Gly

245 250 255

Gln His Ala Leu Ile Ile Ile Asp Asp Leu Thr Lys His Ala Ala Thr

260 265 270

His Arg Glu Leu Ala Leu Leu Thr His Glu Pro Pro Gly Arg Glu Ala

275 280 285

Tyr Pro Gly Asp Ile Phe Tyr Val His Ala Arg Leu Leu Glu Arg Ala

290 295 300

Ala Lys Leu Ser Ala Glu Leu Gly Gly Gly Ser Leu Thr Ala Leu Pro

305 310 315 320

Ile Ala Glu Thr Asp Ala Gly Asn Leu Ser Ala Tyr Ile Pro Thr Asn

325 330 335

Leu Ile Ser Ile Thr Asp Gly Gln Ile Val Leu Asp Ser Arg Leu Phe

340 345 350

Ala Ala Asn Gln Arg Pro Ala Val Asp Val Gly Leu Ser Val Ser Arg

355 360 365

Val Gly Gly Lys Ala Gln His Pro Ala Leu Arg Ala Val Ser Gly Arg

370 375 380

Ile Arg Leu Asp Tyr Ser Gln Phe Leu Glu Leu Glu Met Phe Thr Arg

385 390 395 400

Phe Gly Gly Ile Thr Asp Thr Arg Val Lys Ala Gln Ile Thr Arg Gly

405 410 415

Glu Arg Ile Arg Ala Leu Leu Thr Gln Pro Arg Phe Ser Thr Leu Arg

420 425 430

Leu Gln Asp Glu Val Ala Leu Leu Ala Ala Leu Ala Glu Gly Val Phe

435 440 445

Asp Thr Leu Ala Pro Gly Leu Met Gly Ala Val Arg Ala Arg Ile Pro

450 455 460

Ala Gln Leu Asp Ala Gln Val Lys Asp Val Ala Ser Ala Leu Ala Glu

465 470 475 480

Gly Lys Val Leu Glu Glu Gly Leu His Ala Arg Leu Val Ala Ala Val

485 490 495

Arg Ala Val Ala Ala Asp Val Ala Ala Thr Ala Lys Ala Gly Pro

500 505 510

<210> 22

<211> 741

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 22

atgcagatcg actggtggac gctgggcctg cagacggtca acgtcctcgt tctcatctgg 60

ctcctgagcc gcttcctgtt caagccggtg gcgcaggtca tcgcgcagcg ccgtgccgag 120

atcgagaagc tggtggagga tgcgcgcgcc gccaaggccg ccgccgaggc cgagcgggac 180

acggcgaagg cggaggaggc gcgccttgcc gccgagcgcg gcgcccgcat ggcggcggtc 240

gccaaggagg cggaggcgca gaaggcggca ttgctggccg ccgccaagac cgaggccgag 300

gccctgcacg cggccgcgga agcggccatc gtccgggcgc gggcgagcga ggaggaagcc 360

gccgccgacc gcgccagccg ccttgccgtg gacatcgccg ccaagctgct ggaccggctg 420

cccgacgacg cccgggtcgc gggcttcatc gatggcctcg ccgaggggct tgaagccctg 480

cccgaggcga gccgggcggt gatcggcgtc gacggcgcgc cagtgcgcgt gacggccgcg 540

cgcgccctta tgccggcgga ggaggaggcc tgccgcacgc ggctctccca ggcgctgggc 600

cgtccggtga cgctggccgt gaccatcgac cccgccctca tcgccggcct ggagatggag 660

acgccccacg cggtggtgcg caattccttc aaggccgatc tcgaccgcgt caccgcggcg 720

ctcacccatc atgggacctg a 741

<210> 23

<211> 246

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 23

Met Gln Ile Asp Trp Trp Thr Leu Gly Leu Gln Thr Val Asn Val Leu

1 5 10 15

Val Leu Ile Trp Leu Leu Ser Arg Phe Leu Phe Lys Pro Val Ala Gln

20 25 30

Val Ile Ala Gln Arg Arg Ala Glu Ile Glu Lys Leu Val Glu Asp Ala

35 40 45

Arg Ala Ala Lys Ala Ala Ala Glu Ala Glu Arg Asp Thr Ala Lys Ala

50 55 60

Glu Glu Ala Arg Leu Ala Ala Glu Arg Gly Ala Arg Met Ala Ala Val

65 70 75 80

Ala Lys Glu Ala Glu Ala Gln Lys Ala Ala Leu Leu Ala Ala Ala Lys

85 90 95

Thr Glu Ala Glu Ala Leu His Ala Ala Ala Glu Ala Ala Ile Val Arg

100 105 110

Ala Arg Ala Ser Glu Glu Glu Ala Ala Ala Asp Arg Ala Ser Arg Leu

115 120 125

Ala Val Asp Ile Ala Ala Lys Leu Leu Asp Arg Leu Pro Asp Asp Ala

130 135 140

Arg Val Ala Gly Phe Ile Asp Gly Leu Ala Glu Gly Leu Glu Ala Leu

145 150 155 160

Pro Glu Ala Ser Arg Ala Val Ile Gly Val Asp Gly Ala Pro Val Arg

165 170 175

Val Thr Ala Ala Arg Ala Leu Met Pro Ala Glu Glu Glu Ala Cys Arg

180 185 190

Thr Arg Leu Ser Gln Ala Leu Gly Arg Pro Val Thr Leu Ala Val Thr

195 200 205

Ile Asp Pro Ala Leu Ile Ala Gly Leu Glu Met Glu Thr Pro His Ala

210 215 220

Val Val Arg Asn Ser Phe Lys Ala Asp Leu Asp Arg Val Thr Ala Ala

225 230 235 240

Leu Thr His His Gly Thr

245

<210> 24

<211> 243

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 24

atgactgtcg agatggtcag catcttcgcg gcggcgctcg ccgtctcctt cggcgccatc 60

gggccggccc tgggcgaggg ccgggcggtg gccgcggcca tggacgccat cgcccgccag 120

ccggaggcgg ccggaacctt gtcgcgcacg ctcttcgtcg gcctcgccat gatcgagacc 180

atggcgatct actgcctggt gatcgcgctc ctggtgctct tcgccaatcc gttcgtgaag 240

tga 243

<210> 25

<211> 80

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 25

Met Thr Val Glu Met Val Ser Ile Phe Ala Ala Ala Leu Ala Val Ser

1 5 10 15

Phe Gly Ala Ile Gly Pro Ala Leu Gly Glu Gly Arg Ala Val Ala Ala

20 25 30

Ala Met Asp Ala Ile Ala Arg Gln Pro Glu Ala Ala Gly Thr Leu Ser

35 40 45

Arg Thr Leu Phe Val Gly Leu Ala Met Ile Glu Thr Met Ala Ile Tyr

50 55 60

Cys Leu Val Ile Ala Leu Leu Val Leu Phe Ala Asn Pro Phe Val Lys

65 70 75 80

<210> 26

<211> 699

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 26

atgggctcgc cgctgatcct cgaacccctg ttccatatcg ggcccgtgcc catcaccgcg 60

ccggtggtgg tcacctggct catcatggcc gccttcattg ggctggcgcg gctcatcacc 120

cggaagcttt ccaccgatcc cacccggacc caggcggcgg tggaaacggt gctgaccgcc 180

atcgattccc agatcgccga caccatgcag gccgatcccg cgccttatcg cgcgctcatc 240

ggcaccatct tcctttatgt gctggtggcc aactggtcct cgctcatccc gggcatcgag 300

ccgcccacgg cgcatatcga gaccgatgcg gcgctcgctt tcatcgtgtt cgccgccacc 360

atcgggttcg ggttgaagac aaggggtgtg aagggctatc tcgccacctt cgccgaaccc 420

tcctgggtga tgatcccgct caatgtggtg gagcagatca cccggacctt ctcgctcatc 480

gtgcgcctgt tcggcaacat catgagcggg gtgttcgtgg tcggcatcat cctgtccctc 540

gccgggctgc tggtgcccat ccccctcatg gcgctcgatc tcctgaccgg cgccgtgcag 600

gcctacatct tcgcggtgct ggcctgcgtg ttcatcggcg cggccattgg cgaggcgccg 660

gcaaagcccc aatcgaagga gccagggaaa acatcatga 699

<210> 27

<211> 232

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 27

Met Gly Ser Pro Leu Ile Leu Glu Pro Leu Phe His Ile Gly Pro Val

1 5 10 15

Pro Ile Thr Ala Pro Val Val Val Thr Trp Leu Ile Met Ala Ala Phe

20 25 30

Ile Gly Leu Ala Arg Leu Ile Thr Arg Lys Leu Ser Thr Asp Pro Thr

35 40 45

Arg Thr Gln Ala Ala Val Glu Thr Val Leu Thr Ala Ile Asp Ser Gln

50 55 60

Ile Ala Asp Thr Met Gln Ala Asp Pro Ala Pro Tyr Arg Ala Leu Ile

65 70 75 80

Gly Thr Ile Phe Leu Tyr Val Leu Val Ala Asn Trp Ser Ser Leu Ile

85 90 95

Pro Gly Ile Glu Pro Pro Thr Ala His Ile Glu Thr Asp Ala Ala Leu

100 105 110

Ala Phe Ile Val Phe Ala Ala Thr Ile Gly Phe Gly Leu Lys Thr Arg

115 120 125

Gly Val Lys Gly Tyr Leu Ala Thr Phe Ala Glu Pro Ser Trp Val Met

130 135 140

Ile Pro Leu Asn Val Val Glu Gln Ile Thr Arg Thr Phe Ser Leu Ile

145 150 155 160

Val Arg Leu Phe Gly Asn Ile Met Ser Gly Val Phe Val Val Gly Ile

165 170 175

Ile Leu Ser Leu Ala Gly Leu Leu Val Pro Ile Pro Leu Met Ala Leu

180 185 190

Asp Leu Leu Thr Gly Ala Val Gln Ala Tyr Ile Phe Ala Val Leu Ala

195 200 205

Cys Val Phe Ile Gly Ala Ala Ile Gly Glu Ala Pro Ala Lys Pro Gln

210 215 220

Ser Lys Glu Pro Gly Lys Thr Ser

225 230

<210> 28

<211> 456

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 28

gtgagcgcgc cgctgcacct caccatcacc acgccggccg ccgttctggt ggaccgtgcc 60

gacatcgtgg ccctgcgtgc cgaggacgag agcggcagct tcggcatcct gcccggccat 120

gcggatttcc tgaccgttct ggaggcctgc gtggtgcgct tcaaggatgg ggccgacggc 180

gtgcattatt gtgctctcag tggtggcgtg ctgtcggtcg aggagggccg gcgcatcgcc 240

atcgcctgcc gtcagggcac ggtgagcgac gacctggtcg ccctggaagg ggcggtggac 300

gccatgcgtt cggcggagag cgatgccgac aagcgggccc gggtggagca gatgcgcctt 360

catgcccacg ccgtgcgcca gctcctgcac tatctgcggc ccggccgggc cggcggcgtg 420

gcgccggccg ccgcgccgga ggaggggccg tcatga 456

<210> 29

<211> 151

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 29

Met Ser Ala Pro Leu His Leu Thr Ile Thr Thr Pro Ala Ala Val Leu

1 5 10 15

Val Asp Arg Ala Asp Ile Val Ala Leu Arg Ala Glu Asp Glu Ser Gly

20 25 30

Ser Phe Gly Ile Leu Pro Gly His Ala Asp Phe Leu Thr Val Leu Glu

35 40 45

Ala Cys Val Val Arg Phe Lys Asp Gly Ala Asp Gly Val His Tyr Cys

50 55 60

