RU2843065C1 - Laboratory automatic syringe micro-injector, method of its assembly, and method of its use - Google Patents

Abstract

FIELD: measuring.

SUBSTANCE: inventions are presented, one of which relates to a group of devices called "laboratory syringe injectors", is a device for accurate dosing of medicinal preparations when conducting biological experiments and surgical operations mainly on animals. Wherein, this device has the ability to be electrically grounded, which makes it possible to use it when conducting electrophysiological experiments. Method for assembling said laboratory automatic syringe micro-injector and a method for use thereof are also provided. Essential features of the inventions are described in corresponding independent claims. Technical problem solved by the claimed inventions and their technical result is the development of a laboratory automatic syringe micro-injector eliminating the disadvantages of analogues, namely: 1 – providing the possibility of automatic short and long injections of drugs both with high and low rate of administration with a low discrete interval due to the use of the rotor rotation of the stepper motor as a driving force; 2 – enabling the implementation of multi-stage injections due to the programmable control unit and graphical interface; 3 – providing the possibility of automatic and programmable intake of the required volume of the drug preparation at a certain speed, which reduces its consumption, due to the double-sided syringe fastening system in the injector unit and the graphical interface functionality; 4 – providing the possibility of semi-automatic calibration for a certain syringe, which increases the accuracy of injections due to the use of a stepper motor and a graphical interface functionality; 5 – enabling the use of the device when conducting biological experiments and surgical operations on animals, which is achieved by the syringe fastening system, designed for syringes of both large (5 ml) and small volume (0.5 ml); 6 – possibility of using the device when conducting electrophysiological experiments on animals, which is achieved due to the absence of electromagnetic interference, eliminated by grounding the control unit through a grounding contact.

EFFECT: invention provides the possibility of automatic dosed intake from containers and multiple introduction of drug preparations into animal tissues by means of syringes of various types.

3 cl, 20 dwg

Description

The claimed device belongs to a group of devices called "laboratory syringe injectors", is a device for precise dosing of drugs during biological experiments and surgical operations mainly on animals, providing the possibility of automatic dosed withdrawal from containers and multiple injections into animal tissues of drugs using syringes of different types. At the same time, this device has the ability to be electrically grounded, which makes it possible to use it during electrophysiological experiments.

Further in the text, the applicant provides terms that are necessary to facilitate a clear understanding of the essence of the declared materials and to eliminate contradictions and/or controversial interpretations when performing an examination on the merits.

Syringe flange - a flat ellipsoidal protrusion on the rear of the syringe plunger that serves as a finger rest.

Discrete interval - in the context of this description, the smallest possible angular displacement of the motor axis that corresponds to the minimum linear displacement of the carriage.

A four-pin male connector is an electrical device designed for mechanical connection and disconnection of two electrical circuits.

Grounding contact - an electrical element intended for electrical grounding of any device through it. In the context of this description, it implements an electrical contact between the minus of the power supply of the described device and the common ground of the equipment for conducting electrophysiological experiments, which is usually a high-capacity conductor, such as a metal table.

Semi-automatic calibration - in the context of this description, is the calculation by the device of a calibration coefficient for a specific type of syringe with the participation of the operator (user).

From the studied level of technology, the applicant identified an invention according to patent RU 2673983 C1 “Syringe dispenser for medicines”. The essence of the known device is a syringe dispenser of medicines, including a housing with the possibility of installing and fixing a syringe with a piston on it, a piston pusher driven by a DC motor, as well as a power supply unit and a control unit consisting of a controller, a control panel and a display for displaying the infusion parameters, characterized in that it is additionally equipped with a movable clamping rod located perpendicular to the longitudinal axis of the syringe, on the upper end of which a clamp is installed, and on the lower end - a slider connected to a movable rod of a potentiometer, the signal from which is fed to the control unit via a bus, the syringe piston pusher is fixed on a threaded shaft on which a motor revolution counter is installed, including a disk diaphragm rotating on the threaded shaft with radial slots, located between the LED and the phototransistor for monitoring the passage of the light flux, while the threaded shaft is mounted on the housing using a latch located in its upper part, as well as an end and a through bushings, and a locking wall is secured between the diaphragm and the through bushing for fixing the diaphragm on the motor shaft. A device characterized in that the disk diaphragm is connected to the motor through a transition bushing to ensure the possibility of its rotation. A device characterized in that a continuous-rotation DC motor is used as the motor. A device characterized in that the housing is equipped with a rear protective wall and fasteners for fixing it on the patient's hand. A device characterized in that the overall dimensions of the dispenser are 167 × 92 × 32 mm and weighs 0.3 kg. At the same time, this device is mainly used in the medical field, which is characterized by the use of syringes of large diameters and volumes: 5 ml, 10 ml, 20 ml. The device is controlled through a graphical interface with the ability to set the injection speed.

Briefly, the essence is a syringe dispenser of drugs, which carries out the introduction of drugs using a DC motor with the ability to set the infusion rate. At the same time, this device has the ability to automatically determine the diameter of the syringe used. This device is mainly used in the field of medicine.

The disadvantages of the known technical solution in relation to the design and intended use are:

- there is no possibility of implementing multi-stage injections, since this function is absent in the graphical interface of the known device;

- there is no possibility of automatic and programmable selection of the required volume of the drug at a certain speed, since there is no two-way system for fastening syringes in the injector unit and this function is not available in the graphical interface of the known device;

- there is no possibility of using the device when conducting biological experiments and surgical operations on animals, since the known device belongs to the field of medicine and is designed for use with humans and implements the introduction of only large volumes (5 ml, 10 ml, 20 ml) using syringes;

- it is not possible to use the device when conducting electrophysiological experiments on animals, since there is no grounding of the control unit via the grounding contact.

From the studied prior art, the applicant has identified a utility model according to patent CN 213175954 U "Laboratory injection pump". The essence of the known device is a laboratory injection pump, which contains a housing, a control board and a power source located in the housing, a touch screen is located outside the housing, a driving device is located in the upper part of the housing, a clamping device is located on the driving device, the driving device consists of two locking blocks, and two locking blocks are fixed on two sides of the upper part of the housing, respectively. A linear shaft is located between the two locking blocks, a lead screw is located between the two linear shafts, two ends of the lead screw are rotationally connected to the two locking blocks, a sliding block penetrates the lead screw and is in a threaded connection with the lead screw, two ends of the sliding block are in a sliding connection with two linear shafts, respectively, and grooves are formed on the side surfaces of the two ends of one linear shaft in the circumferential direction. The gasket is inserted into the groove, so that the linear shaft is in an elastic floating state and can move radially in a certain range, the running noise and the phenomenon of clamp stagnation are eliminated, and the overall sliding balance of the sliding block and the stability after loading are not affected. At the same time, the movement of the driving device is realized by a stepping motor.

Briefly, the essence is a laboratory injection pump that administers the drug by moving a sliding block along linear shafts and has a special elastic gasket that makes the shaft elastically floating. The movement of the sliding block itself is carried out by the operation of a stepper motor that rotates a threaded shaft connected to the sliding block via a threaded connection.