Ala Leu Ser Gly Gly Val Leu Ser Val Glu Glu Gly Arg Arg Ile Ala

65 70 75 80

Ile Ala Cys Arg Gln Gly Thr Val Ser Asp Asp Leu Val Ala Leu Glu

85 90 95

Gly Ala Val Asp Ala Met Arg Ser Ala Glu Ser Asp Ala Asp Lys Arg

100 105 110

Ala Arg Val Glu Gln Met Arg Leu His Ala His Ala Val Arg Gln Leu

115 120 125

Leu His Tyr Leu Arg Pro Gly Arg Ala Gly Gly Val Ala Pro Ala Ala

130 135 140

Ala Pro Glu Glu Gly Pro Ser

145 150

<210> 30

<211> 1494

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 30

atggcagcgg cagatgagga ggcgcaatcg gccgccggcc ccgcctcggg ccgggtggtg 60

gccgtgcgcg gcgcggtgat cgacatcgcc tttgcccagc ctccgctgcc gccgctggac 120

gacgcccttc tcatcaccga cggccggggc ggcacggtgc tggtggaggt gcagagccat 180

atggatcggc acacggtgcg cgccatcgcc cttcaggcca ccaccggcct cagccggggg 240

ctggaggcgg cgcgggtggg cgggccggtg aaggtgccgg tggggagacca tgtgctcggc 300

cgcctcctgg atgtcaccgg cgccatcggc gacaagggcg ggccgctgcc ggccgacgtg 360

cccacgcggc cgatccacca cgcgccgcca tccttcgccg cgcagggcgg cacgtccgat 420

ctgtttcgca ccggcatcaa ggtcatcgac ctcctggcgc ccctcgccca gggcggcaag 480

gcggccatgt tcggcggggc cggcgtgggc aagaccgtgc tggtgatgga gctgatccac 540

gccatggtgg cgagctacaa gggcatctcg gtgtttgccg gcgtggggga gcgctcccgc 600

gagggccacg agatgctgct ggacatgacc gattccggcg tgctcgaccg caccgttctg 660

gtctatggcc agatgaacga gccccccggg gcccgctggc gggtgcccat gacggcgctg 720

accatcgccg aatatttccg cgacgagaag caccagaacg tcctgctgct gatggacaac 780

atcttccgct tcgtccaggc gggggcggag gtctccggcc ttttgggccg tccgccctcc 840

cgggtgggat accagccgac gctggcgagc gaggtggcgg cgctccagga acgcatcacc 900

tccgtgggcg aggcctcggt gaccgccatc gaggcggtct acgtgccggc ggatgacttc 960

accgatcccg ccgtgaccac catcgccgcc cacgtggatt ccatggtggt gctctcccgc 1020

gccatggcgg cggagggcat gtatccggcg gtggacccca tctcctcctc gtcggtgctg 1080

ctcgacccgc tcatcgtggg ggacgagcat gcgcgcgtcg ccaacgaggt gcgccggacc 1140

atcgagcatt atcgcgagct tcaggatgtg atctcgctgc tgggcatgga ggaattgggc 1200

accgaggatc gccgcatcgt ggagcgggcg cgccggctcc agcgcttcct cacccagccc 1260

ttcacggtca ccgaggcctt caccggcgtg cccggccgct cggtggccat cgccgacacc 1320

atcgccggct gcaggatgat cctgtccggc gcctgcgacg actggcagga aagcgccctc 1380

tacatggtgg gcaccatcga cgaggcccgc cagaaggagg aggccgctcg cgccaaggcg 1440

gggcagggcg ccccggccgg gacggcagcc gagacggcgg aggccgcccc gtga 1494

<210> 31

<211> 497

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 31

Met Ala Ala Ala Asp Glu Glu Ala Gln Ser Ala Ala Gly Pro Ala Ser

1 5 10 15

Gly Arg Val Val Ala Val Arg Gly Ala Val Ile Asp Ile Ala Phe Ala

20 25 30

Gln Pro Pro Leu Pro Pro Leu Asp Asp Ala Leu Leu Ile Thr Asp Gly

35 40 45

Arg Gly Gly Thr Val Leu Val Glu Val Gln Ser His Met Asp Arg His

50 55 60

Thr Val Arg Ala Ile Ala Leu Gln Ala Thr Thr Gly Leu Ser Arg Gly

65 70 75 80

Leu Glu Ala Ala Arg Val Gly Gly Pro Val Lys Val Pro Val Gly Asp

85 90 95

His Val Leu Gly Arg Leu Leu Asp Val Thr Gly Ala Ile Gly Asp Lys

100 105 110

Gly Gly Pro Leu Pro Ala Asp Val Pro Thr Arg Pro Ile His His Ala

115 120 125

Pro Pro Ser Phe Ala Ala Gln Gly Gly Thr Ser Asp Leu Phe Arg Thr

130 135 140

Gly Ile Lys Val Ile Asp Leu Leu Ala Pro Leu Ala Gln Gly Gly Lys

145 150 155 160

Ala Ala Met Phe Gly Gly Ala Gly Val Gly Lys Thr Val Leu Val Met

165 170 175

Glu Leu Ile His Ala Met Val Ala Ser Tyr Lys Gly Ile Ser Val Phe

180 185 190

Ala Gly Val Gly Glu Arg Ser Arg Glu Gly His Glu Met Leu Leu Asp

195 200 205

Met Thr Asp Ser Gly Val Leu Asp Arg Thr Val Leu Val Tyr Gly Gln

210 215 220

Met Asn Glu Pro Pro Gly Ala Arg Trp Arg Val Pro Met Thr Ala Leu

225 230 235 240

Thr Ile Ala Glu Tyr Phe Arg Asp Glu Lys His Gln Asn Val Leu Leu

245 250 255

Leu Met Asp Asn Ile Phe Arg Phe Val Gln Ala Gly Ala Glu Val Ser

260 265 270

Gly Leu Leu Gly Arg Pro Pro Ser Arg Val Gly Tyr Gln Pro Thr Leu

275 280 285

Ala Ser Glu Val Ala Ala Leu Gln Glu Arg Ile Thr Ser Val Gly Glu

290 295 300

Ala Ser Val Thr Ala Ile Glu Ala Val Tyr Val Pro Ala Asp Asp Phe

305 310 315 320

Thr Asp Pro Ala Val Thr Thr Ile Ala Ala His Val Asp Ser Met Val

325 330 335

Val Leu Ser Arg Ala Met Ala Ala Glu Gly Met Tyr Pro Ala Val Asp

340 345 350

Pro Ile Ser Ser Ser Ser Val Leu Leu Asp Pro Leu Ile Val Gly Asp

355 360 365

Glu His Ala Arg Val Ala Asn Glu Val Arg Arg Thr Ile Glu His Tyr

370 375 380

Arg Glu Leu Gln Asp Val Ile Ser Leu Leu Gly Met Glu Glu Leu Gly

385 390 395 400

Thr Glu Asp Arg Arg Ile Val Glu Arg Ala Arg Arg Leu Gln Arg Phe

405 410 415

Leu Thr Gln Pro Phe Thr Val Thr Glu Ala Phe Thr Gly Val Pro Gly

420 425 430

Arg Ser Val Ala Ile Ala Asp Thr Ile Ala Gly Cys Arg Met Ile Leu

435 440 445

Ser Gly Ala Cys Asp Asp Trp Gln Glu Ser Ala Leu Tyr Met Val Gly

450 455 460

Thr Ile Asp Glu Ala Arg Gln Lys Glu Glu Ala Ala Arg Ala Lys Ala

465 470 475 480

Gly Gln Gly Ala Pro Ala Gly Thr Ala Ala Glu Thr Ala Glu Ala Ala

485 490 495

Pro

<210> 32

<211> 1437

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 32

atggcgaaca aggtcggacg catcaccag atcatcggcg ccgtcgtcga cgtgcagttc 60

gacgggcatc tgccggcgat tctcaacgcg atcgagacca ccaaccaggg caaccggctg 120

gtgctcgaag tggctcagca tctcggcgag aacaccgtgc gctgcatcgc catggatgcc 180

actgaaggcc tggtgcgtgg ccaggaggtg gccgacaccg atgcgcccat ccaggtgccc 240

gtgggcgccg ccaccctcgg ccgcatcatg aacgtgatcg gcgagccggt ggacgagctg 300

ggccccatcg agggcgaagc gctgcgcggc atccatcagc cggccccctc ctatgcggag 360

caggccacgg aagctgagat cctcgtcacc ggcatcaagg tggtggatct gctggcgccc 420

tattccaagg gcggcaaggt gggcctgttc ggcggcgccg gcgtgggcaa gaccgtgctc 480

atcatggagc tgatcaacaa cgtggccaag gcgcacggcg gctattccgt gttcgccggc 540

gtgggtgagc gcacccgcga gggcaacgac ctctaccacg agatgatcga gtccaacgtg 600

aacaaggacc cgcacgagaa caatggctcg gcggccggtt ccaagtgcgc cctggtctat 660

ggccagatga acgagccgcc cggcgcccgc gcccgcgtgg ccctcaccgg cctcaccgtc 720

gccgagcatt tccgcgacca gggccaggac gtgctgttct tcgtggacaa catcttccgc 780

ttcacccagg cgggctccga ggtgtcggcg cttctcggcc gcatcccctc ggcggtgggc 840

taccagccga cgctggccac cgacatgggc cagctgcagg agcgcatcac caccaccacc 900

aagggctcca tcacctcggt gcaggccatc tacgtgccgg cggacgatct gaccgatccg 960

gcgccggccg cctccttcgc ccatctggac gccaccacgg tgctgtcgcg ctccatcgcg 1020

gagaagggca tctacccggc ggtggatccg ctggactcca cctcgcgcat gctgtctccc 1080

gccatcctcg gcgacgagca ctacaacacc gcgcgccagg tgcagcagac cctgcagcgc 1140

tacaaggcgc tccaggacat catcgccatc ctgggcatgg acgaactctc cgaagaggac 1200

aagctcaccg tggcccgcgc ccgcaagatc gagcgcttcc tctcccagcc cttccacgtg 1260

gccgaggtgt tcaccggttc gcccggcaag ctggtcgacc tcgccgacac catcaagggc 1320

ttcaagggcc tggtggacgg caagtacgac tacctgcccg agcaggcctt ctacatggtg 1380

ggcaccatcg aagaagccat cgagaagggc aagaagctgg cggccgaggc ggcctga 1437

<210> 33

<211> 478

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 33

Met Ala Asn Lys Val Gly Arg Ile Thr Gln Ile Ile Gly Ala Val Val

1 5 10 15

Asp Val Gln Phe Asp Gly His Leu Pro Ala Ile Leu Asn Ala Ile Glu

20 25 30

Thr Thr Asn Gln Gly Asn Arg Leu Val Leu Glu Val Ala Gln His Leu

35 40 45

Gly Glu Asn Thr Val Arg Cys Ile Ala Met Asp Ala Thr Glu Gly Leu

50 55 60

Val Arg Gly Gln Glu Val Ala Asp Thr Asp Ala Pro Ile Gln Val Pro

65 70 75 80

Val Gly Ala Ala Thr Leu Gly Arg Ile Met Asn Val Ile Gly Glu Pro

85 90 95

Val Asp Glu Leu Gly Pro Ile Glu Gly Glu Ala Leu Arg Gly Ile His

100 105 110

Gln Pro Ala Pro Ser Tyr Ala Glu Gln Ala Thr Glu Ala Glu Ile Leu

115 120 125

Val Thr Gly Ile Lys Val Val Asp Leu Leu Ala Pro Tyr Ser Lys Gly

130 135 140

Gly Lys Val Gly Leu Phe Gly Gly Ala Gly Val Gly Lys Thr Val Leu

145 150 155 160

Ile Met Glu Leu Ile Asn Asn Val Ala Lys Ala His Gly Gly Tyr Ser

165 170 175

Val Phe Ala Gly Val Gly Glu Arg Thr Arg Glu Gly Asn Asp Leu Tyr

180 185 190

His Glu Met Ile Glu Ser Asn Val Asn Lys Asp Pro His Glu Asn Asn

195 200 205

Gly Ser Ala Ala Gly Ser Lys Cys Ala Leu Val Tyr Gly Gln Met Asn

210 215 220

Glu Pro Pro Gly Ala Arg Ala Arg Val Ala Leu Thr Gly Leu Thr Val

225 230 235 240

Ala Glu His Phe Arg Asp Gln Gly Gln Asp Val Leu Phe Phe Val Asp

245 250 255

Asn Ile Phe Arg Phe Thr Gln Ala Gly Ser Glu Val Ser Ala Leu Leu

260 265 270

Gly Arg Ile Pro Ser Ala Val Gly Tyr Gln Pro Thr Leu Ala Thr Asp

275 280 285

Met Gly Gln Leu Gln Glu Arg Ile Thr Thr Thr Thr Lys Gly Ser Ile

290 295 300

Thr Ser Val Gln Ala Ile Tyr Val Pro Ala Asp Asp Leu Thr Asp Pro

305 310 315 320

Ala Pro Ala Ala Ser Phe Ala His Leu Asp Ala Thr Thr Val Leu Ser

325 330 335

Arg Ser Ile Ala Glu Lys Gly Ile Tyr Pro Ala Val Asp Pro Leu Asp

340 345 350

Ser Thr Ser Arg Met Leu Ser Pro Ala Ile Leu Gly Asp Glu His Tyr

355 360 365

Asn Thr Ala Arg Gln Val Gln Gln Thr Leu Gln Arg Tyr Lys Ala Leu

370 375 380

Gln Asp Ile Ile Ala Ile Leu Gly Met Asp Glu Leu Ser Glu Glu Asp

385 390 395 400

Lys Leu Thr Val Ala Arg Ala Arg Lys Ile Glu Arg Phe Leu Ser Gln

405 410 415

Pro Phe His Val Ala Glu Val Phe Thr Gly Ser Pro Gly Lys Leu Val

420 425 430

Asp Leu Ala Asp Thr Ile Lys Gly Phe Lys Gly Leu Val Asp Gly Lys

435 440 445

Tyr Asp Tyr Leu Pro Glu Gln Ala Phe Tyr Met Val Gly Thr Ile Glu

450 455 460

Glu Ala Ile Glu Lys Gly Lys Lys Leu Ala Ala Glu Ala Ala

465 470 475

<210> 34

<211> 876

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 34

atggcgagtc tgaaggacct gagaaaccgc attgcctcgg tgaaggcgac gcagaagatc 60

accaaggcga tgcagatggt cgccgcggcg aagctgcgtc gcgcccaggc ggcggctgaa 120

gcggcccgtc cctatgcgga acgcatggag acggtgctcg gaaatcttgc ctccggcatg 180

gtggtgggcg cgcaggcgcc tgttctcatg accgggacgg gcaagagcga cacccacctg 240

ctgctggtgt gcaccggcga gcgcggcctg tgcggcgcct tcaactcgtc catcgtgcgc 300

ttcgcccgcg agcgggcgca gctgctgctg gccgagggca agaaggtgaa aatcctgtgc 360

gtgggccgca agggccacga gcagctgcgc cgcatctacc cggacaacat catcgacgtg 420

gtggacctgc gcgcggtgcg caacatcggc ttcaaggagg ccgacgccat cgcccgcaag 480

gtgctggccc tgctcgatga aggcgcattc gacgtctgca cgctcttcta ctcccacttc 540

aggagcgtga tcgcccaggt gccgacggcc cagcagctca ttccggccac cttcgacgag 600

cggccggccg tcgccgatgc gccggtctat gaatatgagc cggagggagga ggagatcctc 660

gccgagctgc tgccgcgcaa cgtggcggtg cagatcttca aggccctcct cgagaaccag 720

gcttctttct atggctccca gatgagcgcc atggacaacg ccacgcgcaa tgcgggcgag 780

atgatcaaga agcagacgct cacctacaac cgtacccgcc aggccatgat cacgaaggaa 840

ctcatcgaga tcatctccgg cgccgaggcc gtctga 876

<210> 35

<211> 291

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 35

Met Ala Ser Leu Lys Asp Leu Arg Asn Arg Ile Ala Ser Val Lys Ala

1 5 10 15

Thr Gln Lys Ile Thr Lys Ala Met Gln Met Val Ala Ala Ala Lys Leu

20 25 30

Arg Arg Ala Gln Ala Ala Ala Glu Ala Ala Arg Pro Tyr Ala Glu Arg

35 40 45

Met Glu Thr Val Leu Gly Asn Leu Ala Ser Gly Met Val Val Gly Ala

50 55 60

Gln Ala Pro Val Leu Met Thr Gly Thr Gly Lys Ser Asp Thr His Leu

65 70 75 80

Leu Leu Val Cys Thr Gly Glu Arg Gly Leu Cys Gly Ala Phe Asn Ser

85 90 95

Ser Ile Val Arg Phe Ala Arg Glu Arg Ala Gln Leu Leu Leu Ala Glu

100 105 110

Gly Lys Lys Val Lys Ile Leu Cys Val Gly Arg Lys Gly His Glu Gln

115 120 125

Leu Arg Arg Ile Tyr Pro Asp Asn Ile Ile Asp Val Val Asp Leu Arg

130 135 140

Ala Val Arg Asn Ile Gly Phe Lys Glu Ala Asp Ala Ile Ala Arg Lys

145 150 155 160

Val Leu Ala Leu Leu Asp Glu Gly Ala Phe Asp Val Cys Thr Leu Phe

165 170 175

Tyr Ser His Phe Arg Ser Val Ile Ala Gln Val Pro Thr Ala Gln Gln

180 185 190

Leu Ile Pro Ala Thr Phe Asp Glu Arg Pro Ala Val Ala Asp Ala Pro

195 200 205

Val Tyr Glu Tyr Glu Pro Glu Glu Glu Glu Ile Leu Ala Glu Leu Leu

210 215 220

Pro Arg Asn Val Ala Val Gln Ile Phe Lys Ala Leu Leu Glu Asn Gln

225 230 235 240

Ala Ser Phe Tyr Gly Ser Gln Met Ser Ala Met Asp Asn Ala Thr Arg

245 250 255

Asn Ala Gly Glu Met Ile Lys Lys Gln Thr Leu Thr Tyr Asn Arg Thr

260 265 270

Arg Gln Ala Met Ile Thr Lys Glu Leu Ile Glu Ile Ile Ser Gly Ala

275 280 285

Glu Ala Val

290

<210> 36

<211> 1530

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 36

atggacattc gagccgctga aatctctgcc atcctgaaag agcagatcca gaatttcggc 60

caggaggcgg aagtctccga ggtgggtcag gttctgtccg tgggtgacgg catcgcgcgc 120

gtctacggcc tcgacaacgt ccaggcgggc gagatggtcg agttcgagaa cggcacgcgc 180

ggcatggcgc tgaacctcga gctcgacaat gtcggcatcg tgatcttcgg ttccgaccgc 240

gagatcaagg aaggccagac cgtcaagcgg accggcgcca tcgtggacgc ccccgtcggc 300

aagggcctgc tcggccgcgt cgtggacgct ctcggcaacc cgatcgacgg caagggcccg 360

atcatgttca ccgagcgtcg ccgggtcgac gtgaaggcgc cgggcatcat cccgcgcaag 420

tcggtgcacg agcccatgca gaccggcctg aaggccatcg atgcgctcat ccccatcggc 480

cgcggccagc gcgagctcat catcggcgac cgccagaccg gcaagaccgc cgtggcgctc 540

gactcgatcc tgaaccagaa gcccatcaac cagggcgacg acgagaaggc caagctctac 600

tgcgtctatg tcgcggtggg ccagaagcgt tccactgtcg cgcagttcgt gaaggtgctc 660

gaggagcacg gcgcgctgga atattccatc gtcgtcgccg ccaccgcctc ggacgcggcc 720

cccatgcagt tcctggcgcc gttcaccggc accgccatgg gcgagtattt ccgcgacaac 780

ggcatgcacg ccctcatcat ccatgatgac ctgtccaagc aggccgtggc ctaccgccag 840

atgtcgctgc tgctgcgccg cccgccgggc cgcgaggcct atcccggcga tgtgttctac 900

ctgcactccc gcctcttgga gcgcgccgcc aagctcaatg acgagcacgg cgccggctcg 960

ctgaccgccc tgccggtgat cgagacccag gccaacgacg tgtcggccta catcccgacc 1020

aacgtgatct ccatcaccga cggtcagatc ttccttgaat ccgatctgtt ctaccagggc 1080

atccgcccgg cggtgaacgt gggcctgtcg gtgtcgcgcg tgggctcttc ggcccagatc 1140

aaggcgatga agcaggtggc cggcaagatc aagggcgagc tcgcccagta tcgcgagctg 1200

gcggccttcg cccagttcgg ttcggacctg gacgcggcca cccagaagct gctgaaccgc 1260

ggcgcccgcc tcaccgagct gctgaagcag agccagttct cgcccctcaa ggtggaggag 1320

caggtggcgg tgatctatgc cggcaccaat ggctatctcg atccgctgcc ggtctccaag 1380

gtgcgcgagt tcgagcaggg tctgctcctg tcgctgcgct cgcagcatcc ggagatcctg 1440

gacgccatcc gcacgtccaa ggagctttcc aaggacaccg ccgagaagct gacgaaggcc 1500

atcgacgcct tcgccaagag cttctcctga 1530

<210> 37

<211> 509

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 37

Met Asp Ile Arg Ala Ala Glu Ile Ser Ala Ile Leu Lys Glu Gln Ile

1 5 10 15

Gln Asn Phe Gly Gln Glu Ala Glu Val Ser Glu Val Gly Gln Val Leu

20 25 30

Ser Val Gly Asp Gly Ile Ala Arg Val Tyr Gly Leu Asp Asn Val Gln

35 40 45

Ala Gly Glu Met Val Glu Phe Glu Asn Gly Thr Arg Gly Met Ala Leu

50 55 60

Asn Leu Glu Leu Asp Asn Val Gly Ile Val Ile Phe Gly Ser Asp Arg

65 70 75 80

Glu Ile Lys Glu Gly Gln Thr Val Lys Arg Thr Gly Ala Ile Val Asp

85 90 95

Ala Pro Val Gly Lys Gly Leu Leu Gly Arg Val Val Asp Ala Leu Gly

100 105 110

Asn Pro Ile Asp Gly Lys Gly Pro Ile Met Phe Thr Glu Arg Arg Arg

115 120 125

Val Asp Val Lys Ala Pro Gly Ile Ile Pro Arg Lys Ser Val His Glu

130 135 140

Pro Met Gln Thr Gly Leu Lys Ala Ile Asp Ala Leu Ile Pro Ile Gly

145 150 155 160

Arg Gly Gln Arg Glu Leu Ile Ile Gly Asp Arg Gln Thr Gly Lys Thr

165 170 175

Ala Val Ala Leu Asp Ser Ile Leu Asn Gln Lys Pro Ile Asn Gln Gly

180 185 190

Asp Asp Glu Lys Ala Lys Leu Tyr Cys Val Tyr Val Ala Val Gly Gln

195 200 205

Lys Arg Ser Thr Val Ala Gln Phe Val Lys Val Leu Glu Glu His Gly

210 215 220

Ala Leu Glu Tyr Ser Ile Val Val Ala Ala Thr Ala Ser Asp Ala Ala

225 230 235 240

Pro Met Gln Phe Leu Ala Pro Phe Thr Gly Thr Ala Met Gly Glu Tyr

245 250 255

Phe Arg Asp Asn Gly Met His Ala Leu Ile Ile His Asp Asp Leu Ser

260 265 270

Lys Gln Ala Val Ala Tyr Arg Gln Met Ser Leu Leu Leu Arg Arg Pro