The disadvantages of the known technical solution in relation to the design and intended use are:

- there is no possibility of implementing multi-stage injections, since this function is not specified as a function of the graphical interface of the known device;

- there is no possibility of automatic and programmable dialing of the required volume of the drug at a certain speed, since there is no two-way system for fastening syringes in the injector unit and this function is not indicated as a function of the graphical interface of the known device;

- there is no possibility of semi-automatic calibration for a specific syringe, since this function is not indicated as a function of the graphical interface of the known device;

- it is not possible to use the device when conducting electrophysiological experiments on animals, since there is no grounding of the control unit via the grounding contact.

From the studied prior art, the applicant has identified an invention according to patent US 2004057855 A1 "Syringe pump". The essence of the known device is a syringe pump consisting of a housing with a syringe groove for placing a syringe in it; a drive head for moving the syringe piston, wherein the drive head is made with the possibility of linear movement relative to the housing; a control panel connected to the housing; and a rotating cover covering at least part of the syringe groove, wherein the rotating cover contains a magnifying device for an enlarged image of the syringe surface, wherein the magnifying device is capable of optically enlarging the surface of a syringe of different sizes, the diameters of which differ by at least 2 times. The device, characterized in that the magnifying device contains an elongated cylindrical lens. The device, characterized in that the cover contains a lighting device for illuminating the syringe in the syringe groove. The device, characterized in that the syringe groove contains a lighting device for illuminating the syringe in the syringe groove. A device characterized in that the cover includes a control panel and a display device.

Briefly, the essence is a syringe pump that performs long-term infusions of drugs due to the movement of the drive head. At the same time, the device has a magnifying device for an enlarged image of the syringe surface and the ability to illuminate the syringe in the syringe groove.

The disadvantages of the known technical solution in relation to the design and intended use are:

- there is no possibility of automatic injections of drugs at a high rate of administration, since the device is designed for slow injections;

- there is no possibility of implementing multi-stage injections, since this function is absent in the graphical interface of the known device;

- there is no possibility of automatic and programmable dialing of the required volume of the drug at a certain speed, since this function is not available in the graphical interface of the known device;

- there is no possibility of using the device when conducting biological experiments and surgical operations on animals, since the known device belongs to the field of medicine and is designed for use with humans and implements the introduction of only large volumes (5 ml, 10 ml, 20 ml) using syringes;

- it is not possible to use the device when conducting electrophysiological experiments on animals, since there is no grounding of the control unit via the grounding contact.

From the studied prior art, the applicant has identified an invention according to patent AU 2021241336 A1 "Syringe pump" "Syringe pump". The essence of the known device is a syringe pump for dosing liquid from a syringe, including a hollow syringe body and a plunger movable in the syringe body. The body contains a motor and an electronic controller. The syringe is attached to the body to prevent relative movement of the syringe and the body. The drive is movably mounted on the body relative to the syringe mount and engages the syringe plunger in such a way that when the drive moves, the syringe plunger enters or exits the syringe body. The mount may include a screen to protect the syringe from the environment. In other configurations, the syringe holder body allows several syringe pumps to be installed/assembled together. In other configurations, all pump components are located in a conditional shell defined by the outer boundaries of the body. Part of the functionality of this device is the ability to adjust the shape of the injection flow curve over time by connecting this device to a computer. This device also has the ability to launch an injection via Bluetooth and WiFi. At the same time, the known device is designed for the purpose of using small-sized syringes (<2.5 ml).

Briefly, the essence is a syringe pump that allows for the collection and administration of drugs by moving the syringe piston with a piston pusher using a motor. This device has the ability to install several identical devices on top of each other. The device is designed for the use of small syringes (<2.5 ml).

The disadvantages of the known technical solution in relation to the design and intended use are:

- there is no possibility of implementing multi-stage injections, since this function is absent in the graphical interface of the known device;

- there is no possibility of semi-automatic calibration for a specific syringe, since this function is not indicated as a function of the graphical interface of the known device;

- it is not possible to use the device when conducting electrophysiological experiments on animals, since there is no grounding of the control unit via the grounding contact.

The identified analogues coincide with the declared technical solution in terms of individual matching features, therefore the prototype was not identified and the invention formula was drawn up without a limiting part.

The technical problem solved by the claimed invention and its technical result is the development of a laboratory automatic syringe microinjector that eliminates the shortcomings of analogues, namely:

1 - providing the possibility of automatic short and long injections of medicinal preparations, both at high and low rates of administration with a low discrete interval, by using the rotation of the rotor of a stepper motor as a driving force;

2 - ensuring the possibility of implementing multi-stage injections due to a programmable control unit and graphical interface;

3 - providing the ability to automatically and programmably dial the required volume of the drug at a certain speed, which reduces its consumption, due to the two-way system of fastening syringes in the injector block and the functionality of the graphical interface;

4 - providing the possibility of semi-automatic calibration for a specific syringe, which increases the accuracy of injections, due to the use of a stepper motor and the functionality of the graphical interface;

5 - ensuring the possibility of using the device when conducting biological experiments and surgical operations on animals, which is achieved through a syringe attachment system designed for both large (5 ml) and small volume (0.5 ml) syringes;

6 - ensuring the possibility of using the device when conducting electrophysiological experiments on animals, which is achieved due to the absence of electromagnetic interference, eliminated by grounding the control unit through the ground contact.