275 280 285

Pro Gly Arg Glu Ala Tyr Pro Gly Asp Val Phe Tyr Leu His Ser Arg

290 295 300

Leu Leu Glu Arg Ala Ala Lys Leu Asn Asp Glu His Gly Ala Gly Ser

305 310 315 320

Leu Thr Ala Leu Pro Val Ile Glu Thr Gln Ala Asn Asp Val Ser Ala

325 330 335

Tyr Ile Pro Thr Asn Val Ile Ser Ile Thr Asp Gly Gln Ile Phe Leu

340 345 350

Glu Ser Asp Leu Phe Tyr Gln Gly Ile Arg Pro Ala Val Asn Val Gly

355 360 365

Leu Ser Val Ser Arg Val Gly Ser Ser Ala Gln Ile Lys Ala Met Lys

370 375 380

Gln Val Ala Gly Lys Ile Lys Gly Glu Leu Ala Gln Tyr Arg Glu Leu

385 390 395 400

Ala Ala Phe Ala Gln Phe Gly Ser Asp Leu Asp Ala Ala Thr Gln Lys

405 410 415

Leu Leu Asn Arg Gly Ala Arg Leu Thr Glu Leu Leu Lys Gln Ser Gln

420 425 430

Phe Ser Pro Leu Lys Val Glu Glu Gln Val Ala Val Ile Tyr Ala Gly

435 440 445

Thr Asn Gly Tyr Leu Asp Pro Leu Pro Val Ser Lys Val Arg Glu Phe

450 455 460

Glu Gln Gly Leu Leu Leu Ser Leu Arg Ser Gln His Pro Glu Ile Leu

465 470 475 480

Asp Ala Ile Arg Thr Ser Lys Glu Leu Ser Lys Asp Thr Ala Glu Lys

485 490 495

Leu Thr Lys Ala Ile Asp Ala Phe Ala Lys Ser Phe Ser

500 505

<210> 38

<211> 555

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 38

gtggcggaaa cgatcgtgtc aggcatggcg ggacgctatg cgaccgcgct gttcgagctg 60

gcggacgaag ccggtgccat cgattccgtc caggcggatc ttgatcgcct gtccggcctt 120

ctggccgaga gcgcggatct ggcgcggctg gtcaagagcc cggtcttcac cgccgagcag 180

cagctcggcg cgatggcggc cattctcgat caagcaggca tttccggcct tgcgggcaaa 240

ttcgtgaagc tggtggcgca gaaccgccgc ctgttcgcac tgccgcgcat gattgccgaa 300

tacgccgtcc tggtggcccg gaagaagggc gagacctcgg cgagcgtgac cgttgccacc 360

cccctgagcg atgagcatct ggccacgctc aaggcggccc tggctgaaaa gaccggcaag 420

gacgtgaagc tcgacgtcac cgtcgatccg tccatcctcg gtggtctcat cgtgaagctc 480

ggctcgcgca tggtcgatgc ttccctgaag accaaactca attctatccg gcatgcgatg 540

aaagaggtcc gctga 555

<210> 39

<211> 184

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 39

Met Ala Glu Thr Ile Val Ser Gly Met Ala Gly Arg Tyr Ala Thr Ala

1 5 10 15

Leu Phe Glu Leu Ala Asp Glu Ala Gly Ala Ile Asp Ser Val Gln Ala

20 25 30

Asp Leu Asp Arg Leu Ser Gly Leu Leu Ala Glu Ser Ala Asp Leu Ala

35 40 45

Arg Leu Val Lys Ser Pro Val Phe Thr Ala Glu Gln Gln Leu Gly Ala

50 55 60

Met Ala Ala Ile Leu Asp Gln Ala Gly Ile Ser Gly Leu Ala Gly Lys

65 70 75 80

Phe Val Lys Leu Val Ala Gln Asn Arg Arg Leu Phe Ala Leu Pro Arg

85 90 95

Met Ile Ala Glu Tyr Ala Val Leu Val Ala Arg Lys Lys Gly Glu Thr

100 105 110

Ser Ala Ser Val Thr Val Ala Thr Pro Leu Ser Asp Glu His Leu Ala

115 120 125

Thr Leu Lys Ala Ala Leu Ala Glu Lys Thr Gly Lys Asp Val Lys Leu

130 135 140

Asp Val Thr Val Asp Pro Ser Ile Leu Gly Gly Leu Ile Val Lys Leu

145 150 155 160

Gly Ser Arg Met Val Asp Ala Ser Leu Lys Thr Lys Leu Asn Ser Ile

165 170 175

Arg His Ala Met Lys Glu Val Arg

180

<210> 40

<211> 492

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 40

atgaccgaaa tggaactggc tgagctctgg gtcgccatcg ccttcctggt tttcgtaggc 60

ctcctgatct atgcgggcgc ccaccgcgcc atcgtctccg ccctggattc ccgcggctcg 120

cgcatcgcct cggaactgga ggaggcccgt cggctcaagg aagaggccca gaagctggtg 180

gccgaattca agcgcaagca gcgcgaggcc gaggccgagg ccgaatccat cgtcaccggc 240

gccaaggccg aggccgagcg cctcgccgcc gaggccaagg cgaagatcga ggatttcgtc 300

acccgccgca ccaagatggc cgaggacaag atcgcccagg ccgagcatca ggctctggcg 360

gacgtgaagt ccatcgccgc cgaggcggcg gccaaggcgg ccgaggtgat cctcggcgcc 420

caggccaccg gcgcggtggc ggagcgtctg ctgtcgggcg ccatctccga ggtcaagacc 480

aagctcaact ga 492

<210> 41

<211> 163

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 41

Met Thr Glu Met Glu Leu Ala Glu Leu Trp Val Ala Ile Ala Phe Leu

1 5 10 15

Val Phe Val Gly Leu Leu Ile Tyr Ala Gly Ala His Arg Ala Ile Val

20 25 30

Ser Ala Leu Asp Ser Arg Gly Ser Arg Ile Ala Ser Glu Leu Glu Glu

35 40 45

Ala Arg Arg Leu Lys Glu Glu Ala Gln Lys Leu Val Ala Glu Phe Lys

50 55 60

Arg Lys Gln Arg Glu Ala Glu Ala Glu Ala Glu Ser Ile Val Thr Gly

65 70 75 80

Ala Lys Ala Glu Ala Glu Arg Leu Ala Ala Glu Ala Lys Ala Lys Ile

85 90 95

Glu Asp Phe Val Thr Arg Arg Thr Lys Met Ala Glu Asp Lys Ile Ala

100 105 110

Gln Ala Glu His Gln Ala Leu Ala Asp Val Lys Ser Ile Ala Ala Glu

115 120 125

Ala Ala Ala Lys Ala Ala Glu Val Ile Leu Gly Ala Gln Ala Thr Gly

130 135 140

Ala Val Ala Glu Arg Leu Leu Ser Gly Ala Ile Ser Glu Val Lys Thr

145 150 155 160

Lys Leu Asn

<210> 42

<211> 729

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 42

atgatgattg catggaagcg gaccttcgca gtcgtgacct tcggggccgc cctgatggcc 60

atgcccgtcg cgggcgtggt cgcagctgag acttctcccg ctccggcggc agtggcgcag 120

gccgatcatg cggtgcccac cgaggcggcc ggccagggca ccgccgatgc ggcccatgcc 180

gccgcgccgg gcgaggccgc ccatggtggc gcggccaagc acgaaaccca tttcccgccc 240

ttcgacggca ccaccttcgc ctcccagttg ctgtggctcg ccgtcacctt cggcctgctt 300

tactacctca tgagcaaggt cacgctgccg cgcatcggcc gcatcctgga agagcgccac 360

gaccgcatcg ccgatgatct ggaggaagcc tccaagcatc gcgccgagag cgaggccgcc 420

cagcgggcct atgagaaggc gctgagcgag gcccgcgcga aggcccattc catcgccgcg 480

gaaacccgcg accgccttgc cgcccacgcc gacaccaacc gcaaggcgct ggagagcgag 540

ctcaccgcca agctgcaggc ggccgaggag cgcatcgcca ccaccaagag cgaagccctc 600

acccatgtgc gcggcatcgc ggtggacgcc acccaatcca tcgtctccac cctcatcggt 660

gtcgcgcccg cggcggccga cgtggaaaaa gcggtggacg gcgccctgtc ccagcacggc 720

caggcctga 729

<210> 43

<211> 242

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 43

Met Met Ile Ala Trp Lys Arg Thr Phe Ala Val Val Thr Phe Gly Ala

1 5 10 15

Ala Leu Met Ala Met Pro Val Ala Gly Val Val Ala Ala Glu Thr Ser

20 25 30

Pro Ala Pro Ala Ala Val Ala Gln Ala Asp His Ala Val Pro Thr Glu

35 40 45

Ala Ala Gly Gln Gly Thr Ala Asp Ala Ala His Ala Ala Ala Pro Gly

50 55 60

Glu Ala Ala His Gly Gly Ala Ala Lys His Glu Thr His Phe Pro Pro

65 70 75 80

Phe Asp Gly Thr Thr Phe Ala Ser Gln Leu Leu Trp Leu Ala Val Thr

85 90 95

Phe Gly Leu Leu Tyr Tyr Leu Met Ser Lys Val Thr Leu Pro Arg Ile

100 105 110

Gly Arg Ile Leu Glu Glu Arg His Asp Arg Ile Ala Asp Asp Leu Glu

115 120 125

Glu Ala Ser Lys His Arg Ala Glu Ser Glu Ala Ala Gln Arg Ala Tyr

130 135 140

Glu Lys Ala Leu Ser Glu Ala Arg Ala Lys Ala His Ser Ile Ala Ala

145 150 155 160

Glu Thr Arg Asp Arg Leu Ala Ala His Ala Asp Thr Asn Arg Lys Ala

165 170 175

Leu Glu Ser Glu Leu Thr Ala Lys Leu Gln Ala Ala Glu Glu Arg Ile

180 185 190

Ala Thr Thr Lys Ser Glu Ala Leu Thr His Val Arg Gly Ile Ala Val

195 200 205

Asp Ala Thr Gln Ser Ile Val Ser Thr Leu Ile Gly Val Ala Pro Ala

210 215 220

Ala Ala Asp Val Glu Lys Ala Val Asp Gly Ala Leu Ser Gln His Gly

225 230 235 240

Gln Ala

<210> 44

<211> 228

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 44

atggaagcgg aagctggaaa gttcatcggt gccggcctcg cctgcctcgg catgggtctc 60

gctggcgtcg gcgtcggtaa catcttcggt aacttcctct ccggcgccct gcgcaacccg 120

tccgctgccg acggccagtt cgcccgcgcc ttcatcggcg ccgccctcgc ggaaggtctc 180

ggcatcttct cgctggtcgt tgcgctcgtc ctgctgttcg tggcctga 228

<210> 45

<211> 75

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 45

Met Glu Ala Glu Ala Gly Lys Phe Ile Gly Ala Gly Leu Ala Cys Leu

1 5 10 15

Gly Met Gly Leu Ala Gly Val Gly Val Gly Asn Ile Phe Gly Asn Phe

20 25 30

Leu Ser Gly Ala Leu Arg Asn Pro Ser Ala Ala Asp Gly Gln Phe Ala

35 40 45

Arg Ala Phe Ile Gly Ala Ala Leu Ala Glu Gly Leu Gly Ile Phe Ser

50 55 60

Leu Val Val Ala Leu Val Leu Leu Phe Val Ala

65 70 75

<210> 46

<211> 753

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 46

atgaccgtcg atccgatcca ccagttcgag atcaagcgct acgtggatct gctgaacgtc 60

ggcggtgtcc agttctcctt caccaacgca acggtgttca tgattggcat cgtcctggtg 120

attttcttct tcctgacttt cgcgacacgc ggtcgcaccc ttgtgccggg ccggatgcag 180

tcggcggcgg agctgagcta cgagttcatc gccaagatgg tgcgcgacgc ggccggcagc 240

gagggaatgg tgttctttcc cttcgtcttc tcgctcttca tgttcgtgct ggtggcgaac 300

gtattggggc tcatccccta caccttcacg gtgaccgccc acctcatcgt caccgccgcc 360

ctggcggcga cggtgatcct caccgtcatc atctacggct tcgtgcggca cggcacccac 420

ttcctgcacc tgttcgtgcc gtcgggcgtg ccgggcttcc tcctgccctt cctcgtggtg 480

atcgaggtgg tgtcgttcct gtcgcggccc atcagcctct cgctgcgtct gttcgccaac 540

atgctggcgg gccacatcgc cctcaaggtg ttcgccttct tcgtcgtggg actggcctcg 600

gccggcgcga tcggctggtt cggcgccacc ctgcccttct tcatgatcgt ggcgctcacc 660

gcgctggagc tgctggtggc ggtgctgcag gcctacgtgt tcgcggtgct gacctcgatc 720

tacctcaacg acgccatcca tcccggccac tga 753

<210> 47

<211> 250

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 47

Met Thr Val Asp Pro Ile His Gln Phe Glu Ile Lys Arg Tyr Val Asp

1 5 10 15

Leu Leu Asn Val Gly Gly Val Gln Phe Ser Phe Thr Asn Ala Thr Val

20 25 30

Phe Met Ile Gly Ile Val Leu Val Ile Phe Phe Phe Leu Thr Phe Ala

35 40 45

Thr Arg Gly Arg Thr Leu Val Pro Gly Arg Met Gln Ser Ala Ala Glu

50 55 60

Leu Ser Tyr Glu Phe Ile Ala Lys Met Val Arg Asp Ala Ala Gly Ser

65 70 75 80

Glu Gly Met Val Phe Phe Pro Phe Val Phe Ser Leu Phe Met Phe Val

85 90 95

Leu Val Ala Asn Val Leu Gly Leu Ile Pro Tyr Thr Phe Thr Val Thr

100 105 110

Ala His Leu Ile Val Thr Ala Ala Leu Ala Ala Thr Val Ile Leu Thr

115 120 125

Val Ile Ile Tyr Gly Phe Val Arg His Gly Thr His Phe Leu His Leu

130 135 140

Phe Val Pro Ser Gly Val Pro Gly Phe Leu Leu Pro Phe Leu Val Val

145 150 155 160

Ile Glu Val Val Ser Phe Leu Ser Arg Pro Ile Ser Leu Ser Leu Arg

165 170 175

Leu Phe Ala Asn Met Leu Ala Gly His Ile Ala Leu Lys Val Phe Ala

180 185 190

Phe Phe Val Val Gly Leu Ala Ser Ala Gly Ala Ile Gly Trp Phe Gly

195 200 205

Ala Thr Leu Pro Phe Phe Met Ile Val Ala Leu Thr Ala Leu Glu Leu

210 215 220

Leu Val Ala Val Leu Gln Ala Tyr Val Phe Ala Val Leu Thr Ser Ile

225 230 235 240

Tyr Leu Asn Asp Ala Ile His Pro Gly His

245 250

<210> 48

<211> 390

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 48

atgtccgagc cgaatgatcc atcccgcagg gacggtgcga aggcgaaaga cgagacgcag 60

gactcccggc ccggtgaggc ggatcttgct cggcgcctcg atgcgctcgg cacctccatc 120

ggtcaggtca agtccagaag cggggagccc gcggcgacgc cgcgcaagga cacctcctcg 180

gcctccggcg cggccctggc gtttcggctg ggcgccgagt ttgtttcagg cgtgctggtg 240

ggctcgctca tcggctacgg gttggattat gcgtttgcga tttcgccctg ggggctgatc 300

gccttcacgc tgatcggctt tgccgccggc gtcctgaaca tgctgcgcgt ggcgaacagc 360

gatgccaagc gccacagcgc ggacaggtga 390

<210> 49

<211> 129

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 49

Met Ser Glu Pro Asn Asp Pro Ser Arg Arg Asp Gly Ala Lys Ala Lys

1 5 10 15

Asp Glu Thr Gln Asp Ser Arg Pro Gly Glu Ala Asp Leu Ala Arg Arg

20 25 30

Leu Asp Ala Leu Gly Thr Ser Ile Gly Gln Val Lys Ser Arg Ser Gly

35 40 45

Glu Pro Ala Ala Thr Pro Arg Lys Asp Thr Ser Ser Ala Ser Gly Ala

50 55 60

Ala Leu Ala Phe Arg Leu Gly Ala Glu Phe Val Ser Gly Val Leu Val

65 70 75 80

Gly Ser Leu Ile Gly Tyr Gly Leu Asp Tyr Ala Phe Ala Ile Ser Pro

85 90 95

Trp Gly Leu Ile Ala Phe Thr Leu Ile Gly Phe Ala Ala Gly Val Leu

100 105 110

Asn Met Leu Arg Val Ala Asn Ser Asp Ala Lys Arg His Ser Ala Asp

115 120 125

Arg

<210> 50

<211> 1929

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 50

atgagttcgc tctccgccac tattcaacag gtcttcaacg agccgggctg cgcgaagaac 60

cagaataagt ccgaggcgga gaagaagaag ggctgcacca agcagctgca acccggcgga 120

gcggccggcg gctgcgcgtt cgacggcgcg aagatcgcgc tccagccctt gaccgacgtc 180

gcccacctgg tgcacggccc catcgcctgc gaaggcaatt cctgggacaa tcgtggcgcc 240

aagtcctccg gctcgaacat ctggcgcacc ggcttcacca cggacatcaa cgaaaccgac 300

gtggtgttcg gcggcgagaa gcgtctgttc aagtccatca aggaaatcat cgagaagtac 360

gacccgccgg ccgtcttcgt ctatcagacc tgcgtccccg ccatgatcgg cgacgacatc 420

gacgcggtgt gcaaggcggc cagggagaag ttcggaaagc cggtgatccc gatcaattcc 480

cccggcttcg tggggccgaa gaatctcggc aacaagctcg ccggcgaggc gctcctcgac 540

catgtgatcg gcaccgagga gcccgattac acgacggcct acgacatcaa catcatcggc 600

gaatacaatc tctccggcga gttgtggcag gtgaagccgc tgctggacga gctgggcatc 660

cgcatcctcg cctgcatctc cggcgacggg aagtacaagg atgtggcgtc ctcccaccgc 720

gccaaggcgg cgatgatggt gtgctccaag gccatgatca acgtggcccg caagatggag 780

gagcgctacg acatcccctt cttcgaaggc tccttctacg gcatcgagga tagctccgat 840

tccctgcgcg agattgcgcg catgctcatc gagaagggcg ccgatccgga gctgatggac 900

cgcaccgagg cgctgattga gcgggaagag aagaaggcgt gggacgccat cgccgcctac 960

aagccccgct tcaaggacaa gaaggtgctg ctcatcaccg gcggcgtgaa atcctggtcg 1020

gtggtggcag cgctccagga agccggcctc gaactggtgg gcacctcggt gaagaagtcc 1080

accaaggagg acaaggagcg catcaaggaa ctgatgggcc aggacgccca catgatcgac 1140

gacatgacgc cccgcgaaat gtacaagatg ctgaaggacg ccaaggcgga catcatgctc 1200

tcgggcgggc gctcgcaatt catcgcgctc aaggccgcca tgccctggct cgacatcaac 1260

caggagcgcc accacgccta tatgggctat gtgggcatgg tgaagctggt cgaggagatc 1320

gacaaggcgc tctacaatcc cgtgtgggaa caggtgcgca agcccgcccc gtgggaaaat 1380

ccggaagaca cctggcaggc ccgtgcgctc gccgaaatgg aggcggaggc cgccgcgctc 1440

gccgccgatc cggtgcgcgc ggaagaggtg cgccggtcca agaagatctg caattgcaag 1500

agcgtcgacc tcggaaccat tgaggacgcc atcaaggctc acgcgctgac caccgtggag 1560

ggtgtgcgag agcacaccaa tgcctcggga ggctgcggag cctgcagcgg gcggatcgag 1620

gagatcttcg aggccgtggg cgttgtcgcc gccccgcctc ccgcggaggc cgccccgtct 1680

ccgcaggaga tcgcgcccga tccgctcgct gcggaggaaa agcgccgcgc caagaaggcc 1740

tgcggctgca aggaggtagc ggtcggcacc attgaggatg ccatccgcgc caagggtctg 1800

cgaaacatcg cggaggtgcg tgcggccacc gatgccaaca ccggctgcgg caattgccag 1860

gagcgggtgg agggcatcct cgaccgggtt ctcgccgagg cggcctcaga actccaggcg 1920

gcggaatag 1929

<210> 51

<211> 642

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 51

Met Ser Ser Leu Ser Ala Thr Ile Gln Gln Val Phe Asn Glu Pro Gly

1 5 10 15

Cys Ala Lys Asn Gln Asn Lys Ser Glu Ala Glu Lys Lys Lys Gly Cys

20 25 30

Thr Lys Gln Leu Gln Pro Gly Gly Ala Ala Gly Gly Cys Ala Phe Asp

35 40 45

Gly Ala Lys Ile Ala Leu Gln Pro Leu Thr Asp Val Ala His Leu Val

50 55 60

His Gly Pro Ile Ala Cys Glu Gly Asn Ser Trp Asp Asn Arg Gly Ala

65 70 75 80

Lys Ser Ser Gly Ser Asn Ile Trp Arg Thr Gly Phe Thr Thr Asp Ile

85 90 95

Asn Glu Thr Asp Val Val Phe Gly Gly Glu Lys Arg Leu Phe Lys Ser

100 105 110

Ile Lys Glu Ile Ile Glu Lys Tyr Asp Pro Pro Ala Val Phe Val Tyr

115 120 125

Gln Thr Cys Val Pro Ala Met Ile Gly Asp Asp Ile Asp Ala Val Cys

130 135 140

Lys Ala Ala Arg Glu Lys Phe Gly Lys Pro Val Ile Pro Ile Asn Ser

145 150 155 160

Pro Gly Phe Val Gly Pro Lys Asn Leu Gly Asn Lys Leu Ala Gly Glu

165 170 175

Ala Leu Leu Asp His Val Ile Gly Thr Glu Glu Pro Asp Tyr Thr Thr

180 185 190

Ala Tyr Asp Ile Asn Ile Ile Gly Glu Tyr Asn Leu Ser Gly Glu Leu

195 200 205

Trp Gln Val Lys Pro Leu Leu Asp Glu Leu Gly Ile Arg Ile Leu Ala

210 215 220

Cys Ile Ser Gly Asp Gly Lys Tyr Lys Asp Val Ala Ser Ser His Arg

225 230 235 240

Ala Lys Ala Ala Met Met Val Cys Ser Lys Ala Met Ile Asn Val Ala

245 250 255

Arg Lys Met Glu Glu Arg Tyr Asp Ile Pro Phe Phe Glu Gly Ser Phe

260 265 270

Tyr Gly Ile Glu Asp Ser Ser Asp Ser Leu Arg Glu Ile Ala Arg Met

275 280 285

Leu Ile Glu Lys Gly Ala Asp Pro Glu Leu Met Asp Arg Thr Glu Ala

290 295 300

Leu Ile Glu Arg Glu Glu Lys Lys Ala Trp Asp Ala Ile Ala Ala Tyr

305 310 315 320

Lys Pro Arg Phe Lys Asp Lys Lys Val Leu Leu Ile Thr Gly Gly Val

325 330 335

Lys Ser Trp Ser Val Val Ala Ala Leu Gln Glu Ala Gly Leu Glu Leu