The essence of the claimed technical solution is a laboratory automatic syringe microinjector consisting of a stepper motor, an injector unit, a control unit, a power source, and a graphical interface; the stepper motor consists of a housing, a rotor, holes for screws, and winding contacts; the rotor of the stepper motor is located inside its housing in the center; the holes for the stepper motor screws are located on its front face, when the stepper motor is fixed in the injector unit, the contacts of the stepper motor windings are located on its side face; the injector unit consists of a rear, middle, and front fragments, guide cylinders, a threaded stud, a carriage, a syringe, fixing screws, and a bearing; on the rear face of the rear fragment of the injector unit there is a recess for the stepper motor housing, and on the front face there is a positional recess of the rear fragment; in the center of the rear fragment there is a through hole for the rotor of the stepper motor, and along the edges of its front and rear faces there are through holes for the screws of the rear fragment; in the center of the middle fragment of the injector block there is a channel for the carriage, open upwards; on the inner side of the walls of this channel there are recesses for the guide cylinders, and on its front and rear ends there are guide cylinder locks made in the form of elongated rings; on the middle fragment along the edges of its end extensions there are through front and rear holes for the screws of the middle fragment; on the rear face of the front fragment of the injector block there is a positional recess of the front fragment, and on the bottom of this recess there is a recess for the bearing in the center; recesses for screws are made along the edges of the rear face of the front fragment; a syringe flange lock is made on the upper face of the front fragment; Fixing screws are installed through the rear holes for the screws of the middle fragment, the holes for the screws of the rear fragment and the holes for the screws of the stepper motor with the possibility of fastening together the middle fragment of the injector unit, the rear fragment of the injector unit and the stepper motor housing, wherein the rear end of the middle fragment is inserted into the position recess of the rear fragment of the injector unit, and the stepper motor housing is immersed in the recess for the stepper motor housing; fixing screws are installed through the front holes for the screws of the middle fragment in the recesses for the screws of the front fragment, wherein the front end of the middle fragment is inserted into the position recess of the front fragment; guide cylinders are located in the recesses for the guide cylinders of the middle fragment and are fixed there by means of guide cylinder clamps; the threaded stud is connected to the bearing by means of a cylindrical connector, and to the rotor of the stepper motor by means of a coupling, in the latter recesses for the threaded stud, a recess for the rotor of the stepper motor and holes for the mounting screws are made; in the central part of the carriage there is a central opening for a connecting cylinder with the possibility of connecting the connecting cylinder of the carriage with a threaded stud to form a "screw-nut" transmission between them; on the side and lower edges of the carriage there are guide half rings with the possibility of forming a sliding contact with the guide cylinders; on the upper edge of the carriage there is a cutout for the piston retainer and a clamping mechanism, the latter consisting of a clamping mechanism stud installed in the corresponding opening and connected to the clamping plate in the cutout of the piston retainer; the syringe is fixed in the injector unit by its flange in the syringe flange retainer and by the piston in the cutout of the piston retainer using the clamping mechanism; the bearing is located in the recess for the bearing on the front fragment of the injector unit; the control unit consists of a housing, a display, tact buttons, a pin four-pin connector, a power socket, a toggle switch, a grounding contact and a control system; on the lower edge of the control unit housing there are holes with control unit screws with the possibility of fastening its upper and lower parts; the display, which is an information output device, and the tact buttons - information input devices, are mounted in the control unit on its upper edge; a number of elements are made on the rear edge of the control unit: a four-pin pin connector to which a wire is connected going to the contacts of the stepper motor windings; a power socket to which a power adapter coming from a power source is connected; a toggle switch with the possibility of turning the power of the device on/off; a grounding contact; the control system is designed with the possibility of ensuring rotation of the stepper motor rotor and reproducing instructions specified by the user via the tact buttons, and consists of: a microcontroller board, a stepper motor driver with the possibility of transmitting power from the power source to the stepper motor windings in a certain sequence via output contacts - using a four-pin pin connector, wires and a contact of the stepper motor windings; the power source consists of a 12 V power adapter and a voltage stabilizer; a 12 V power adapter is connected to the control unit via a power connector with the ability to supply power to the stepper motor windings with a voltage of 12 V via its driver, as well as with the ability to supply power to the microcontroller, tact buttons and display with a voltage of 5 V via a voltage stabilizer; the graphical interface is a sequence of software windows displayed on the display, with the ability to interact with them by means of tact buttons, and is configured to perform the following operations: performing calibration for a certain type of syringe, free movement of the carriage, moving the carriage to the zero position, drawing liquid into the syringe at a certain speed and a specified volume, injecting the contents of the syringe at a certain speed and a specified volume. A method for assembling a laboratory automatic syringe microinjector according to claim 1, consisting in taking pre-fabricated rear, middle and front fragments of the injector unit, a cylindrical connector, a coupling, a control unit housing, a carriage; a stepper motor is attached to the rear fragment of the injector unit without fixing with screws so that the contacts of the stepper motor windings are on the right, wherein the stepper motor housing is immersed in a recess for the stepper motor housing, wherein the stepper motor rotor passes through the opening for the stepper motor rotor; a connecting cylinder is inserted into the central opening of the carriage, guide half rings are fixed to the carriage; a threaded stud is screwed into the connecting cylinder up to half its length; a cylindrical connector is screwed onto the front end of this stud; the assembled structure is inserted into the channel for the carriage so that the cylindrical connector faces the front end of the middle fragment; a coupling is installed on the rotor of the stepper motor with the end containing a recess for the rotor of the stepper motor, wherein the rotor of the stepper motor is fixed in this recess by means of set screws through the holes for the set screws; the middle fragment is installed with its rear end into the position recess of the rear fragment, wherein the free end of the threaded stud is immersed into the recess for the threaded stud in the coupling and secured there using set screws through the holes for the set screws; through the rear holes for the screws of the middle fragment, the holes for the screws of the rear fragment and the holes for the screws of the stepper motor, the fixing screws are screwed in, fastening together the middle fragment of the injector unit, the rear fragment of the injector unit and the stepper motor housing, respectively; the guide cylinders are inserted into the recesses for the guide cylinders through the locks of the guide cylinders until they stop, wherein a sliding contact is formed between them and the guide half rings, respectively; a bearing is taken, pressed into the recess for the bearing of the front fragment; the front end of the middle fragment is installed in the position recess of the front fragment, wherein the cylindrical connector with its front edge enters the inner ring of the bearing; The fixing screws are screwed through the front screw holes of the middle fragment into the recesses for the screws of the front fragment; the clamping mechanism is assembled by screwing the clamping mechanism pin into the hole for the clamping mechanism pin, while in the cutout of the piston retainer this pin is connected to the clamping plate; soldering equipment is taken and a working circuit is assembled from a microcontroller, a stepper motor driver, a four-pin pin connector, a power jack, a toggle switch, a ground contact, a voltage stabilizer, a display, and tact buttons; the display and tact buttons are taken and mounted into the control unit from above; the power jack, four-pin pin connector, toggle switch and ground contact are mounted into the control unit housing from the rear; the power adapter is connected on one side to the power source, and on the other side to the power jack; the electrical connection between the control unit and the stepper motor is made with a four-wire cable, wherein each wire goes to the contact of a separate winding of the stepper motor, which is connected from the stepper motor side to the contacts of the stepper motor windings, and from the control unit side is connected to a four-pin connector. The method of using the laboratory automatic syringe microinjector according to claim 1, consisting in the fact that in order to start operation, the power supply adapter is connected to the power source and to the power socket, wherein the control unit is placed outside the experimental setup, and the injector unit is located in close proximity to the animal being tested; the control unit and the stepper motor are connected with a cable, wherein it is connected from the control unit side to the four-pin connector, and from the stepper motor side to the contacts of the stepper motor windings; the device as a whole is switched on, which is accomplished by moving the toggle switch to the "ON" state, wherein the device switch-on indicator is the appearance of a graphical interface on the display; The laboratory automatic syringe microinjector is calibrated for the required type of syringe using a test syringe, which is a syringe identical to the syringe that will be subsequently used to perform the injection; the operator draws a certain amount of fluid into the test syringe for calibration; the operator then interacts with the graphical interface using the tact buttons on the display and selects the "Calibre" submenu, then selects the "Movement" line, then "Manual"; after that, by pressing the tact buttons, the operator moves the carriage to the required position to match the test syringe piston; the test syringe is installed with its flange in the syringe flange retainer and with its piston in the cutout of the piston retainer and fixed there with a clamping mechanism. This operation is performed by tightening the clamping mechanism pin until the clamping plate tightly contacts the syringe piston; the operator then selects the "Start K." and "Start" items, after which the carriage begins a slow forward movement, displacing the fluid from the syringe; Then the operator presses the "Stop" button on the display, the movement stops, and the program asks the operator to specify the displaced volume of liquid; the displaced volume of liquid is measured based on the marks on the body of the syringe or using the mass of the displaced liquid; after specifying the obtained volume, the microcontroller converts the obtained value of the liquid volume in accordance with the number of steps performed by the stepper motor and writes the calibration coefficient to the memory, thus, the calibration process is considered complete; in this case, if the calibration has already been performed earlier and it is planned to use the same model of syringe, the operator selects "Previous K.", and the program will use the calibration coefficient calculated during the previous calibration; the test syringe is removed and the carriage is moved to the initial position by selecting the "To zero"item; a working syringe is installed in place of the test syringe according to the algorithm described above, while in order to exclude errors, air is preliminarily displaced from the dead space of the syringe; the substance is drawn into the working syringe by pressing the "Draw" item button on the graphical interface; then the operator specifies the required volume and speed of drawing up the drug; two actions are configured in the "Actions" submenu of the graphical interface, with the operator setting the required volume, time and rate of administration for each action: the first stage is the administration of part of the collected volume at the maximum rate for inducing anesthesia, the second stage is the administration of the remaining volume over two hours; after completing the specified setting and pressing the "Start" item, all specified actions are executed in the order they are specified; in order to eliminate electromagnetic interference when recording an electrical signal by the equipment for conducting an electrophysiological experiment, the ground contact of the control unit is connected to the ground of this equipment; then a carrier for delivering the drug to the animal is installed on the end of the syringe; then the operator selects the "Start" item, starting the execution of all specified actions, while the microcontroller converts the specified actions and sends this information to the stepper motor driver, which, acting as a "gate mechanism", converts them into a sequence of pulses sent from the power adapter to the stepper motor windings, this causes the rotor of the stepper motor to rotate, which is transmitted through the coupling to the threaded stud and then by means of the connecting cylinder - to the carriage; the rotation of the latter is limited by the joint formed by the guide half rings and guide cylinders, due to which there is a linear movement of the carriage at a speed specified by the operator for a certain distance corresponding to the set injection volume; after the completion of the first action, the execution of the next action begins according to the same principle.

The claimed technical solution is illustrated in Fig.1 - Fig.20.

Fig. 1 shows a part of the claimed device - the injector block (isometric projection).

Fig. 2 shows a part of the claimed device - a stepper motor (isometric projection).

Fig. 3 shows a part of the claimed device - the rear fragment of the injector block (isometric projection).

Fig. 4 shows a part of the claimed device - the rear fragment of the injector unit (rear view).

Fig. 5 shows a part of the claimed device - the rear fragment of the injector block (front view).

Fig. 6 shows a part of the claimed device - the middle fragment of the injector block (isometric projection).

Fig. 7 shows a part of the claimed device - the middle fragment of the injector block (side view).

Fig. 8 shows a part of the claimed device - the front fragment of the injector block (isometric projection).

Fig. 9 shows a part of the claimed device - the front fragment of the injector block (rear view).

Fig. 10 shows a part of the claimed device - the front fragment of the injector block (front view).

Fig. 11 shows a part of the claimed device - a carriage (isometric projection).

Fig. 12 shows a part of the claimed device - a carriage (front view).

Fig. 13 shows a part of the claimed device - a coupling (isometric projection).

Fig. 14 shows a part of the claimed device - a coupling (front view).

Fig. 15 shows a part of the claimed device - an injector unit without a stepper motor (side view).

Fig. 16 shows a part of the claimed device - an injector unit without a stepper motor (front view).

Fig. 17 shows a part of the claimed device - a control unit (isometric projection).

Fig. 18 shows a part of the claimed device - the control unit (bottom view).

Fig. 19 shows a part of the claimed device - the control unit (rear view).

Fig. 20 shows a block diagram of the control unit and power supply.

The positions on the figures indicate:

1 - Stepper motor

1.1 - Stepper motor housing

1.2 - Stepper motor rotor

1.3 - Stepper Motor Screw Holes

1.4 - Stepper motor winding contacts

2 - Injector block

2.1 - Rear fragment of the injector block

2.1.1 - Recess for stepper motor housing

2.1.2 - Hole for stepper motor rotor

2.1.3 - Positional deepening of the posterior fragment

2.1.4 - Posterior Fragment Screw Holes

2.2 - Middle fragment of the injector block

2.2.1 - Carriage channel

2.2.2 - Recesses for guide cylinders

2.2.3 - Guide cylinder locks

2.2.4 - Rear screw holes for the middle fragment

2.2.5 - Front holes for screws of the middle fragment

2.3 - Front fragment of the injector block

2.3.1 - Positional recess of the anterior fragment

2.3.2 - Bearing recess

2.3.3 - Syringe flange retainer

2.3.4 - Anterior fragment screw recesses

2.4 - Guide cylinders

2.5 - Threaded stud

2.5.1 - Cylindrical connector

2.5.2 - Coupling

2.5.2.1 - Recess for threaded stud

2.5.2.2 - Recess for stepper motor rotor

2.5.2.3 - Holes for mounting screws

2.6 - Carriage

2.6.1 - Central hole

2.6.2 - Guide half rings

2.6.3 - Connecting cylinder

2.6.4 - Piston retainer cutout

2.6.5 - Clamping mechanism

2.6.5.1 - Clamping mechanism pin hole

2.6.5.2 - Clamping mechanism pin

2.6.5.3 - Pressure plate

2.7 - Syringe

2.7.1 - Syringe plunger

2.7.2 - Syringe flange

2.8 - Fixing screws

2.9 - Bearing

3 - Control unit

3.1 - Control unit housing

3.1.1 - Control unit screw holes

3.2 - Display

3.3 - Tact buttons

3.4 - Four-pin male connector

3.5 - Power socket

3.6 - Toggle Switch

3.7 - Grounding contact

3.8 - Control system

3.8.1 - Microcontroller

3.8.2 - Stepper Motor Driver

4 - Power supply

4.1 - Power adapter

4.2 - Voltage stabilizer.

The applicant then provides a description of the claimed technical solution.

The claimed device for implementing the method functionally consists of the following main structural parts (Fig. 1 - Fig. 20) :

- stepper motor (1),

- injector block (2),

- control unit (3),

- power supply (4),

- graphical interface (represents software for the operation of the declared technical solution, it is described briefly for a general understanding of the operating principle of the declared technical solution and is an integral part of it when used for its intended purpose and is developed individually for the declared design).

1. The stepper motor (1) (see Fig.1 - Fig.2) consists of its housing (1.1), rotor (1.2), holes for screws (1.3) and winding contacts (1.4).

The rotor of the stepper motor (1.2) is located inside its housing (1.1) in the center. The holes for the screws of the stepper motor (1.3) are located on its front edge. When fixing the stepper motor (1) in the injector block (2), the contacts of the windings of the stepper motor (1.4) are located on its side edge.