340 345 350

Val Gly Thr Ser Val Lys Lys Ser Thr Lys Glu Asp Lys Glu Arg Ile

355 360 365

Lys Glu Leu Met Gly Gln Asp Ala His Met Ile Asp Asp Met Thr Pro

370 375 380

Arg Glu Met Tyr Lys Met Leu Lys Asp Ala Lys Ala Asp Ile Met Leu

385 390 395 400

Ser Gly Gly Arg Ser Gln Phe Ile Ala Leu Lys Ala Ala Met Pro Trp

405 410 415

Leu Asp Ile Asn Gln Glu Arg His His Ala Tyr Met Gly Tyr Val Gly

420 425 430

Met Val Lys Leu Val Glu Glu Ile Asp Lys Ala Leu Tyr Asn Pro Val

435 440 445

Trp Glu Gln Val Arg Lys Pro Ala Pro Trp Glu Asn Pro Glu Asp Thr

450 455 460

Trp Gln Ala Arg Ala Leu Ala Glu Met Glu Ala Glu Ala Ala Ala Leu

465 470 475 480

Ala Ala Asp Pro Val Arg Ala Glu Glu Val Arg Arg Ser Lys Lys Ile

485 490 495

Cys Asn Cys Lys Ser Val Asp Leu Gly Thr Ile Glu Asp Ala Ile Lys

500 505 510

Ala His Ala Leu Thr Thr Val Glu Gly Val Arg Glu His Thr Asn Ala

515 520 525

Ser Gly Gly Cys Gly Ala Cys Ser Gly Arg Ile Glu Glu Ile Phe Glu

530 535 540

Ala Val Gly Val Val Ala Ala Pro Pro Pro Ala Glu Ala Ala Pro Ser

545 550 555 560

Pro Gln Glu Ile Ala Pro Asp Pro Leu Ala Ala Glu Glu Lys Arg Arg

565 570 575

Ala Lys Lys Ala Cys Gly Cys Lys Glu Val Ala Val Gly Thr Ile Glu

580 585 590

Asp Ala Ile Arg Ala Lys Gly Leu Arg Asn Ile Ala Glu Val Arg Ala

595 600 605

Ala Thr Asp Ala Asn Thr Gly Cys Gly Asn Cys Gln Glu Arg Val Glu

610 615 620

Gly Ile Leu Asp Arg Val Leu Ala Glu Ala Ala Ser Glu Leu Gln Ala

625 630 635 640

Ala Glu

<210> 52

<211> 1503

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 52

atgagtgtcg cacagtccca gagcgtcgcc gagatcaagg cgcgcaacaa ggaactcatc 60

gaagaggtcc tcaaggtcta tcccgagaag accgccaagc gccgcgccaa gcacctgaac 120

gtccacgaag ccggcaagtc cgactgcggc gtgaagtcca acatcaagtc catcccgggc 180

gtgatgacca tccgcggttg cgcttatgcc ggctccaagg gtgtggtgtg gggtcccatc 240

aaggacatga tccacatctc ccacggcccg gtgggctgcg gccagtatag ctgggccgcc 300

cgccgcaact actatatcgg cacgaccggc atcgacacct tcgtgacgat gcagttcacc 360

tccgacttcc aggagaagga catcgtcttc ggcggcgaca agaagctcgc caagatcatg 420

gacgagatcc aggagctgtt cccgctgaac aacggcatca ccgttcagtc cgagtgcccc 480

atcggcctca tcggcgacga catcgaggcc gtctccaagc agaagtccaa ggagtatgag 540

ggcaagacca tcgtgccggt gcgctgcgag ggcttccgcg gcgtgtccca gtccctgggc 600

caccacatcg ccaacgacgc catccgcgat tgggtgttcg acaagatcgc gcccgacgcc 660

gagccgcgct ttgagccgac cccgtacgac gtcgccatca tcggcgacta caatatcggt 720

ggtgacgcct ggtcgtcccg tatcctcctg gaggagatgg gcctgcgcgt gatcgcccag 780

tggtccggcg acggttcgct cgctgagctg gaggccaccc cgaaggccaa gctcaacgtg 840

ctgcactgct accgctccat gaactacatc tcgcgccaca tggaagagaa gtacggtatc 900

ccgtggtgcg agtacaactt cttcggtcct tccaagatcg ccgagtccct gcgcaagatc 960

gccagctact tcgacgacaa gatcaaggaa ggcgcggagc gcgtcatcgc caagtatcag 1020

ccgctcatgg atgcggtgat cgcgaagtat cgtccccgcc tcgagggcaa gaccgtgatg 1080

ctgtacgtgg gcggcctgcg tccccgtcac gtcatcggcg cctacgagga cctgggcatg 1140

gaagtggtcg gcacgggcta cgagttcgcc cataacgacg actaccagcg caccgcccag 1200

cactacgtca aggatggcac catcatctat gacgacgtga ccggctacga gttcgagaag 1260

ttcgtcgaga agatccagcc ggacctggtc ggttcgggca tcaaggaaaa gtacgtcttc 1320

cagaagatgg gcgtgccgtt ccgccagatg cactcctggg actactcggg cccgtaccac 1380

ggctatgacg gcttcgcgat cttcgcgcgc gacatggaca tggccatcaa cagccccgtg 1440

tggaagatga cccaggctcc gtggaagagc gtccccaagc cgacgatgct cgcggctgaa 1500

tga 1503

<210> 53

<211> 500

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 53

Met Ser Val Ala Gln Ser Gln Ser Val Ala Glu Ile Lys Ala Arg Asn

1 5 10 15

Lys Glu Leu Ile Glu Glu Val Leu Lys Val Tyr Pro Glu Lys Thr Ala

20 25 30

Lys Arg Arg Ala Lys His Leu Asn Val His Glu Ala Gly Lys Ser Asp

35 40 45

Cys Gly Val Lys Ser Asn Ile Lys Ser Ile Pro Gly Val Met Thr Ile

50 55 60

Arg Gly Cys Ala Tyr Ala Gly Ser Lys Gly Val Val Trp Gly Pro Ile

65 70 75 80

Lys Asp Met Ile His Ile Ser His Gly Pro Val Gly Cys Gly Gln Tyr

85 90 95

Ser Trp Ala Ala Arg Arg Asn Tyr Tyr Ile Gly Thr Thr Gly Ile Asp

100 105 110

Thr Phe Val Thr Met Gln Phe Thr Ser Asp Phe Gln Glu Lys Asp Ile

115 120 125

Val Phe Gly Gly Asp Lys Lys Leu Ala Lys Ile Met Asp Glu Ile Gln

130 135 140

Glu Leu Phe Pro Leu Asn Asn Gly Ile Thr Val Gln Ser Glu Cys Pro

145 150 155 160

Ile Gly Leu Ile Gly Asp Asp Ile Glu Ala Val Ser Lys Gln Lys Ser

165 170 175

Lys Glu Tyr Glu Gly Lys Thr Ile Val Pro Val Arg Cys Glu Gly Phe

180 185 190

Arg Gly Val Ser Gln Ser Leu Gly His His Ile Ala Asn Asp Ala Ile

195 200 205

Arg Asp Trp Val Phe Asp Lys Ile Ala Pro Asp Ala Glu Pro Arg Phe

210 215 220

Glu Pro Thr Pro Tyr Asp Val Ala Ile Ile Gly Asp Tyr Asn Ile Gly

225 230 235 240

Gly Asp Ala Trp Ser Ser Arg Ile Leu Leu Glu Glu Met Gly Leu Arg

245 250 255

Val Ile Ala Gln Trp Ser Gly Asp Gly Ser Leu Ala Glu Leu Glu Ala

260 265 270

Thr Pro Lys Ala Lys Leu Asn Val Leu His Cys Tyr Arg Ser Met Asn

275 280 285

Tyr Ile Ser Arg His Met Glu Glu Lys Tyr Gly Ile Pro Trp Cys Glu

290 295 300

Tyr Asn Phe Phe Gly Pro Ser Lys Ile Ala Glu Ser Leu Arg Lys Ile

305 310 315 320

Ala Ser Tyr Phe Asp Asp Lys Ile Lys Glu Gly Ala Glu Arg Val Ile

325 330 335

Ala Lys Tyr Gln Pro Leu Met Asp Ala Val Ile Ala Lys Tyr Arg Pro

340 345 350

Arg Leu Glu Gly Lys Thr Val Met Leu Tyr Val Gly Gly Leu Arg Pro

355 360 365

Arg His Val Ile Gly Ala Tyr Glu Asp Leu Gly Met Glu Val Val Gly

370 375 380

Thr Gly Tyr Glu Phe Ala His Asn Asp Asp Tyr Gln Arg Thr Ala Gln

385 390 395 400

His Tyr Val Lys Asp Gly Thr Ile Ile Tyr Asp Asp Val Thr Gly Tyr

405 410 415

Glu Phe Glu Lys Phe Val Glu Lys Ile Gln Pro Asp Leu Val Gly Ser

420 425 430

Gly Ile Lys Glu Lys Tyr Val Phe Gln Lys Met Gly Val Pro Phe Arg

435 440 445

Gln Met His Ser Trp Asp Tyr Ser Gly Pro Tyr His Gly Tyr Asp Gly

450 455 460

Phe Ala Ile Phe Ala Arg Asp Met Asp Met Ala Ile Asn Ser Pro Val

465 470 475 480

Trp Lys Met Thr Gln Ala Pro Trp Lys Ser Val Pro Lys Pro Thr Met

485 490 495

Leu Ala Ala Glu

500

<210> 54

<211> 1377

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 54

atggccaccg tttccgtctc caagaaggcc tgcgcggtca accccctcaa gatgagccag 60

ccggtgggcg gcgcgctcgc cttcatgggc gtgcgcaagg ccatgccgct gctgcacggc 120

tcgcagggct gcacctcctt cggcctggtg ctgttcgtgc gccacttcaa ggaagccatc 180

cccatgcaga ccaccgccat gagcgaggtg gcgacggttc tgggcggcct tgagaatgtg 240

gagcaggcca ttctcaacat ctacaatcgc accaagccgg agatcatcgg catctgctcc 300

accggcgtca ccgagaccaa gggcgatgat gtcgacggct acatcaagct gatccgggac 360

aagtatcccc agctggccga cttcccgctg gtctatgtct ccacccccga tttcaaggac 420

gccttccagg acggttggga gaagaccgtg gcgaagatgg tggaggcgct ggtgaagccc 480

gccgccgaca agcagaagga caagacccgc gtcaacgtcc tgcccggctg ccacctcacg 540

cccggcgatc tggatgagat gcggaccatc ttcgaggatt tcgggctcac accctatttc 600

ctgccggatc tggccggctc gctggatggg catatccccg aggacttctc gcccaccacc 660

atcggcggca tcggcatcga tgagatcgcc accatgggcg aggcggccca caccatctgc 720

atcggcgcgc agatgcgccg ggcgggcgag gccatggaga agaagaccgg cattcccttc 780

aagctgttcg agcgcctgtg cggcctggag gcgaacgacg ccttcatcat gcacctgtcg 840

cagatctccg gccggccggt gccggtgaag tatcgccggc agcggggcca gctggtggat 900

gccatgctgg acggccactt ccatctgggc ggtcgcaagg tggccatggg ggcggagccg 960

gacctgctct acgacgtggg ctccttcctg cacgagatgg gcgcccacat cctttccgcg 1020

gtcaccacca cccagtcgcc ggtgctggcg cgcctgcctg ccgaggaggt gcttatcggc 1080

gacctggagg atctggagac ccaggcgaag gcgcgcggat gcgatctcct gctcacccat 1140

tcccatgggc gccaggcggc ggagcgcctc cacatcccct tctaccggat cggcattccc 1200

atgtttgacc ggctgggggc ggggcatctg ttgtcggtgg gctatcgcgg cacccgcgac 1260