2. The injector block (2) (Fig. 1, Fig. 3 - Fig. 16) consists of rear (2.1), middle (2.2) and front (2.3) fragments, cylinder guides (2.4), threaded stud (2.5), carriage (2.6), syringe (2.7), fixing screws (2.8) and bearing (2.9).

On the rear face of the rear fragment of the injector unit (2.1) there is a recess for the stepper motor housing (2.1.1), and on the front face there is a positional recess of the rear fragment (2.1.3). In the center of the rear fragment (2.1) there is a through hole for the rotor of the stepper motor (2.1.2), and along the edges of its front and rear faces there are through holes for the screws of the rear fragment (2.1.4).

In the center of the middle fragment of the injector block (2.2) there is a channel for the carriage (2.2.1), open upwards. On the inner side of the walls of this channel (2.2.1) there are recesses for the guide cylinders (2.2.2), and on its front and rear ends there are guide cylinder locks (2.2.3) made in the form of elongated rings. Also on the middle fragment (2.2) along the edges of its end extensions there are through front (2.2.5) and rear (2.2.4) holes for the screws of the middle fragment.

On the rear face of the front fragment of the injector block (2.3) there is a positional recess of the front fragment (2.3.1), and on the bottom of this recess (2.3.1), in the center, there is a recess for the bearing (2.3.2). Along the edges of the rear face of the front fragment (2.3) there are recesses for screws (2.3.4). On the upper face of the front fragment (2.3) there is a retainer for the syringe flange (2.3.3).

Fixing screws (2.8) are installed through the rear holes for the screws of the middle fragment (2.2.5), the holes for the screws of the rear fragment (2.1.4) and the holes for the screws of the stepper motor (1.3) with the possibility of fastening together the middle fragment of the injector block (2.2), the rear fragment of the injector block (2.1), and the stepper motor housing (1.1). In this case, the rear end of the middle fragment (2.2) is inserted into the positional recess of the rear fragment of the injector block (2.1.3), and the stepper motor housing (1.1) is immersed in the recess for the stepper motor housing (2.1.1).

Through the front holes for the screws of the middle fragment (2.2.5), the fixing screws (2.8) are installed into the recesses for the screws of the anterior fragment (2.3.4). In this case, the front end of the middle fragment (2.2) is inserted into the positional recess of the anterior fragment (2.3.1)

The guide cylinders (2.4) are located in the recesses for the guide cylinders (2.2.2) of the middle fragment (2.2) and are secured there by means of the guide cylinder clamps (2.2.3).

The threaded stud (2.5) is connected to the bearing (2.9) by means of a cylindrical connector (2.5.1), and to the rotor of the stepper motor (1.2) by means of a coupling (2.5.2). The latter has recesses for the threaded stud (2.5.2.1), a recess for the rotor of the stepper motor (2.5.2.2) and holes for the mounting screws (2.5.2.3).

In the central part of the carriage (2.6) there is a central opening (2.6.1) for the connecting cylinder (2.6.3) with the possibility of connecting the connecting cylinder (2.6.3) of the carriage (2.6) with the threaded stud (2.5) to form a "screw-nut" transmission between them. On the side and lower edges of the carriage (2.6) there are guide half rings (2.6.2) with the possibility of forming a sliding contact with the guide cylinders (2.4). On the upper edge of the carriage (2.5) there is a cutout for the piston retainer (2.6.4) and a clamping mechanism (2.6.5). The latter consists of a clamping mechanism stud (2.6.5.2) installed in the corresponding opening (2.6.5.1) and connected to the clamping plate (2.6.5.3) in the cutout of the piston retainer (2.6.4).

The syringe (2.7) is fixed in the injector block (2) with its flange (2.7.1) in the syringe flange retainer (2.3.3) and the piston (2.7.2) in the cutout of the piston retainer (2.6.4) using the clamping mechanism (2.6.5)

The bearing (2.9) is located in the bearing recess (2.3.2) on the front section of the injector block (2.3).

3. The control unit (3) (Fig. 17 - Fig. 20) consists of a housing (3.1), a display (3.2), tact buttons (3.3), a four-pin connector (3.4), a power socket (3.5), a toggle switch (3.6), a ground contact (3.7) and a control system (3.8).

On the lower edge of the control unit housing (3.1) there are holes with screws for the control unit (3.1.1) with the possibility of fastening its upper and lower parts.

The display (3.2), which is an information output device, and the tact buttons (3.3), which are information input devices, are mounted in the control unit (3) on its upper edge.

On the rear edge of the control unit (3) there are a number of elements:

- a four-pin male connector (3.4), to which the wire leading to the contacts of the stepper motor windings (1.4) is connected,

- power socket (3.5), to which the power adapter (4.1) coming from the power source (4) is connected,

- toggle switch (3.6) with the ability to turn the device power on/off,

- grounding contact (3.7).

The absence of electromagnetic interference from the described device during the recording of the electrical activity of the excitable tissues of the animal is ensured by the presence of a grounding contact (3.7) on the control unit (3), which allows for the implementation of an electrical connection between the minus of the power supply of the described device and the common ground of the equipment for conducting electrophysiological experiments.

The control system of the device (3.8) is designed to ensure rotation of the rotor of the stepper motor (1.2) and reproduction of instructions specified by the user via the tact buttons (3.3), and consists of:

- microcontroller boards (3.8.1),

- stepper motor drivers (3.8.2) with the ability to transmit power from the power source (4) to the stepper motor windings (1) in a certain sequence via output contacts - using a four-pin pin connector (3.4), wires and the contact of the stepper motor windings (1.4).

4. The power supply (4) (Fig. 20) consists of a 12 V power adapter (4.1) and a voltage stabilizer (4.2). The 12 V power adapter (4.1) is connected to the control unit (3) via a power connector (3.5), with the ability to power the windings of the stepper motor (1) with a voltage of 12 V through its driver (3.8.2), as well as with the ability to power the microcontroller (3.8.1), tact buttons (3.3) and display (3.2) with a voltage of 5 V through the voltage stabilizer (4.2)

5. The graphical interface is a sequence of program windows displayed on the display (3.2), with the ability to interact with them using tact buttons (3.3). This interface is designed with the ability to perform the following operations:

- performing calibration for a specific type of syringe,

- free movement of the carriage,

- moving the carriage to the zero position,

- filling a syringe with liquid at a certain speed and a given volume,

- performing an injection of the contents of a syringe at a certain speed and a given volume.

Below, the applicant provides examples of the implementation of the declared technical solution.

Example 1. The process of assembly (manufacturing) of the declared device.

Take the following parts, pre-fabricated before their assembly, for example, by printing from plastic filament using additive 3D printing technology: the rear (2.1), middle (2.2) and front (2.3) fragments of the injector block (2), the cylindrical connector (2.5.1), the coupling (2.5.2), the control unit housing (3.1), as well as the carriage (2.6), with the exception of the guide half rings (2.6.2), the connecting cylinder (2.6.3) and the clamping mechanism pin (2.6.5.2).