ctcatcttcc atctcgccaa ccttgtgatc gccgaccacg aggaaaatca cgagccgacg 1320

cccgacacct gggccaccgg ccatggcgag catgccgccg cccccacttc ccattga 1377

<210> 55

<211> 458

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 55

Met Ala Thr Val Ser Val Ser Lys Lys Ala Cys Ala Val Asn Pro Leu

1 5 10 15

Lys Met Ser Gln Pro Val Gly Gly Ala Leu Ala Phe Met Gly Val Arg

20 25 30

Lys Ala Met Pro Leu Leu His Gly Ser Gln Gly Cys Thr Ser Phe Gly

35 40 45

Leu Val Leu Phe Val Arg His Phe Lys Glu Ala Ile Pro Met Gln Thr

50 55 60

Thr Ala Met Ser Glu Val Ala Thr Val Leu Gly Gly Leu Glu Asn Val

65 70 75 80

Glu Gln Ala Ile Leu Asn Ile Tyr Asn Arg Thr Lys Pro Glu Ile Ile

85 90 95

Gly Ile Cys Ser Thr Gly Val Thr Glu Thr Lys Gly Asp Asp Val Asp

100 105 110

Gly Tyr Ile Lys Leu Ile Arg Asp Lys Tyr Pro Gln Leu Ala Asp Phe

115 120 125

Pro Leu Val Tyr Val Ser Thr Pro Asp Phe Lys Asp Ala Phe Gln Asp

130 135 140

Gly Trp Glu Lys Thr Val Ala Lys Met Val Glu Ala Leu Val Lys Pro

145 150 155 160

Ala Ala Asp Lys Gln Lys Asp Lys Thr Arg Val Asn Val Leu Pro Gly

165 170 175

Cys His Leu Thr Pro Gly Asp Leu Asp Glu Met Arg Thr Ile Phe Glu

180 185 190

Asp Phe Gly Leu Thr Pro Tyr Phe Leu Pro Asp Leu Ala Gly Ser Leu

195 200 205

Asp Gly His Ile Pro Glu Asp Phe Ser Pro Thr Thr Ile Gly Gly Ile

210 215 220

Gly Ile Asp Glu Ile Ala Thr Met Gly Glu Ala Ala His Thr Ile Cys

225 230 235 240

Ile Gly Ala Gln Met Arg Arg Ala Gly Glu Ala Met Glu Lys Lys Thr

245 250 255

Gly Ile Pro Phe Lys Leu Phe Glu Arg Leu Cys Gly Leu Glu Ala Asn

260 265 270

Asp Ala Phe Ile Met His Leu Ser Gln Ile Ser Gly Arg Pro Val Pro

275 280 285

Val Lys Tyr Arg Arg Gln Arg Gly Gln Leu Val Asp Ala Met Leu Asp

290 295 300

Gly His Phe His Leu Gly Gly Arg Lys Val Ala Met Gly Ala Glu Pro

305 310 315 320

Asp Leu Leu Tyr Asp Val Gly Ser Phe Leu His Glu Met Gly Ala His

325 330 335

Ile Leu Ser Ala Val Thr Thr Thr Gln Ser Pro Val Leu Ala Arg Leu

340 345 350

Pro Ala Glu Glu Val Leu Ile Gly Asp Leu Glu Asp Leu Glu Thr Gln

355 360 365

Ala Lys Ala Arg Gly Cys Asp Leu Leu Leu Thr His Ser His Gly Arg

370 375 380

Gln Ala Ala Glu Arg Leu His Ile Pro Phe Tyr Arg Ile Gly Ile Pro

385 390 395 400

Met Phe Asp Arg Leu Gly Ala Gly His Leu Leu Ser Val Gly Tyr Arg

405 410 415

Gly Thr Arg Asp Leu Ile Phe His Leu Ala Asn Leu Val Ile Ala Asp

420 425 430

His Glu Glu Asn His Glu Pro Thr Pro Asp Thr Trp Ala Thr Gly His

435 440 445

Gly Glu His Ala Ala Ala Pro Thr Ser His

450 455

<210> 56

<211> 1560

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 56

atgccacaaa atgctgacaa tgtgctcgat cacttcgagc tcttccgtgg tcccgaatac 60

cagcagatgc tggccaataa gaaaaagatg ttcgagaacc cccgcgatcc ggccgaagtc 120

gagcgcgtgc gggaatgggc gaagactcct gaatacaagg agctgaactt cgcccgcgag 180

gcgctcaccg tgaatccggc caaggcttgt cagccgctgg gcgcggtgtt cgtcgccgtc 240

ggcttcgaga gcacgatccc cttcgtgcac ggctcgcagg gttgcgtcgc gtattaccgc 300

tcgcacctct cccgccactt caaggagccg tcctcctgcg tctcctcgtc catgaccgag 360

gatgcggcgg tgttcggcgg cctcaacaac atgattgacg gcctcgccaa cacctacaac 420

atgtacaagc cgaagatgat cgccgtctcc accacctgca tggcggaagt catcggcgac 480

gatctgaacg ccttcatcaa gaccgcgaag gaaaagggct cggttccggc cgaatacgac 540

gtgcccttcg cccacacccc ggcgttcgtc ggcagccatg tcaccggcta cgacaatgcg 600

ctcaagggca tcctcgagca cttctgggac ggcaaggccg gcaccgcgcc gaagctggag 660

cgcgttccca acgagaagat caacttcatc ggcggcttcg acggctacac cgtcggcaac 720

actcgcgaag tgaagcgcat cttcgaggcg ttcggcgccg attacaccat cctcgccgac 780

aattccgaag tgttcgacac cccgaccgac ggcgagttcc gcatgtatga cggcggcacg 840

accctggagg acgcggcgaa cgcggtgcac gccaaggcca ccatctccat gcaggaatac 900

tgcacggaga agaccctgcc catgatcgcc ggtcatggcc aggacgtggt cgccctcaac 960

caccccgtgg gcgtgggcgg caccgacaag ttcctcatgg agatcgcccg cctcaccggc 1020

aaggagatcc ccgaggagct gacccgcgag cgcggccgtc tcgtggacgc tatcgcggac 1080

tcttccgcgc acatccacgg caagaagttc gccatctacg gcgatccgga tctgtgcctg 1140

ggcctcgccg cgttcctgct ggagctgggc gccgagccga cccatgtgct ggccaccaac 1200

ggcaccaaga agtgggccga gaaggttcag gaactgttcg actcttcgcc gttcggcgcc 1260

aactgcaagg tctatcccgg caaggacctg tggcacatgc gctcgctcct gttcgtggag 1320

ccggtggatt tcatcatcgg caacacctac ggcaagtatc tcgagcgcga cacgggcacc 1380

ccgctgatcc gtatcggctt cccggtgttc gaccgtcacc accaccaccg ccgtccggtg 1440

tggggctatc agggcggcat gaacgtcctg atcacgatcc tcgacaagat ctttgacgag 1500

atcgaccgca acaccaacgt gccggccaag accgactact cgttcgacat cattcgttga 1560

<210> 57

<211> 519

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 57

Met Pro Gln Asn Ala Asp Asn Val Leu Asp His Phe Glu Leu Phe Arg

1 5 10 15

Gly Pro Glu Tyr Gln Gln Met Leu Ala Asn Lys Lys Lys Met Phe Glu

20 25 30

Asn Pro Arg Asp Pro Ala Glu Val Glu Arg Val Arg Glu Trp Ala Lys

35 40 45

Thr Pro Glu Tyr Lys Glu Leu Asn Phe Ala Arg Glu Ala Leu Thr Val

50 55 60

Asn Pro Ala Lys Ala Cys Gln Pro Leu Gly Ala Val Phe Val Ala Val

65 70 75 80

Gly Phe Glu Ser Thr Ile Pro Phe Val His Gly Ser Gln Gly Cys Val

85 90 95

Ala Tyr Tyr Arg Ser His Leu Ser Arg His Phe Lys Glu Pro Ser Ser

100 105 110

Cys Val Ser Ser Ser Met Thr Glu Asp Ala Ala Val Phe Gly Gly Leu

115 120 125

Asn Asn Met Ile Asp Gly Leu Ala Asn Thr Tyr Asn Met Tyr Lys Pro

130 135 140

Lys Met Ile Ala Val Ser Thr Thr Cys Met Ala Glu Val Ile Gly Asp

145 150 155 160

Asp Leu Asn Ala Phe Ile Lys Thr Ala Lys Glu Lys Gly Ser Val Pro

165 170 175

Ala Glu Tyr Asp Val Pro Phe Ala His Thr Pro Ala Phe Val Gly Ser

180 185 190

His Val Thr Gly Tyr Asp Asn Ala Leu Lys Gly Ile Leu Glu His Phe

195 200 205

Trp Asp Gly Lys Ala Gly Thr Ala Pro Lys Leu Glu Arg Val Pro Asn

210 215 220

Glu Lys Ile Asn Phe Ile Gly Gly Phe Asp Gly Tyr Thr Val Gly Asn

225 230 235 240

Thr Arg Glu Val Lys Arg Ile Phe Glu Ala Phe Gly Ala Asp Tyr Thr

245 250 255

Ile Leu Ala Asp Asn Ser Glu Val Phe Asp Thr Pro Thr Asp Gly Glu

260 265 270

Phe Arg Met Tyr Asp Gly Gly Thr Thr Leu Glu Asp Ala Ala Asn Ala

275 280 285

Val His Ala Lys Ala Thr Ile Ser Met Gln Glu Tyr Cys Thr Glu Lys

290 295 300

Thr Leu Pro Met Ile Ala Gly His Gly Gln Asp Val Val Ala Leu Asn

305 310 315 320

His Pro Val Gly Val Gly Gly Thr Asp Lys Phe Leu Met Glu Ile Ala

325 330 335

Arg Leu Thr Gly Lys Glu Ile Pro Glu Glu Leu Thr Arg Glu Arg Gly

340 345 350

Arg Leu Val Asp Ala Ile Ala Asp Ser Ser Ala His Ile His Gly Lys

355 360 365

Lys Phe Ala Ile Tyr Gly Asp Pro Asp Leu Cys Leu Gly Leu Ala Ala

370 375 380

Phe Leu Leu Glu Leu Gly Ala Glu Pro Thr His Val Leu Ala Thr Asn

385 390 395 400

Gly Thr Lys Lys Trp Ala Glu Lys Val Gln Glu Leu Phe Asp Ser Ser

405 410 415

Pro Phe Gly Ala Asn Cys Lys Val Tyr Pro Gly Lys Asp Leu Trp His

420 425 430

Met Arg Ser Leu Leu Phe Val Glu Pro Val Asp Phe Ile Ile Gly Asn

435 440 445

Thr Tyr Gly Lys Tyr Leu Glu Arg Asp Thr Gly Thr Pro Leu Ile Arg

450 455 460

Ile Gly Phe Pro Val Phe Asp Arg His His His His Arg Arg Pro Val

465 470 475 480

Trp Gly Tyr Gln Gly Gly Met Asn Val Leu Ile Thr Ile Leu Asp Lys

485 490 495

Ile Phe Asp Glu Ile Asp Arg Asn Thr Asn Val Pro Ala Lys Thr Asp

500 505 510

Tyr Ser Phe Asp Ile Ile Arg

515

<210> 58

<211> 621

<212> DNA

<213> Unknown

<220>

<223> New strain

<400> 58

gtggagtccg gtggtcctga gccgggcgtg ggctgcgccg gccgcggcgt gatcacctcc 60

atcaacttcc tggaggagaa cggcgcctac gaggacatcg actatgtgtc ctacgacgtg 120

ctgggcgacg tggtgtgcgg cggcttcgcc atgcccatcc gcgagaacaa ggcgcaggaa 180

atctacatcg tgatgtccgg cgagatgatg gccatgtatg cggccaacaa catctccaag 240

ggcatcctga agtatgccaa ttccggcggc gtgcgcctgg gcgggctggt ctgcaacgag 300

cgccagaccg acaaggagct ggagctggcg gaggctctgg cgaagaagct cggcaccgag 360

ctgatctact tcgtgccgcg cgacaacatc gtgcagcatg ccgagctgcg ccgcatgaca 420

gtgatcgagt atgcgcccga ttccgcccag gcccagcact accggaacct ggccgagaag 480

gtgcacgcca acaagggcaa cggcatcatc ccgaccccga tcaccatgga cgagctggaa 540

gacatgctca tggagcacgg catcatgaag gccgtggacg agagccagat cggcaagacc 600

gccgccgagc tcgccgtctg a 621

<210> 59

<211> 206

<212> Protein

<213> Unknown

<220>

<223> New strain

<400> 59

Met Glu Ser Gly Gly Pro Glu Pro Gly Val Gly Cys Ala Gly Arg Gly

1 5 10 15

Val Ile Thr Ser Ile Asn Phe Leu Glu Glu Asn Gly Ala Tyr Glu Asp

20 25 30

Ile Asp Tyr Val Ser Tyr Asp Val Leu Gly Asp Val Val Cys Gly Gly

35 40 45

Phe Ala Met Pro Ile Arg Glu Asn Lys Ala Gln Glu Ile Tyr Ile Val

50 55 60

Met Ser Gly Glu Met Met Ala Met Tyr Ala Ala Asn Asn Ile Ser Lys

65 70 75 80

Gly Ile Leu Lys Tyr Ala Asn Ser Gly Gly Val Arg Leu Gly Gly Leu

85 90 95

Val Cys Asn Glu Arg Gln Thr Asp Lys Glu Leu Glu Leu Ala Glu Ala

100 105 110

Leu Ala Lys Lys Leu Gly Thr Glu Leu Ile Tyr Phe Val Pro Arg Asp

115 120 125

Asn Ile Val Gln His Ala Glu Leu Arg Arg Met Thr Val Ile Glu Tyr

130 135 140

Ala Pro Asp Ser Ala Gln Ala Gln His Tyr Arg Asn Leu Ala Glu Lys

145 150 155 160

Val His Ala Asn Lys Gly Asn Gly Ile Ile Pro Thr Pro Ile Thr Met

165 170 175

Asp Glu Leu Glu Asp Met Leu Met Glu His Gly Ile Met Lys Ala Val

180 185 190

Asp Glu Ser Gln Ile Gly Lys Thr Ala Ala Glu Leu Ala Val

195 200 205

<---

Claims (69)

1. An isolated bacterial strain of the genus Xanthobacter, deposited under the number VTT-E-193585, suitable for use in feed and food products. 2. A culture suitable for use in feed and food products, containing a bacterial strain according to claim 1 and a liquid medium containing ammonium salts, phosphates, sulfates, potassium, sodium, vanadium, iron, magnesium, calcium, molybdenum, manganese, boron salts , zinc, cobalt, selenium, iodine, copper or nickel. 3. A method for producing biomass, including cultivating a bacterial strain according to claim 1. 4. The method of claim 3, comprising cultivating the bacterial strain in a continuous culture with hydrogen as an energy source and an inorganic carbon source, wherein the inorganic carbon source includes carbon dioxide. 5. The method according to claim 4, characterized in that the content of dissolved oxygen in the culture is maintained at a level of 5 to 10%. 6. The method according to claim 4 or 5, characterized in that ammonia, urea, nitrate and/or nitrogen gas are used as a source of nitrogen. 7. The method according to claim 3, characterized in that the minerals contain less than 1 g/l of chlorides, for example, less than 0.25 g/l of chloride salts, or less than 0.1 g/l of chlorides, preferably do not contain chlorides. 8. The method according to claim 7, characterized in that the culture medium does not contain vitamins. 9. The method according to claim 3, characterized in that the pH value in the culture is maintained in the range from 5.5 to 8.0, or from 6.5 to 7.0, such as 6.8. 10. The method according to claim 3, characterized in that said crop is grown at a temperature of 25°C to 40°C, or from 28°C to 32°C, such as 30°C. 11. The method according to claim 3, characterized in that the bacterial strain has a growth rate of 0.04-0.12 h-1. 12. The method according to claim 3, characterized in that the bacterial strain contains 16S ribosomal RNA specified in SEQ ID NO:1. 13. The method according to claim 3, characterized in that the bacterial strain contains: i) a gene encoding a large chain ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) having the sequence shown in SEQ ID NO:3, and ii) a gene encoding small chain ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) having the sequence shown in SEQ ID NO:5, And iii) a gene encoding the alpha subunit of NAD+ reducing hydrogenase HoxS having the sequence shown in SEQ ID NO:7, And iv) a gene encoding the beta subunit of NAD+ reducing hydrogenase HoxS having the sequence shown in SEQ ID NO:9, And v) a gene encoding the gamma subunit of NAD+ reducing hydrogenase HoxS having the sequence shown in SEQ ID NO:11, and vi) a gene encoding the delta subunit of NAD+ reducing hydrogenase HoxS having the sequence shown in SEQ ID NO:13 And vii) a gene encoding a large subunit of NiFeSe hydrogenase having the sequence shown in SEQ ID NO:15, and viii) a gene encoding a small subunit of NiFeSe hydrogenase having the sequence shown in SEQ ID NO:17, And ix) a gene encoding the gamma chain of ATP synthase atpG_1 having the sequence specified in SEQ ID NO:19, And x) a gene encoding the alpha subunit of ATP synthase atpA_1 having the sequence specified in SEQ ID NO:21, And xi) a gene encoding ATP synthase subunit b atpF_1 having the sequence shown in SEQ ID NO:23, And xii) a gene encoding subunit c of sodium ion-specific ATP synthase atpE_1 having the sequence set forth in SEQ ID NO:25, and xiii) a gene encoding the aATP synthase subunit atpB_1 having the sequence specified in SEQ ID NO:27, And xiv) a gene encoding the epsilon chain of ATP synthase atpC_1 having the sequence shown in SEQ ID NO: 29, And xv) a gene encoding the beta subunit of ATP synthase atpD_1 having the sequence specified in SEQ ID NO:31, or a sequence having more than 84% sequence identity with the sequence presented in SEQ ID NO:31, and xvi) a gene encoding the beta subunit of ATP synthase atpD_2 having the sequence specified in SEQ ID NO:33, And xvii) a gene encoding the gamma chain of ATP synthase atpG_2 having the sequence specified in SEQ ID NO:35, And xviii) a gene encoding the alpha subunit of ATP synthase atpA_2 having the sequence shown in SEQ ID NO: 37, And xix) a gene encoding the delta subunit of ATP synthase atpH having the sequence shown in SEQ ID NO:39, And xx) a gene encoding subunit b of ATP synthase atpF_2 having the sequence specified in SEQ ID NO:41, And xxi) a gene encoding the b' subunit of ATP synthase atpG_3, having the sequence specified in SEQ ID NO:43, And xxii) a gene encoding the cATP synthase subunit atpE_2 having the sequence specified in SEQ ID NO:45, And xxiii) a gene encoding the ATP synthase subunit atpB_2 having the sequence shown in SEQ ID NO:47, And xxiv) a gene encoding ATP synthase protein I atpI having the sequence shown in SEQ ID NO: 49, And xxv) a gene encoding the alpha chain of molybdenum-iron protein nitrogenasenifD_1, having the sequence specified in SEQ ID NO:51, And xxvi) a gene encoding the alpha chain of molybdenum-iron nitrogenase protein nifD_2, having the sequence specified in SEQ ID NO:53, And xxvii) a gene encoding the molybdenum-iron beta chain of the nitrogenase protein nifK_1, having the sequence specified in SEQ ID NO:55, And xxviii) a gene encoding the molybdenum-iron beta chain of the nitrogenase protein nifK_2, having the sequence specified in SEQ ID NO: 57, And xxix) a gene encoding iron nitrogenase protein nifH having the sequence shown in SEQ ID NO: 59. 14. The method of claim 3, including the additional step of collecting biomass obtained during cultivation, optionally including the additional step of drying the biomass. 15. A method for obtaining protein, which involves obtaining biomass, including cultivating the bacterial strain according to claim 1, collecting the biomass obtained during cultivation, and an additional step of isolating protein from said biomass. 16. The method according to claim 14 or 15, including the additional step of obtaining a food product or feed product from the specified biomass.