A stepper motor (1), for example, 17HS4401, is attached to the rear fragment of the injector block (2.1), without fixing it with screws (2.8), so that the contacts of the stepper motor windings (1.4) are on the right. In this case, the stepper motor housing (1.1) is immersed in the recess for the stepper motor housing (2.1.1), and the rotor of the stepper motor (1.2) passes through the hole for the rotor of the stepper motor (2.1.2).

A connecting cylinder (2.6.3), for example a steel cylinder with an M6 internal thread, is inserted into the central opening (2.6.1) of the carriage (2.6). Guide half rings (2.6.2), for example, half of a steel coupling without threads, are also fixed on the carriage (2.6). Next, a threaded stud (2.5), for example an M6 steel threaded stud, is screwed into the connecting cylinder (2.6.3) to half its length. Next, a cylindrical connector (2.5.1) is screwed onto the front end of this stud (2.5). Next, the assembled structure is inserted into the channel for the carriage (2.2.1) so that the cylindrical connector (2.5.1) faces the front end of the middle fragment (2.2).

The coupling (2.5.2) is installed on the rotor of the stepper motor (1.2) with the end containing a recess for the rotor of the stepper motor (2.5.2.2). In this case, the rotor (1.2) is fixed in this recess by means of mounting screws, for example, steel mounting screws M3x3, through the holes for the mounting screws (2.5.2.3).

The middle fragment (2.2) is installed with its rear end in the positioning recess of the rear fragment (2.1.3). In this case, the free end of the threaded stud (2.5) is immersed in the recess for the threaded stud (2.5.2.1) in the coupling (2.5.2) and secured there using set screws, for example, steel set screws M3x3, through the holes for the set screws (2.5.2.3).

Through the rear holes for the screws of the middle fragment (2.2.5), the holes for the screws of the rear fragment (2.1.4) and the holes for the screws of the stepper motor (1.3), the fixing screws (2.8) are screwed in, for example, M3 steel screws, which fasten together the middle fragment of the injector block (2.2), the rear fragment of the injector block (2.1), and the housing of the stepper motor (1.1), respectively.

Guide cylinders (2.4), for example steel cylinders with a diameter of 6 mm, are inserted into the recesses for guide cylinders (2.2.2) through the guide cylinder clamps (2.2.3) until they stop. In this case, a sliding contact is formed between them (2.4) and the guide half rings (2.6.2), respectively.

Take a bearing (2.9), for example, bearing 608 zz, and press it into the recess for the bearing (2.3.2) of the front fragment (2.3).

The front end of the middle fragment (2.2) is installed in the positional recess of the front fragment (2.3.1), while the cylindrical connector (2.5.1) with its front edge enters the inner ring of the bearing (2.9).

Through the front holes for the screws of the middle fragment (2.2.5), the fixing screws (2.8), for example, M3 steel screws, are screwed into the recesses for the screws of the anterior fragment (2.3.4).

The clamping mechanism (2.6.5) is assembled by screwing the clamping mechanism stud (2.6.5.2) into the hole for the clamping mechanism stud (2.6.5.1). In this case, in the cutout of the piston retainer (2.6.3), this stud (2.6.5.2) is connected to the clamping plate (2.6.5.3) by means of a threaded connection.

Take soldering equipment and assemble the working circuit (Fig. 20) from the microcontroller (3.8.1), for example, ESP 32 WROOM NodeMCU, the stepper motor driver (3.8.2), for example, tmc 2209, the four-pin male connector (3.4), for example, the GX16 aviation connector, the four-pin socket, the power sockets (3.5), for example, the DC 5.5 x 2.1 mm socket, the toggle switch (3.6), for example, KCD1-103/2P, the ground contact (3.7), for example, the M3 screw, the voltage stabilizer (4.2), for example, LM7805, the display (3.2), for example, I2C LCD Module 2004, the tact buttons (3.3), for example, KAN0611-0431B. The sequence of assembling the working circuit is shown in Fig. 20.

Take the display (3.2) and the tact buttons (3.3) and mount them in the control unit (3) from above. The power socket (3.5), the four-pin connector (3.4), the toggle switch (3.6) and the ground contact (3.7) are mounted in the control unit housing (3.1) from the rear. The power adapter (4.1), for example, a stabilized 12 V 3A power supply with a round DC 5.5 x 2.1 mm plug, is connected on one side to the power source (5), for example a 220 V network, and on the other side to the power socket (3.5).

The electrical connection between the control unit (3) and the stepper motor (1) is made, for example, by a four-wire cable (each wire goes to the contact of a separate winding of the stepper motor), which, on the side of the stepper motor (1), is connected to the contacts of the windings of the stepper motor (1.4), and on the side of the control unit (3), is connected to a four-pin pin connector (3.4).

Example 2. Providing anesthesia for an animal during an electrophysiological experiment.

To start working, connect the power adapter (4.1) to the power source (4) and to the power socket (3.5).

In this case, the control unit (3) is placed outside the experimental setup, and the injector unit (2) is located in close proximity to the animal being tested.

The control unit (3) and the stepper motor (1) are connected by a wire. In this case, it is connected from the control unit (3) side to the four-pin connector (3.4), and from the stepper motor (1) side to the contacts of the stepper motor windings (1.4).

After connecting all the wires, the device as a whole is switched on, which is done by moving the toggle switch (3.6) to the "ON" state. In this case, the indicator of the device being switched on is the appearance of the graphic interface on the display (3.2).

Calibrate the laboratory automatic syringe microinjector for the required type of syringe. This procedure is performed using a "test syringe", which is a syringe identical to the syringe that will be subsequently used to perform the injection (the test syringe is not a spare part. In fact, the operator takes two identical syringes, for example, 5 ml, with one conventionally called "test" and the other "working")

The operator draws a certain amount of liquid, such as distilled water, into the test syringe to perform calibration.

Next, the operator, using the tact buttons on the display (3.3), interacts with the graphical interface and selects the “Calibre” submenu.

There he selects the line “Move”, and then “Manual”.

After this, by pressing the tact buttons (3.3), the operator moves the carriage (2.6) to the required position to match the piston (2.7.1) of the test syringe.

The test syringe is installed with the flange (2.7.2) in the syringe flange retainer (2.3.3), and with the piston (2.7.1) in the cutout of the piston retainer (2.6.4) and fixed there with the clamping mechanism (2.6.5). This operation is performed by tightening the clamping mechanism pin (2.6.5.2) until the clamping plate (2.6.5.3) makes tight contact with the syringe piston (2.7.1).

Next, the operator selects the “Start K.” and “Start” options, after which the carriage (2.6) begins to slowly move forward, displacing the liquid from the syringe.

The operator then presses the “Stop” button on the display, the movement stops, and the program asks the operator to indicate the displaced volume of liquid.

The displaced volume of liquid is measured based on the marks on the body of the syringe or using the mass of the displaced liquid.

After specifying the obtained volume, the microcontroller (3.8.1) converts the obtained value of the liquid volume in accordance with the number of steps performed by the stepper motor (1) and records the calibration coefficient in the memory, thus the calibration process is considered complete.

In this case, if calibration has already been performed earlier and it is planned to use the same model of syringe, the operator selects “Previous K.”, and the program will use the calibration coefficient calculated during the previous calibration.

The test syringe is removed and the carriage (2.6) is moved to the initial position by selecting the “To zero” item.

A “working syringe” is installed in place of the test syringe according to the algorithm described above, while in order to eliminate errors, air is first displaced from the dead space of the syringe.

The substance is drawn into the working syringe by pressing the “Draw” button in the graphical interface.

Next, the operator specifies the required volume and speed of the drug, for example, an anesthetic substance.

Configure two actions in the Actions submenu of the graphical interface.

In this case, the operator sets the required volume, time and rate of administration for each action: the first stage is the administration of a part of the collected volume, for example, 1/10 of the part, at the maximum speed for induction of anesthesia, the second stage is the administration of the remaining volume over two hours.

After completing the specified setting and pressing the “Start” item, all specified actions are executed in the order they were specified.

In this case, in order to eliminate electromagnetic interference when recording an electrical signal, for example, from excitable tissues of an animal, using equipment for conducting an electrophysiological experiment, the ground contact (3.7) of the control unit (3) is connected to the ground of this equipment.

Next, a carrier for delivering the drug to the animal, such as a venous catheter, is installed at the end of the syringe.

Next, the operator selects the “Start” item, starting the execution of all specified actions.

In this case, the microcontroller (3.8.1) converts the specified actions and sends this information to the stepper motor driver (3.8.2), which, acting as a "gate mechanism", converts them into a sequence of pulses sent from the power adapter (4.1) to the stepper motor windings. This causes the rotor of the stepper motor (1.2) to rotate, which is transmitted through the coupling (2.5.2) to the threaded stud (2.5) and then by means of the connecting cylinder (2.6.3) to the carriage (2.6). The rotation of the latter is limited by the joint formed by the guide half rings (2.6.2) and the guide cylinders (2.4). Due to this, the linear movement of the carriage (2.6) occurs at a speed specified by the operator for a certain distance corresponding to the set injection volume.

After the first action is completed, the next action begins to be executed according to the same principle.

From the above, one can make a logical conclusion that the applicant has solved the technical problem and achieved the stated technical result - a high-precision heater for flowing liquid has been developed, eliminating the shortcomings of analogues, namely, the following has been achieved:

1 - the possibility of automatic short and long injections of medicinal preparations is provided, both at high and low injection rates with a low discrete interval, due to the use of the rotation of the rotor of the stepper motor as the driving force;

2 - the possibility of implementing multi-stage injections is provided by a programmable control unit and a graphical interface;

3 - the ability to automatically and programmatically dial the required volume of the drug at a certain speed is provided, which reduces its consumption, due to the two-sided system of fastening syringes in the injector block and the functionality of the graphical interface;

4 - the possibility of semi-automatic calibration for a specific syringe is provided, which increases the accuracy of injections, due to the use of a stepper motor and the functionality of the graphical interface;

5 - the possibility of using the device during biological studies is ensured

experiments and surgical operations on animals, which is achieved through a syringe attachment system designed for both large (5 ml) and small volume (0.5 ml) syringes;

6 - the possibility of using the device when conducting electrophysiological experiments on animals is ensured, which is achieved due to the absence of electromagnetic interference, eliminated by grounding the control unit through the ground contact.

Claims (8)

1. Laboratory automatic syringe microinjector, consisting of a stepper motor, injector unit, control unit, power source, graphic interface; the stepper motor consists of a housing, a rotor, holes for screws and winding contacts; the rotor of the stepper motor is located inside its housing in the center; the holes for the screws of the stepper motor are located on its front edge, when fixing the stepper motor in the injector block, the contacts of the windings of the stepper motor are located on its side edge; the injector block consists of a rear, middle and front fragments, guide cylinders, a threaded stud, a carriage, a syringe, fixing screws and a bearing; on the rear face of the rear fragment of the injector block there is a recess for the stepper motor housing, and on the front face there is a positional recess of the rear fragment; in the center of the rear fragment there is a through hole for the stepper motor rotor, and along the edges of its front and rear faces there are through holes for the screws of the rear fragment; in the center of the middle fragment of the injector block there is a channel for the carriage, open upward; on the inner side of the walls of this channel there are recesses for the guide cylinders, and on its front and rear ends there are guide cylinder locks made in the form of elongated rings; on the middle fragment along the edges of its end extensions there are through front and rear holes for the screws of the middle fragment; on the rear face of the front fragment of the injector unit there is a positional recess of the front fragment, and on the bottom of this recess there is a recess for the bearing in the center; recesses for screws are made along the edges of the rear face of the front fragment; a syringe flange retainer is made on the upper face of the front fragment; fixing screws are installed through the rear holes for screws of the middle fragment, the holes for screws of the rear fragment and the holes for screws of the stepper motor with the possibility of fastening together the middle fragment of the injector unit, the rear fragment of the injector unit and the stepper motor housing, wherein the rear end of the middle fragment is inserted into the positional recess of the rear fragment of the injector unit, and the stepper motor housing is immersed in the recess for the stepper motor housing; fixing screws are installed through the front holes for screws of the middle fragment into the recesses for screws of the front fragment, wherein the front end of the middle fragment is inserted into the positional recess of the front fragment; the guide cylinders are located in recesses for the guide cylinders of the middle fragment and are secured there by means of guide cylinder clamps; the threaded stud is connected to the bearing by means of a cylindrical connector, and to the rotor of the stepper motor by means of a coupling, in the latter there are recesses for the threaded stud, a recess for the rotor of the stepper motor and holes for the set screws; in the central part of the carriage there is a central hole for the connecting cylinder with the possibility of connecting the carriage with the threaded stud by the connecting cylinder with the formation of a "screw-nut" transmission between them; on the side and lower edges of the carriage there are guide half rings with the possibility of forming a sliding contact with the guide cylinders; on the upper edge of the carriage there is a cutout for the piston retainer and a clamping mechanism, the latter consists of a clamping mechanism pin installed in the corresponding hole and connected to the clamping plate in the cutout of the piston retainer; the syringe is fixed in the injector block with its flange in the syringe flange retainer and the piston in the piston retainer cutout using a clamping mechanism; the bearing is located in the bearing recess on the front section of the injector block; the control unit consists of a housing, a display, tact buttons, a four-pin male connector, a power socket, a toggle switch, a ground contact and a control system; on the lower edge of the control unit housing there are holes with control unit screws with the ability to fasten its upper and lower parts; the display, which is an information output device, and tact buttons - information input devices, are mounted in the control unit on its upper edge; on the rear edge of the control unit there are a number of elements: a four-pin male connector, to which a wire is connected, going to the contacts of the stepper motor windings; a power socket, to which a power adapter is connected, coming from the power source; a toggle switch with the ability to turn on/off the power of the device; a ground contact; the control system is designed with the ability to ensure rotation of the rotor of the stepper motor and reproduction of instructions specified by the user via the tact buttons, and consists of: a microcontroller board, a stepper motor driver with the ability to transmit power from the power source to the windings of the stepper motor in a certain sequence by output contacts - using a four-pin connector, wires and a contact of the windings of the stepper motor; the power supply consists of a 12 V power adapter and a voltage stabilizer; the 12 V power adapter is connected to the control unit via a power connector with the ability to power the stepper motor windings with 12 V through its driver, as well as with the ability to power the microcontroller, clock buttons and display with 5 V through the voltage stabilizer; the graphical interface is a sequence of software windows displayed on the display, with the ability to interact with them by means of tact buttons, and is designed with the ability to perform the following operations: performing calibration for a certain type of syringe, free movement of the carriage, moving the carriage to the zero position, performing a collection of liquid into the syringe at a certain speed and a given volume, performing an injection of the contents of the syringe at a certain speed and a given volume. 2. A method for assembling a laboratory automatic syringe microinjector according to claim 1, consisting in that prefabricated rear, middle and front fragments of the injector unit, a cylindrical connector, a coupling, a control unit housing, and a carriage are taken; a stepper motor is attached to the rear fragment of the injector unit without fixing it with screws so that the contacts of the stepper motor windings are on the right, wherein the stepper motor housing is immersed in a recess for the stepper motor housing, wherein the stepper motor rotor passes through the hole for the stepper motor rotor; a connecting cylinder is inserted into the central hole of the carriage, guide half rings are fixed to the carriage; a threaded stud is screwed into the connecting cylinder up to half its length; a cylindrical connector is screwed onto the front end of this stud; the assembled structure is inserted into the channel for the carriage so that the cylindrical connector faces the front end of the middle fragment; a coupling is installed on the rotor of the stepper motor with the end containing a recess for the rotor of the stepper motor, wherein the rotor of the stepper motor is fixed in this recess by means of set screws through the holes for the set screws; the middle fragment is installed with its rear end in the positional recess of the rear fragment, wherein the free end of the threaded stud is immersed in the recess for the threaded stud in the coupling and fixed there by means of set screws through the holes for the set screws; through the rear holes for the screws of the middle fragment, the holes for the screws of the rear fragment and the holes for the screws of the stepper motor, fixing screws are screwed in, fastening together the middle fragment of the injector unit, the rear fragment of the injector unit and the housing of the stepper motor, respectively; through the locks of the guide cylinders, the guide cylinders are inserted into the recesses for the guide cylinders until they stop, whereby a sliding contact is formed between them and the guide half rings, respectively; take the bearing, press it into the bearing recess of the front fragment; install the front end of the middle fragment into the positional recess of the front fragment, wherein the front edge of the cylindrical connector enters the inner ring of the bearing; screw the fixing screws into the recesses for the screws of the front fragment through the front holes for the screws of the middle fragment; assemble the clamping mechanism by screwing the pin of the clamping mechanism into the hole for the pin of the clamping mechanism, wherein this pin is connected to the clamping plate in the cutout of the piston retainer; take soldering equipment and assemble the working circuit from the microcontroller, stepper motor driver, four-pin pin connector, power jack, toggle switch, ground contact, voltage stabilizer, display, tact buttons; take the display and tact buttons and mount them in the control unit from above; the power socket, the four-pin male connector, the toggle switch and the ground contact are mounted in the housing of the control unit at the rear; the power adapter is connected to the power source on one side and to the power socket on the other side; the electrical connection between the control unit and the stepper motor is made with a four-wire cable, with each wire going to the contact of a separate winding of the stepper motor, which is connected to the contacts of the windings of the stepper motor on the side of the stepper motor, and is connected to the four-pin male connector on the side of the control unit. 3. A method for using a laboratory automatic syringe microinjector according to claim 1, consisting in that, to start operation, a power supply adapter is connected to a power source and to a power socket, wherein the control unit is placed outside the experimental setup, and the injector unit is located in close proximity to the animal being tested; the control unit and the stepper motor are connected by a wire, wherein it is connected from the control unit side to a four-pin connector, and from the stepper motor side to the contacts of the stepper motor windings; the device as a whole is turned on, which is done by moving the toggle switch to the "ON" state, wherein the device is turned on indicator is the appearance of a graphical interface on the display; the laboratory automatic syringe microinjector is calibrated for the required type of syringe using a test syringe, which is a syringe identical to the syringe that will subsequently be used to perform the injection; the operator draws a certain amount of liquid into the test syringe for performing the calibration; then the operator, using the tact buttons on the display, interacts with the graphical interface and selects the "Calibre" submenu, then selects the "Movement" line, then "Manual"; after that, pressing the tact buttons, the operator moves the carriage to the required position to match the piston of the test syringe; the test syringe is installed with the flange in the syringe flange retainer, and with the piston in the cutout of the piston retainer, and fixed there with the clamping mechanism, this operation is performed by tightening the pin of the clamping mechanism until the moment of tight contact of the clamping plate with the syringe piston; then the operator selects the "Start K." and "Start" items, after which the carriage begins a slow forward movement, displacing the liquid from the syringe; then the operator presses the "Stop" button on the display, the movement stops, and the program asks the operator to indicate the displaced volume of liquid; the displaced volume of liquid is measured based on the marks on the body of the syringe or using the mass of the displaced liquid; After specifying the obtained volume, the microcontroller converts the obtained value of the liquid volume in accordance with the number of steps performed by the stepper motor and writes the calibration coefficient into the memory, thus, the calibration process is considered complete; in this case, if the calibration has already been performed earlier and it is planned to use the same model of syringe, the operator selects "Previous K.", and the program will use the calibration coefficient calculated during the previous calibration; the test syringe is removed and the carriage is moved to the initial position by selecting the "To zero" item; a working syringe is installed in place of the test syringe according to the algorithm described above, while in order to exclude errors, air is first displaced from the dead space of the syringe; the substance is drawn into the working syringe by pressing the "Draw" item button on the graphical interface; then the operator specifies the required volume and speed of drawing up the drug; two actions are configured in the "Actions" submenu of the graphical interface, with the operator setting the required volume, time, and rate of administration for each action: the first stage is the administration of a portion of the collected volume at the maximum rate for inducing anesthesia, the second stage is the administration of the remaining volume over the course of two hours; after completing the specified configuration and pressing the "Start" item, all specified actions are executed in the order they are specified; in this case, in order to eliminate electromagnetic interference when recording an electrical signal by the equipment for conducting an electrophysiological experiment, the ground contact of the control unit is connected to the ground of this equipment; then a carrier for delivering the drug to the animal is installed at the end of the syringe; then the operator selects the "Start" item, starting the execution of all specified actions, with the microcontroller converting the specified actions and sending this information to the stepper motor driver, which, acting as a "gate mechanism", converts them into a sequence of pulses sent from the power adapter to the stepper motor windings, this causes the stepper motor rotor to rotate, which is transmitted through the coupling to the threaded stud and then through the connecting cylinder - to the carriage; the rotation of the latter is limited by the joint formed by the guide half rings and guide cylinders, due to which the linear movement of the carriage occurs at a speed set by the operator over a certain distance corresponding to the set injection volume; after the completion of the first action, the execution of the next action begins according to the same principle.